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The map label for this gene is 83592319

Identifier: 83592319

GI number: 83592319

Start: 1169495

End: 1170952

Strand: Direct

Name: 83592319

Synonym: Rru_A0983

Alternate gene names: NA

Gene position: 1169495-1170952 (Clockwise)

Preceding gene: 83592318

Following gene: 83592320

Centisome position: 26.87

GC content: 69.14

Gene sequence:

>1458_bases
ATGTCCCCCGATCCCGATGATATCCTGGCCGGTGACGCCCGCGCGCTTTCGCGCCGCATCCACCACCGCGCCGTTTCCTG
CCGCGAGGTGATGGCCGCGACCCTGGCCCGCATCGATCGTTTCAACCCCGCCGTCACCGCCCTGGTGTCCTTGCGCGATC
GCGAGGATCTGTTGCGCGAGGCCGATGCCAAGGACGCCCTGCTGGGGAAGGGGCTTTCGCAAGGTCCGCTGCACGGCTTC
CCCCAGGCGCCCAAGGATCTGGCGGCCACCCGGGGCATCGCCACCACCCGGGGCTCGCCGCTGTTTCGCCACCAAATCCC
CAAGCAGGACGCGATCTTCGTCGAGCGCCTGCGCGCCGGCGGGGCGATCTTCGTCGGCAAGACCAATACCCCCGAATTCG
GCCTGGGGTCGCAGACCTATAACCCGGTCTTCGGGGCGACGGGCAACGCCTATGATCCGCGGCTGACCGCCGGCGGCAGC
AGTGGCGGGGCGGCCTGCGCCCTGGCCCTGGGCATGCTTGCCGTGGCCGACGGCAGCGACATGATGGGATCGCTGCGCAA
TCCAGCGGCCTTCAACAACGTGTTCGGCTTCCGTCCCTCGGCCGGTCGCGTGCCCCATGGCGTTGTTCCTGATTTGTTCT
TTCAGCAACTGGGCTATGACGGGCCGATGGGGCGCAGCGTGGCCGATCTGGCGCTGTTGCTCTCGGTGATGGCCGGCTTC
GACGCCCGGGCGCCGCAGTCGCTGGCCGGGGATCCCGCGGTTTTCGCCCAGCCCCTGGAGGGTTCGGTCGCCGGGGTTCG
TGTCGGCTGGCTGGGTGATCTTGGCGGCTATCTGGCCTTCGAGCCGGGGATCTTGGAGCTGTGCCAGGGCGCCTTGGCGA
CCCTGGGCGATCTCGGCTGCGTCGTTGACGAGGCGACGGCCGGTTTTCCCCTGGATGCCCTGTGGACGGCCTGGACCGAT
CTGCGCAGCTTTCTGGTGGCCGGTGATCTGGGTCCGCTCTATGCCGATCCGGTCAAACGCGCCCAGATGAAGCCCGAGGC
GATTTTCGAGATCGAGCGCGGGCTGGCTTTGAGCGGTCCGCAGGTTTTCGCCGCCTCGGCCCGGCGCAGCGCCTGGTACC
GGCGGATGCTGGTGCTGTTCGAGCGCTTCGATGTGCTGGCCCTGCCCAGCGCCCAGGTCTTTCCCTTTGAGCATGCCACG
CCCTGGCCGCGCGCCATCGCTGGCCGGACGATGGACAGCTATCACCGCTGGATGGAGGTGGTGGTGCCCGCCAGCATGGC
CGGGGTGCCGGTGATCAGCGTTCCCGTCGGCTTCAACGCCGCCGGCTTGCCGATGGGCCTGCAGGTGATCGGCAAGCCGC
GCGACGACCTGTCGCTGCTGCGCTTGGCCCATGCCTATGATCTGGCCACCCGCTGGCCCGCCCGGCGGCCGCCGCCGGTT
CTTTCCCCCGCGTCTTGA

Upstream 100 bases:

>100_bases
CGATCCGCGACGTGTTCTTCGCCCCCCGTCATCCCTATACGCGAAAACTGCTGGCCGCCTCGCCGGGCCGCGATTTCGCC
TTCGCCCGGTGAGAAGCGCC

Downstream 100 bases:

>100_bases
AGGGTTCTCAAACCATGTCCGTCATCCCGCAGATCGCCGCCTTCGCCCAGGAGCTTGGCGCCCTTCGCCACGATATCCAC
GCCCACCCCGAACTGGGCTT

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MSPDPDDILAGDARALSRRIHHRAVSCREVMAATLARIDRFNPAVTALVSLRDREDLLREADAKDALLGKGLSQGPLHGF
PQAPKDLAATRGIATTRGSPLFRHQIPKQDAIFVERLRAGGAIFVGKTNTPEFGLGSQTYNPVFGATGNAYDPRLTAGGS
SGGAACALALGMLAVADGSDMMGSLRNPAAFNNVFGFRPSAGRVPHGVVPDLFFQQLGYDGPMGRSVADLALLLSVMAGF
DARAPQSLAGDPAVFAQPLEGSVAGVRVGWLGDLGGYLAFEPGILELCQGALATLGDLGCVVDEATAGFPLDALWTAWTD
LRSFLVAGDLGPLYADPVKRAQMKPEAIFEIERGLALSGPQVFAASARRSAWYRRMLVLFERFDVLALPSAQVFPFEHAT
PWPRAIAGRTMDSYHRWMEVVVPASMAGVPVISVPVGFNAAGLPMGLQVIGKPRDDLSLLRLAHAYDLATRWPARRPPPV
LSPAS

Sequences:

>Translated_485_residues
MSPDPDDILAGDARALSRRIHHRAVSCREVMAATLARIDRFNPAVTALVSLRDREDLLREADAKDALLGKGLSQGPLHGF
PQAPKDLAATRGIATTRGSPLFRHQIPKQDAIFVERLRAGGAIFVGKTNTPEFGLGSQTYNPVFGATGNAYDPRLTAGGS
SGGAACALALGMLAVADGSDMMGSLRNPAAFNNVFGFRPSAGRVPHGVVPDLFFQQLGYDGPMGRSVADLALLLSVMAGF
DARAPQSLAGDPAVFAQPLEGSVAGVRVGWLGDLGGYLAFEPGILELCQGALATLGDLGCVVDEATAGFPLDALWTAWTD
LRSFLVAGDLGPLYADPVKRAQMKPEAIFEIERGLALSGPQVFAASARRSAWYRRMLVLFERFDVLALPSAQVFPFEHAT
PWPRAIAGRTMDSYHRWMEVVVPASMAGVPVISVPVGFNAAGLPMGLQVIGKPRDDLSLLRLAHAYDLATRWPARRPPPV
LSPAS
>Mature_484_residues
SPDPDDILAGDARALSRRIHHRAVSCREVMAATLARIDRFNPAVTALVSLRDREDLLREADAKDALLGKGLSQGPLHGFP
QAPKDLAATRGIATTRGSPLFRHQIPKQDAIFVERLRAGGAIFVGKTNTPEFGLGSQTYNPVFGATGNAYDPRLTAGGSS
GGAACALALGMLAVADGSDMMGSLRNPAAFNNVFGFRPSAGRVPHGVVPDLFFQQLGYDGPMGRSVADLALLLSVMAGFD
ARAPQSLAGDPAVFAQPLEGSVAGVRVGWLGDLGGYLAFEPGILELCQGALATLGDLGCVVDEATAGFPLDALWTAWTDL
RSFLVAGDLGPLYADPVKRAQMKPEAIFEIERGLALSGPQVFAASARRSAWYRRMLVLFERFDVLALPSAQVFPFEHATP
WPRAIAGRTMDSYHRWMEVVVPASMAGVPVISVPVGFNAAGLPMGLQVIGKPRDDLSLLRLAHAYDLATRWPARRPPPVL
SPAS

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=251, Percent_Identity=35.0597609561753, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI222831590, Length=533, Percent_Identity=27.0168855534709, Blast_Score=109, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17537465, Length=506, Percent_Identity=25.8893280632411, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17543272, Length=458, Percent_Identity=26.8558951965066, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17538252, Length=284, Percent_Identity=28.169014084507, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17556264, Length=307, Percent_Identity=28.3387622149837, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=227, Percent_Identity=26.8722466960352, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17556276, Length=274, Percent_Identity=28.4671532846715, Blast_Score=66, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17556278, Length=274, Percent_Identity=28.4671532846715, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=458, Percent_Identity=28.1659388646288, Blast_Score=116, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6320448, Length=278, Percent_Identity=27.6978417266187, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323950, Length=188, Percent_Identity=31.3829787234043, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24648435, Length=500, Percent_Identity=27.6, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24648437, Length=500, Percent_Identity=27.6, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI45550774, Length=513, Percent_Identity=27.4853801169591, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24652985, Length=252, Percent_Identity=34.9206349206349, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI19922090, Length=252, Percent_Identity=34.9206349206349, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24652981, Length=252, Percent_Identity=34.9206349206349, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24652983, Length=252, Percent_Identity=34.9206349206349, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24644968, Length=493, Percent_Identity=24.3407707910751, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI161078093, Length=513, Percent_Identity=27.8752436647173, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648441, Length=429, Percent_Identity=27.2727272727273, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648439, Length=429, Percent_Identity=27.2727272727273, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21356731, Length=248, Percent_Identity=28.6290322580645, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24648113, Length=188, Percent_Identity=35.6382978723404, Blast_Score=85, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 51510; Mature: 51379

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPDPDDILAGDARALSRRIHHRAVSCREVMAATLARIDRFNPAVTALVSLRDREDLLRE
CCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
ADAKDALLGKGLSQGPLHGFPQAPKDLAATRGIATTRGSPLFRHQIPKQDAIFVERLRAG
CCHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHCC
GAIFVGKTNTPEFGLGSQTYNPVFGATGNAYDPRLTAGGSSGGAACALALGMLAVADGSD
CEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCHH
MMGSLRNPAAFNNVFGFRPSAGRVPHGVVPDLFFQQLGYDGPMGRSVADLALLLSVMAGF
HHHHHCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCC
DARAPQSLAGDPAVFAQPLEGSVAGVRVGWLGDLGGYLAFEPGILELCQGALATLGDLGC
CCCCCHHHCCCCHHCCCCCCCCCCEEEEECHHCCCCCEEECCCHHHHHHHHHHHHHHHHH
VVDEATAGFPLDALWTAWTDLRSFLVAGDLGPLYADPVKRAQMKPEAIFEIERGLALSGP
HHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCHHHHHHCCHHHHHHHHCCCCCCCC
QVFAASARRSAWYRRMLVLFERFDVLALPSAQVFPFEHATPWPRAIAGRTMDSYHRWMEV
HHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHH
VVPASMAGVPVISVPVGFNAAGLPMGLQVIGKPRDDLSLLRLAHAYDLATRWPARRPPPV
HHCHHHCCCCEEEECCCCCCCCCCCCHHEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
LSPAS
CCCCC
>Mature Secondary Structure 
SPDPDDILAGDARALSRRIHHRAVSCREVMAATLARIDRFNPAVTALVSLRDREDLLRE
CCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
ADAKDALLGKGLSQGPLHGFPQAPKDLAATRGIATTRGSPLFRHQIPKQDAIFVERLRAG
CCHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHCC
GAIFVGKTNTPEFGLGSQTYNPVFGATGNAYDPRLTAGGSSGGAACALALGMLAVADGSD
CEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCHH
MMGSLRNPAAFNNVFGFRPSAGRVPHGVVPDLFFQQLGYDGPMGRSVADLALLLSVMAGF
HHHHHCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCC
DARAPQSLAGDPAVFAQPLEGSVAGVRVGWLGDLGGYLAFEPGILELCQGALATLGDLGC
CCCCCHHHCCCCHHCCCCCCCCCCEEEEECHHCCCCCEEECCCHHHHHHHHHHHHHHHHH
VVDEATAGFPLDALWTAWTDLRSFLVAGDLGPLYADPVKRAQMKPEAIFEIERGLALSGP
HHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCHHHHHHCCHHHHHHHHCCCCCCCC
QVFAASARRSAWYRRMLVLFERFDVLALPSAQVFPFEHATPWPRAIAGRTMDSYHRWMEV
HHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHH
VVPASMAGVPVISVPVGFNAAGLPMGLQVIGKPRDDLSLLRLAHAYDLATRWPARRPPPV
HHCHHHCCCCEEEECCCCCCCCCCCCHHEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
LSPAS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]