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The map label for this gene is thiE

Identifier: 83590985

GI number: 83590985

Start: 2264451

End: 2265083

Strand: Reverse

Name: thiE

Synonym: Moth_2162

Alternate gene names: 83590985

Gene position: 2265083-2264451 (Counterclockwise)

Preceding gene: 83590986

Following gene: 83590984

Centisome position: 86.16

GC content: 63.03

Gene sequence:

>633_bases
ATGCCACAATGGGACCTTTATGTAGTCATCACCACCAAACTCGGTGGCGGCCGGCCGACCCTGGAGCTGGTGCGCGGGGC
CCTGGCCGGCGGGGCTACAGCCATCCAACTGCGGGAAAAGGAATTGCCGGCCCGGGAACTGGTGGAACTGGGCCGGGCCA
TCAGGGAATTGACCAGGGATGCCGGGGCTACCTTCATTGTCAACGACCGCCTGGACATAGCCCTGGCAGTCGAGGCCGAC
GGATTGCATATCGGTCAGGAAGATCTGCCCGCTCCGGTGGCCCGGAAGCTACTCGGGCCGGAAAAAATTCTTGGGGTATC
AGCGGGAACGACAGACGAGGCCCGGCAGGCCGAGGTCGACGGTGCCGACTACCTCGGGGTGGGCAGTATTTTTGCCACCG
GCAGCAAGGGGGATGCTGGTAGCCCTATTGGCCTGGAAGGATTGCGGGCCATTCGGGCTGCAGTTAAGATCCCTATAGTC
GGCATTGGCGGTATCAACCCTGATAATGCCGCCGGGGTAATAGCTGCCGGAGCCGATGGCGTATCGGTGATCTCGGCGGT
TATAGGGGCCGCGGACGTAGCCGCCGCCGCCCGCCGGCTTCGTGAGGTGGTAACCAGGGCCAGGGGAAAATAA

Upstream 100 bases:

>100_bases
GCAGGAAACTGGCACCCTGATAACGAAGTATAAAGCCGCCTGGCTATCCCACCGGGTGGCTTTTAAATTGCTGGGAGCGG
TTAAGGAGGAGACCAGGATG

Downstream 100 bases:

>100_bases
ATCAGCCGGCAGGATTATGGCTGCCGGGGGCGAAGAAATTAACCGGTGATCATTATGGACCTTAAGGGTCTTAAAGCAAA
AAGCCCGGCCTGCCGGGGCT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MPQWDLYVVITTKLGGGRPTLELVRGALAGGATAIQLREKELPARELVELGRAIRELTRDAGATFIVNDRLDIALAVEAD
GLHIGQEDLPAPVARKLLGPEKILGVSAGTTDEARQAEVDGADYLGVGSIFATGSKGDAGSPIGLEGLRAIRAAVKIPIV
GIGGINPDNAAGVIAAGADGVSVISAVIGAADVAAAARRLREVVTRARGK

Sequences:

>Translated_210_residues
MPQWDLYVVITTKLGGGRPTLELVRGALAGGATAIQLREKELPARELVELGRAIRELTRDAGATFIVNDRLDIALAVEAD
GLHIGQEDLPAPVARKLLGPEKILGVSAGTTDEARQAEVDGADYLGVGSIFATGSKGDAGSPIGLEGLRAIRAAVKIPIV
GIGGINPDNAAGVIAAGADGVSVISAVIGAADVAAAARRLREVVTRARGK
>Mature_209_residues
PQWDLYVVITTKLGGGRPTLELVRGALAGGATAIQLREKELPARELVELGRAIRELTRDAGATFIVNDRLDIALAVEADG
LHIGQEDLPAPVARKLLGPEKILGVSAGTTDEARQAEVDGADYLGVGSIFATGSKGDAGSPIGLEGLRAIRAAVKIPIVG
IGGINPDNAAGVIAAGADGVSVISAVIGAADVAAAARRLREVVTRARGK

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=185, Percent_Identity=32.4324324324324, Blast_Score=76, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325042, Length=224, Percent_Identity=36.1607142857143, Blast_Score=143, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_MOOTA (Q2RGI8)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_430994.1
- ProteinModelPortal:   Q2RGI8
- SMR:   Q2RGI8
- STRING:   Q2RGI8
- GeneID:   3833011
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_2162
- NMPDR:   fig|264732.9.peg.2188
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   IMGSNNC
- PhylomeDB:   Q2RGI8
- BioCyc:   MTHE264732:MOTH_2162-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 21409; Mature: 21278

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQWDLYVVITTKLGGGRPTLELVRGALAGGATAIQLREKELPARELVELGRAIRELTRD
CCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEHHCCCHHHHHHHHHHHHHHHHH
AGATFIVNDRLDIALAVEADGLHIGQEDLPAPVARKLLGPEKILGVSAGTTDEARQAEVD
CCCEEEEECCCEEEEEEECCCCEECCCCCCHHHHHHHCCHHHHEECCCCCCCHHHHHCCC
GADYLGVGSIFATGSKGDAGSPIGLEGLRAIRAAVKIPIVGIGGINPDNAAGVIAAGADG
CCCEEECCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEECCCH
VSVISAVIGAADVAAAARRLREVVTRARGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PQWDLYVVITTKLGGGRPTLELVRGALAGGATAIQLREKELPARELVELGRAIRELTRD
CCCEEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEHHCCCHHHHHHHHHHHHHHHHH
AGATFIVNDRLDIALAVEADGLHIGQEDLPAPVARKLLGPEKILGVSAGTTDEARQAEVD
CCCEEEEECCCEEEEEEECCCCEECCCCCCHHHHHHHCCHHHHEECCCCCCCHHHHHCCC
GADYLGVGSIFATGSKGDAGSPIGLEGLRAIRAAVKIPIVGIGGINPDNAAGVIAAGADG
CCCEEECCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEECCCH
VSVISAVIGAADVAAAARRLREVVTRARGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA