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The map label for this gene is amiD [H]

Identifier: 83592296

GI number: 83592296

Start: 1145882

End: 1146607

Strand: Reverse

Name: amiD [H]

Synonym: Rru_A0960

Alternate gene names: 83592296

Gene position: 1146607-1145882 (Counterclockwise)

Preceding gene: 83592297

Following gene: 83592295

Centisome position: 26.34

GC content: 65.84

Gene sequence:

>726_bases
GTGGCCAGCCGTCCCGCCCCCCCGGATTTCCCGGTTATCGAGGCCCTCTCGCCCAATGCCGATGACCGCCCGCCCGGGCA
GATCATCGACATGCTGGTGATCCATTACACCGGCATGCCCAGCGCGCAGGCGGCCCTGGCGCGCCTCCTCGATCCCCAGG
CCCAGGTCAGCGCCCACTGGCTGATCGACGAGGATGGAACGGCCTATAAGCTGGTCGAGGAACGCCGCCGGGCCTGGCAC
GCCGGCGTCTCGGCCTGGGGCGGCCGCCCAGGGGTCAATGCCCGCTCGATCGGCATCGAATTGGTCAATCCCGGCCACGA
ATTCGGCTACCGCCCCTTCCCCGAGGCCCAGATGACCACCCTGATCGCCCTTGGCCGGGGTATTCTGGAGCGCCATCCCA
TCCCCGCGGCCCATGTCGTCGGCCATGCCGATGTCGCCCCGACCCGCAAGGAGGACCCGGGAGAACTGTTCGACTGGCAG
AGACTCGCCTCAAACGGCATCGGCCGCTGGCCGCCCCCACCCTTGGGCGTCCGCCCGGAGACGGTTGACGCGACCGAGGG
CGAAGCGGCCTGCCTTCTTGAACAAATAGGCTATGATATCAGTTCCTTACAAGCCACTGTTCTCGCCTTTCAGCGTCACT
TCCGCCCAAAGCGCCTCGATGGTCGTATCGACTCGGAAACGCTTTGGCTAATCAAGGCTCTTGCCCGTGACGTTCAAGCC
CATTAA

Upstream 100 bases:

>100_bases
AATGCCCCCCGATTTCGTCGATCTCGCCAACAAGAAAATGGCGACCATCAACGCCGCCTACGAGACCCTGACCAAGGCCC
GGGCCGGGCGCTGAAGGCCC

Downstream 100 bases:

>100_bases
GGTCGCCTTCGACCGCCAGTCGGCCGGATGGCCGCTCTCCGTCATCGTCCCCGGGCCACCCCCGATGGACCGTCGGGGGG
AGGAAAGTCCGGGCTCCACG

Product: AmpD (negative regulator of AmpC)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MASRPAPPDFPVIEALSPNADDRPPGQIIDMLVIHYTGMPSAQAALARLLDPQAQVSAHWLIDEDGTAYKLVEERRRAWH
AGVSAWGGRPGVNARSIGIELVNPGHEFGYRPFPEAQMTTLIALGRGILERHPIPAAHVVGHADVAPTRKEDPGELFDWQ
RLASNGIGRWPPPPLGVRPETVDATEGEAACLLEQIGYDISSLQATVLAFQRHFRPKRLDGRIDSETLWLIKALARDVQA
H

Sequences:

>Translated_241_residues
MASRPAPPDFPVIEALSPNADDRPPGQIIDMLVIHYTGMPSAQAALARLLDPQAQVSAHWLIDEDGTAYKLVEERRRAWH
AGVSAWGGRPGVNARSIGIELVNPGHEFGYRPFPEAQMTTLIALGRGILERHPIPAAHVVGHADVAPTRKEDPGELFDWQ
RLASNGIGRWPPPPLGVRPETVDATEGEAACLLEQIGYDISSLQATVLAFQRHFRPKRLDGRIDSETLWLIKALARDVQA
H
>Mature_240_residues
ASRPAPPDFPVIEALSPNADDRPPGQIIDMLVIHYTGMPSAQAALARLLDPQAQVSAHWLIDEDGTAYKLVEERRRAWHA
GVSAWGGRPGVNARSIGIELVNPGHEFGYRPFPEAQMTTLIALGRGILERHPIPAAHVVGHADVAPTRKEDPGELFDWQR
LASNGIGRWPPPPLGVRPETVDATEGEAACLLEQIGYDISSLQATVLAFQRHFRPKRLDGRIDSETLWLIKALARDVQAH

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=229, Percent_Identity=39.3013100436681, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1786300, Length=163, Percent_Identity=37.4233128834356, Blast_Score=98, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 26433; Mature: 26302

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASRPAPPDFPVIEALSPNADDRPPGQIIDMLVIHYTGMPSAQAALARLLDPQAQVSAHW
CCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHCEEEE
LIDEDGTAYKLVEERRRAWHAGVSAWGGRPGVNARSIGIELVNPGHEFGYRPFPEAQMTT
EECCCCCHHHHHHHHHHHHHHCHHHCCCCCCCCCEEEEEEEECCCHHCCCCCCCHHHHHH
LIALGRGILERHPIPAAHVVGHADVAPTRKEDPGELFDWQRLASNGIGRWPPPPLGVRPE
HHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
TVDATEGEAACLLEQIGYDISSLQATVLAFQRHFRPKRLDGRIDSETLWLIKALARDVQA
CCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
H
C
>Mature Secondary Structure 
ASRPAPPDFPVIEALSPNADDRPPGQIIDMLVIHYTGMPSAQAALARLLDPQAQVSAHW
CCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHCEEEE
LIDEDGTAYKLVEERRRAWHAGVSAWGGRPGVNARSIGIELVNPGHEFGYRPFPEAQMTT
EECCCCCHHHHHHHHHHHHHHCHHHCCCCCCCCCEEEEEEEECCCHHCCCCCCCHHHHHH
LIALGRGILERHPIPAAHVVGHADVAPTRKEDPGELFDWQRLASNGIGRWPPPPLGVRPE
HHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
TVDATEGEAACLLEQIGYDISSLQATVLAFQRHFRPKRLDGRIDSETLWLIKALARDVQA
CCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
H
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]