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The map label for this gene is pleD [H]

Identifier: 83591425

GI number: 83591425

Start: 98274

End: 99635

Strand: Direct

Name: pleD [H]

Synonym: Rru_A0085

Alternate gene names: 83591425

Gene position: 98274-99635 (Clockwise)

Preceding gene: 83591424

Following gene: 83591426

Centisome position: 2.26

GC content: 61.01

Gene sequence:

>1362_bases
ATGGATCTGCTGACCGGCAGCGATGCCGACATCGAAATCGAAGAGGCGCCGCCGGGAAAGATCCTGATCGTCGAGGATTC
GCCCTTCTTTATGCATGTCCTGCGCCGGCGGATCGGTCAGGAACTGGGCGTTCCGATCATCGAGGCCGAAACCCTGGCCC
AGGCCGCCCTGATCATCGAAGAGGAAGGGTCCAATCTATTCCTCTGCCTGATCGATCTGGTGCTGCCCGATGCGCCGAAC
GGCGAGATCGTCGATTATGTGACCGGGAAAGGTCTGGCCGCGGTGGTGTTCACCGGCGCCTTCAGCGAGGAAATGCGCGA
GCATGTCCTGTCTAGCGCCATTATCGATTATGTTACCAAGGATAGTCTGGCCAGCCTGGACTATCTGGTGGCGATCGTCC
GCCGGCTTTGCCGCAACGCCCACCAGAAAGCCATCGTCGTCGATGACAGCCGTACCGCCCGGGCTTATATCGGCGATCTG
CTCCGCCTGTATCGTTTCAATGTCATCGAGGCGGTCGACGGCGAGGAGGCCCTGGCCCTGCTGGCGGCGAACCCCGATAT
CCGGCTGATGGTCACCGATTATTACATGCCCAGAATCGATGGCTTCGAATTGATCAAACGCGTGCGCCAAACCCATTCGC
GCAGCGCTCTGGCGATCATCGGCGTGTCCACCGGGGGAAGCGCGCCGCTTTCGGCGCGGTTCATCAAGTACGGGGCCAAT
GATTTCATCAACAAGCCCTTTCTGCGCGAGGAATTCTTCTGCCGCATCGCCCAGAACATGGACACCTTGGATTATATCGC
CGCCCTGGCCCGCTCGGCCCAGATCGATCCGCTGACCGGACTGCCCAACCGCCGCTATCTGTTCGATGTCGCCAGCCGCG
CCCTGGCCGCGACCTTGCGCGTCGGCGGTCATCCGGTGGTGGCGATGATCGATGCCGATCATTTCAAGGAAATAAACGAT
CGTTTCGGCCATGATGTCGGCGATCAGGTGCTTGAAGCCCTGGCTCGGGTGATGGACGATCATGTCCAGCGGAAGTCCGA
TGTCCTTGCCCGTTTCGGCGGCGAGGAGTTTTGCGTGTTCGTCTCCGACATGTCGCCCGAACAGGCCTTCACCTTTTTCG
ATGGCTTGCGCGCTGCTGTCGCCCAATCGCCGCTGTCGGTTCCCGGACTGGATGATTTGTCGCTGACCATCAGCATCGGC
GTTTCCACCTGCGCCGCCCCCGACTTGCGTACGATGATCCACAGCGCCGACCGTTTGCTCTATGACGCCAAGGCGGCGGG
GCGTGATTGCACGGTGGTCGAGGGCTTGGGCGAGCGGACGCAATCGTGCCGGCAAAGTAGCGGGAAGGACGAAGACGCGT
GA

Upstream 100 bases:

>100_bases
AGTCAGGGTTGATCGGTCTCGCGATCGTGCTGGTCACCGCCAATCGGATGTCATAGGGTCAGAAGATTCGTTTCCCTCCC
GCGACAGACCGCACCCCCCT

Downstream 100 bases:

>100_bases
TGGAGGTGTTTATCGATGCCGATGCCTGCCCGGTGCGGACCGAGGCCTTCAAGGTCGCCGAGCGCCACCGCCTGATCGTG
CATGTGGTTGGCAATGCCCC

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MDLLTGSDADIEIEEAPPGKILIVEDSPFFMHVLRRRIGQELGVPIIEAETLAQAALIIEEEGSNLFLCLIDLVLPDAPN
GEIVDYVTGKGLAAVVFTGAFSEEMREHVLSSAIIDYVTKDSLASLDYLVAIVRRLCRNAHQKAIVVDDSRTARAYIGDL
LRLYRFNVIEAVDGEEALALLAANPDIRLMVTDYYMPRIDGFELIKRVRQTHSRSALAIIGVSTGGSAPLSARFIKYGAN
DFINKPFLREEFFCRIAQNMDTLDYIAALARSAQIDPLTGLPNRRYLFDVASRALAATLRVGGHPVVAMIDADHFKEIND
RFGHDVGDQVLEALARVMDDHVQRKSDVLARFGGEEFCVFVSDMSPEQAFTFFDGLRAAVAQSPLSVPGLDDLSLTISIG
VSTCAAPDLRTMIHSADRLLYDAKAAGRDCTVVEGLGERTQSCRQSSGKDEDA

Sequences:

>Translated_453_residues
MDLLTGSDADIEIEEAPPGKILIVEDSPFFMHVLRRRIGQELGVPIIEAETLAQAALIIEEEGSNLFLCLIDLVLPDAPN
GEIVDYVTGKGLAAVVFTGAFSEEMREHVLSSAIIDYVTKDSLASLDYLVAIVRRLCRNAHQKAIVVDDSRTARAYIGDL
LRLYRFNVIEAVDGEEALALLAANPDIRLMVTDYYMPRIDGFELIKRVRQTHSRSALAIIGVSTGGSAPLSARFIKYGAN
DFINKPFLREEFFCRIAQNMDTLDYIAALARSAQIDPLTGLPNRRYLFDVASRALAATLRVGGHPVVAMIDADHFKEIND
RFGHDVGDQVLEALARVMDDHVQRKSDVLARFGGEEFCVFVSDMSPEQAFTFFDGLRAAVAQSPLSVPGLDDLSLTISIG
VSTCAAPDLRTMIHSADRLLYDAKAAGRDCTVVEGLGERTQSCRQSSGKDEDA
>Mature_453_residues
MDLLTGSDADIEIEEAPPGKILIVEDSPFFMHVLRRRIGQELGVPIIEAETLAQAALIIEEEGSNLFLCLIDLVLPDAPN
GEIVDYVTGKGLAAVVFTGAFSEEMREHVLSSAIIDYVTKDSLASLDYLVAIVRRLCRNAHQKAIVVDDSRTARAYIGDL
LRLYRFNVIEAVDGEEALALLAANPDIRLMVTDYYMPRIDGFELIKRVRQTHSRSALAIIGVSTGGSAPLSARFIKYGAN
DFINKPFLREEFFCRIAQNMDTLDYIAALARSAQIDPLTGLPNRRYLFDVASRALAATLRVGGHPVVAMIDADHFKEIND
RFGHDVGDQVLEALARVMDDHVQRKSDVLARFGGEEFCVFVSDMSPEQAFTFFDGLRAAVAQSPLSVPGLDDLSLTISIG
VSTCAAPDLRTMIHSADRLLYDAKAAGRDCTVVEGLGERTQSCRQSSGKDEDA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=197, Percent_Identity=33.502538071066, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI87082007, Length=179, Percent_Identity=37.4301675977654, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786584, Length=161, Percent_Identity=39.1304347826087, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787262, Length=169, Percent_Identity=36.6863905325444, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87081881, Length=162, Percent_Identity=34.5679012345679, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1787802, Length=179, Percent_Identity=32.9608938547486, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI87081977, Length=172, Percent_Identity=31.3953488372093, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788085, Length=166, Percent_Identity=30.1204819277108, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1787541, Length=178, Percent_Identity=32.0224719101124, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1787816, Length=179, Percent_Identity=33.5195530726257, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081974, Length=94, Percent_Identity=37.2340425531915, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 49629; Mature: 49629

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00120 LIPASE_SER ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLLTGSDADIEIEEAPPGKILIVEDSPFFMHVLRRRIGQELGVPIIEAETLAQAALIIE
CCCCCCCCCCEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEHHHHHHEEEEEE
EEGSNLFLCLIDLVLPDAPNGEIVDYVTGKGLAAVVFTGAFSEEMREHVLSSAIIDYVTK
ECCCCEEEEEEHHHCCCCCCCCEEEEECCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHH
DSLASLDYLVAIVRRLCRNAHQKAIVVDDSRTARAYIGDLLRLYRFNVIEAVDGEEALAL
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHEECCCCHHEEE
LAANPDIRLMVTDYYMPRIDGFELIKRVRQTHSRSALAIIGVSTGGSAPLSARFIKYGAN
EECCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHCCH
DFINKPFLREEFFCRIAQNMDTLDYIAALARSAQIDPLTGLPNRRYLFDVASRALAATLR
HHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VGGHPVVAMIDADHFKEINDRFGHDVGDQVLEALARVMDDHVQRKSDVLARFGGEEFCVF
CCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
VSDMSPEQAFTFFDGLRAAVAQSPLSVPGLDDLSLTISIGVSTCAAPDLRTMIHSADRLL
EECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHH
YDAKAAGRDCTVVEGLGERTQSCRQSSGKDEDA
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDLLTGSDADIEIEEAPPGKILIVEDSPFFMHVLRRRIGQELGVPIIEAETLAQAALIIE
CCCCCCCCCCEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEHHHHHHEEEEEE
EEGSNLFLCLIDLVLPDAPNGEIVDYVTGKGLAAVVFTGAFSEEMREHVLSSAIIDYVTK
ECCCCEEEEEEHHHCCCCCCCCEEEEECCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHH
DSLASLDYLVAIVRRLCRNAHQKAIVVDDSRTARAYIGDLLRLYRFNVIEAVDGEEALAL
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHEECCCCHHEEE
LAANPDIRLMVTDYYMPRIDGFELIKRVRQTHSRSALAIIGVSTGGSAPLSARFIKYGAN
EECCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHCCH
DFINKPFLREEFFCRIAQNMDTLDYIAALARSAQIDPLTGLPNRRYLFDVASRALAATLR
HHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VGGHPVVAMIDADHFKEINDRFGHDVGDQVLEALARVMDDHVQRKSDVLARFGGEEFCVF
CCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
VSDMSPEQAFTFFDGLRAAVAQSPLSVPGLDDLSLTISIGVSTCAAPDLRTMIHSADRLL
EECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHH
YDAKAAGRDCTVVEGLGERTQSCRQSSGKDEDA
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA