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The map label for this gene is pfkA2 [H]

Identifier: 83590573

GI number: 83590573

Start: 1788966

End: 1790048

Strand: Reverse

Name: pfkA2 [H]

Synonym: Moth_1738

Alternate gene names: 83590573

Gene position: 1790048-1788966 (Counterclockwise)

Preceding gene: 83590574

Following gene: 83590571

Centisome position: 68.09

GC content: 61.68

Gene sequence:

>1083_bases
TTGCGCAAACGCCTGGGTATTCTCACCGGCGGCGGCGACTGCCCCGGACTCAATGCCGTCATCAGGGCGGCGACGAAGAC
GGCCATGAGCCACGGCTATGAGATGATCGGCTTTCTGGATGGTTATACCGGCGTCGTTGAAGGCCGGTATATCAGGCTGG
ACCCAGCGGCTATTTCCGGCCTCCTCCACCGGGGAGGCACCATTCTGGGTACCAATAACCGCAGCAATCCCTTTTATTTT
CCTATCCAGGAAAATGGCGAGACCATTTACCGGGATATGTCGGACCTGGCCATCGAGCGCCTTAAGGAGATGAACATCGA
GGTTCTCCTGGTGGTTGGCGGTGATGGCACCCTGGCGGGGGCCCGGGACTTTAAGGCCAAAGGGGCGCGAGTCATCGGCA
TCCCCAAGACCATCGACAACGACCTGGCGGCCACCGACCAGACCTTCGGTTTCGATACGGCCGTCAGGACGGCCACCGAC
GCCCTGGATAAATTACATACCACGGCGGAATCCCATCACCGGGTCATGGTCCTGGAGCTTATGGGTCGTTATGCCGGCTG
GATCGCCCTGTACAGCGGCCTGGCTGGCGGGGCGGATGTAATCTTAATTCCTGAGATTCCCTGGAATATAGAAAGCGTGC
TGGCTAAAATAGAGGCCCGGCGGGCCGAGGGTAAGCCCTTCAGCATAGTCGTTGTTGCCGAAGGGGTCAAATCGCCGGAG
GGTGAGCTGGTCGTCCAGCGCCGGGTGGAAGGTAGCGTGGAAAGGGTGCGCCTGGGTGGCATCGGCCAGCTGGTGGGTAA
ATTGATAGAAGAGAAGAGCGGCATCGAAACCCGGGTGACGGTCCTGGGCCATATCCAGCGGGGCGGCTCGCCTTCCTCCT
ACGACCGCGTTCTGGCCACTCGCTTCGGGGTGGCGGCCGCCGAACTGGCCGTCAGGGGCGTCCACGGGGTCATGGTCTGC
CTGCGGGGCAGCGATATCTCCCATGTCCCCCTGGAAGAAGCCGTCGGCAAGCCCCGGACCATCCCAAGGGACCACCAGCT
CCTGGCCGCAGCCAGGGCCATTGGAATTAGCTTCGGGGAGTGA

Upstream 100 bases:

>100_bases
GCCTGGACGGAGCCGGGACCAGACTATCCGCACGTACTGTGGTTTGCTTTTCTTCCTGGAAATTGCTATGATGGTTAAAA
ATTAAAGGGGGATGAGGGAT

Downstream 100 bases:

>100_bases
TGAAAAACATCATCGCCGCTGTCCCGCCGGGTTGCCGGTGCCAGTTGCCATATGGCCGGCACCGGGCCTCAGTAATTGAG
GATAACCAGTTCGGTGATGC

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase 2; Phosphohexokinase 2 [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MRKRLGILTGGGDCPGLNAVIRAATKTAMSHGYEMIGFLDGYTGVVEGRYIRLDPAAISGLLHRGGTILGTNNRSNPFYF
PIQENGETIYRDMSDLAIERLKEMNIEVLLVVGGDGTLAGARDFKAKGARVIGIPKTIDNDLAATDQTFGFDTAVRTATD
ALDKLHTTAESHHRVMVLELMGRYAGWIALYSGLAGGADVILIPEIPWNIESVLAKIEARRAEGKPFSIVVVAEGVKSPE
GELVVQRRVEGSVERVRLGGIGQLVGKLIEEKSGIETRVTVLGHIQRGGSPSSYDRVLATRFGVAAAELAVRGVHGVMVC
LRGSDISHVPLEEAVGKPRTIPRDHQLLAAARAIGISFGE

Sequences:

>Translated_360_residues
MRKRLGILTGGGDCPGLNAVIRAATKTAMSHGYEMIGFLDGYTGVVEGRYIRLDPAAISGLLHRGGTILGTNNRSNPFYF
PIQENGETIYRDMSDLAIERLKEMNIEVLLVVGGDGTLAGARDFKAKGARVIGIPKTIDNDLAATDQTFGFDTAVRTATD
ALDKLHTTAESHHRVMVLELMGRYAGWIALYSGLAGGADVILIPEIPWNIESVLAKIEARRAEGKPFSIVVVAEGVKSPE
GELVVQRRVEGSVERVRLGGIGQLVGKLIEEKSGIETRVTVLGHIQRGGSPSSYDRVLATRFGVAAAELAVRGVHGVMVC
LRGSDISHVPLEEAVGKPRTIPRDHQLLAAARAIGISFGE
>Mature_360_residues
MRKRLGILTGGGDCPGLNAVIRAATKTAMSHGYEMIGFLDGYTGVVEGRYIRLDPAAISGLLHRGGTILGTNNRSNPFYF
PIQENGETIYRDMSDLAIERLKEMNIEVLLVVGGDGTLAGARDFKAKGARVIGIPKTIDNDLAATDQTFGFDTAVRTATD
ALDKLHTTAESHHRVMVLELMGRYAGWIALYSGLAGGADVILIPEIPWNIESVLAKIEARRAEGKPFSIVVVAEGVKSPE
GELVVQRRVEGSVERVRLGGIGQLVGKLIEEKSGIETRVTVLGHIQRGGSPSSYDRVLATRFGVAAAELAVRGVHGVMVC
LRGSDISHVPLEEAVGKPRTIPRDHQLLAAARAIGISFGE

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=391, Percent_Identity=31.9693094629156, Blast_Score=185, Evalue=7e-47,
Organism=Homo sapiens, GI48762920, Length=391, Percent_Identity=30.9462915601023, Blast_Score=178, Evalue=6e-45,
Organism=Homo sapiens, GI266453768, Length=373, Percent_Identity=31.0991957104558, Blast_Score=174, Evalue=9e-44,
Organism=Homo sapiens, GI266453748, Length=373, Percent_Identity=31.0991957104558, Blast_Score=174, Evalue=9e-44,
Organism=Homo sapiens, GI4505749, Length=373, Percent_Identity=31.0991957104558, Blast_Score=174, Evalue=9e-44,
Organism=Homo sapiens, GI266453619, Length=365, Percent_Identity=31.7808219178082, Blast_Score=174, Evalue=1e-43,
Organism=Escherichia coli, GI1790350, Length=335, Percent_Identity=38.5074626865672, Blast_Score=231, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI25147584, Length=390, Percent_Identity=31.5384615384615, Blast_Score=168, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17558788, Length=398, Percent_Identity=29.1457286432161, Blast_Score=161, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI72003601, Length=202, Percent_Identity=33.6633663366337, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321679, Length=374, Percent_Identity=31.8181818181818, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6323861, Length=389, Percent_Identity=29.8200514138817, Blast_Score=158, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24652337, Length=373, Percent_Identity=32.9758713136729, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI17647809, Length=373, Percent_Identity=32.9758713136729, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI28573326, Length=374, Percent_Identity=32.620320855615, Blast_Score=183, Evalue=1e-46,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 38525; Mature: 38525

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKRLGILTGGGDCPGLNAVIRAATKTAMSHGYEMIGFLDGYTGVVEGRYIRLDPAAISG
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCEECCCEEEECHHHHHH
LLHRGGTILGTNNRSNPFYFPIQENGETIYRDMSDLAIERLKEMNIEVLLVVGGDGTLAG
HHHCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCC
ARDFKAKGARVIGIPKTIDNDLAATDQTFGFDTAVRTATDALDKLHTTAESHHRVMVLEL
CCCHHCCCCEEEECCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEHHHH
MGRYAGWIALYSGLAGGADVILIPEIPWNIESVLAKIEARRAEGKPFSIVVVAEGVKSPE
HHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
GELVVQRRVEGSVERVRLGGIGQLVGKLIEEKSGIETRVTVLGHIQRGGSPSSYDRVLAT
CCEEEEEHHCCCCEEEEECCHHHHHHHHHHHCCCCCEEEEEEEEHHCCCCCCHHHHHHHH
RFGVAAAELAVRGVHGVMVCLRGSDISHVPLEEAVGKPRTIPRDHQLLAAARAIGISFGE
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRKRLGILTGGGDCPGLNAVIRAATKTAMSHGYEMIGFLDGYTGVVEGRYIRLDPAAISG
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCEECCCEEEECHHHHHH
LLHRGGTILGTNNRSNPFYFPIQENGETIYRDMSDLAIERLKEMNIEVLLVVGGDGTLAG
HHHCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCC
ARDFKAKGARVIGIPKTIDNDLAATDQTFGFDTAVRTATDALDKLHTTAESHHRVMVLEL
CCCHHCCCCEEEECCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEHHHH
MGRYAGWIALYSGLAGGADVILIPEIPWNIESVLAKIEARRAEGKPFSIVVVAEGVKSPE
HHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
GELVVQRRVEGSVERVRLGGIGQLVGKLIEEKSGIETRVTVLGHIQRGGSPSSYDRVLAT
CCEEEEEHHCCCCEEEEECCHHHHHHHHHHHCCCCCEEEEEEEEHHCCCCCCHHHHHHHH
RFGVAAAELAVRGVHGVMVCLRGSDISHVPLEEAVGKPRTIPRDHQLLAAARAIGISFGE
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]