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The map label for this gene is sigH [H]

Identifier: 83591295

GI number: 83591295

Start: 2586641

End: 2587294

Strand: Reverse

Name: sigH [H]

Synonym: Moth_2479

Alternate gene names: 83591295

Gene position: 2587294-2586641 (Counterclockwise)

Preceding gene: 83591296

Following gene: 83591294

Centisome position: 98.42

GC content: 49.69

Gene sequence:

>654_bases
ATGAGCGTTAATGTACAGCAGGAATCCTGGCAACCATATGAGTTAATGGGTGATGAGGACATTGTTTACCAGGCCCAGGA
GGGTGATGAAGTAGCCCAAGAGTATTTGATAAATAAATACCGGAATTTTGTGCGGGCCAAAGCCCGGTCTTACTTCTTGA
TTGGCGCCGACCGGGAAGATATCGTCCAGGAAGGCATGATTGGTCTGTATAAGGCCATCCGCGATTTTCGGGGGGATAAA
CTGTCTTCCTTCCGGGCCTTTGCGGAGCTGTGTATTACCCGCCAGATTATCACGGCCATTAAAACGGCTACCCGGCAAAA
ACATATTCCCTTAAACTCATATGTTTCCTTGAATAAGCCCATTTACGACGAGGATTCGGACCGGACCTTGCTGGACATTA
TCTCCGGCACAAGGATTACCGATCCGGAGGAACTGATTATCAGCCGGGAGGAATTCGGGGACATCGAGGAGAAGATGGGG
GAGATTCTAAGTTCCCTGGAATGGAAGGTCCTGATGTCCTACCTGGAAGGTAAATCCTATCAGGAGATCGCCGTTGACCT
GAAACGCCATGTCAAATCCATTGATAACGCCCTCCAGCGGGTTAAACGCAAATTAGAGCGTTACCTGGAGCGGCGGGACG
ACGATACCCAGTAA

Upstream 100 bases:

>100_bases
TTGCGGTTCAGAGAGGTTTCTTGTATAATTAGAACTGTCGTTGGACAAGATTTCGGCCTCCTTGATTTATCCTTTGGAAA
TCCAGAGTGGAGGCGTTGAC

Downstream 100 bases:

>100_bases
TTTACATGAAAACAGGTCTTGACGTAAACCCCAAAGGGTGATAAAATAAACTTCGCATTTTGGAAAGAGGATATGGAGGG
GTTCCCGAGTTGGCCAAAGG

Product: RNA polymerase factor sigma-70

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MSVNVQQESWQPYELMGDEDIVYQAQEGDEVAQEYLINKYRNFVRAKARSYFLIGADREDIVQEGMIGLYKAIRDFRGDK
LSSFRAFAELCITRQIITAIKTATRQKHIPLNSYVSLNKPIYDEDSDRTLLDIISGTRITDPEELIISREEFGDIEEKMG
EILSSLEWKVLMSYLEGKSYQEIAVDLKRHVKSIDNALQRVKRKLERYLERRDDDTQ

Sequences:

>Translated_217_residues
MSVNVQQESWQPYELMGDEDIVYQAQEGDEVAQEYLINKYRNFVRAKARSYFLIGADREDIVQEGMIGLYKAIRDFRGDK
LSSFRAFAELCITRQIITAIKTATRQKHIPLNSYVSLNKPIYDEDSDRTLLDIISGTRITDPEELIISREEFGDIEEKMG
EILSSLEWKVLMSYLEGKSYQEIAVDLKRHVKSIDNALQRVKRKLERYLERRDDDTQ
>Mature_216_residues
SVNVQQESWQPYELMGDEDIVYQAQEGDEVAQEYLINKYRNFVRAKARSYFLIGADREDIVQEGMIGLYKAIRDFRGDKL
SSFRAFAELCITRQIITAIKTATRQKHIPLNSYVSLNKPIYDEDSDRTLLDIISGTRITDPEELIISREEFGDIEEKMGE
ILSSLEWKVLMSYLEGKSYQEIAVDLKRHVKSIDNALQRVKRKLERYLERRDDDTQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in the transition to post-exponential phase in the beginning of sporulation [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

None

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014218
- InterPro:   IPR016371
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 25386; Mature: 25255

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00622 HTH_LUXR_1 ; PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVNVQQESWQPYELMGDEDIVYQAQEGDEVAQEYLINKYRNFVRAKARSYFLIGADRED
CCCCCCCCCCCCHHHCCCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHCEEEEECCHHH
IVQEGMIGLYKAIRDFRGDKLSSFRAFAELCITRQIITAIKTATRQKHIPLNSYVSLNKP
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCC
IYDEDSDRTLLDIISGTRITDPEELIISREEFGDIEEKMGEILSSLEWKVLMSYLEGKSY
CCCCCCCCHHHHHHCCCCCCCHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCH
QEIAVDLKRHVKSIDNALQRVKRKLERYLERRDDDTQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SVNVQQESWQPYELMGDEDIVYQAQEGDEVAQEYLINKYRNFVRAKARSYFLIGADRED
CCCCCCCCCCCHHHCCCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHCEEEEECCHHH
IVQEGMIGLYKAIRDFRGDKLSSFRAFAELCITRQIITAIKTATRQKHIPLNSYVSLNKP
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCC
IYDEDSDRTLLDIISGTRITDPEELIISREEFGDIEEKMGEILSSLEWKVLMSYLEGKSY
CCCCCCCCHHHHHHCCCCCCCHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCH
QEIAVDLKRHVKSIDNALQRVKRKLERYLERRDDDTQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3277943; 6322121 [H]