BacMap

Definition	Moorella thermoacetica ATCC 39073, complete genome.
Accession	NC_007644
Length	2,628,784

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The map label for this gene is tuf

Identifier: 83591278

GI number: 83591278

Start: 2566951

End: 2568153

Strand: Reverse

Name: tuf

Synonym: Moth_2462

Alternate gene names: 83591278

Gene position: 2568153-2566951 (Counterclockwise)

Preceding gene: 83591279

Following gene: 83591277

Centisome position: 97.69

GC content: 56.11

Gene sequence:

>1203_bases
ATGGCCAAACAAAAATATGAACGCACGAAACCCCATGTCAACGTTGGTACCATCGGGCACGTTGACCATGGCAAGACGAC
CCTGACGGCAGCCATTACCTTCTGCCTGGCCAAGGCCGGCGGCGCTGTACCTACGGCCTACGATCAGATTGATAAGGCGC
CGGAAGAGAGAGAACGTGGAATTACCATCGCCACGGCCCACGTAGAGTACGAAACCGAGAAAAGGCACTATGCCCATGTT
GACTGCCCGGGTCACGCCGACTACGTCAAGAACATGATTACCGGTGCGGCACAGATGGACGGTGCCATCCTGGTAGTATC
AGCGGCCGACGGTCCCATGCCCCAGACCCGTGAACACATCCTTCTGGCCCGCCAGGTAGGCGTTCCTTACATCGTTGTTT
TCCTGAATAAAGTTGACCAGGTAGACGATCCCGAACTCCTGGAACTCGTGGAGATGGAAGTCCGGGAGCTTTTAACTGAA
TATGAATTCCCGGGCGATGAGATTCCTATTGTCACCGGCAGCGCCCTGAAAGCCATGGAATGCGGTTGCGGCAAACGGGA
ATGCGAATGGTGCGGCAAGGTGTGGGAGCTGATGGATGCCGTAGACAGCTATATTCCTACACCCGAGCGTGACACCGACA
AACCTTTCCTGATGCCCATTGAAGACGTCTTCACCATTACCGGCCGCGGTACCGTGACCACCGGCAGGGTGGAGAGGGGC
AAGGTCAAAGTCGGGGATGAAGTCGAGATTATCGGCCTGCGCGACGAGATCCGCAAGACGGTAGTCACCGGTGTGGAGAT
GTTCCGCAAGATCCTCGACGAAGCCGTTGCTGGTGACAATATCGGCACCCTCCTCCGTGGTGTCGACCGCAAAGAGGTCG
AGCGCGGTATGGTCCTGGCCAAACCGGGCAGCATTAAACCCCATACCAAATTCAATGCCGAGGTTTACGTCCTGACCAAA
GAGGAAGGTGGCCGGCATACTCCCTTCTTTAACGGCTACCGGCCCCAGTTCTATTTCCGGACTACCGACGTTACCGGCGT
GGTAAACCTCCCCGAAGGTGTAGAGATGGTCATGCCGGGCGATAACATCCGTATGACCATTGAGCTCATTACCCCCATTG
CTATTGAAGAAGGCCTGCGTTTCGCTATCCGCGAAGGTGGTCGTACTGTCGGCGCCGGCGTTGTTACCGGGATTATCGAA
TAA

Upstream 100 bases:

>100_bases
GACGATTTAGTTTTGAGTTGGGGAGGAAGATACCCTCCCCTGGAAACATAGCCCAAATTATAGAGCTAAAAGAGCTATAT
TTTAAGGAGGCTAAAAGACT

Downstream 100 bases:

>100_bases
TTAAGGAGCGGTAAGGGATCAGGCGTTACGCCTGATCCCGTGCCATGAAGGAGGGTAATTAACGCATGGCGAGGCAGAAG
ATACGTATTCGCTTAAAGGC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MAKQKYERTKPHVNVGTIGHVDHGKTTLTAAITFCLAKAGGAVPTAYDQIDKAPEERERGITIATAHVEYETEKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKVDQVDDPELLELVEMEVRELLTE
YEFPGDEIPIVTGSALKAMECGCGKRECEWCGKVWELMDAVDSYIPTPERDTDKPFLMPIEDVFTITGRGTVTTGRVERG
KVKVGDEVEIIGLRDEIRKTVVTGVEMFRKILDEAVAGDNIGTLLRGVDRKEVERGMVLAKPGSIKPHTKFNAEVYVLTK
EEGGRHTPFFNGYRPQFYFRTTDVTGVVNLPEGVEMVMPGDNIRMTIELITPIAIEEGLRFAIREGGRTVGAGVVTGIIE

Sequences:

>Translated_400_residues
MAKQKYERTKPHVNVGTIGHVDHGKTTLTAAITFCLAKAGGAVPTAYDQIDKAPEERERGITIATAHVEYETEKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKVDQVDDPELLELVEMEVRELLTE
YEFPGDEIPIVTGSALKAMECGCGKRECEWCGKVWELMDAVDSYIPTPERDTDKPFLMPIEDVFTITGRGTVTTGRVERG
KVKVGDEVEIIGLRDEIRKTVVTGVEMFRKILDEAVAGDNIGTLLRGVDRKEVERGMVLAKPGSIKPHTKFNAEVYVLTK
EEGGRHTPFFNGYRPQFYFRTTDVTGVVNLPEGVEMVMPGDNIRMTIELITPIAIEEGLRFAIREGGRTVGAGVVTGIIE
>Mature_399_residues
AKQKYERTKPHVNVGTIGHVDHGKTTLTAAITFCLAKAGGAVPTAYDQIDKAPEERERGITIATAHVEYETEKRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKVDQVDDPELLELVEMEVRELLTEY
EFPGDEIPIVTGSALKAMECGCGKRECEWCGKVWELMDAVDSYIPTPERDTDKPFLMPIEDVFTITGRGTVTTGRVERGK
VKVGDEVEIIGLRDEIRKTVVTGVEMFRKILDEAVAGDNIGTLLRGVDRKEVERGMVLAKPGSIKPHTKFNAEVYVLTKE
EGGRHTPFFNGYRPQFYFRTTDVTGVVNLPEGVEMVMPGDNIRMTIELITPIAIEEGLRFAIREGGRTVGAGVVTGIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=398, Percent_Identity=58.0402010050251, Blast_Score=476, Evalue=1e-134,
Organism=Homo sapiens, GI223555963, Length=443, Percent_Identity=30.6997742663657, Blast_Score=175, Evalue=7e-44,
Organism=Homo sapiens, GI5729864, Length=443, Percent_Identity=30.6997742663657, Blast_Score=175, Evalue=8e-44,
Organism=Homo sapiens, GI4503471, Length=445, Percent_Identity=31.0112359550562, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=29.2410714285714, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI54607086, Length=349, Percent_Identity=30.0859598853868, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI46094014, Length=352, Percent_Identity=28.4090909090909, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI194018522, Length=339, Percent_Identity=28.6135693215339, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI194018520, Length=339, Percent_Identity=28.6135693215339, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI194097354, Length=339, Percent_Identity=28.6135693215339, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI4503507, Length=275, Percent_Identity=27.2727272727273, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI157426893, Length=242, Percent_Identity=28.099173553719, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI94966754, Length=138, Percent_Identity=32.6086956521739, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1790412, Length=400, Percent_Identity=74.25, Blast_Score=588, Evalue=1e-169,
Organism=Escherichia coli, GI1789737, Length=399, Percent_Identity=74.4360902255639, Blast_Score=587, Evalue=1e-169,
Organism=Escherichia coli, GI2367247, Length=388, Percent_Identity=30.4123711340206, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI48994988, Length=255, Percent_Identity=32.156862745098, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1789108, Length=370, Percent_Identity=27.5675675675676, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789738, Length=146, Percent_Identity=33.5616438356164, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1788922, Length=140, Percent_Identity=34.2857142857143, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=402, Percent_Identity=51.7412935323383, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI25141371, Length=394, Percent_Identity=43.6548223350254, Blast_Score=321, Evalue=4e-88,
Organism=Caenorhabditis elegans, GI17552884, Length=453, Percent_Identity=31.1258278145695, Blast_Score=171, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17569207, Length=453, Percent_Identity=31.1258278145695, Blast_Score=171, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI32566303, Length=431, Percent_Identity=30.6264501160093, Blast_Score=148, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI32566629, Length=459, Percent_Identity=27.6688453159041, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI115532067, Length=440, Percent_Identity=28.8636363636364, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=437, Percent_Identity=28.8329519450801, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17506081, Length=277, Percent_Identity=31.0469314079422, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI32566301, Length=150, Percent_Identity=38.6666666666667, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17557151, Length=239, Percent_Identity=29.7071129707113, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17509919, Length=301, Percent_Identity=27.5747508305648, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=399, Percent_Identity=66.4160401002506, Blast_Score=538, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6325337, Length=446, Percent_Identity=31.390134529148, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6319594, Length=446, Percent_Identity=31.390134529148, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6320377, Length=346, Percent_Identity=28.0346820809249, Blast_Score=106, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6322937, Length=350, Percent_Identity=25.7142857142857, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6323320, Length=250, Percent_Identity=29.2, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=178, Percent_Identity=29.2134831460674, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363316, Length=399, Percent_Identity=57.3934837092732, Blast_Score=463, Evalue=1e-130,
Organism=Drosophila melanogaster, GI17864358, Length=399, Percent_Identity=57.3934837092732, Blast_Score=463, Evalue=1e-130,
Organism=Drosophila melanogaster, GI19921738, Length=390, Percent_Identity=47.9487179487179, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI45553807, Length=457, Percent_Identity=30.6345733041575, Blast_Score=171, Evalue=9e-43,
Organism=Drosophila melanogaster, GI45553816, Length=457, Percent_Identity=30.6345733041575, Blast_Score=171, Evalue=9e-43,
Organism=Drosophila melanogaster, GI24651721, Length=457, Percent_Identity=30.6345733041575, Blast_Score=171, Evalue=9e-43,
Organism=Drosophila melanogaster, GI17864154, Length=457, Percent_Identity=30.6345733041575, Blast_Score=171, Evalue=9e-43,
Organism=Drosophila melanogaster, GI24652838, Length=447, Percent_Identity=30.6487695749441, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI17137572, Length=447, Percent_Identity=30.6487695749441, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI19922690, Length=309, Percent_Identity=32.3624595469256, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI45550900, Length=450, Percent_Identity=28.4444444444444, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI17137380, Length=342, Percent_Identity=29.8245614035088, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24647048, Length=284, Percent_Identity=25.3521126760563, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24647046, Length=284, Percent_Identity=25.3521126760563, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI78706572, Length=249, Percent_Identity=27.3092369477912, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28572034, Length=166, Percent_Identity=36.7469879518072, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_MOOTA (Q2RFP5)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_431287.1
- HSSP:   P0A6N1
- ProteinModelPortal:   Q2RFP5
- SMR:   Q2RFP5
- STRING:   Q2RFP5
- GeneID:   3832212
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_2462
- NMPDR:   fig|264732.9.peg.2494
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   AACIEEL
- PhylomeDB:   Q2RFP5
- ProtClustDB:   PRK00049
- BioCyc:   MTHE264732:MOTH_2462-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 44125; Mature: 43994

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQKYERTKPHVNVGTIGHVDHGKTTLTAAITFCLAKAGGAVPTAYDQIDKAPEERERG
CCCCCCHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHCC
ITIATAHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIVVFLNKVDQVDDPELLELVEMEVRELLTEYEFPGDEIPIVTGSALKAME
HHHHHCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHH
CGCGKRECEWCGKVWELMDAVDSYIPTPERDTDKPFLMPIEDVFTITGRGTVTTGRVERG
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHEEEEECCCEEEECCEECC
KVKVGDEVEIIGLRDEIRKTVVTGVEMFRKILDEAVAGDNIGTLLRGVDRKEVERGMVLA
EEEECCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHCCEEEE
KPGSIKPHTKFNAEVYVLTKEEGGRHTPFFNGYRPQFYFRTTDVTGVVNLPEGVEMVMPG
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECC
DNIRMTIELITPIAIEEGLRFAIREGGRTVGAGVVTGIIE
CCEEEEEEEECCHHHHHCHHHHHHCCCCEECCHHHHEECC
>Mature Secondary Structure 
AKQKYERTKPHVNVGTIGHVDHGKTTLTAAITFCLAKAGGAVPTAYDQIDKAPEERERG
CCCCCHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHCC
ITIATAHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIVVFLNKVDQVDDPELLELVEMEVRELLTEYEFPGDEIPIVTGSALKAME
HHHHHCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHH
CGCGKRECEWCGKVWELMDAVDSYIPTPERDTDKPFLMPIEDVFTITGRGTVTTGRVERG
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHEEEEECCCEEEECCEECC
KVKVGDEVEIIGLRDEIRKTVVTGVEMFRKILDEAVAGDNIGTLLRGVDRKEVERGMVLA
EEEECCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHCCEEEE
KPGSIKPHTKFNAEVYVLTKEEGGRHTPFFNGYRPQFYFRTTDVTGVVNLPEGVEMVMPG
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECC
DNIRMTIELITPIAIEEGLRFAIREGGRTVGAGVVTGIIE
CCEEEEEEEECCHHHHHCHHHHHHCCCCEECCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA