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The map label for this gene is aksA

Identifier: 83590770

GI number: 83590770

Start: 2012878

End: 2014029

Strand: Reverse

Name: aksA

Synonym: Moth_1938

Alternate gene names: 83590770

Gene position: 2014029-2012878 (Counterclockwise)

Preceding gene: 83590771

Following gene: 83590769

Centisome position: 76.61

GC content: 57.99

Gene sequence:

>1152_bases
ATGGGCGGACAGATTAAAATCGTCGATACCACCCTCCGGGACGGCGAACAAACAGCCGGGGTGGTCTTTGCCAACAGTGA
AAAACTGCGCATTGCCAAGATGCTGGACGCTATCGGTGTCGATCAAATTGAAGCCGGCGTACCGGTCATGGGCGGTGATG
AGAAAAAGGTCATCAAGGAGATTGTCGATGCCGGTTTGCGGGCGAGTATCATGGGCTGGAACCGGGCTGTCATTGACGAT
GTCCGGCATTCCATCGATTGTGGCTGCGATGCCGTAGCCATCTCCATTTCTACTTCTGATATCCATATCGAACACAAACT
GCGCAGCACCAGGGAAAAGGTCATTGAGTCCATGTCCCGGGCCTGCGAGTTCGCCAAGAAGCACAACCTCTACGTCTCCG
TCAATGCCGAGGACGCCAGCCGCACCGATCCCGATTACCTATTACAGTTTGCTCGGGCTGCCCGGGAGGCCGGCGCCGAC
CGCCTGCGCTTCTGCGATACGGTGGGCATAATGGATCCCTTCGGCACTTATGAAAAAATAAAATGGTTGATCGAAGAAGT
GGGCCTGGATGTAGAGATGCACATGCACAACGACTTTGGCATGGCAACGGCCAATACCCTGGCCGGCATCCGCGCCGGGG
CCAAGTACGCCGGGGTGACGGTGGTGGGCCTGGGCGAACGGGCCGGCAACGCCGCCCTGGAGGAAGTGGTCATGGCCTTA
AAATATCTGGAAAATATTGACTTAAAGTTTAAAACCGAGCAGTTCCGCGAGCTGGCCGAGTACGTTTCCCTGGCGGCACG
CCGGCAACTACCGGCCTGGAAAGCCGTTGTGGGCAGCAATATGTTTGCCCACGAGTCCGGTATTCATGCCGACGGCGCCC
TGAAGGACCCGCGCACCTACGAAGTAATGACGCCGGAAGAGGTCGGCCTGGAACGGCAGATTGTCATCGGCAAGCACTCG
GGCACGGCGGCCATTAAGGCCAAGTTTGCCGAGTACGGCGTGCACCTGGAAGAAGCAGACGCCGCAGCTATCCTCGCCAG
GGTGCGTGCCCTGGCGGTGGAACTCAAGCGCCCCCTCTTTGATAAGGAACTGGCCCACATCTATGAGGATTACCAGGAAG
CCCGGAAAAAGGCGGCCGTGACCGCCGTATAG

Upstream 100 bases:

>100_bases
GTATACTGGATTCTCGCTAATATCGGGCAGGAAACGTTATTTTTTTGCGGAATACATCTAGAATATGCCGGCGTAAAGCC
TTCAAAAAAGGGGTGAAAAA

Downstream 100 bases:

>100_bases
CCCGGCCCGGCTCCAGGGAAAGATAAACTATAGAAATTTTCCCTGGGAGGTAATCACCTTGGGCGATAAAAACCGCAGCC
GCTTTGTTAGCGACGAGGGC

Product: trans-homoaconitate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2 [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MGGQIKIVDTTLRDGEQTAGVVFANSEKLRIAKMLDAIGVDQIEAGVPVMGGDEKKVIKEIVDAGLRASIMGWNRAVIDD
VRHSIDCGCDAVAISISTSDIHIEHKLRSTREKVIESMSRACEFAKKHNLYVSVNAEDASRTDPDYLLQFARAAREAGAD
RLRFCDTVGIMDPFGTYEKIKWLIEEVGLDVEMHMHNDFGMATANTLAGIRAGAKYAGVTVVGLGERAGNAALEEVVMAL
KYLENIDLKFKTEQFRELAEYVSLAARRQLPAWKAVVGSNMFAHESGIHADGALKDPRTYEVMTPEEVGLERQIVIGKHS
GTAAIKAKFAEYGVHLEEADAAAILARVRALAVELKRPLFDKELAHIYEDYQEARKKAAVTAV

Sequences:

>Translated_383_residues
MGGQIKIVDTTLRDGEQTAGVVFANSEKLRIAKMLDAIGVDQIEAGVPVMGGDEKKVIKEIVDAGLRASIMGWNRAVIDD
VRHSIDCGCDAVAISISTSDIHIEHKLRSTREKVIESMSRACEFAKKHNLYVSVNAEDASRTDPDYLLQFARAAREAGAD
RLRFCDTVGIMDPFGTYEKIKWLIEEVGLDVEMHMHNDFGMATANTLAGIRAGAKYAGVTVVGLGERAGNAALEEVVMAL
KYLENIDLKFKTEQFRELAEYVSLAARRQLPAWKAVVGSNMFAHESGIHADGALKDPRTYEVMTPEEVGLERQIVIGKHS
GTAAIKAKFAEYGVHLEEADAAAILARVRALAVELKRPLFDKELAHIYEDYQEARKKAAVTAV
>Mature_382_residues
GGQIKIVDTTLRDGEQTAGVVFANSEKLRIAKMLDAIGVDQIEAGVPVMGGDEKKVIKEIVDAGLRASIMGWNRAVIDDV
RHSIDCGCDAVAISISTSDIHIEHKLRSTREKVIESMSRACEFAKKHNLYVSVNAEDASRTDPDYLLQFARAAREAGADR
LRFCDTVGIMDPFGTYEKIKWLIEEVGLDVEMHMHNDFGMATANTLAGIRAGAKYAGVTVVGLGERAGNAALEEVVMALK
YLENIDLKFKTEQFRELAEYVSLAARRQLPAWKAVVGSNMFAHESGIHADGALKDPRTYEVMTPEEVGLERQIVIGKHSG
TAAIKAKFAEYGVHLEEADAAAILARVRALAVELKRPLFDKELAHIYEDYQEARKKAAVTAV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=383, Percent_Identity=35.2480417754569, Blast_Score=199, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6320071, Length=375, Percent_Identity=30.9333333333333, Blast_Score=190, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6320019, Length=355, Percent_Identity=31.2676056338028, Blast_Score=188, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24582381, Length=284, Percent_Identity=24.6478873239437, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 42003; Mature: 41871

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGQIKIVDTTLRDGEQTAGVVFANSEKLRIAKMLDAIGVDQIEAGVPVMGGDEKKVIKE
CCCEEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHH
IVDAGLRASIMGWNRAVIDDVRHSIDCGCDAVAISISTSDIHIEHKLRSTREKVIESMSR
HHHCCCEEECCCCCHHHHHHHHHHHCCCCCEEEEEEECCCEEHHHHHHHHHHHHHHHHHH
ACEFAKKHNLYVSVNAEDASRTDPDYLLQFARAAREAGADRLRFCDTVGIMDPFGTYEKI
HHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHH
KWLIEEVGLDVEMHMHNDFGMATANTLAGIRAGAKYAGVTVVGLGERAGNAALEEVVMAL
HHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHH
KYLENIDLKFKTEQFRELAEYVSLAARRQLPAWKAVVGSNMFAHESGIHADGALKDPRTY
HHHHCCCCEECHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEECCCCCCCCCCCCCCCEE
EVMTPEEVGLERQIVIGKHSGTAAIKAKFAEYGVHLEEADAAAILARVRALAVELKRPLF
EEECHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHH
DKELAHIYEDYQEARKKAAVTAV
HHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GGQIKIVDTTLRDGEQTAGVVFANSEKLRIAKMLDAIGVDQIEAGVPVMGGDEKKVIKE
CCEEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHH
IVDAGLRASIMGWNRAVIDDVRHSIDCGCDAVAISISTSDIHIEHKLRSTREKVIESMSR
HHHCCCEEECCCCCHHHHHHHHHHHCCCCCEEEEEEECCCEEHHHHHHHHHHHHHHHHHH
ACEFAKKHNLYVSVNAEDASRTDPDYLLQFARAAREAGADRLRFCDTVGIMDPFGTYEKI
HHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHH
KWLIEEVGLDVEMHMHNDFGMATANTLAGIRAGAKYAGVTVVGLGERAGNAALEEVVMAL
HHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHH
KYLENIDLKFKTEQFRELAEYVSLAARRQLPAWKAVVGSNMFAHESGIHADGALKDPRTY
HHHHCCCCEECHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEECCCCCCCCCCCCCCCEE
EVMTPEEVGLERQIVIGKHSGTAAIKAKFAEYGVHLEEADAAAILARVRALAVELKRPLF
EEECHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHH
DKELAHIYEDYQEARKKAAVTAV
HHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]