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The map label for this gene is rhtB [H]

Identifier: 83591668

GI number: 83591668

Start: 400635

End: 401267

Strand: Direct

Name: rhtB [H]

Synonym: Rru_A0328

Alternate gene names: 83591668

Gene position: 400635-401267 (Clockwise)

Preceding gene: 83591667

Following gene: 83591669

Centisome position: 9.2

GC content: 62.56

Gene sequence:

>633_bases
ATGACGTTTCACGAAATCTTGCTGTTCATCCCCGTCGGCCTGTTGATGGCCGTGCCCTTTGGTCCGAGCAACTTTTTGGT
TTTTTCCAATGGTCTGCGCTATGGGCCGGTGCCCGCCGGTTTGGCCGGGGTGGCGCGCATCGTCGCCTTTGGGCTTTTGA
TCGGCATTACCGCTTTCGGTCTGGGGGCGATGCTGTCTTCGGCGGCGTGGTTGCTGATCGGCATCAAGTGGTTCGGCATC
GCCTATCTGGCGTGGCTTGGCATCCGCATCTGGCGCACGCCGGCGCCCGATGTCGAGGCCTTGCGCTTGGTGGCGGGAGC
CGCGCGGCCGCAAGGAACCTTGCGGGCGCTGCTGCGCATGGAATTCACCACCGCCATCGCCAATCCCAAGGCGATCTTGT
ATTTTACCGCCGCCTTTCCCCAGTTCATCGGCCCGGCCGAGAACTTCACCGAACGCTTCCTGACCATGGGCGCGGTGTTC
CTGGTCTGTGAATACATCGTGCTGTGGGGCTATGGGCTGGTTGGCGCCGGGCTTGGGCGTACGGGCCTGCTTGCCGGGAT
GAGGGATTGGATCAACAAGATCACCGGCGCCAGCTTCCTTGGCTTCGCCGCCTGGATGGCCAGCGCCAAATAG

Upstream 100 bases:

>100_bases
GGGAGCCGATTTCACGCCAGCGGCGTGAGGGATTGAGAACGGGCGTGGTTGCCCCCTGGGCGGGCGGGATCTAAGGTGGG
GCGCGCTTTGGAGGTCGGCC

Downstream 100 bases:

>100_bases
CGCCGTAACACGACGCGGCATAAAAGAGTACGGGGCGCGGTCCGACATGGCTCGCGCCGGCCGTCTGCAAGACGGCATAA
AAAACTACGGGGCGCGGTCG

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MTFHEILLFIPVGLLMAVPFGPSNFLVFSNGLRYGPVPAGLAGVARIVAFGLLIGITAFGLGAMLSSAAWLLIGIKWFGI
AYLAWLGIRIWRTPAPDVEALRLVAGAARPQGTLRALLRMEFTTAIANPKAILYFTAAFPQFIGPAENFTERFLTMGAVF
LVCEYIVLWGYGLVGAGLGRTGLLAGMRDWINKITGASFLGFAAWMASAK

Sequences:

>Translated_210_residues
MTFHEILLFIPVGLLMAVPFGPSNFLVFSNGLRYGPVPAGLAGVARIVAFGLLIGITAFGLGAMLSSAAWLLIGIKWFGI
AYLAWLGIRIWRTPAPDVEALRLVAGAARPQGTLRALLRMEFTTAIANPKAILYFTAAFPQFIGPAENFTERFLTMGAVF
LVCEYIVLWGYGLVGAGLGRTGLLAGMRDWINKITGASFLGFAAWMASAK
>Mature_209_residues
TFHEILLFIPVGLLMAVPFGPSNFLVFSNGLRYGPVPAGLAGVARIVAFGLLIGITAFGLGAMLSSAAWLLIGIKWFGIA
YLAWLGIRIWRTPAPDVEALRLVAGAARPQGTLRALLRMEFTTAIANPKAILYFTAAFPQFIGPAENFTERFLTMGAVFL
VCEYIVLWGYGLVGAGLGRTGLLAGMRDWINKITGASFLGFAAWMASAK

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=195, Percent_Identity=31.7948717948718, Blast_Score=70, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22532; Mature: 22401

Theoretical pI: Translated: 10.50; Mature: 10.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFHEILLFIPVGLLMAVPFGPSNFLVFSNGLRYGPVPAGLAGVARIVAFGLLIGITAFG
CCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LGAMLSSAAWLLIGIKWFGIAYLAWLGIRIWRTPAPDVEALRLVAGAARPQGTLRALLRM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHH
EFTTAIANPKAILYFTAAFPQFIGPAENFTERFLTMGAVFLVCEYIVLWGYGLVGAGLGR
HHHHHHCCCCEEEEEHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
TGLLAGMRDWINKITGASFLGFAAWMASAK
HHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TFHEILLFIPVGLLMAVPFGPSNFLVFSNGLRYGPVPAGLAGVARIVAFGLLIGITAFG
CHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LGAMLSSAAWLLIGIKWFGIAYLAWLGIRIWRTPAPDVEALRLVAGAARPQGTLRALLRM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHH
EFTTAIANPKAILYFTAAFPQFIGPAENFTERFLTMGAVFLVCEYIVLWGYGLVGAGLGR
HHHHHHCCCCEEEEEHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
TGLLAGMRDWINKITGASFLGFAAWMASAK
HHHHHHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]