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The map label for this gene is istB [H]

Identifier: 83590900

GI number: 83590900

Start: 2161936

End: 2162424

Strand: Reverse

Name: istB [H]

Synonym: Moth_2071

Alternate gene names: 83590900

Gene position: 2162424-2161936 (Counterclockwise)

Preceding gene: 83590902

Following gene: 83590899

Centisome position: 82.26

GC content: 51.74

Gene sequence:

>489_bases
ATGCTGTTATACCCCTGGCCCAAGCGGGACAGGAGGAAAGCAGATATTGTCATGATTGGTAACCCCGGAACCAGGAAAAC
CCATTTAAGTATCGCTCTGGGCCTTAAAGCCTGTGTCCACGGCTACTCGGTCCGCTTCTTTACCGCCGCCGGCCTGGTGA
CCAAACTGGCTGAAGCCCAGGACGAAAAAAGGCTGGGCCGCATGCTCAAAGAACTCTCCCGGGTTAACCTGTTAATCGTG
GATGAGCTCTCTTATATCTCCCTTCCCCGTCAGGGTGCAGAGTTGCTCTTTCAAGTTATTTCCGAAAGGAGTGAGCGTGG
GAGCATAATTATTAACACCAACCTGGATTTCTCCAAATGGGAGGAAATCTTTGGGGACCGTATGTTGGCAGCAGCCCTGG
TAGACCGGGTTACCTTTAAGTCCCACATCTTGAACATGAACGCTGAATCCTATCGCCTGAAACAGCTCCAAAAAGCCAGG
GCCAGCTAG

Upstream 100 bases:

>100_bases
TCATTGCTCTATCCCGCGGAAGCGTTTTTCGGCCATACCCTCCAGAAAGTGAGTTGCCGCCAGGCCGACAAGCACCAGCT
CCAGGGCCAGCAGGATCACT

Downstream 100 bases:

>100_bases
GACAGCTGTTCGTCGTTCGCGCGGCCTACCCCCTCCCTAGGGAAGGGGTAGGCACCATCTCTCCATCTCAACTTTTCCAC
ATTGAGTGGATCACTTTTTT

Product: IstB-like ATP-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MLLYPWPKRDRRKADIVMIGNPGTRKTHLSIALGLKACVHGYSVRFFTAAGLVTKLAEAQDEKRLGRMLKELSRVNLLIV
DELSYISLPRQGAELLFQVISERSERGSIIINTNLDFSKWEEIFGDRMLAAALVDRVTFKSHILNMNAESYRLKQLQKAR
AS

Sequences:

>Translated_162_residues
MLLYPWPKRDRRKADIVMIGNPGTRKTHLSIALGLKACVHGYSVRFFTAAGLVTKLAEAQDEKRLGRMLKELSRVNLLIV
DELSYISLPRQGAELLFQVISERSERGSIIINTNLDFSKWEEIFGDRMLAAALVDRVTFKSHILNMNAESYRLKQLQKAR
AS
>Mature_162_residues
MLLYPWPKRDRRKADIVMIGNPGTRKTHLSIALGLKACVHGYSVRFFTAAGLVTKLAEAQDEKRLGRMLKELSRVNLLIV
DELSYISLPRQGAELLFQVISERSERGSIIINTNLDFSKWEEIFGDRMLAAALVDRVTFKSHILNMNAESYRLKQLQKAR
AS

Specific function: One of two proteins expressed only when there is a tandem repeat of the IS21 insertion sequence, it is necessary for the transposition of plasmids with that tandem repeat [H]

COG id: COG1484

COG function: function code L; DNA replication protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS21/IS1162 putative ATP-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1787623, Length=145, Percent_Identity=29.6551724137931, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001270
- InterPro:   IPR002611 [H]

Pfam domain/function: PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 18447; Mature: 18447

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLYPWPKRDRRKADIVMIGNPGTRKTHLSIALGLKACVHGYSVRFFTAAGLVTKLAEAQ
CCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
DEKRLGRMLKELSRVNLLIVDELSYISLPRQGAELLFQVISERSERGSIIINTNLDFSKW
HHHHHHHHHHHHHHCCEEEEECCHHHCCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHH
EEIFGDRMLAAALVDRVTFKSHILNMNAESYRLKQLQKARAS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MLLYPWPKRDRRKADIVMIGNPGTRKTHLSIALGLKACVHGYSVRFFTAAGLVTKLAEAQ
CCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
DEKRLGRMLKELSRVNLLIVDELSYISLPRQGAELLFQVISERSERGSIIINTNLDFSKW
HHHHHHHHHHHHHHCCEEEEECCHHHCCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHH
EEIFGDRMLAAALVDRVTFKSHILNMNAESYRLKQLQKARAS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]