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The map label for this gene is rluB [H]

Identifier: 83590199

GI number: 83590199

Start: 1398101

End: 1398823

Strand: Reverse

Name: rluB [H]

Synonym: Moth_1352

Alternate gene names: 83590199

Gene position: 1398823-1398101 (Counterclockwise)

Preceding gene: 83590202

Following gene: 83590198

Centisome position: 53.21

GC content: 59.89

Gene sequence:

>723_bases
ATGCGCTTGCAGAAGTACCTGGCCCTGGCCGGGGTGGCTTCCCGGCGCCGGGCCGAAGACCTTATCCGTGCCGGGCGGGT
GCGGGTAAACGGCCGGGTCATAAAAGTTATGGGTGTCCAGGTGGAACCGGGGCAGGATAAAGTGACTGTCGACGGCCAGC
CTGTGACCGGGCCGGAGAGAAAGTATTACCTTCTCCTGTTTAAACCGGCCGGTTATGTAACTACCGTCTCCGATCCCCGG
GGACGGCCCAAGGTCACTGACCTGGTGCACGGCATACCTGCTAGATTATATCCCGTGGGCCGCCTGGATTATGCCACCGA
GGGTCTTCTCTTGCTAACCAACGACGGCGAGCTAACCCTGCGCCTGACCCACCCCAGATACGGGGTAACGAAGACCTACC
TGGCTCTGGTCAGGGGTATACCCGATGCTAATACTATCCAGCATTTACGCTGCGGTGTCAAGCTGGAAGACGGCCCTACA
GCTCCTGCGAGGGTAAATTTGTGCCGGGCGGGGAAAGATAGCGCCCTCCTGGAATTAACCCTCAGGGAAGGCCGGAACCG
GGAGGTGCGACGTATGCTGGCGGCGGTCGGTCACCCGGTCCTGCGCCTCCGGCGGATCCGCCTGGCCTTTTTAACCCTTC
GAGGTTTAAAACCCGGCACCTATCGCCACCTCACCCCCCACGAAGTCGAACGATTGTACCGGCTGGTAGGTTTAAAAAAA
TAG

Upstream 100 bases:

>100_bases
GATCACCGGTTTGAAGCCCCTCTTTTCTATTCATTTTGAAGTTCGCCAGCAAATAAGCAGGTATCGGAGAGCAGGACTTT
TTTCCCTTCGGAGGATGATT

Downstream 100 bases:

>100_bases
CCCCTGGCCTGTTTTTCCTCGCCAAAGCCATCCAGGCATGATAGAATGGTGATAAATGCTGATAGGAGGCAATGGTAAAA
TTGGCTGGTTTAGAACAGGG

Product: ribosomal large subunit pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MRLQKYLALAGVASRRRAEDLIRAGRVRVNGRVIKVMGVQVEPGQDKVTVDGQPVTGPERKYYLLLFKPAGYVTTVSDPR
GRPKVTDLVHGIPARLYPVGRLDYATEGLLLLTNDGELTLRLTHPRYGVTKTYLALVRGIPDANTIQHLRCGVKLEDGPT
APARVNLCRAGKDSALLELTLREGRNREVRRMLAAVGHPVLRLRRIRLAFLTLRGLKPGTYRHLTPHEVERLYRLVGLKK

Sequences:

>Translated_240_residues
MRLQKYLALAGVASRRRAEDLIRAGRVRVNGRVIKVMGVQVEPGQDKVTVDGQPVTGPERKYYLLLFKPAGYVTTVSDPR
GRPKVTDLVHGIPARLYPVGRLDYATEGLLLLTNDGELTLRLTHPRYGVTKTYLALVRGIPDANTIQHLRCGVKLEDGPT
APARVNLCRAGKDSALLELTLREGRNREVRRMLAAVGHPVLRLRRIRLAFLTLRGLKPGTYRHLTPHEVERLYRLVGLKK
>Mature_240_residues
MRLQKYLALAGVASRRRAEDLIRAGRVRVNGRVIKVMGVQVEPGQDKVTVDGQPVTGPERKYYLLLFKPAGYVTTVSDPR
GRPKVTDLVHGIPARLYPVGRLDYATEGLLLLTNDGELTLRLTHPRYGVTKTYLALVRGIPDANTIQHLRCGVKLEDGPT
APARVNLCRAGKDSALLELTLREGRNREVRRMLAAVGHPVLRLRRIRLAFLTLRGLKPGTYRHLTPHEVERLYRLVGLKK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=244, Percent_Identity=41.8032786885246, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1788510, Length=232, Percent_Identity=36.2068965517241, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1790453, Length=238, Percent_Identity=32.7731092436975, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI87081838, Length=189, Percent_Identity=38.6243386243386, Blast_Score=108, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 26888; Mature: 26888

Theoretical pI: Translated: 11.26; Mature: 11.26

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLQKYLALAGVASRRRAEDLIRAGRVRVNGRVIKVMGVQVEPGQDKVTVDGQPVTGPER
CCHHHHHHHHHHHHHHHHHHHHHHCEEEECCEEEEEEEEEECCCCCEEEECCCCCCCCCC
KYYLLLFKPAGYVTTVSDPRGRPKVTDLVHGIPARLYPVGRLDYATEGLLLLTNDGELTL
EEEEEEECCCCEEEECCCCCCCCHHHHHHCCCCHHEEECCCCCCCCCCEEEEECCCEEEE
RLTHPRYGVTKTYLALVRGIPDANTIQHLRCGVKLEDGPTAPARVNLCRAGKDSALLELT
EEECCCCCHHHHHHHHHHCCCCCCHHHHHHCCEEECCCCCCCCEEEEECCCCCCEEEEEE
LREGRNREVRRMLAAVGHPVLRLRRIRLAFLTLRGLKPGTYRHLTPHEVERLYRLVGLKK
ECCCCCHHHHHHHHHHCCHHHHHHHHHHEEHHHCCCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRLQKYLALAGVASRRRAEDLIRAGRVRVNGRVIKVMGVQVEPGQDKVTVDGQPVTGPER
CCHHHHHHHHHHHHHHHHHHHHHHCEEEECCEEEEEEEEEECCCCCEEEECCCCCCCCCC
KYYLLLFKPAGYVTTVSDPRGRPKVTDLVHGIPARLYPVGRLDYATEGLLLLTNDGELTL
EEEEEEECCCCEEEECCCCCCCCHHHHHHCCCCHHEEECCCCCCCCCCEEEEECCCEEEE
RLTHPRYGVTKTYLALVRGIPDANTIQHLRCGVKLEDGPTAPARVNLCRAGKDSALLELT
EEECCCCCHHHHHHHHHHCCCCCCHHHHHHCCEEECCCCCCCCEEEEECCCCCCEEEEEE
LREGRNREVRRMLAAVGHPVLRLRRIRLAFLTLRGLKPGTYRHLTPHEVERLYRLVGLKK
ECCCCCHHHHHHHHHHCCHHHHHHHHHHEEHHHCCCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]