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The map label for this gene is asnB [H]

Identifier: 83591570

GI number: 83591570

Start: 282580

End: 284556

Strand: Direct

Name: asnB [H]

Synonym: Rru_A0230

Alternate gene names: 83591570

Gene position: 282580-284556 (Clockwise)

Preceding gene: 83591563

Following gene: 161777629

Centisome position: 6.49

GC content: 70.41

Gene sequence:

>1977_bases
ATGTGCGGGCTGACGGGCTATTGGACGCGGGGCGGTGACGGGCGCGGCCGGGGCGAAATGACCGCGCTGGTGCTGCGCAT
GGCCGGGGCCCTGGCCCATCGCGGACCCGATGGCGATGGCGCCTGGGTCGATGAGGACGCCGGCTTGGCCTTGGGCCATC
GGCGGCTGTCGATCGTCGATCTGTCGCCCGCCGGCCAGCAGCCGATGGTCTCGGCCGATGGCCGCCTCGCCCTGGCCTAT
AACGGCGAGATCTATAACGCGGCCGAGCTGCGCCCCGAGCTTGAAGCCGCCGGTATCGTCTTTCGCGGCCATTCCGACAC
CGAGGTTCTGCTGGAAGGCTTCGCCCGCTGGGGGGTGGTGCCCACCTTGCGGCGCGCCATCGGCATGTTCGCCTTCGCCC
TGTGGGACCGCAAGGAGCGCCGGCTGACCCTAGGGCGCGACCGTTTGGGCATCAAGCCGCTTTACGTCGCCGACCATGGC
GGCACGCTGCTGTTTGCCTCGCAGCCCAAGGGGATGATGCCCCATCCCTCCTGGCGGGGCGAGGTCGACCGCGAAGGGCT
GGCGGCGATGCTGCGGCTGTCGTGCCTGCCGGCGCCGCGCTCGATCTTCAAGGGGGTGAGCCAACTGGCGCCGGGAACGG
TGGTGCGCTTCGAAGCCGATGGCCGGCGCTCGGAAGAGGTGTTCTGGTCGCTTGAGGAGGTCGCCGCCCGTGGCGTCGCC
CAGGCCCGATCCCAGCCGGCCCGCCCCGAAAGCGCTTCCCTGGCGGTCGAGCACCTGGAAAGCCTGCTGCTTGATGCCGT
GCGCCGGCGGTTGATCGCCGATGTGCCCGTCGGCGCCTTCCTGTCGGGGGGGGTGGACAGCTCCACCGTCGTCGCCCTGA
TGCGCAAGGTGGCCAGCGGCCCGGTTAAGACCTTCTCGATCGGCTTCGCCGAGGAGGGGTGGGACGAATCCGTCCATGCC
GCCGCCGTCGCCCGCCATCTGGGAACCGATCACCGCGAACTGGTGGTCTCGCCCGACGAGGTGCCGGCGATTGTGGGCGA
TTTGCCGCGCTGGTATGACGAACCCTTCGCCGACGCCTCGCAGATCCCCACCCTGCTGGTCTCGAAACTGGCGCGATCCG
AGGTCACGGTCAGCCTGTCGGGCGATGGCGGCGACGAGCTGTTCGCCGGCTATAACCGCCATGTTCAGGGCCAGCGGCTA
TGGGACTTGGCGCAACGGATCCCGACCGTCGGCCGCGATCTCGGCGCCGCGCTGATCGAGGCGCCGTCGCCGGGTTTTTG
GCAGCGGGCGGTGGGTCTGCTGCCCGCCGGCCGCCGGCCGCCCCAGGCCGCCGACAAGCCGGCCAAGATCGCCCGCCTGC
TGCGCGCCCGCAGCCCCGAGGCGCTCTATCGCACCCTGGTCGGCGCCATGCCGTCCGCCGAGGTGCCCTTGGCCGGGGTG
TCGCTGGCCGCCGAGGAGCGTTGGTACGCCGCCTCCGCCGAGGGGGTCGATAGCTTCGTTGACCGCATGCGCCTGCTCGA
CGCCAAGGGCTATCTACCCGACGACATCCTGACCAAGGTCGACCGGGCGTCGATGGCCTATGGGCTGGAGGCGCGGGTGC
CGCTGCTTGATCACCGGGTGGTGGAATTCGCCTGGAGTCTGCCGCCCGAGATGCTGATCCGCGAGGGCCAGGGCAAATGG
CTGCTGCGCCGGGTGCTCCATCGCCATGTGCCGCGCGCCCTGATCGAACGGCCGAAGATGGGCTTTGGCGTGCCGCTGGC
CGCCTGGTTGCGCGGCCCCTTGCGTGGGTGGGCCGAGGATTTGCTTGATCCGCAGACGATGGCCGTTCAAGGGTTGATCG
ATCCGGCGCCGGTGCGCCGCTGGTGGCTTGATCATCTGGCCGGGCGGCGCGACACCCACGACCGGCTGTGGCCGGTGCTT
TCGGCGCTTTCCTGGCATCACCACTGGAAAAGCGCCGGCGCCTTCACCGCCGCCTGA

Upstream 100 bases:

>100_bases
ATCATGGCCCCGCGCCGCCGCTGGGGGGTAACTGTGGATCACGCGGCACGTTTGGCGAAAAATCGCCCGCGCCCGGGGTC
TTGGCAAAGGGAGAGGCCGG

Downstream 100 bases:

>100_bases
TATCGCCGGGTCCCTCTATTCGCGCAAGACTGCCGAGCGCAGGACCTTTTCCAGCCCGGCCCAGGGATCGCCGCCTAAAG
CCCCGCCTTCGGGCGGGGCG

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 658; Mature: 658

Protein sequence:

>658_residues
MCGLTGYWTRGGDGRGRGEMTALVLRMAGALAHRGPDGDGAWVDEDAGLALGHRRLSIVDLSPAGQQPMVSADGRLALAY
NGEIYNAAELRPELEAAGIVFRGHSDTEVLLEGFARWGVVPTLRRAIGMFAFALWDRKERRLTLGRDRLGIKPLYVADHG
GTLLFASQPKGMMPHPSWRGEVDREGLAAMLRLSCLPAPRSIFKGVSQLAPGTVVRFEADGRRSEEVFWSLEEVAARGVA
QARSQPARPESASLAVEHLESLLLDAVRRRLIADVPVGAFLSGGVDSSTVVALMRKVASGPVKTFSIGFAEEGWDESVHA
AAVARHLGTDHRELVVSPDEVPAIVGDLPRWYDEPFADASQIPTLLVSKLARSEVTVSLSGDGGDELFAGYNRHVQGQRL
WDLAQRIPTVGRDLGAALIEAPSPGFWQRAVGLLPAGRRPPQAADKPAKIARLLRARSPEALYRTLVGAMPSAEVPLAGV
SLAAEERWYAASAEGVDSFVDRMRLLDAKGYLPDDILTKVDRASMAYGLEARVPLLDHRVVEFAWSLPPEMLIREGQGKW
LLRRVLHRHVPRALIERPKMGFGVPLAAWLRGPLRGWAEDLLDPQTMAVQGLIDPAPVRRWWLDHLAGRRDTHDRLWPVL
SALSWHHHWKSAGAFTAA

Sequences:

>Translated_658_residues
MCGLTGYWTRGGDGRGRGEMTALVLRMAGALAHRGPDGDGAWVDEDAGLALGHRRLSIVDLSPAGQQPMVSADGRLALAY
NGEIYNAAELRPELEAAGIVFRGHSDTEVLLEGFARWGVVPTLRRAIGMFAFALWDRKERRLTLGRDRLGIKPLYVADHG
GTLLFASQPKGMMPHPSWRGEVDREGLAAMLRLSCLPAPRSIFKGVSQLAPGTVVRFEADGRRSEEVFWSLEEVAARGVA
QARSQPARPESASLAVEHLESLLLDAVRRRLIADVPVGAFLSGGVDSSTVVALMRKVASGPVKTFSIGFAEEGWDESVHA
AAVARHLGTDHRELVVSPDEVPAIVGDLPRWYDEPFADASQIPTLLVSKLARSEVTVSLSGDGGDELFAGYNRHVQGQRL
WDLAQRIPTVGRDLGAALIEAPSPGFWQRAVGLLPAGRRPPQAADKPAKIARLLRARSPEALYRTLVGAMPSAEVPLAGV
SLAAEERWYAASAEGVDSFVDRMRLLDAKGYLPDDILTKVDRASMAYGLEARVPLLDHRVVEFAWSLPPEMLIREGQGKW
LLRRVLHRHVPRALIERPKMGFGVPLAAWLRGPLRGWAEDLLDPQTMAVQGLIDPAPVRRWWLDHLAGRRDTHDRLWPVL
SALSWHHHWKSAGAFTAA
>Mature_658_residues
MCGLTGYWTRGGDGRGRGEMTALVLRMAGALAHRGPDGDGAWVDEDAGLALGHRRLSIVDLSPAGQQPMVSADGRLALAY
NGEIYNAAELRPELEAAGIVFRGHSDTEVLLEGFARWGVVPTLRRAIGMFAFALWDRKERRLTLGRDRLGIKPLYVADHG
GTLLFASQPKGMMPHPSWRGEVDREGLAAMLRLSCLPAPRSIFKGVSQLAPGTVVRFEADGRRSEEVFWSLEEVAARGVA
QARSQPARPESASLAVEHLESLLLDAVRRRLIADVPVGAFLSGGVDSSTVVALMRKVASGPVKTFSIGFAEEGWDESVHA
AAVARHLGTDHRELVVSPDEVPAIVGDLPRWYDEPFADASQIPTLLVSKLARSEVTVSLSGDGGDELFAGYNRHVQGQRL
WDLAQRIPTVGRDLGAALIEAPSPGFWQRAVGLLPAGRRPPQAADKPAKIARLLRARSPEALYRTLVGAMPSAEVPLAGV
SLAAEERWYAASAEGVDSFVDRMRLLDAKGYLPDDILTKVDRASMAYGLEARVPLLDHRVVEFAWSLPPEMLIREGQGKW
LLRRVLHRHVPRALIERPKMGFGVPLAAWLRGPLRGWAEDLLDPQTMAVQGLIDPAPVRRWWLDHLAGRRDTHDRLWPVL
SALSWHHHWKSAGAFTAA

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=410, Percent_Identity=28.5365853658537, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI168229252, Length=410, Percent_Identity=28.5365853658537, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI168229250, Length=410, Percent_Identity=28.5365853658537, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI296010848, Length=380, Percent_Identity=29.4736842105263, Blast_Score=120, Evalue=4e-27,
Organism=Escherichia coli, GI1786889, Length=410, Percent_Identity=30.2439024390244, Blast_Score=136, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI25147557, Length=473, Percent_Identity=26.6384778012685, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71993933, Length=473, Percent_Identity=26.6384778012685, Blast_Score=110, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321563, Length=402, Percent_Identity=27.6119402985075, Blast_Score=106, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6325403, Length=435, Percent_Identity=27.3563218390805, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI45553209, Length=408, Percent_Identity=32.1078431372549, Blast_Score=165, Evalue=8e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 71903; Mature: 71903

