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The map label for this gene is pleD [H]

Identifier: 83592321

GI number: 83592321

Start: 1172317

End: 1173843

Strand: Direct

Name: pleD [H]

Synonym: Rru_A0985

Alternate gene names: 83592321

Gene position: 1172317-1173843 (Clockwise)

Preceding gene: 83592320

Following gene: 83592322

Centisome position: 26.93

GC content: 59.59

Gene sequence:

>1527_bases
ATGCAGATAAAGCCGGAAAGGACGCTGCTTTCCGCCGAGGCGACTCTGATCGCTAGCGGTACGATTGGTGTCATCGCCAT
TCTTGGTCTTGTTACGGTCCTGCTGGCCAACTTGTGGAAAGACACCACCCGGGATGCCGAACGGTCGGCGTCGAATCTCA
TTCGGTTGATCGACGCCGATGTCCGCCGCACCATCGAACTGTATGATCTGGCCTTGAGCGGGATCATCGAAGCGGAAAAA
TTCAAGACCATCAAAGATCTTTCCCCCGAGGCCCGCCACGCCCTGATTTTCAGCCGGGTCGTGGCCACCCATTACATGGG
GGGGCTGCTGCGCCTGAACGCGGCGGGGGATATCATCGCCGATTCAACCTCGCTTGAGCCGCGCGTCGGCAATTTCGCCG
ATCGCGATTATTTCCAACTTCATAAAGCCGATCCCGACCTCGGTCTGCTGATCGGCCGCCCGATCAAAGTCCGCCTGAGC
GATCAGGAATGGCGCATTCCGATTACGCGGCGTGTCTCCACGGCCGATGGACGGTTTGATGGCGTTGTCATGGGGGCGAT
GCGGGTCAGCTATTTCGACCATCTGTTTGGTAAGCTCGATATCGGGTCTCAGGGCTCGATCACTTTGCTCAAAAGCGATG
GAACGCTTCTGGCGCGGCGGGGTTTGCCGGGCGAGTTGGATATGGTTGGTCGCAACGGTCTGGATTTTCCCAACTTCCAT
AGAATCATCAACGGCTACTCGAAAAATTTTTCGGGTTCTTCCGCTTATGACCATATCCGGCGGATTTATTCCTTCGCCCA
TGTCGAAAACCTGCCTTTGATCGTTGTCGTCGCCTTATCGGCAAACGAGGTTTATGGCGGCTGGATCCAGGCCGCCATCG
TGGTCGGCGGCATCACGTTTGTGCTGTGTTTGGGGGTTGGCGCCGTTGTCGTTTTGCTGTTGCGCGAGTTTCGCCAGCGG
CGCTTGACCGAGAGCGCCTTGAGCGAGCTGGCCGTGACCGATGGTTTGACCGGGCTGTGTAACCGCCGTCATCTTGAAGA
GGTCTTGCGGGTGGAATGCGCCCGCTCGGCGCGCAACCAGCAGCCGCTCGCCCTTTTGATGATCGATGTCGATCACTTCA
AAGCCTTCAACGATCGCCACGGCCATCGGATCGGCGACGGGGTCTTGGTCGATCTCGCCGTTATCATCCGCGCCCTATTG
AAGCGCCCCGCCGATGTCGGGGCGCGCTATGGCGGCGAGGAATTCGCCGTGATCTTGCCCGATACCGATCTTGCCGGCGG
GCTGCGGGTCGCCGAGGCGATCCGGGCGCGCATGGCCGGGCGTCCGCCCGTCGGTCAGGATCTTCGCGCCGGCACGGTCA
GCATCGGCGTCGTTTCCAGGATTCTGACGCCGGGGTATGGTGTGGATGACCTTGTCATGGACGCCGACAAGGCGCTTTAC
GCGGCAAAGGGAGCAGGGCGCAATCGCGTTGTTCCCGCCGCCGATGAAGCTGATAGGGAGGACCTGATATCTTTGAGCAC
TGAGTGA

Upstream 100 bases:

>100_bases
TTTATTCATATGGTCTAAAGGCAAGGTTGTTGCCGGGTCCCAAGCCTATAAGATCAAACGACAAGGGTCGGTGTTTTATA
GGGCGCACGGGGCTTTCGAC

Downstream 100 bases:

>100_bases
CCCTTGCCCCTTCCCCGCGCCCGAGCGTGGCCCCCAGGACCCTTTCATGATACCGGCCCTTCGCAAGATCGTTTTCACCG
GCGCGCCCTGCTCGGGCAAG

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MQIKPERTLLSAEATLIASGTIGVIAILGLVTVLLANLWKDTTRDAERSASNLIRLIDADVRRTIELYDLALSGIIEAEK
FKTIKDLSPEARHALIFSRVVATHYMGGLLRLNAAGDIIADSTSLEPRVGNFADRDYFQLHKADPDLGLLIGRPIKVRLS
DQEWRIPITRRVSTADGRFDGVVMGAMRVSYFDHLFGKLDIGSQGSITLLKSDGTLLARRGLPGELDMVGRNGLDFPNFH
RIINGYSKNFSGSSAYDHIRRIYSFAHVENLPLIVVVALSANEVYGGWIQAAIVVGGITFVLCLGVGAVVVLLLREFRQR
RLTESALSELAVTDGLTGLCNRRHLEEVLRVECARSARNQQPLALLMIDVDHFKAFNDRHGHRIGDGVLVDLAVIIRALL
KRPADVGARYGGEEFAVILPDTDLAGGLRVAEAIRARMAGRPPVGQDLRAGTVSIGVVSRILTPGYGVDDLVMDADKALY
AAKGAGRNRVVPAADEADREDLISLSTE

Sequences:

>Translated_508_residues
MQIKPERTLLSAEATLIASGTIGVIAILGLVTVLLANLWKDTTRDAERSASNLIRLIDADVRRTIELYDLALSGIIEAEK
FKTIKDLSPEARHALIFSRVVATHYMGGLLRLNAAGDIIADSTSLEPRVGNFADRDYFQLHKADPDLGLLIGRPIKVRLS
DQEWRIPITRRVSTADGRFDGVVMGAMRVSYFDHLFGKLDIGSQGSITLLKSDGTLLARRGLPGELDMVGRNGLDFPNFH
RIINGYSKNFSGSSAYDHIRRIYSFAHVENLPLIVVVALSANEVYGGWIQAAIVVGGITFVLCLGVGAVVVLLLREFRQR
RLTESALSELAVTDGLTGLCNRRHLEEVLRVECARSARNQQPLALLMIDVDHFKAFNDRHGHRIGDGVLVDLAVIIRALL
KRPADVGARYGGEEFAVILPDTDLAGGLRVAEAIRARMAGRPPVGQDLRAGTVSIGVVSRILTPGYGVDDLVMDADKALY
AAKGAGRNRVVPAADEADREDLISLSTE
>Mature_508_residues
MQIKPERTLLSAEATLIASGTIGVIAILGLVTVLLANLWKDTTRDAERSASNLIRLIDADVRRTIELYDLALSGIIEAEK
FKTIKDLSPEARHALIFSRVVATHYMGGLLRLNAAGDIIADSTSLEPRVGNFADRDYFQLHKADPDLGLLIGRPIKVRLS
DQEWRIPITRRVSTADGRFDGVVMGAMRVSYFDHLFGKLDIGSQGSITLLKSDGTLLARRGLPGELDMVGRNGLDFPNFH
RIINGYSKNFSGSSAYDHIRRIYSFAHVENLPLIVVVALSANEVYGGWIQAAIVVGGITFVLCLGVGAVVVLLLREFRQR
RLTESALSELAVTDGLTGLCNRRHLEEVLRVECARSARNQQPLALLMIDVDHFKAFNDRHGHRIGDGVLVDLAVIIRALL
KRPADVGARYGGEEFAVILPDTDLAGGLRVAEAIRARMAGRPPVGQDLRAGTVSIGVVSRILTPGYGVDDLVMDADKALY
AAKGAGRNRVVPAADEADREDLISLSTE

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87082007, Length=212, Percent_Identity=38.2075471698113, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1786584, Length=238, Percent_Identity=36.1344537815126, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI87081881, Length=211, Percent_Identity=35.0710900473934, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI145693134, Length=197, Percent_Identity=36.5482233502538, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1787262, Length=190, Percent_Identity=35.7894736842105, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787802, Length=162, Percent_Identity=39.5061728395062, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1788381, Length=182, Percent_Identity=35.1648351648352, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788085, Length=192, Percent_Identity=31.7708333333333, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787541, Length=202, Percent_Identity=28.2178217821782, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87081977, Length=185, Percent_Identity=32.4324324324324, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1787816, Length=167, Percent_Identity=34.7305389221557, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 55310; Mature: 55310

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIKPERTLLSAEATLIASGTIGVIAILGLVTVLLANLWKDTTRDAERSASNLIRLIDAD
CCCCCCHHHEECCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRRTIELYDLALSGIIEAEKFKTIKDLSPEARHALIFSRVVATHYMGGLLRLNAAGDIIA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEE
DSTSLEPRVGNFADRDYFQLHKADPDLGLLIGRPIKVRLSDQEWRIPITRRVSTADGRFD
CCCCCCCCCCCCCCCCHHEEECCCCCCEEEECCCEEEEECCCCEECCCEEEEECCCCCCC
GVVMGAMRVSYFDHLFGKLDIGSQGSITLLKSDGTLLARRGLPGELDMVGRNGLDFPNFH
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEECCCCCCEEECCCCCCCCCHHH
RIINGYSKNFSGSSAYDHIRRIYSFAHVENLPLIVVVALSANEVYGGWIQAAIVVGGITF
HHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
VLCLGVGAVVVLLLREFRQRRLTESALSELAVTDGLTGLCNRRHLEEVLRVECARSARNQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
QPLALLMIDVDHFKAFNDRHGHRIGDGVLVDLAVIIRALLKRPADVGARYGGEEFAVILP
CCEEEEEEEHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEEC
DTDLAGGLRVAEAIRARMAGRPPVGQDLRAGTVSIGVVSRILTPGYGVDDLVMDADKALY
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHCCHHHHH
AAKGAGRNRVVPAADEADREDLISLSTE
HHCCCCCCCCCCCCCCCCHHHHHCCCCC
>Mature Secondary Structure
MQIKPERTLLSAEATLIASGTIGVIAILGLVTVLLANLWKDTTRDAERSASNLIRLIDAD
CCCCCCHHHEECCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRRTIELYDLALSGIIEAEKFKTIKDLSPEARHALIFSRVVATHYMGGLLRLNAAGDIIA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEE
DSTSLEPRVGNFADRDYFQLHKADPDLGLLIGRPIKVRLSDQEWRIPITRRVSTADGRFD
CCCCCCCCCCCCCCCCHHEEECCCCCCEEEECCCEEEEECCCCEECCCEEEEECCCCCCC
GVVMGAMRVSYFDHLFGKLDIGSQGSITLLKSDGTLLARRGLPGELDMVGRNGLDFPNFH
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEECCCCCCEEECCCCCCCCCHHH
RIINGYSKNFSGSSAYDHIRRIYSFAHVENLPLIVVVALSANEVYGGWIQAAIVVGGITF
HHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
VLCLGVGAVVVLLLREFRQRRLTESALSELAVTDGLTGLCNRRHLEEVLRVECARSARNQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
QPLALLMIDVDHFKAFNDRHGHRIGDGVLVDLAVIIRALLKRPADVGARYGGEEFAVILP
CCEEEEEEEHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEEC
DTDLAGGLRVAEAIRARMAGRPPVGQDLRAGTVSIGVVSRILTPGYGVDDLVMDADKALY
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHCCHHHHH
AAKGAGRNRVVPAADEADREDLISLSTE
HHCCCCCCCCCCCCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA