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The map label for this gene is potF [H]

Identifier: 83592354

GI number: 83592354

Start: 1206643

End: 1207752

Strand: Reverse

Name: potF [H]

Synonym: Rru_A1018

Alternate gene names: 83592354

Gene position: 1207752-1206643 (Counterclockwise)

Preceding gene: 83592356

Following gene: 83592353

Centisome position: 27.75

GC content: 60.18

Gene sequence:

>1110_bases
ATGCCGTCACCTGTTCTGCGCGCCTTGAGTGGTGTTGCCGTTGCCCTGCTGATCGCCGGCCCGGCCGCAGCCGCCGAGGA
CAAGGTTCTCAACGTCTATAACTGGTCCGATTACATCGCCGAGGACACCTTGGCGAAGTTCCAGGCCGAGACCGGCATCA
AGGTCAATTACGACGTTTACGACAGCAACGAGTTGCTGGAATCGAAGCTTCTGGCCGGCTCTTCGGGCTACGACATCGTC
GTTCCCACCGACCACACCCTGTCGCGGCTGATCCAGGCCGGCATTGTTCAGAAGCTCGACAAGGCGAAGATCCCCAATCT
CGCCAATCTCGACCCCAAGCTGACCGCCCGCGCCGCCGCCTATGACGCCGACAACGCCCATTCCGTTATTTACGAATGGG
GCACCAATGGCCTCGGCATCAATATCGACAAGGTGCGCGCCGCCCTCGGCCCCGACGTGAAGCTTGATTCCTTCGATCTT
CTGTTCAAGCCGGAATACGCCGAGAAGCTGGCGTCTTGCGGGATCCACGTTCTCGACAGCGCCGCCGAGGTCTTCTCGGT
CGCCCGCAATTACCTGGGACTGGAGCCCAATTCCACCGATCCCAAGGATCTGGAAAAGGCCACGGCGCTGTGGATGGGCG
TGCGCCCCTATATCCAGAAGTTCCACTCCTCGGAATTCATCAACGCCCTGGCCAATGGCGACATCTGCGTGGCCTTCGGC
TATTCGGGCGATATCTTCCAGGCCCAGGCCCAGGCCCGGGCCAAGGAAGCCGCCAATGGCGTGAAGATCGATTACATCAT
TCCCAAAGAGGGCACGCTGATCTGGTTTGATCTGATGGCCGTTCCCGCCGATGCCCCCCACCCGGAAAACGCCCATAAGT
TCATCGACTTCATTCTTCAGCCGCAGATCTCGGCCGATATCTCGAACTACGTCTGGTACGCCAATGCCAACAGCAAGGCG
ACCGAGCTGCTCGACGAGGCGGTGCGGACCAATCCGTCGATCTATCCGACCCAGGAGGTGATGGACCGCCTGTTCATCCA
GAAGACGGCGTCGCCCGAAATGGAGCGCCTGCGCACCCGCGCCTGGAACAAGATCAAAACCGGCATCTAA

Upstream 100 bases:

>100_bases
TTTCCTTTTGCGAATCAGTCACCCATACACCGTCCCGTCGTGCATTTTTCGTCCGTATAACCGGGTTTTCATCACATAGC
CCATTGAAGGGGAGGTCGCG

Downstream 100 bases:

>100_bases
GCTTTCGGTTTGATCTCCAACACATCCGCTTTCGCCCGGTACCCGCGCTCTGTCGACGGGTACCGGGTCTGTCTTCCCGC
GTAAGGGGAACGGGCCCGAT

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MPSPVLRALSGVAVALLIAGPAAAAEDKVLNVYNWSDYIAEDTLAKFQAETGIKVNYDVYDSNELLESKLLAGSSGYDIV
VPTDHTLSRLIQAGIVQKLDKAKIPNLANLDPKLTARAAAYDADNAHSVIYEWGTNGLGINIDKVRAALGPDVKLDSFDL
LFKPEYAEKLASCGIHVLDSAAEVFSVARNYLGLEPNSTDPKDLEKATALWMGVRPYIQKFHSSEFINALANGDICVAFG
YSGDIFQAQAQARAKEAANGVKIDYIIPKEGTLIWFDLMAVPADAPHPENAHKFIDFILQPQISADISNYVWYANANSKA
TELLDEAVRTNPSIYPTQEVMDRLFIQKTASPEMERLRTRAWNKIKTGI