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGLTGYWTRGGDGRGRGEMTALVLRMAGALAHRGPDGDGAWVDEDAGLALGHRRLSIVD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCEECCCEEEEEE
LSPAGQQPMVSADGRLALAYNGEIYNAAELRPELEAAGIVFRGHSDTEVLLEGFARWGVV
CCCCCCCCEECCCCEEEEEECCCEECHHHCCCCHHHCCEEEECCCCHHHHHHHHHHHCHH
PTLRRAIGMFAFALWDRKERRLTLGRDRLGIKPLYVADHGGTLLFASQPKGMMPHPSWRG
HHHHHHHHHHHHHHHCCHHHHEECCCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCC
EVDREGLAAMLRLSCLPAPRSIFKGVSQLAPGTVVRFEADGRRSEEVFWSLEEVAARGVA
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
QARSQPARPESASLAVEHLESLLLDAVRRRLIADVPVGAFLSGGVDSSTVVALMRKVASG
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHCC
PVKTFSIGFAEEGWDESVHAAAVARHLGTDHRELVVSPDEVPAIVGDLPRWYDEPFADAS
CCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHCCHHHHCCCCCCHH
QIPTLLVSKLARSEVTVSLSGDGGDELFAGYNRHVQGQRLWDLAQRIPTVGRDLGAALIE
HHHHHHHHHHHHCCEEEEECCCCCHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHC
APSPGFWQRAVGLLPAGRRPPQAADKPAKIARLLRARSPEALYRTLVGAMPSAEVPLAGV
CCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCC
SLAAEERWYAASAEGVDSFVDRMRLLDAKGYLPDDILTKVDRASMAYGLEARVPLLDHRV
CHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHH
VEFAWSLPPEMLIREGQGKWLLRRVLHRHVPRALIERPKMGFGVPLAAWLRGPLRGWAED
HHHHHCCCHHHHHCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
LLDPQTMAVQGLIDPAPVRRWWLDHLAGRRDTHDRLWPVLSALSWHHHWKSAGAFTAA
HCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MCGLTGYWTRGGDGRGRGEMTALVLRMAGALAHRGPDGDGAWVDEDAGLALGHRRLSIVD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCEECCCEEEEEE
LSPAGQQPMVSADGRLALAYNGEIYNAAELRPELEAAGIVFRGHSDTEVLLEGFARWGVV
CCCCCCCCEECCCCEEEEEECCCEECHHHCCCCHHHCCEEEECCCCHHHHHHHHHHHCHH
PTLRRAIGMFAFALWDRKERRLTLGRDRLGIKPLYVADHGGTLLFASQPKGMMPHPSWRG
HHHHHHHHHHHHHHHCCHHHHEECCCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCC
EVDREGLAAMLRLSCLPAPRSIFKGVSQLAPGTVVRFEADGRRSEEVFWSLEEVAARGVA
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
QARSQPARPESASLAVEHLESLLLDAVRRRLIADVPVGAFLSGGVDSSTVVALMRKVASG
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHCC
PVKTFSIGFAEEGWDESVHAAAVARHLGTDHRELVVSPDEVPAIVGDLPRWYDEPFADAS
CCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHCCHHHHCCCCCCHH
QIPTLLVSKLARSEVTVSLSGDGGDELFAGYNRHVQGQRLWDLAQRIPTVGRDLGAALIE
HHHHHHHHHHHHCCEEEEECCCCCHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHC
APSPGFWQRAVGLLPAGRRPPQAADKPAKIARLLRARSPEALYRTLVGAMPSAEVPLAGV
CCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCC
SLAAEERWYAASAEGVDSFVDRMRLLDAKGYLPDDILTKVDRASMAYGLEARVPLLDHRV
CHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHH
VEFAWSLPPEMLIREGQGKWLLRRVLHRHVPRALIERPKMGFGVPLAAWLRGPLRGWAED
HHHHHCCCHHHHHCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
LLDPQTMAVQGLIDPAPVRRWWLDHLAGRRDTHDRLWPVLSALSWHHHWKSAGAFTAA
HCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]