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The map label for this gene is phoB [H]

Identifier: 83591942

GI number: 83591942

Start: 716384

End: 717085

Strand: Direct

Name: phoB [H]

Synonym: Rru_A0603

Alternate gene names: 83591942

Gene position: 716384-717085 (Clockwise)

Preceding gene: 83591941

Following gene: 83591943

Centisome position: 16.46

GC content: 63.11

Gene sequence:

>702_bases
ATGAAATCCCATGTGCTGATCGTTGAAGACGAAGTCTCGCTGGTGACCTTGCTGCGCTATAACCTTGAGAAAGAGGGTTA
CCGGGTGTCCGAGGCGATGGATGGCGAGGAAGCCATGACCCTGGTCGCCGAGGATCAGCCCGATGTGGTGATCCTTGACT
GGATGCTGCCGTCGCTGTCGGGGATCGAGGTCTGCCGCCAGCTGCGGCGCAAGCCGCGCACCCGCGATCTGCCGATTTTG
ATGCTGACGGCGCGCGGCGAGGAAAGTGACCGCATTCGCGGCCTCAATACCGGGGCCGACGACTATATGTCCAAGCCGTT
TTCCATGCCCGAACTGCTGGCCCGGGTCAAAGCGCTGCTGCGCCGCGCCCAGCCGACCAGCGCCAAGGGCACGCTGAGCT
ATTCCGATGTGGTGATGGATCTGGGCGCCCATCGGGTGACCCGGGGGGGACGCTATATCCACCTCGGGCCGACGGAGTTC
CGTCTGCTGCAGTTCCTGATGCAGAACCCGGGCGCGGTGTTTTCCCGCGAAGAACTGCTGAACGCCGTTTGGGGACCCGA
CATCTACGTCGAACCCCGCACCGTTGATGTTCATATCCGCCGTCTGCGCAAGGCGCTCAACGGCGAGGCCAGCACCGATC
TGATCCGCACCGTCCGTGCCGCCGGCTACGCGCTTGATACCAACCCCGTCTTCTCCCAATAG

Upstream 100 bases:

>100_bases
ATTACCTGGTTTCGGGAAATCCGCTACGCGCCGCCCGCCCCAAAGGAAGCGGTCAGGGCGAGTTCGGGGACACCCTTGAT
GACATTCAAGAGCTGAACCG

Downstream 100 bases:

>100_bases
GAGGGTCCGCGCCATGTCCCATGTCACCGTCTTGTGGAGCAGCCCCGATCTGGCAACCCTGGAGGCGGTCCAGGCCGTGG
CCGGTCCCGTCAAGGCGCCG

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MKSHVLIVEDEVSLVTLLRYNLEKEGYRVSEAMDGEEAMTLVAEDQPDVVILDWMLPSLSGIEVCRQLRRKPRTRDLPIL
MLTARGEESDRIRGLNTGADDYMSKPFSMPELLARVKALLRRAQPTSAKGTLSYSDVVMDLGAHRVTRGGRYIHLGPTEF
RLLQFLMQNPGAVFSREELLNAVWGPDIYVEPRTVDVHIRRLRKALNGEASTDLIRTVRAAGYALDTNPVFSQ

Sequences:

>Translated_233_residues
MKSHVLIVEDEVSLVTLLRYNLEKEGYRVSEAMDGEEAMTLVAEDQPDVVILDWMLPSLSGIEVCRQLRRKPRTRDLPIL
MLTARGEESDRIRGLNTGADDYMSKPFSMPELLARVKALLRRAQPTSAKGTLSYSDVVMDLGAHRVTRGGRYIHLGPTEF
RLLQFLMQNPGAVFSREELLNAVWGPDIYVEPRTVDVHIRRLRKALNGEASTDLIRTVRAAGYALDTNPVFSQ
>Mature_233_residues
MKSHVLIVEDEVSLVTLLRYNLEKEGYRVSEAMDGEEAMTLVAEDQPDVVILDWMLPSLSGIEVCRQLRRKPRTRDLPIL
MLTARGEESDRIRGLNTGADDYMSKPFSMPELLARVKALLRRAQPTSAKGTLSYSDVVMDLGAHRVTRGGRYIHLGPTEF
RLLQFLMQNPGAVFSREELLNAVWGPDIYVEPRTVDVHIRRLRKALNGEASTDLIRTVRAAGYALDTNPVFSQ

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=227, Percent_Identity=48.8986784140969, Blast_Score=234, Evalue=4e-63,
Organism=Escherichia coli, GI1786911, Length=205, Percent_Identity=43.4146341463415, Blast_Score=159, Evalue=1e-40,
Organism=Escherichia coli, GI1788394, Length=221, Percent_Identity=41.1764705882353, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1786784, Length=223, Percent_Identity=37.6681614349776, Blast_Score=150, Evalue=7e-38,
Organism=Escherichia coli, GI87082012, Length=221, Percent_Identity=37.10407239819, Blast_Score=142, Evalue=1e-35,
Organism=Escherichia coli, GI1790860, Length=228, Percent_Identity=39.4736842105263, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1789809, Length=228, Percent_Identity=34.6491228070175, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1789402, Length=221, Percent_Identity=35.7466063348416, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI2367329, Length=231, Percent_Identity=37.2294372294372, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1790863, Length=232, Percent_Identity=36.2068965517241, Blast_Score=127, Evalue=4e-31,
Organism=Escherichia coli, GI1787229, Length=236, Percent_Identity=36.0169491525424, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1790552, Length=224, Percent_Identity=37.0535714285714, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=34.0807174887892, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI145693140, Length=233, Percent_Identity=30.9012875536481, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI48994928, Length=116, Percent_Identity=37.0689655172414, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788550, Length=111, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1788191, Length=118, Percent_Identity=28.8135593220339, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790437, Length=125, Percent_Identity=29.6, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26281; Mature: 26281

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSHVLIVEDEVSLVTLLRYNLEKEGYRVSEAMDGEEAMTLVAEDQPDVVILDWMLPSLS
CCCCEEEEECCHHHHHHHHHHHCCCCEEHHHHCCCHHHEEEEECCCCCEEEEECCCCCCC
GIEVCRQLRRKPRTRDLPILMLTARGEESDRIRGLNTGADDYMSKPFSMPELLARVKALL
HHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEECCCCCHHHHHCCCCCHHHHHHHHHHHH
RRAQPTSAKGTLSYSDVVMDLGAHRVTRGGRYIHLGPTEFRLLQFLMQNPGAVFSREELL
HHCCCCCCCCCCCHHHHHHHHCCHHHHCCCCEEEECCHHHHHHHHHHCCCCCCCCHHHHH
NAVWGPDIYVEPRTVDVHIRRLRKALNGEASTDLIRTVRAAGYALDTNPVFSQ
HHHCCCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCEEECCCCCCCC
>Mature Secondary Structure
MKSHVLIVEDEVSLVTLLRYNLEKEGYRVSEAMDGEEAMTLVAEDQPDVVILDWMLPSLS
CCCCEEEEECCHHHHHHHHHHHCCCCEEHHHHCCCHHHEEEEECCCCCEEEEECCCCCCC
GIEVCRQLRRKPRTRDLPILMLTARGEESDRIRGLNTGADDYMSKPFSMPELLARVKALL
HHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEECCCCCHHHHHCCCCCHHHHHHHHHHHH
RRAQPTSAKGTLSYSDVVMDLGAHRVTRGGRYIHLGPTEFRLLQFLMQNPGAVFSREELL
HHCCCCCCCCCCCHHHHHHHHCCHHHHCCCCEEEECCHHHHHHHHHHCCCCCCCCHHHHH
NAVWGPDIYVEPRTVDVHIRRLRKALNGEASTDLIRTVRAAGYALDTNPVFSQ
HHHCCCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]