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The map label for this gene is speE

Identifier: 83590650

GI number: 83590650

Start: 1875507

End: 1876358

Strand: Reverse

Name: speE

Synonym: Moth_1816

Alternate gene names: 83590650

Gene position: 1876358-1875507 (Counterclockwise)

Preceding gene: 83590651

Following gene: 83590649

Centisome position: 71.38

GC content: 57.39

Gene sequence:

>852_bases
TTGCGCGGTATTTGGTTTTCAGAACTACAGACACCGGACCTGGCGATTTCCGTTCGTCTGAACCAAACCCTGCATCATGA
AAAAACCCCCTACCAGGAGCTGGCCGTGGTGGATACGGAAGCCTACGGCCGCATGCTCCTTCTGGACAATATTATCCAGA
CGACCGTTAAGGACGAGTTCTTCTATCATGAGATGATCGCCCACGTGCCCTTGAATACCCATCCCAACCCCAGGACCGCC
CTGGTTATCGGTGGCGGCGACGGCGGCGTGGTCCGGGAAATTGTCAAACACCCTTCAATTGAGAAGGTTACCCTGGTGGA
GATTGACGCCCGGGTGGTGGCCAATGCCCGCGAGTACCTGCCGGAGATCGCCTGCGGCTTAGACGACGCCCGGGTGGAGA
TCCGCTTTGAAGACGGCATCGAGCACGTCCGCCAGCGGGAGAATACTTATGACGTAATTATCGTCGACTCTACCGACCCC
ATCGGGCCGGCAGTGGGCCTCTTCAGCGCTGAGTTTTACCGTAACGTCTACCGGGCTTTAAAAGCCGATGGTGTATTCGT
CGCCCAGACGGAATCCCCTATCTTTAACAGCAGGCTTATCCGCCGGATCCAGCGCGACCTCAAGGAGATCTTCCCCATAG
CCAGGCTATACCTGACCACGGTGCCCACCTATCCGGGTGGTCTTTGGGCCTTCAGCCTGGGGAGCAAGAAGTATGACCCT
CTGGAGGTCGATTGGAGACAGGCCCCCAAGGTTGCGACCCGTTATTATACCCCGGCCATTCACCAGGCCGCCTTTTCCCT
TCCCCCATTTGTCCAGGAGTTCCTGGAGGCCCCGGAGGAAGAAGAGTTTTAG

Upstream 100 bases:

>100_bases
CCAGGCAGTGATGGGTATTATTTAAACCCCTGGCGGAAAATAACTTGTGGCGACGGGCGATTTTTGTTAAACTACACCCT
GAAGAAAGGAGAGATTGGCT

Downstream 100 bases:

>100_bases
CAGCGGCGATTTTGGGGCGCCAGGAACGGCTCCATAGTTCGGGGAGGGAGTGTTAAATTCCCATGAATAACTCCGCTATC
TTAGAAGGCTTTACCAGGGG

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MRGIWFSELQTPDLAISVRLNQTLHHEKTPYQELAVVDTEAYGRMLLLDNIIQTTVKDEFFYHEMIAHVPLNTHPNPRTA
LVIGGGDGGVVREIVKHPSIEKVTLVEIDARVVANAREYLPEIACGLDDARVEIRFEDGIEHVRQRENTYDVIIVDSTDP
IGPAVGLFSAEFYRNVYRALKADGVFVAQTESPIFNSRLIRRIQRDLKEIFPIARLYLTTVPTYPGGLWAFSLGSKKYDP
LEVDWRQAPKVATRYYTPAIHQAAFSLPPFVQEFLEAPEEEEF

Sequences:

>Translated_283_residues
MRGIWFSELQTPDLAISVRLNQTLHHEKTPYQELAVVDTEAYGRMLLLDNIIQTTVKDEFFYHEMIAHVPLNTHPNPRTA
LVIGGGDGGVVREIVKHPSIEKVTLVEIDARVVANAREYLPEIACGLDDARVEIRFEDGIEHVRQRENTYDVIIVDSTDP
IGPAVGLFSAEFYRNVYRALKADGVFVAQTESPIFNSRLIRRIQRDLKEIFPIARLYLTTVPTYPGGLWAFSLGSKKYDP
LEVDWRQAPKVATRYYTPAIHQAAFSLPPFVQEFLEAPEEEEF
>Mature_283_residues
MRGIWFSELQTPDLAISVRLNQTLHHEKTPYQELAVVDTEAYGRMLLLDNIIQTTVKDEFFYHEMIAHVPLNTHPNPRTA
LVIGGGDGGVVREIVKHPSIEKVTLVEIDARVVANAREYLPEIACGLDDARVEIRFEDGIEHVRQRENTYDVIIVDSTDP
IGPAVGLFSAEFYRNVYRALKADGVFVAQTESPIFNSRLIRRIQRDLKEIFPIARLYLTTVPTYPGGLWAFSLGSKKYDP
LEVDWRQAPKVATRYYTPAIHQAAFSLPPFVQEFLEAPEEEEF

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=283, Percent_Identity=40.6360424028269, Blast_Score=239, Evalue=1e-63,
Organism=Escherichia coli, GI1786312, Length=265, Percent_Identity=41.1320754716981, Blast_Score=217, Evalue=5e-58,
Organism=Caenorhabditis elegans, GI212645616, Length=253, Percent_Identity=40.7114624505929, Blast_Score=202, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI25154106, Length=282, Percent_Identity=38.6524822695035, Blast_Score=200, Evalue=8e-52,
Organism=Saccharomyces cerevisiae, GI6325326, Length=279, Percent_Identity=41.5770609318996, Blast_Score=234, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6323175, Length=286, Percent_Identity=33.5664335664336, Blast_Score=185, Evalue=7e-48,
Organism=Drosophila melanogaster, GI24645443, Length=276, Percent_Identity=42.3913043478261, Blast_Score=248, Evalue=3e-66,
Organism=Drosophila melanogaster, GI21355941, Length=213, Percent_Identity=40.8450704225352, Blast_Score=183, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE_MOOTA (Q2RHH3)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_430659.1
- ProteinModelPortal:   Q2RHH3
- SMR:   Q2RHH3
- STRING:   Q2RHH3
- GeneID:   3830737
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_1816
- NMPDR:   fig|264732.9.peg.1821
- eggNOG:   COG0421
- HOGENOM:   HBG635113
- OMA:   FVAQTES
- PhylomeDB:   Q2RHH3
- BioCyc:   MTHE264732:MOTH_1816-MONOMER
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558
- TIGRFAMs:   TIGR00417

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 32226; Mature: 32226

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: BINDING 32-32 BINDING 87-87 BINDING 107-107 BINDING 156-156 BINDING 159-159

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGIWFSELQTPDLAISVRLNQTLHHEKTPYQELAVVDTEAYGRMLLLDNIIQTTVKDEF
CCCCCCCCCCCCCEEEEEEECHHHCCCCCCHHHEEEEEHHHHCHHHHHHHHHHHHHHHHH
FYHEMIAHVPLNTHPNPRTALVIGGGDGGVVREIVKHPSIEKVTLVEIDARVVANAREYL
HHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHH
PEIACGLDDARVEIRFEDGIEHVRQRENTYDVIIVDSTDPIGPAVGLFSAEFYRNVYRAL
HHHHCCCCCCEEEEEEHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
KADGVFVAQTESPIFNSRLIRRIQRDLKEIFPIARLYLTTVPTYPGGLWAFSLGSKKYDP
HCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCC
LEVDWRQAPKVATRYYTPAIHQAAFSLPPFVQEFLEAPEEEEF
CCCCHHHCCHHHHHHCCHHHHHHHHCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRGIWFSELQTPDLAISVRLNQTLHHEKTPYQELAVVDTEAYGRMLLLDNIIQTTVKDEF
CCCCCCCCCCCCCEEEEEEECHHHCCCCCCHHHEEEEEHHHHCHHHHHHHHHHHHHHHHH
FYHEMIAHVPLNTHPNPRTALVIGGGDGGVVREIVKHPSIEKVTLVEIDARVVANAREYL
HHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHH
PEIACGLDDARVEIRFEDGIEHVRQRENTYDVIIVDSTDPIGPAVGLFSAEFYRNVYRAL
HHHHCCCCCCEEEEEEHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
KADGVFVAQTESPIFNSRLIRRIQRDLKEIFPIARLYLTTVPTYPGGLWAFSLGSKKYDP
HCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCC
LEVDWRQAPKVATRYYTPAIHQAAFSLPPFVQEFLEAPEEEEF
CCCCHHHCCHHHHHHCCHHHHHHHHCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA