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The map label for this gene is btuB [H]

Identifier: 83591828

GI number: 83591828

Start: 573218

End: 575266

Strand: Direct

Name: btuB [H]

Synonym: Rru_A0488

Alternate gene names: 83591828

Gene position: 573218-575266 (Clockwise)

Preceding gene: 83591827

Following gene: 83591829

Centisome position: 13.17

GC content: 65.98

Gene sequence:

>2049_bases
ATGGTCATCGGCTGCGCGGCGTTCCCGGATCGGGAAGCCGCGTTGGGGGGACGTGTCATGGGTTATGGATCGCCTGATCG
CGCGTGGCGCGCGGGGATGGGAGTGTTTGGGGTGGCCGTCGCCGCCGTGATGGCGGGGGTGGTCGGCGCCAGCGGGGCCG
CCGCTTGGGCCGACGAGCCGGAAACCACCGAAGGGCTGATCGTTTCGGCCAGCCGGACCCCGGTGGCGGCGAAGGATCTC
GGGCGCTCGGTCAGCGTCCTTACCGGCGAGGACTTGCGCGAACGCGGCGTGCGCTATGTGTCCGACGCCCTGCGCCAATT
GCCCGGCGTCGCCGTCAGTCGGGCCGGCGGGCCCGGCGGCGTGACCCAGGTGCGCCTTCGCGGCGCCGAGGCCAACCATG
TTCTGGTGCTGATCGATGGCGTGGCCGTATCGTCGCCGGCTTCGGGCGAATACGATTTCGCCGGTCTGGTGGCCAGCGAT
ATCGAGCGCATCGAGGTGCTGCGCGGGCCGCAATCAGCACTTTGGGGCTCCAACGCCACCGCCGGCGTCATCAGCATCGT
TACCCGGCGCGGCACGCGCAACCAGAGCGGCGGCGAGGTGAGCCTGGACGGCGGCAGCGATGGCACCTTCGGCGGATCGG
TCGCCATGGGGGCGGGCACCGAGGATCTCGACGCCGCCCTGTCGCTGGCGGGCTTGCGGGCCGGCGGGCTGGATGTCTCG
GCCGATCCCGGCGGCGATATCGACCGCGACCGCAACATCACCCTGAACGGCAAGATCAATTACGACGCCAGCGACTGGCT
GCGCTTTGGCGCCACGGCGCGGATGGCCGACCGCCGCTCCGATTACGACGGCTTCGCCTTTGGCGCCCCCAAGCGCTCCG
ATCTGGTCTATGACGGGGATTATGCCACCGATCAGCGCGATGTCGCGGTATCGGGCCATGTCGATGTCGATAGCCTGGGC
GGCAGGCTGAGCCATCAGGCGCGTTTTGACTATACGCGGGTCGATATCCAGACCCTGCTTGATGGGCTGTCCACCGCCAA
ATCCCTGGGGCAGCGCTTCAAGGGGACTTATCAGACGACGCTGGCGCTTGATGCGCCGACGCTAACCAGCGCCAATCACC
GGCTGACGGCGGCGGTGGATTGGCAGCGCGAGACTTTCCGCAACACCGATGCCGATCTGGTTTCCGATCCCTCGCAGGTG
GAAGGCCAGCAGCGCGATCTTTACGGGCTGGTCGCCGAATATCGCGGCACGCTGTTTCAAGATCTTGATCTGCAGGCAGC
CCTCCGTCACGACATCAACGACAGCTTCGCCGATTCGACGACCTTTAGCACCAGCGCGTCCTATGAGGTGCGGCCAACCC
AGTCGCGTCTGCACGCCTCGGTTGGCACCGGCGTGACCAATCCGACCTTTTATGAGCAATTCGGCTTCATCCCCAGCCAG
TTCATCGGCAACGCCAACCTCGATCCCGAGAAGAACTTCGGCTGGGACGCCGGCATCGAGCAGCGCATCCTCGGCGATCA
ATTGACCCTGGACGCCACTTATTTCCATGAAACCCTGACCGATGAGATCGTCACCGTCTATAAGGCGCCCAGCTACCTGG
GGACGGCGGTCAACGAGAATGGCGACAGCCGCCGCCAGGGGATCGAACTGACGCTAACGGCGCGGCCGACCGAGGCTTTG
ACCCTGACGGGCAATTACACCTGGACCGACGCCTATGATCCCGACGACAAGATCGAGGTCCGCCGTCCGCGCCACCGCGC
CACTCTGGCCGCCGACTACCGCTTCCTTGACAACCGCGCCGGCGTGAACGCCGAGGTGGTCTATGCGGCGGGCAATTACG
ATTTCGATTACCGGGCGGCGAGCATGGGGAGCGCCCGGCTGCGGCTTGATGATTATGCCCTGCTCAATCTGGGGGGACGC
TACAAGGTGACCGAGGCCGTCGAGGTCTATGGCCGGATCGAGAACGCCCTTGATACCCAATATGCCGATCTGGACGGCTA
CGCCACCCAGGGCGTGACCGGCTATTTCGGCCTGCGGGCCCGGTTCTAG

Upstream 100 bases:

>100_bases
GCTGGTCGTCACCTAACCCTGTGCGCGGGGCGCGCCATGGGGCGGTTTTTCTCCGCAATGGTGGCACATATCCGCTGGTC
TGCCCGGATTCGGGACGGCC

Downstream 100 bases:

>100_bases
GGGGCATCGGGGTGATCGGCCGTCCTCATCGGCTGCTGCGGCGGCTTCTGGGGGGGGCGGCGGTGATCGCCGCCCTTCTT
GGTCCGCCGGCGGCGCTGGC

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 682; Mature: 682

Protein sequence:

>682_residues
MVIGCAAFPDREAALGGRVMGYGSPDRAWRAGMGVFGVAVAAVMAGVVGASGAAAWADEPETTEGLIVSASRTPVAAKDL
GRSVSVLTGEDLRERGVRYVSDALRQLPGVAVSRAGGPGGVTQVRLRGAEANHVLVLIDGVAVSSPASGEYDFAGLVASD
IERIEVLRGPQSALWGSNATAGVISIVTRRGTRNQSGGEVSLDGGSDGTFGGSVAMGAGTEDLDAALSLAGLRAGGLDVS
ADPGGDIDRDRNITLNGKINYDASDWLRFGATARMADRRSDYDGFAFGAPKRSDLVYDGDYATDQRDVAVSGHVDVDSLG
GRLSHQARFDYTRVDIQTLLDGLSTAKSLGQRFKGTYQTTLALDAPTLTSANHRLTAAVDWQRETFRNTDADLVSDPSQV
EGQQRDLYGLVAEYRGTLFQDLDLQAALRHDINDSFADSTTFSTSASYEVRPTQSRLHASVGTGVTNPTFYEQFGFIPSQ
FIGNANLDPEKNFGWDAGIEQRILGDQLTLDATYFHETLTDEIVTVYKAPSYLGTAVNENGDSRRQGIELTLTARPTEAL
TLTGNYTWTDAYDPDDKIEVRRPRHRATLAADYRFLDNRAGVNAEVVYAAGNYDFDYRAASMGSARLRLDDYALLNLGGR
YKVTEAVEVYGRIENALDTQYADLDGYATQGVTGYFGLRARF

Sequences:

>Translated_682_residues
MVIGCAAFPDREAALGGRVMGYGSPDRAWRAGMGVFGVAVAAVMAGVVGASGAAAWADEPETTEGLIVSASRTPVAAKDL
GRSVSVLTGEDLRERGVRYVSDALRQLPGVAVSRAGGPGGVTQVRLRGAEANHVLVLIDGVAVSSPASGEYDFAGLVASD
IERIEVLRGPQSALWGSNATAGVISIVTRRGTRNQSGGEVSLDGGSDGTFGGSVAMGAGTEDLDAALSLAGLRAGGLDVS
ADPGGDIDRDRNITLNGKINYDASDWLRFGATARMADRRSDYDGFAFGAPKRSDLVYDGDYATDQRDVAVSGHVDVDSLG
GRLSHQARFDYTRVDIQTLLDGLSTAKSLGQRFKGTYQTTLALDAPTLTSANHRLTAAVDWQRETFRNTDADLVSDPSQV
EGQQRDLYGLVAEYRGTLFQDLDLQAALRHDINDSFADSTTFSTSASYEVRPTQSRLHASVGTGVTNPTFYEQFGFIPSQ
FIGNANLDPEKNFGWDAGIEQRILGDQLTLDATYFHETLTDEIVTVYKAPSYLGTAVNENGDSRRQGIELTLTARPTEAL
TLTGNYTWTDAYDPDDKIEVRRPRHRATLAADYRFLDNRAGVNAEVVYAAGNYDFDYRAASMGSARLRLDDYALLNLGGR
YKVTEAVEVYGRIENALDTQYADLDGYATQGVTGYFGLRARF
>Mature_682_residues
MVIGCAAFPDREAALGGRVMGYGSPDRAWRAGMGVFGVAVAAVMAGVVGASGAAAWADEPETTEGLIVSASRTPVAAKDL
GRSVSVLTGEDLRERGVRYVSDALRQLPGVAVSRAGGPGGVTQVRLRGAEANHVLVLIDGVAVSSPASGEYDFAGLVASD
IERIEVLRGPQSALWGSNATAGVISIVTRRGTRNQSGGEVSLDGGSDGTFGGSVAMGAGTEDLDAALSLAGLRAGGLDVS
ADPGGDIDRDRNITLNGKINYDASDWLRFGATARMADRRSDYDGFAFGAPKRSDLVYDGDYATDQRDVAVSGHVDVDSLG
GRLSHQARFDYTRVDIQTLLDGLSTAKSLGQRFKGTYQTTLALDAPTLTSANHRLTAAVDWQRETFRNTDADLVSDPSQV
EGQQRDLYGLVAEYRGTLFQDLDLQAALRHDINDSFADSTTFSTSASYEVRPTQSRLHASVGTGVTNPTFYEQFGFIPSQ
FIGNANLDPEKNFGWDAGIEQRILGDQLTLDATYFHETLTDEIVTVYKAPSYLGTAVNENGDSRRQGIELTLTARPTEAL
TLTGNYTWTDAYDPDDKIEVRRPRHRATLAADYRFLDNRAGVNAEVVYAAGNYDFDYRAASMGSARLRLDDYALLNLGGR
YKVTEAVEVYGRIENALDTQYADLDGYATQGVTGYFGLRARF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=656, Percent_Identity=28.0487804878049, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1788478, Length=700, Percent_Identity=23.5714285714286, Blast_Score=106, Evalue=5e-24,
Organism=Escherichia coli, GI1786798, Length=165, Percent_Identity=35.1515151515151, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787723, Length=712, Percent_Identity=24.0168539325843, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 73061; Mature: 73061

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIGCAAFPDREAALGGRVMGYGSPDRAWRAGMGVFGVAVAAVMAGVVGASGAAAWADEP
CEEEECCCCCCCHHCCCEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
ETTEGLIVSASRTPVAAKDLGRSVSVLTGEDLRERGVRYVSDALRQLPGVAVSRAGGPGG
CCCCCEEEECCCCCCHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCEEECCCCCCC
VTQVRLRGAEANHVLVLIDGVAVSSPASGEYDFAGLVASDIERIEVLRGPQSALWGSNAT
CEEEEEECCCCCEEEEEEECEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCC
AGVISIVTRRGTRNQSGGEVSLDGGSDGTFGGSVAMGAGTEDLDAALSLAGLRAGGLDVS
HHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEC
ADPGGDIDRDRNITLNGKINYDASDWLRFGATARMADRRSDYDGFAFGAPKRSDLVYDGD
CCCCCCCCCCCEEEEEEEECCCHHHHHHHCCHHHHHHCCCCCCCEEECCCCCCCEEECCC
YATDQRDVAVSGHVDVDSLGGRLSHQARFDYTRVDIQTLLDGLSTAKSLGQRFKGTYQTT
CCCCCCCEEEECCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
LALDAPTLTSANHRLTAAVDWQRETFRNTDADLVSDPSQVEGQQRDLYGLVAEYRGTLFQ
EEECCCCCCCCCCEEEEEECCCHHHHCCCCCHHHCCCHHHCCCHHHHHHHHHHHHCCHHH
DLDLQAALRHDINDSFADSTTFSTSASYEVRPTQSRLHASVGTGVTNPTFYEQFGFIPSQ
CCCHHHHHHHCCCCCCCCCCEECCCCCEEECCCHHHHHHHHCCCCCCCHHHHHHCCCHHH
FIGNANLDPEKNFGWDAGIEQRILGDQLTLDATYFHETLTDEIVTVYKAPSYLGTAVNEN
HCCCCCCCCCCCCCCCCCCHHHHCCCEEEEEHHHHHHHHHHHHHEEECCCCHHHHHHCCC
GDSRRQGIELTLTARPTEALTLTGNYTWTDAYDPDDKIEVRRPRHRATLAADYRFLDNRA
CCCHHCCEEEEEEECCCEEEEEECCEEECCCCCCCCCEEECCCCHHHEEEEHHHHHHCCC
GVNAEVVYAAGNYDFDYRAASMGSARLRLDDYALLNLGGRYKVTEAVEVYGRIENALDTQ
CCCEEEEEEECCCCCCEEECCCCCEEEEECCEEEEECCCCEEHHHHHHHHHHHHHHHCCC
YADLDGYATQGVTGYFGLRARF
CCCCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MVIGCAAFPDREAALGGRVMGYGSPDRAWRAGMGVFGVAVAAVMAGVVGASGAAAWADEP
CEEEECCCCCCCHHCCCEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
ETTEGLIVSASRTPVAAKDLGRSVSVLTGEDLRERGVRYVSDALRQLPGVAVSRAGGPGG
CCCCCEEEECCCCCCHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCEEECCCCCCC
VTQVRLRGAEANHVLVLIDGVAVSSPASGEYDFAGLVASDIERIEVLRGPQSALWGSNAT
CEEEEEECCCCCEEEEEEECEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCC
AGVISIVTRRGTRNQSGGEVSLDGGSDGTFGGSVAMGAGTEDLDAALSLAGLRAGGLDVS
HHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEC
ADPGGDIDRDRNITLNGKINYDASDWLRFGATARMADRRSDYDGFAFGAPKRSDLVYDGD
CCCCCCCCCCCEEEEEEEECCCHHHHHHHCCHHHHHHCCCCCCCEEECCCCCCCEEECCC
YATDQRDVAVSGHVDVDSLGGRLSHQARFDYTRVDIQTLLDGLSTAKSLGQRFKGTYQTT
CCCCCCCEEEECCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
LALDAPTLTSANHRLTAAVDWQRETFRNTDADLVSDPSQVEGQQRDLYGLVAEYRGTLFQ
EEECCCCCCCCCCEEEEEECCCHHHHCCCCCHHHCCCHHHCCCHHHHHHHHHHHHCCHHH
DLDLQAALRHDINDSFADSTTFSTSASYEVRPTQSRLHASVGTGVTNPTFYEQFGFIPSQ
CCCHHHHHHHCCCCCCCCCCEECCCCCEEECCCHHHHHHHHCCCCCCCHHHHHHCCCHHH
FIGNANLDPEKNFGWDAGIEQRILGDQLTLDATYFHETLTDEIVTVYKAPSYLGTAVNEN
HCCCCCCCCCCCCCCCCCCHHHHCCCEEEEEHHHHHHHHHHHHHEEECCCCHHHHHHCCC
GDSRRQGIELTLTARPTEALTLTGNYTWTDAYDPDDKIEVRRPRHRATLAADYRFLDNRA
CCCHHCCEEEEEEECCCEEEEEECCEEECCCCCCCCCEEECCCCHHHEEEEHHHHHHCCC
GVNAEVVYAAGNYDFDYRAASMGSARLRLDDYALLNLGGRYKVTEAVEVYGRIENALDTQ
CCCEEEEEEECCCCCCEEECCCCCEEEEECCEEEEECCCCEEHHHHHHHHHHHHHHHCCC
YADLDGYATQGVTGYFGLRARF
CCCCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA