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The map label for this gene is prfA

Identifier: 83591213

GI number: 83591213

Start: 2517644

End: 2518714

Strand: Reverse

Name: prfA

Synonym: Moth_2397

Alternate gene names: 83591213

Gene position: 2518714-2517644 (Counterclockwise)

Preceding gene: 83591214

Following gene: 83591212

Centisome position: 95.81

GC content: 60.78

Gene sequence:

>1071_bases
ATGCTGGAAAAACTGGAACAGATCGAAGCCAGATACGAAGAACTGGGCCGGCTGATGGGTGACCCGGAAGTAATAGCCGA
CCCCGAGCAATTACAGAAACACGCCCGGGCCCACGCAGCCCTGGAAGATATAGTCACCACCTTCCGCCGGTACCGCCAGG
TCAGCAATGAGCTGGCAGAAGATAAAGCCATGCTGGAGGAAGAAAAAGACCGGGAATTCCAGGAACTCCTCAAGGCCGAG
ATTGAACGCCTGACCGGGGAGCAGGAACGCCTGGAACAGGAGTTGAAGATCCTCCTCCTGCCCAAAGACCCCAATGATGA
AAAGGACATCATCATGGAGATCCGCGCCGGTGCCGGGGGCGAAGAGGCGGCCCTCTTTGCCGGCGATCTCTTCCGCATGT
ACCAGCGCTATGCCGAAAAGAAACGCTGGCGGACGGAGATTATCAGCTCCCACCCCACCGAACTGGGCGGTTTCAAGGAG
ATCATCTTCCAGGTCGAGGGGCAGGGGGTTTACAGCCGCCTGAAGTTTGAGAGCGGGGTACACCGGGTGCAGCGCATCCC
GACCACGGAATCCGGCGGGCGCATTCACACGTCAACGGCTACCGTGGCCGTGTTGCCCGAGGCGGAAGAGGTAGACGTGG
AGATCAAGCCCGAAGACCTGCGGGTGGACATCTTCTGTTCCAGCGGTCCCGGCGGCCAGTCGGTCAACACCACCTACTCC
GCCGTCCGCATTACCCACCTGCCGACGGGCCTGGTGGTCTCCTGCCAGGACGAGAAGTCTCAGTTAAAGAATAAAGAAAA
GGCCATGAGGGTGTTGCGCGCCCGGCTCCTGGATATGGCCCGGGCTGAGCGGGAAGGGGAGCTGGCTGAAGAGCGGCGCT
CCCAGGTAGGCAGCGGCGACCGCAGCGAGCGGATACGCACCTATAACTTCCCCCAGAACCGGGTGACGGACCACCGTATC
GGCCTGACCCTCCACCACCTGGACCAGGTCCTGGCAGGAGAACTGGACGAGATTATCGACGCCCTGGTCACCACCGACCA
GGCAGAACGCCTGAAAAACATGGAGGCCTGA

Upstream 100 bases:

>100_bases
AGCGTAACTCCTGAGGGGCTCAGGGGCAAATTCACCGGCAGTGCCGGAAGTTATCATGTGGCAGGCAACTTTTTTCGCAT
TAGAATCGAGGTGATGTCCG

Downstream 100 bases:

>100_bases
GATGACCCTGCGGCAAGCCCTGGGGGAGGCCGTCCGGCGCCTGGCGGCCGGGGGGGTTGAACGCCCGCGGCTGGAGGCGG
AAGTCCTTCTCGGGTGGGCC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MLEKLEQIEARYEELGRLMGDPEVIADPEQLQKHARAHAALEDIVTTFRRYRQVSNELAEDKAMLEEEKDREFQELLKAE
IERLTGEQERLEQELKILLLPKDPNDEKDIIMEIRAGAGGEEAALFAGDLFRMYQRYAEKKRWRTEIISSHPTELGGFKE
IIFQVEGQGVYSRLKFESGVHRVQRIPTTESGGRIHTSTATVAVLPEAEEVDVEIKPEDLRVDIFCSSGPGGQSVNTTYS
AVRITHLPTGLVVSCQDEKSQLKNKEKAMRVLRARLLDMARAEREGELAEERRSQVGSGDRSERIRTYNFPQNRVTDHRI
GLTLHHLDQVLAGELDEIIDALVTTDQAERLKNMEA

Sequences:

>Translated_356_residues
MLEKLEQIEARYEELGRLMGDPEVIADPEQLQKHARAHAALEDIVTTFRRYRQVSNELAEDKAMLEEEKDREFQELLKAE
IERLTGEQERLEQELKILLLPKDPNDEKDIIMEIRAGAGGEEAALFAGDLFRMYQRYAEKKRWRTEIISSHPTELGGFKE
IIFQVEGQGVYSRLKFESGVHRVQRIPTTESGGRIHTSTATVAVLPEAEEVDVEIKPEDLRVDIFCSSGPGGQSVNTTYS
AVRITHLPTGLVVSCQDEKSQLKNKEKAMRVLRARLLDMARAEREGELAEERRSQVGSGDRSERIRTYNFPQNRVTDHRI
GLTLHHLDQVLAGELDEIIDALVTTDQAERLKNMEA
>Mature_356_residues
MLEKLEQIEARYEELGRLMGDPEVIADPEQLQKHARAHAALEDIVTTFRRYRQVSNELAEDKAMLEEEKDREFQELLKAE
IERLTGEQERLEQELKILLLPKDPNDEKDIIMEIRAGAGGEEAALFAGDLFRMYQRYAEKKRWRTEIISSHPTELGGFKE
IIFQVEGQGVYSRLKFESGVHRVQRIPTTESGGRIHTSTATVAVLPEAEEVDVEIKPEDLRVDIFCSSGPGGQSVNTTYS
AVRITHLPTGLVVSCQDEKSQLKNKEKAMRVLRARLLDMARAEREGELAEERRSQVGSGDRSERIRTYNFPQNRVTDHRI
GLTLHHLDQVLAGELDEIIDALVTTDQAERLKNMEA

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=288, Percent_Identity=50, Blast_Score=285, Evalue=5e-77,
Organism=Homo sapiens, GI34577120, Length=320, Percent_Identity=41.25, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI166795305, Length=193, Percent_Identity=47.6683937823834, Blast_Score=187, Evalue=1e-47,
Organism=Escherichia coli, GI1787462, Length=355, Percent_Identity=53.8028169014084, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI2367172, Length=342, Percent_Identity=36.8421052631579, Blast_Score=216, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17542784, Length=339, Percent_Identity=35.9882005899705, Blast_Score=196, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6321295, Length=337, Percent_Identity=47.1810089020772, Blast_Score=266, Evalue=5e-72,
Organism=Drosophila melanogaster, GI19921226, Length=323, Percent_Identity=43.6532507739938, Blast_Score=242, Evalue=2e-64,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_MOOTA (Q2RFW0)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_431222.1
- HSSP:   P0A7I0
- ProteinModelPortal:   Q2RFW0
- SMR:   Q2RFW0
- STRING:   Q2RFW0
- GeneID:   3830764
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_2397
- NMPDR:   fig|264732.9.peg.2429
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- PhylomeDB:   Q2RFW0
- ProtClustDB:   PRK00591
- BioCyc:   MTHE264732:MOTH_2397-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40573; Mature: 40573

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEKLEQIEARYEELGRLMGDPEVIADPEQLQKHARAHAALEDIVTTFRRYRQVSNELAE
CCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKAMLEEEKDREFQELLKAEIERLTGEQERLEQELKILLLPKDPNDEKDIIMEIRAGAGG
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCEEEEECCCCCCCHHEEEEEECCCCC
EEAALFAGDLFRMYQRYAEKKRWRTEIISSHPTELGGFKEIIFQVEGQGVYSRLKFESGV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHH
HRVQRIPTTESGGRIHTSTATVAVLPEAEEVDVEIKPEDLRVDIFCSSGPGGQSVNTTYS
HHHHHCCCCCCCCEEEECCEEEEECCCCCCCCEEECCCCEEEEEEECCCCCCCCCCCHHH
AVRITHLPTGLVVSCQDEKSQLKNKEKAMRVLRARLLDMARAEREGELAEERRSQVGSGD
EEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
RSERIRTYNFPQNRVTDHRIGLTLHHLDQVLAGELDEIIDALVTTDQAERLKNMEA
CCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHCCCC
>Mature Secondary Structure
MLEKLEQIEARYEELGRLMGDPEVIADPEQLQKHARAHAALEDIVTTFRRYRQVSNELAE
CCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKAMLEEEKDREFQELLKAEIERLTGEQERLEQELKILLLPKDPNDEKDIIMEIRAGAGG
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCEEEEECCCCCCCHHEEEEEECCCCC
EEAALFAGDLFRMYQRYAEKKRWRTEIISSHPTELGGFKEIIFQVEGQGVYSRLKFESGV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHH
HRVQRIPTTESGGRIHTSTATVAVLPEAEEVDVEIKPEDLRVDIFCSSGPGGQSVNTTYS
HHHHHCCCCCCCCEEEECCEEEEECCCCCCCCEEECCCCEEEEEEECCCCCCCCCCCHHH
AVRITHLPTGLVVSCQDEKSQLKNKEKAMRVLRARLLDMARAEREGELAEERRSQVGSGD
EEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
RSERIRTYNFPQNRVTDHRIGLTLHHLDQVLAGELDEIIDALVTTDQAERLKNMEA
CCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA