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The map label for this gene is rffG [C]

Identifier: 83592271

GI number: 83592271

Start: 1114135

End: 1115001

Strand: Direct

Name: rffG [C]

Synonym: Rru_A0935

Alternate gene names: 83592271

Gene position: 1114135-1115001 (Clockwise)

Preceding gene: 83592270

Following gene: 83592272

Centisome position: 25.6

GC content: 54.79

Gene sequence:

>867_bases
ATGAATCCCCTCTATGGGGGGAACATGCATAATATCGTCGATATAAAAGACAAGGTGACGGTCAATATCTCCGATGTCCG
CGATCGCTACAGCCTGCCGTGGCTGGTGACGGGAAAGGATTTCATCTTCAATCTGGCCGGTCAGACCAGCCATATGGATT
CGATGAGCGATCCCTTCACCGATCTGGAAATCAACGCCAAATCACAATTGTCGCTGCTTGAGGTTTGTCGGGCCCATTGC
CCGGAAACGAAAATCGTTTTCGCCTCGACCCGCCAGATTTACGGGCGGCCCCAGTATCTGCCGGTGGATGAGCGCCATCC
GATTAAACCCGTGGACGTGAACGGCATCAATAAGGCGGCGGGGGAATTCTACCATATCCTCTATAGCGAGGTTTATGGCT
TGCGGACCACCGTGCTGCGGCTGACCAATACCTATGGGCCACGGATGCGGGTCAAGGACGCCCGCCAAACCTTCCTGGGG
ATCTGGTTTCGCAAACTGCTGGAAGGTCAGTCCTTCGAGGTGTGGGGCGGCGACCAGAAGCGCGATTACACTTATATCGA
TGATCTGGTCGATGCCCTGATGATCTCGACGCTTGATCCGGCCTGCGACGGCAAGACCTATAACATCGGCGGCAGCGAAA
TCCTGTCTTTGCGCGAGACGGCCGAGATGGTGATCGGCAATCGTCCGGGGGCGACTTATGAAACCCGCGTCTATCCCGAG
GACCGCAAGAAGATAGATATCGGCGATTATTATTCCGACTATCGGCTTTTCGAAACGGCGACCGGGTGGAGCCCCAAGGT
CGGTTTCGTGGAAGGGTCGAAAAGGACCTTGGACTATTACCGGGACAATCTTTCCTATTATCTGTAG

Upstream 100 bases:

>100_bases
GGGACGAAACGCCCTCATCATTGGCGGCCTGGGCTTCATCGGCTCCAATCTGGCGCGGCGTCTGGTTGATCTGGGGGCTT
ATGTCACCCTGATCGACAGC

Downstream 100 bases:

>100_bases
AGAGCACGGGAAAATGCGGTTTCTGATTGTCGATACGGTTTATCCCGGCTTTTTAAGCTGGTTGTATAACGAACGCTCTC
CCGGTCTGGCGGCGAAGTCC

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: Galactowaldenase; UDP-galactose 4-epimerase [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MNPLYGGNMHNIVDIKDKVTVNISDVRDRYSLPWLVTGKDFIFNLAGQTSHMDSMSDPFTDLEINAKSQLSLLEVCRAHC
PETKIVFASTRQIYGRPQYLPVDERHPIKPVDVNGINKAAGEFYHILYSEVYGLRTTVLRLTNTYGPRMRVKDARQTFLG
IWFRKLLEGQSFEVWGGDQKRDYTYIDDLVDALMISTLDPACDGKTYNIGGSEILSLRETAEMVIGNRPGATYETRVYPE
DRKKIDIGDYYSDYRLFETATGWSPKVGFVEGSKRTLDYYRDNLSYYL

Sequences:

>Translated_288_residues
MNPLYGGNMHNIVDIKDKVTVNISDVRDRYSLPWLVTGKDFIFNLAGQTSHMDSMSDPFTDLEINAKSQLSLLEVCRAHC
PETKIVFASTRQIYGRPQYLPVDERHPIKPVDVNGINKAAGEFYHILYSEVYGLRTTVLRLTNTYGPRMRVKDARQTFLG
IWFRKLLEGQSFEVWGGDQKRDYTYIDDLVDALMISTLDPACDGKTYNIGGSEILSLRETAEMVIGNRPGATYETRVYPE
DRKKIDIGDYYSDYRLFETATGWSPKVGFVEGSKRTLDYYRDNLSYYL
>Mature_288_residues
MNPLYGGNMHNIVDIKDKVTVNISDVRDRYSLPWLVTGKDFIFNLAGQTSHMDSMSDPFTDLEINAKSQLSLLEVCRAHC
PETKIVFASTRQIYGRPQYLPVDERHPIKPVDVNGINKAAGEFYHILYSEVYGLRTTVLRLTNTYGPRMRVKDARQTFLG
IWFRKLLEGQSFEVWGGDQKRDYTYIDDLVDALMISTLDPACDGKTYNIGGSEILSLRETAEMVIGNRPGATYETRVYPE
DRKKIDIGDYYSDYRLFETATGWSPKVGFVEGSKRTLDYYRDNLSYYL

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=253, Percent_Identity=28.0632411067194, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI7657641, Length=291, Percent_Identity=25.7731958762887, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI48994969, Length=310, Percent_Identity=26.4516129032258, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788353, Length=313, Percent_Identity=24.6006389776358, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788589, Length=274, Percent_Identity=25.1824817518248, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17539532, Length=248, Percent_Identity=25.4032258064516, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17568069, Length=254, Percent_Identity=29.5275590551181, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI21356223, Length=257, Percent_Identity=26.8482490272374, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.2 [H]

Molecular weight: Translated: 33046; Mature: 33046

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPLYGGNMHNIVDIKDKVTVNISDVRDRYSLPWLVTGKDFIFNLAGQTSHMDSMSDPFT
CCCCCCCCCCCEEEECCEEEEEHHHHHHHHCCCEEEECHHHEEEECCCCCCCCCCCCCCC
DLEINAKSQLSLLEVCRAHCPETKIVFASTRQIYGRPQYLPVDERHPIKPVDVNGINKAA
CEEECCHHHHHHHHHHHHHCCCCEEEEEECHHHCCCCCCCCCCCCCCCCCCCCCCCCHHH
GEFYHILYSEVYGLRTTVLRLTNTYGPRMRVKDARQTFLGIWFRKLLEGQSFEVWGGDQK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEECCCCC
RDYTYIDDLVDALMISTLDPACDGKTYNIGGSEILSLRETAEMVIGNRPGATYETRVYPE
CCCHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
DRKKIDIGDYYSDYRLFETATGWSPKVGFVEGSKRTLDYYRDNLSYYL
CCCEEECCCCCCCCEEEEECCCCCCCEEEECCCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MNPLYGGNMHNIVDIKDKVTVNISDVRDRYSLPWLVTGKDFIFNLAGQTSHMDSMSDPFT
CCCCCCCCCCCEEEECCEEEEEHHHHHHHHCCCEEEECHHHEEEECCCCCCCCCCCCCCC
DLEINAKSQLSLLEVCRAHCPETKIVFASTRQIYGRPQYLPVDERHPIKPVDVNGINKAA
CEEECCHHHHHHHHHHHHHCCCCEEEEEECHHHCCCCCCCCCCCCCCCCCCCCCCCCHHH
GEFYHILYSEVYGLRTTVLRLTNTYGPRMRVKDARQTFLGIWFRKLLEGQSFEVWGGDQK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEECCCCC
RDYTYIDDLVDALMISTLDPACDGKTYNIGGSEILSLRETAEMVIGNRPGATYETRVYPE
CCCHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
DRKKIDIGDYYSDYRLFETATGWSPKVGFVEGSKRTLDYYRDNLSYYL
CCCEEECCCCCCCCEEEEECCCCCCCEEEECCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]