Sequences:

>Translated_369_residues
MPSPVLRALSGVAVALLIAGPAAAAEDKVLNVYNWSDYIAEDTLAKFQAETGIKVNYDVYDSNELLESKLLAGSSGYDIV
VPTDHTLSRLIQAGIVQKLDKAKIPNLANLDPKLTARAAAYDADNAHSVIYEWGTNGLGINIDKVRAALGPDVKLDSFDL
LFKPEYAEKLASCGIHVLDSAAEVFSVARNYLGLEPNSTDPKDLEKATALWMGVRPYIQKFHSSEFINALANGDICVAFG
YSGDIFQAQAQARAKEAANGVKIDYIIPKEGTLIWFDLMAVPADAPHPENAHKFIDFILQPQISADISNYVWYANANSKA
TELLDEAVRTNPSIYPTQEVMDRLFIQKTASPEMERLRTRAWNKIKTGI
>Mature_368_residues
PSPVLRALSGVAVALLIAGPAAAAEDKVLNVYNWSDYIAEDTLAKFQAETGIKVNYDVYDSNELLESKLLAGSSGYDIVV
PTDHTLSRLIQAGIVQKLDKAKIPNLANLDPKLTARAAAYDADNAHSVIYEWGTNGLGINIDKVRAALGPDVKLDSFDLL
FKPEYAEKLASCGIHVLDSAAEVFSVARNYLGLEPNSTDPKDLEKATALWMGVRPYIQKFHSSEFINALANGDICVAFGY
SGDIFQAQAQARAKEAANGVKIDYIIPKEGTLIWFDLMAVPADAPHPENAHKFIDFILQPQISADISNYVWYANANSKAT
ELLDEAVRTNPSIYPTQEVMDRLFIQKTASPEMERLRTRAWNKIKTGI

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=363, Percent_Identity=51.7906336088154, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1787367, Length=321, Percent_Identity=34.2679127725857, Blast_Score=187, Evalue=9e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40449; Mature: 40318

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPVLRALSGVAVALLIAGPAAAAEDKVLNVYNWSDYIAEDTLAKFQAETGIKVNYDVY
CCCHHHHHHHHHHEEHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEEEEC
DSNELLESKLLAGSSGYDIVVPTDHTLSRLIQAGIVQKLDKAKIPNLANLDPKLTARAAA
CCHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
YDADNAHSVIYEWGTNGLGINIDKVRAALGPDVKLDSFDLLFKPEYAEKLASCGIHVLDS
CCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEECCEEEEECCHHHHHHHHCCHHHHHH
AAEVFSVARNYLGLEPNSTDPKDLEKATALWMGVRPYIQKFHSSEFINALANGDICVAFG
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
YSGDIFQAQAQARAKEAANGVKIDYIIPKEGTLIWFDLMAVPADAPHPENAHKFIDFILQ
CCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHC
PQISADISNYVWYANANSKATELLDEAVRTNPSIYPTQEVMDRLFIQKTASPEMERLRTR
CCHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
AWNKIKTGI
HHHHHHCCC
>Mature Secondary Structure 
PSPVLRALSGVAVALLIAGPAAAAEDKVLNVYNWSDYIAEDTLAKFQAETGIKVNYDVY
CCHHHHHHHHHHEEHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEEEEC
DSNELLESKLLAGSSGYDIVVPTDHTLSRLIQAGIVQKLDKAKIPNLANLDPKLTARAAA
CCHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
YDADNAHSVIYEWGTNGLGINIDKVRAALGPDVKLDSFDLLFKPEYAEKLASCGIHVLDS
CCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEECCEEEEECCHHHHHHHHCCHHHHHH
AAEVFSVARNYLGLEPNSTDPKDLEKATALWMGVRPYIQKFHSSEFINALANGDICVAFG
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
YSGDIFQAQAQARAKEAANGVKIDYIIPKEGTLIWFDLMAVPADAPHPENAHKFIDFILQ
CCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHC
PQISADISNYVWYANANSKATELLDEAVRTNPSIYPTQEVMDRLFIQKTASPEMERLRTR
CCHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
AWNKIKTGI
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]