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The map label for this gene is mdtE [H]

Identifier: 83592223

GI number: 83592223

Start: 1064008

End: 1065204

Strand: Reverse

Name: mdtE [H]

Synonym: Rru_A0887

Alternate gene names: 83592223

Gene position: 1065204-1064008 (Counterclockwise)

Preceding gene: 83592224

Following gene: 83592222

Centisome position: 24.47

GC content: 68.25

Gene sequence:

>1197_bases
ATGACCAAGGGTTCCCGTTTCGTCAAAGGTCGCGCCGTCCTGCTGGGCACGGTTGGCCTGATCCTTCTCGCCGGCGGCGT
CGGCGTCGTTCTTGACCGGGGCGTCGGGGTGCCGGCCCAGGCCCAAGCCCCGGCCCAGGCGCCGCGGGTCGGCGTCCAAA
CCCTCACCCCGCGACAGGTTCAGGTCTGGTCGTCGTTCTCCGGACGCCTGCGCGCCGTCGATCTGGCCGAGATCCGTCCC
GAGGTCGGCGGCCGCCTCACCGAGGTCCGTTTCCGCGACGGTCAGACCGTCGCCGTCGGCGATGTGCTGTTCGTCATCGA
TCCCGCCCCTTACGAAGCGGCGATGGCCAAGGCCGAGGCCTCTTTGACCTCGGCGCGGACCAATGCGGCCTTTGCCCGGA
CCGAGCTTAACCGGGCCGTTAGCCTGGTGAAGACCCAGACCATCGCCCAGCGCCTGTATGACGAACGCGCCAATGCCAAC
CGCCTCGCCCAGGCCGCCGTTCTGGTGGCCGAGGCCGAGGTCAAGCGGGCGCGGATCGATCTGGATCATACCCGGGTCAA
GGCGCCGATCGCCGGCCGGGTCAGCCGGGCCGAAATTACCGTGGGCAATGTGGTTCAGCCCGGAGCCGGCGCGCCGCTTC
TCACCTCCATCGTCTCCAACGACGGCCTTTATGCCGACTTCGAGGTCGACGAGCAGACCTATATGTCGGGCATCCGGGCG
CGCGGTCCCTCGCGCGATCAGGAAAACCGCATCCCGGTTCAGATCACCGCCCAGGGCGACGAGGCCCATCCCTATGGCGG
CACCATCCATAGCTTCGATAATCGCATCGACAGCGCCACGGGCACCATCCGGGCGCGGGCGTTGCTGGCCAATGAGGACG
GCGCCTTGATGCCGGGCATGTTCGTCTCGGTCAAGGTGGCCAGCGGCGGCGGGGCGGGCGCCCTGATGGTGCAGGAACGG
GCCATCGGCAACGACCAGAGCAAGAAGTTCGTCTATGTCGTCGGCGAGGACGCCAAGGTCGCCTACCGCGAGGTCGACCT
CGGGGCGCGGGTCGAGGGCGAGCGCATCGTGCTTTCGGGGTTGCGGGCCGGTGAGCGGGTGATCGTCGATGGCCTGCAAC
AGGTCCGCCCGACGATGACCGTCGAGATCAAGGAAGCTGGGCTTGAGACCGCGTCACGCGCCCTGGCGGCGAAGTGA

Upstream 100 bases:

>100_bases
TTTTTGACCTCAAACTAAACTGACCGGTCAATTAATCGGGTATGGCACCCGATACCGGTCCCGATCCCGTCCTTTTCCCG
GTGCTTGCAGGAGATCCGCA

Downstream 100 bases:

>100_bases
TCTCTTTTCGTTGACCGCGCGCCCGGAAAGGCCCCGCCATGTCGCTCTCCAAATTCTTCATCGATCGTCCGATCTTCGCC
GGCGTGATCTCGACGGTCAT

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MTKGSRFVKGRAVLLGTVGLILLAGGVGVVLDRGVGVPAQAQAPAQAPRVGVQTLTPRQVQVWSSFSGRLRAVDLAEIRP
EVGGRLTEVRFRDGQTVAVGDVLFVIDPAPYEAAMAKAEASLTSARTNAAFARTELNRAVSLVKTQTIAQRLYDERANAN
RLAQAAVLVAEAEVKRARIDLDHTRVKAPIAGRVSRAEITVGNVVQPGAGAPLLTSIVSNDGLYADFEVDEQTYMSGIRA
RGPSRDQENRIPVQITAQGDEAHPYGGTIHSFDNRIDSATGTIRARALLANEDGALMPGMFVSVKVASGGGAGALMVQER
AIGNDQSKKFVYVVGEDAKVAYREVDLGARVEGERIVLSGLRAGERVIVDGLQQVRPTMTVEIKEAGLETASRALAAK

Sequences:

>Translated_398_residues
MTKGSRFVKGRAVLLGTVGLILLAGGVGVVLDRGVGVPAQAQAPAQAPRVGVQTLTPRQVQVWSSFSGRLRAVDLAEIRP
EVGGRLTEVRFRDGQTVAVGDVLFVIDPAPYEAAMAKAEASLTSARTNAAFARTELNRAVSLVKTQTIAQRLYDERANAN
RLAQAAVLVAEAEVKRARIDLDHTRVKAPIAGRVSRAEITVGNVVQPGAGAPLLTSIVSNDGLYADFEVDEQTYMSGIRA
RGPSRDQENRIPVQITAQGDEAHPYGGTIHSFDNRIDSATGTIRARALLANEDGALMPGMFVSVKVASGGGAGALMVQER
AIGNDQSKKFVYVVGEDAKVAYREVDLGARVEGERIVLSGLRAGERVIVDGLQQVRPTMTVEIKEAGLETASRALAAK
>Mature_397_residues
TKGSRFVKGRAVLLGTVGLILLAGGVGVVLDRGVGVPAQAQAPAQAPRVGVQTLTPRQVQVWSSFSGRLRAVDLAEIRPE
VGGRLTEVRFRDGQTVAVGDVLFVIDPAPYEAAMAKAEASLTSARTNAAFARTELNRAVSLVKTQTIAQRLYDERANANR
LAQAAVLVAEAEVKRARIDLDHTRVKAPIAGRVSRAEITVGNVVQPGAGAPLLTSIVSNDGLYADFEVDEQTYMSGIRAR
GPSRDQENRIPVQITAQGDEAHPYGGTIHSFDNRIDSATGTIRARALLANEDGALMPGMFVSVKVASGGGAGALMVQERA
IGNDQSKKFVYVVGEDAKVAYREVDLGARVEGERIVLSGLRAGERVIVDGLQQVRPTMTVEIKEAGLETASRALAAK

Specific function: Part of a multidrug resistance efflux system that confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=353, Percent_Identity=35.4107648725212, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1786668, Length=352, Percent_Identity=32.3863636363636, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1789665, Length=380, Percent_Identity=30.7894736842105, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI87082036, Length=321, Percent_Identity=28.9719626168224, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI87081951, Length=179, Percent_Identity=32.4022346368715, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081796, Length=342, Percent_Identity=24.2690058479532, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790519, Length=224, Percent_Identity=29.0178571428571, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789637, Length=166, Percent_Identity=27.710843373494, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42168; Mature: 42036

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKGSRFVKGRAVLLGTVGLILLAGGVGVVLDRGVGVPAQAQAPAQAPRVGVQTLTPRQV
CCCCCCEECCCEEEHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCE
QVWSSFSGRLRAVDLAEIRPEVGGRLTEVRFRDGQTVAVGDVLFVIDPAPYEAAMAKAEA
EEECCCCCCEEEEEHHHHCHHHCCEEEEEEECCCCEEEEEEEEEEECCCCHHHHHHHHHH
SLTSARTNAAFARTELNRAVSLVKTQTIAQRLYDERANANRLAQAAVLVAEAEVKRARID
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEC
LDHTRVKAPIAGRVSRAEITVGNVVQPGAGAPLLTSIVSNDGLYADFEVDEQTYMSGIRA
CCHHEEECCCCCCCEEEEEEECCEECCCCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHC
RGPSRDQENRIPVQITAQGDEAHPYGGTIHSFDNRIDSATGTIRARALLANEDGALMPGM
CCCCCCCCCCCCEEEEECCCCCCCCCCEEHHHHHHHCCCCCCEEEEEEEECCCCCCCCCE
FVSVKVASGGGAGALMVQERAIGNDQSKKFVYVVGEDAKVAYREVDLGARVEGERIVLSG
EEEEEEECCCCCEEEEEEEHHCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCEEEEEC
LRAGERVIVDGLQQVRPTMTVEIKEAGLETASRALAAK
CCCCCHHHHHHHHHCCCEEEEEEHHHCHHHHHHHHCCC
>Mature Secondary Structure 
TKGSRFVKGRAVLLGTVGLILLAGGVGVVLDRGVGVPAQAQAPAQAPRVGVQTLTPRQV
CCCCCEECCCEEEHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCE
QVWSSFSGRLRAVDLAEIRPEVGGRLTEVRFRDGQTVAVGDVLFVIDPAPYEAAMAKAEA
EEECCCCCCEEEEEHHHHCHHHCCEEEEEEECCCCEEEEEEEEEEECCCCHHHHHHHHHH
SLTSARTNAAFARTELNRAVSLVKTQTIAQRLYDERANANRLAQAAVLVAEAEVKRARID
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEC
LDHTRVKAPIAGRVSRAEITVGNVVQPGAGAPLLTSIVSNDGLYADFEVDEQTYMSGIRA
CCHHEEECCCCCCCEEEEEEECCEECCCCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHC
RGPSRDQENRIPVQITAQGDEAHPYGGTIHSFDNRIDSATGTIRARALLANEDGALMPGM
CCCCCCCCCCCCEEEEECCCCCCCCCCEEHHHHHHHCCCCCCEEEEEEEECCCCCCCCCE
FVSVKVASGGGAGALMVQERAIGNDQSKKFVYVVGEDAKVAYREVDLGARVEGERIVLSG
EEEEEEECCCCCEEEEEEEHHCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCEEEEEC
LRAGERVIVDGLQQVRPTMTVEIKEAGLETASRALAAK
CCCCCHHHHHHHHHCCCEEEEEEHHHCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]