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The map label for this gene is yeaJ [H]

Identifier: 83590972

GI number: 83590972

Start: 2250281

End: 2251099

Strand: Reverse

Name: yeaJ [H]

Synonym: Moth_2149

Alternate gene names: 83590972

Gene position: 2251099-2250281 (Counterclockwise)

Preceding gene: 83590973

Following gene: 83590971

Centisome position: 85.63

GC content: 52.26

Gene sequence:

>819_bases
ATGAGTGTAGAGCAGGCTAAGGCCTTGTCTGAGTTCTGTAAAACTGTGATATATTTTCTATCAGCCATAGGCATGGGGAT
TTGCCTTCATAAAGGGCGTAACCGGAAAGGACGCGGGCCAGAGAAATTGTTGCTGCTAGGACTATTAACTATGGGTACCG
GCACTTTTCTGAGTGCGTACTGGGTCAAACTTGGTCTTCCCGAAAGCTGGGGAAGGATCCTGGGCGACCTGGTGCTGGTG
AACGCCGGCACGGCAGTGACTATATTGGCCATGTACTTCCTGACCCTAAAATTCCACCTGGTAGCCCTGGCGCTCAAGAA
AGAAGCAGTTACCGACCCTTTAACAGGCCTTTACAACCGCAGGACCTTTTTCCGGGAACTGGACTGCAGGTTAGAACGAG
GCGGCAGTTTTGCGGTGGCCGTCCTCGACCTGGATAACATGAAACAAATAAACGACACCCTGGGCCACCAGATAGGGGAT
GAAGTTTTGCGAGCAGCTGCAAGAGCCCTTAAAAAGGCAACACGTGAAAAAGACATAGCTGCCCGGTACGGGGGAGACGA
ATTTGTAGTTCTGTTTGCCGGTAAGGGCCCGCGAGTAGAAAAGTTCAAGGCCAGGCTTAAAGAGAACCTAATGGCGGAGC
TGCCTTATACTGGGGAGATTGAGGTTGGCCTTTCTACCGGGGTAGCCTACTGCCCCGAGGACGGCGAAGATGCCCGCTCT
CTTCTTTCCGCTGCTGACGCACGCATGTACGCTGAGAAAGAAGCCAAGAAGAACCGCTATTATGCGTTTGGCCTGCTGGC
TGAAAACAGTAAGCCATAG

Upstream 100 bases:

>100_bases
CGCGGCGGTAACAAACAAAAGCCTACCTCCCCAACAAGCTGAGTTCTTGGCGCGCGCTGCCAAATTGGTCAGCTCTAAAA
ATTGCGAGGTCATCTGAATA

Downstream 100 bases:

>100_bases
GAATTCGGAATAACCCGGAAGAGACCCTTTACAGTAGTGGAAGGGAGAAGGGATCTATTTTAGCAAGTCCGCTCGGTCAC
GCGCTACGGGTATGTTCTGA

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MSVEQAKALSEFCKTVIYFLSAIGMGICLHKGRNRKGRGPEKLLLLGLLTMGTGTFLSAYWVKLGLPESWGRILGDLVLV
NAGTAVTILAMYFLTLKFHLVALALKKEAVTDPLTGLYNRRTFFRELDCRLERGGSFAVAVLDLDNMKQINDTLGHQIGD
EVLRAAARALKKATREKDIAARYGGDEFVVLFAGKGPRVEKFKARLKENLMAELPYTGEIEVGLSTGVAYCPEDGEDARS
LLSAADARMYAEKEAKKNRYYAFGLLAENSKP

Sequences:

>Translated_272_residues
MSVEQAKALSEFCKTVIYFLSAIGMGICLHKGRNRKGRGPEKLLLLGLLTMGTGTFLSAYWVKLGLPESWGRILGDLVLV
NAGTAVTILAMYFLTLKFHLVALALKKEAVTDPLTGLYNRRTFFRELDCRLERGGSFAVAVLDLDNMKQINDTLGHQIGD
EVLRAAARALKKATREKDIAARYGGDEFVVLFAGKGPRVEKFKARLKENLMAELPYTGEIEVGLSTGVAYCPEDGEDARS
LLSAADARMYAEKEAKKNRYYAFGLLAENSKP
>Mature_271_residues
SVEQAKALSEFCKTVIYFLSAIGMGICLHKGRNRKGRGPEKLLLLGLLTMGTGTFLSAYWVKLGLPESWGRILGDLVLVN
AGTAVTILAMYFLTLKFHLVALALKKEAVTDPLTGLYNRRTFFRELDCRLERGGSFAVAVLDLDNMKQINDTLGHQIGDE
VLRAAARALKKATREKDIAARYGGDEFVVLFAGKGPRVEKFKARLKENLMAELPYTGEIEVGLSTGVAYCPEDGEDARSL
LSAADARMYAEKEAKKNRYYAFGLLAENSKP

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI87081974, Length=199, Percent_Identity=33.1658291457286, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1786584, Length=163, Percent_Identity=36.8098159509202, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787262, Length=161, Percent_Identity=32.2981366459627, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87082007, Length=193, Percent_Identity=32.6424870466321, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787541, Length=161, Percent_Identity=36.0248447204969, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87081977, Length=162, Percent_Identity=34.5679012345679, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI145693134, Length=176, Percent_Identity=31.25, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87081881, Length=166, Percent_Identity=34.3373493975904, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788085, Length=159, Percent_Identity=30.8176100628931, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787056, Length=155, Percent_Identity=34.1935483870968, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788381, Length=162, Percent_Identity=31.4814814814815, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787816, Length=189, Percent_Identity=31.2169312169312, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787802, Length=157, Percent_Identity=31.8471337579618, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788956, Length=159, Percent_Identity=34.5911949685535, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 29965; Mature: 29834

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVEQAKALSEFCKTVIYFLSAIGMGICLHKGRNRKGRGPEKLLLLGLLTMGTGTFLSAY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHH
WVKLGLPESWGRILGDLVLVNAGTAVTILAMYFLTLKFHLVALALKKEAVTDPLTGLYNR
HHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
RTFFRELDCRLERGGSFAVAVLDLDNMKQINDTLGHQIGDEVLRAAARALKKATREKDIA
HHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARYGGDEFVVLFAGKGPRVEKFKARLKENLMAELPYTGEIEVGLSTGVAYCPEDGEDARS
HHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCCCHHHHH
LLSAADARMYAEKEAKKNRYYAFGLLAENSKP
HHHHHHHHHHHHHHHHCCCEEEEEEEECCCCC
>Mature Secondary Structure 
SVEQAKALSEFCKTVIYFLSAIGMGICLHKGRNRKGRGPEKLLLLGLLTMGTGTFLSAY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHH
WVKLGLPESWGRILGDLVLVNAGTAVTILAMYFLTLKFHLVALALKKEAVTDPLTGLYNR
HHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
RTFFRELDCRLERGGSFAVAVLDLDNMKQINDTLGHQIGDEVLRAAARALKKATREKDIA
HHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARYGGDEFVVLFAGKGPRVEKFKARLKENLMAELPYTGEIEVGLSTGVAYCPEDGEDARS
HHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCCCHHHHH
LLSAADARMYAEKEAKKNRYYAFGLLAENSKP
HHHHHHHHHHHHHHHHCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]