Click here to switch to the map view.

The map label for this gene is metH [H]

Identifier: 83590165

GI number: 83590165

Start: 1362693

End: 1363490

Strand: Reverse

Name: metH [H]

Synonym: Moth_1318

Alternate gene names: 83590165

Gene position: 1363490-1362693 (Counterclockwise)

Preceding gene: 83590166

Following gene: 83590164

Centisome position: 51.87

GC content: 47.99

Gene sequence:

>798_bases
ATGTTAATCATCGGTGAAAAACTAAATAGCGCGATCCCCAGTGTCCGTCAAATCATTAATGACAAAAACGCCGCGGCCGT
GCAGGACCTGGCCCGCAGGCAGGCGGAAGCGGGAGCAGATTACCTGGATCTAAATACCGCCCACTGTGACGAAGTGGCCG
ATATGGAGTGGCTTGTGCATACAGTGCAAGAAGTAACTGATGTACCTTTATGCATTGATAGCACAGCAGGGGAGGCAATA
AAAAAGGGTCTGGAGACCGTCAAGGGCGATAAGAGCAAGGTGATAATCAACTCCATATCCATGGAAAAAAAGCGGCTGGA
AGAAGTATTGCCACTTGTACTCGAATACCAGTGCCCGGTAATCGGCCTGACGGTTGACGACAATGGTATCCCGAAAACGG
CTGAAGAAAGGATCAAGATAACTGAGGGACTGATAGAGACCCTGAGCAGGAAGAATTATGATTTGAGCAACCTGTATATC
GATCCGCTGGTTCTCCCCCTGGCCGTAAACCACACCAACGCGGTGATGTTTTTCCAGTGCCTGAGGGAAATCAAGAGGTT
ATTTAAAGTGAAGACAGTATCCGGTTTAAGCAACATCTCGTTTAACATGCCGAAGAGGAAGCTCATCAACCGTTACTTCC
TGACAATATGTATGGCGAGCGGCATGGACGCGGCCATTCTCGATCCCTTGGATGGAAAAATCATGACTGCAGTCACCACC
ACCGATTTACTGCTGGGTAACGACCGTTTCGGCAAGAACTTTTTAAAGGCATATCGTAAAGATTTGTTAGCGGATTAA

Upstream 100 bases:

>100_bases
CTTTAAACGGTTCTATTGCAAAAAACCTTGGGTAGCTATCCTGCATGAGGAATAGTTTCCGTAGAAACGCCTGAAACCAA
ATACATGGAGGGTACAGGAA

Downstream 100 bases:

>100_bases
AGTAATACAAATTTAGGGGGTGAAGTAATACGGTTGAGCAGGAGAAAGGAAAGCAAGAACTGATGTAGTGCATTATTAGA
GGGACGGTATATAAGTAAAA

Product: methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MLIIGEKLNSAIPSVRQIINDKNAAAVQDLARRQAEAGADYLDLNTAHCDEVADMEWLVHTVQEVTDVPLCIDSTAGEAI
KKGLETVKGDKSKVIINSISMEKKRLEEVLPLVLEYQCPVIGLTVDDNGIPKTAEERIKITEGLIETLSRKNYDLSNLYI
DPLVLPLAVNHTNAVMFFQCLREIKRLFKVKTVSGLSNISFNMPKRKLINRYFLTICMASGMDAAILDPLDGKIMTAVTT
TDLLLGNDRFGKNFLKAYRKDLLAD

Sequences:

>Translated_265_residues
MLIIGEKLNSAIPSVRQIINDKNAAAVQDLARRQAEAGADYLDLNTAHCDEVADMEWLVHTVQEVTDVPLCIDSTAGEAI
KKGLETVKGDKSKVIINSISMEKKRLEEVLPLVLEYQCPVIGLTVDDNGIPKTAEERIKITEGLIETLSRKNYDLSNLYI
DPLVLPLAVNHTNAVMFFQCLREIKRLFKVKTVSGLSNISFNMPKRKLINRYFLTICMASGMDAAILDPLDGKIMTAVTT
TDLLLGNDRFGKNFLKAYRKDLLAD
>Mature_265_residues
MLIIGEKLNSAIPSVRQIINDKNAAAVQDLARRQAEAGADYLDLNTAHCDEVADMEWLVHTVQEVTDVPLCIDSTAGEAI
KKGLETVKGDKSKVIINSISMEKKRLEEVLPLVLEYQCPVIGLTVDDNGIPKTAEERIKITEGLIETLSRKNYDLSNLYI
DPLVLPLAVNHTNAVMFFQCLREIKRLFKVKTVSGLSNISFNMPKRKLINRYFLTICMASGMDAAILDPLDGKIMTAVTT
TDLLLGNDRFGKNFLKAYRKDLLAD

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790450, Length=244, Percent_Identity=28.2786885245902, Blast_Score=78, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17535405, Length=246, Percent_Identity=28.0487804878049, Blast_Score=78, Evalue=5e-15,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 29483; Mature: 29483

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIIGEKLNSAIPSVRQIINDKNAAAVQDLARRQAEAGADYLDLNTAHCDEVADMEWLVH
CEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHH
TVQEVTDVPLCIDSTAGEAIKKGLETVKGDKSKVIINSISMEKKRLEEVLPLVLEYQCPV
HHHHHHCCCEEECCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCE
IGLTVDDNGIPKTAEERIKITEGLIETLSRKNYDLSNLYIDPLVLPLAVNHTNAVMFFQC
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHEEECHHHEEEEECCCHHHHHHHH
LREIKRLFKVKTVSGLSNISFNMPKRKLINRYFLTICMASGMDAAILDPLDGKIMTAVTT
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEH
TDLLLGNDRFGKNFLKAYRKDLLAD
HHHEECCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MLIIGEKLNSAIPSVRQIINDKNAAAVQDLARRQAEAGADYLDLNTAHCDEVADMEWLVH
CEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHH
TVQEVTDVPLCIDSTAGEAIKKGLETVKGDKSKVIINSISMEKKRLEEVLPLVLEYQCPV
HHHHHHCCCEEECCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCE
IGLTVDDNGIPKTAEERIKITEGLIETLSRKNYDLSNLYIDPLVLPLAVNHTNAVMFFQC
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHEEECHHHEEEEECCCHHHHHHHH
LREIKRLFKVKTVSGLSNISFNMPKRKLINRYFLTICMASGMDAAILDPLDGKIMTAVTT
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEH
TDLLLGNDRFGKNFLKAYRKDLLAD
HHHEECCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]