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The map label for this gene is gcvA [H]

Identifier: 83591371

GI number: 83591371

Start: 34821

End: 35720

Strand: Direct

Name: gcvA [H]

Synonym: Rru_A0031

Alternate gene names: 83591371

Gene position: 34821-35720 (Clockwise)

Preceding gene: 83591367

Following gene: 83591382

Centisome position: 0.8

GC content: 60.67

Gene sequence:

>900_bases
ATGCGCCGCAAGATTCCCAGCAATTCTGCACTCATGGCCTTCGAGGCGTCGGCGCGTCATGGGAGCTTTGCTCGCGCGGC
TGACGAACTGGGCCTGACCGAAGGGGCGATCAGCCGCCAGATAGGCCGGCTGGAAGCTTTCCTCGGCGTCACGCTGTTCG
CGCGGGTTGGTAATCGTGTCCGGCTTTTGCCAAATGGCGAGCGTTATGCGGCTCAGGTGCGCGAGTCACTCGACCGACTG
GAGCGTGATAGTCGCTATCTGATGGGGCAACCCAGCGATGGCGCGAGCCTCGACATCGCCACCATCCCGACCTTCGCCGC
CCGCTGGCTCATTCCCCGCTTGGCACGGTTCAGGCAAGAGCACCCGAACATCATCGTGCATCTTGCCGAACGGATGGAGC
CCTTTGTGCTTGCCGGAAGCGGTTTCGATGCCGCGATCCATTTCGAGCATCCTGCTTGGACGGGAATGGGAACGCACCGT
CTGTTGCATGAGACCTTGGTTCCGGTTTGCCATCCGGCGCTTCTCGGGGGAGGGGAAGGAGCGGTATCCTTGGATGAATT
GCCGCGCCTTCATCGACGTCAGAACCCGGATGCTTGGCAACGCTACGCCCAGGAAACCGGACTCGTTCTGACCAATCCTG
CGATCGGTCCCCGTTATGATCTTCACATTATGCTGATCGAGGCCGCGCTCGCGGGACTTGGCGTCGCCCTGGTGCCGCGC
CTCTATATCGAAACCGAGTTGGCGGAAGGCCGATTGATCGCGCCTTGGCCTGATGGTCAGTCGATCTCCAAGACCTTCTG
CCTCATCCTGCCCGAGCCGATCGGATTGAGTGACGGTCCGCTACAGGTTTTTGCAAAGTGGCTTCTCAAGGAAGCGCGAT
CTGCAAATCCAACGCCTTGA

Upstream 100 bases:

>100_bases
GGCCGATATTCGCCCATATATCGACAACGGATCGCGCTTCAGTGATCTAATATCAAGGTGGTGGCGGATCGAATGCCGAG
CCATCCCGTCAGGAACCCTC

Downstream 100 bases:

>100_bases
TGCCAGCCCGCTGCCTCTGGCCCGCGCTTCGGCCCTGACAAACGGGGGGAACGAGCCCCCCTCTCTTTAGGCGGGGGGCA
GGGCGTCCAGGCGTTTGCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MRRKIPSNSALMAFEASARHGSFARAADELGLTEGAISRQIGRLEAFLGVTLFARVGNRVRLLPNGERYAAQVRESLDRL
ERDSRYLMGQPSDGASLDIATIPTFAARWLIPRLARFRQEHPNIIVHLAERMEPFVLAGSGFDAAIHFEHPAWTGMGTHR
LLHETLVPVCHPALLGGGEGAVSLDELPRLHRRQNPDAWQRYAQETGLVLTNPAIGPRYDLHIMLIEAALAGLGVALVPR
LYIETELAEGRLIAPWPDGQSISKTFCLILPEPIGLSDGPLQVFAKWLLKEARSANPTP

Sequences:

>Translated_299_residues
MRRKIPSNSALMAFEASARHGSFARAADELGLTEGAISRQIGRLEAFLGVTLFARVGNRVRLLPNGERYAAQVRESLDRL
ERDSRYLMGQPSDGASLDIATIPTFAARWLIPRLARFRQEHPNIIVHLAERMEPFVLAGSGFDAAIHFEHPAWTGMGTHR
LLHETLVPVCHPALLGGGEGAVSLDELPRLHRRQNPDAWQRYAQETGLVLTNPAIGPRYDLHIMLIEAALAGLGVALVPR
LYIETELAEGRLIAPWPDGQSISKTFCLILPEPIGLSDGPLQVFAKWLLKEARSANPTP
>Mature_299_residues
MRRKIPSNSALMAFEASARHGSFARAADELGLTEGAISRQIGRLEAFLGVTLFARVGNRVRLLPNGERYAAQVRESLDRL
ERDSRYLMGQPSDGASLDIATIPTFAARWLIPRLARFRQEHPNIIVHLAERMEPFVLAGSGFDAAIHFEHPAWTGMGTHR
LLHETLVPVCHPALLGGGEGAVSLDELPRLHRRQNPDAWQRYAQETGLVLTNPAIGPRYDLHIMLIEAALAGLGVALVPR
LYIETELAEGRLIAPWPDGQSISKTFCLILPEPIGLSDGPLQVFAKWLLKEARSANPTP

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=297, Percent_Identity=34.006734006734, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI1788706, Length=298, Percent_Identity=29.8657718120805, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1786448, Length=290, Percent_Identity=32.0689655172414, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI145693193, Length=294, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI157672245, Length=140, Percent_Identity=35.7142857142857, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32944; Mature: 32944

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRKIPSNSALMAFEASARHGSFARAADELGLTEGAISRQIGRLEAFLGVTLFARVGNRV
CCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE
RLLPNGERYAAQVRESLDRLERDSRYLMGQPSDGASLDIATIPTFAARWLIPRLARFRQE
EEEECCHHHHHHHHHHHHHHHHCCCEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHH
HPNIIVHLAERMEPFVLAGSGFDAAIHFEHPAWTGMGTHRLLHETLVPVCHPALLGGGEG
CCCCEEEEHHHCCCEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
AVSLDELPRLHRRQNPDAWQRYAQETGLVLTNPAIGPRYDLHIMLIEAALAGLGVALVPR
CCCHHHHHHHHHCCCCHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHHCHHHHHHH
LYIETELAEGRLIAPWPDGQSISKTFCLILPEPIGLSDGPLQVFAKWLLKEARSANPTP
HHHEEECCCCEEEECCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRRKIPSNSALMAFEASARHGSFARAADELGLTEGAISRQIGRLEAFLGVTLFARVGNRV
CCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE
RLLPNGERYAAQVRESLDRLERDSRYLMGQPSDGASLDIATIPTFAARWLIPRLARFRQE
EEEECCHHHHHHHHHHHHHHHHCCCEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHH
HPNIIVHLAERMEPFVLAGSGFDAAIHFEHPAWTGMGTHRLLHETLVPVCHPALLGGGEG
CCCCEEEEHHHCCCEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
AVSLDELPRLHRRQNPDAWQRYAQETGLVLTNPAIGPRYDLHIMLIEAALAGLGVALVPR
CCCHHHHHHHHHCCCCHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHHCHHHHHHH
LYIETELAEGRLIAPWPDGQSISKTFCLILPEPIGLSDGPLQVFAKWLLKEARSANPTP
HHHEEECCCCEEEECCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]