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The map label for this gene is mnmE [H]

Identifier: 83590125

GI number: 83590125

Start: 1319448

End: 1320665

Strand: Reverse

Name: mnmE [H]

Synonym: Moth_1277

Alternate gene names: 83590125

Gene position: 1320665-1319448 (Counterclockwise)

Preceding gene: 83590126

Following gene: 83590124

Centisome position: 50.24

GC content: 59.44

Gene sequence:

>1218_bases
ATGGACACAACGCCGCGGGGCAACCGCCTGCATATAGCCATCTTCGGCCGCCGTAACGCAGGTAAGTCCAGCCTGATCAA
TGCCCTGACCAACCAGGACCTGGCGGTGGTCTCCAGCGTACCCGGGACGACCACCGACCCGGTCATGAAATCCATGGAGA
TCCTGCCCCTGGGGCCAGTGGTCCTAATCGATACGGCCGGTATTGACGACGTGGGGGAACTGGGCACCCTGAGGGTCAAG
AAGACCATGGAAGTATTACGCCGCACCGACCTGGCCATTCTGGTCCTGGACCCGGCCGCCGGAGCCGGACCTTACGAGCT
GGAGCTGCAACAGCGTTTAAAGGAAGAAAGGCTGCCCCTGGTGATAGTCTTAAATAAAATCGATCAGGGCGGTATGGAAC
TTCTCCCCGGCCTGGAGAAAACCCTGGGGCAACGGGTCCTGCCAGTGAGCGCCCTGACCCGCCAGGGTATAGAAGAATTA
AAGGGAGAGCTCATCAAGGCCGCCCCGGCCGACTTCGAGGAACCGTACATCGTTGCCGATCTAGTGGGCCCAGGGGAAAT
GGCCGTCCTGGTGGCGCCCATCGACCTGGCGGCACCCAAAGGTCGCTTGATCCTGCCCCAGGTTCAGACCCTGCGGGACC
TGCTGGACCACGATGCTATGGGCCTGGTGGTCAAAGAGCGGGAACTAAAGCTCGCCCTGGCCAAGATCCCACCCCCGAAG
ATTGTCATCACCGATTCTCAGGCCTTTCTCAAGGTAGCTGCCGACACGCCCCCCGGCGTCTGGATGACCTCCTTCTCCAT
CCTCTTCGCCCGCTATAAAGGGGATCTGGTAGAATTGGTTCGTGGTGCCAGGGCGGTAAAAAATTTACAGCCAGGGGATA
AAGTCCTGATTGCCGAGGCCTGCACCCACCACCGCCAGGCTGATGACATCGGCAAGGTGCAGATACCCCGCTGGTTGCGC
CAGTATGTTGGCGGTGACCTGCAGTTCAGCTGGTATTCCGGGAGCGGTTTTCCCGAGGATGTGGCCAGCTATAAAGTTAT
TATCCATTGCGGCGGCTGTATGATCAATCGCCGGGAAATGCTCTATCGCATCCAGCTGGCTAAAGAAGCCGGGGTTCCCA
TTGTGAACTATGGCGTCCTCTTGGCCTTCGTCCACGGTGTCCTGGAACGGGCCCTGGAACCCTTCCCCCTGGCCTTGATG
GAATGGCTGGGGGAATAA

Upstream 100 bases:

>100_bases
AATCGACGACGGTAACAACAACTCTGATAAAGAGAAAGCAACGAGACAGGCCAGTTAAAACGGGAGTTAAGCTCCCGTTT
TTTTTGAGAGGGTGAGCTTG

Downstream 100 bases:

>100_bases
ATATCGATCAGGTGATCATTGATGCCAGGTGAGCCAAAGAACCCTGTCCGGGCGCGTTACTGGCGAGTTAACGGCAAACA
GGGATATGATCTGCAGGTTT

Product: small GTP-binding protein domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MDTTPRGNRLHIAIFGRRNAGKSSLINALTNQDLAVVSSVPGTTTDPVMKSMEILPLGPVVLIDTAGIDDVGELGTLRVK
KTMEVLRRTDLAILVLDPAAGAGPYELELQQRLKEERLPLVIVLNKIDQGGMELLPGLEKTLGQRVLPVSALTRQGIEEL
KGELIKAAPADFEEPYIVADLVGPGEMAVLVAPIDLAAPKGRLILPQVQTLRDLLDHDAMGLVVKERELKLALAKIPPPK
IVITDSQAFLKVAADTPPGVWMTSFSILFARYKGDLVELVRGARAVKNLQPGDKVLIAEACTHHRQADDIGKVQIPRWLR
QYVGGDLQFSWYSGSGFPEDVASYKVIIHCGGCMINRREMLYRIQLAKEAGVPIVNYGVLLAFVHGVLERALEPFPLALM
EWLGE

Sequences:

>Translated_405_residues
MDTTPRGNRLHIAIFGRRNAGKSSLINALTNQDLAVVSSVPGTTTDPVMKSMEILPLGPVVLIDTAGIDDVGELGTLRVK
KTMEVLRRTDLAILVLDPAAGAGPYELELQQRLKEERLPLVIVLNKIDQGGMELLPGLEKTLGQRVLPVSALTRQGIEEL
KGELIKAAPADFEEPYIVADLVGPGEMAVLVAPIDLAAPKGRLILPQVQTLRDLLDHDAMGLVVKERELKLALAKIPPPK
IVITDSQAFLKVAADTPPGVWMTSFSILFARYKGDLVELVRGARAVKNLQPGDKVLIAEACTHHRQADDIGKVQIPRWLR
QYVGGDLQFSWYSGSGFPEDVASYKVIIHCGGCMINRREMLYRIQLAKEAGVPIVNYGVLLAFVHGVLERALEPFPLALM
EWLGE
>Mature_405_residues
MDTTPRGNRLHIAIFGRRNAGKSSLINALTNQDLAVVSSVPGTTTDPVMKSMEILPLGPVVLIDTAGIDDVGELGTLRVK
KTMEVLRRTDLAILVLDPAAGAGPYELELQQRLKEERLPLVIVLNKIDQGGMELLPGLEKTLGQRVLPVSALTRQGIEEL
KGELIKAAPADFEEPYIVADLVGPGEMAVLVAPIDLAAPKGRLILPQVQTLRDLLDHDAMGLVVKERELKLALAKIPPPK
IVITDSQAFLKVAADTPPGVWMTSFSILFARYKGDLVELVRGARAVKNLQPGDKVLIAEACTHHRQADDIGKVQIPRWLR
QYVGGDLQFSWYSGSGFPEDVASYKVIIHCGGCMINRREMLYRIQLAKEAGVPIVNYGVLLAFVHGVLERALEPFPLALM
EWLGE

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG1160

COG function: function code R; Predicted GTPases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Escherichia coli, GI2367268, Length=167, Percent_Identity=30.5389221556886, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI87082120, Length=171, Percent_Identity=32.1637426900585, Blast_Score=65, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323665, Length=180, Percent_Identity=32.2222222222222, Blast_Score=74, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 44224; Mature: 44224

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTTPRGNRLHIAIFGRRNAGKSSLINALTNQDLAVVSSVPGTTTDPVMKSMEILPLGPV
CCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCCEECCCCE
VLIDTAGIDDVGELGTLRVKKTMEVLRRTDLAILVLDPAAGAGPYELELQQRLKEERLPL
EEEECCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCE
VIVLNKIDQGGMELLPGLEKTLGQRVLPVSALTRQGIEELKGELIKAAPADFEEPYIVAD
EEEEECCCCCCHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
LVGPGEMAVLVAPIDLAAPKGRLILPQVQTLRDLLDHDAMGLVVKERELKLALAKIPPPK
ECCCCCEEEEEEEEECCCCCCCEECCHHHHHHHHHCCCCCEEEEECCHHEEEEECCCCCE
IVITDSQAFLKVAADTPPGVWMTSFSILFARYKGDLVELVRGARAVKNLQPGDKVLIAEA
EEEECCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEHH
CTHHRQADDIGKVQIPRWLRQYVGGDLQFSWYSGSGFPEDVASYKVIIHCGGCMINRREM
HHCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHCEEEEEEECCCEEEHHHH
LYRIQLAKEAGVPIVNYGVLLAFVHGVLERALEPFPLALMEWLGE
HHHHEEHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHCC
>Mature Secondary Structure
MDTTPRGNRLHIAIFGRRNAGKSSLINALTNQDLAVVSSVPGTTTDPVMKSMEILPLGPV
CCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCCEECCCCE
VLIDTAGIDDVGELGTLRVKKTMEVLRRTDLAILVLDPAAGAGPYELELQQRLKEERLPL
EEEECCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCE
VIVLNKIDQGGMELLPGLEKTLGQRVLPVSALTRQGIEELKGELIKAAPADFEEPYIVAD
EEEEECCCCCCHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
LVGPGEMAVLVAPIDLAAPKGRLILPQVQTLRDLLDHDAMGLVVKERELKLALAKIPPPK
ECCCCCEEEEEEEEECCCCCCCEECCHHHHHHHHHCCCCCEEEEECCHHEEEEECCCCCE
IVITDSQAFLKVAADTPPGVWMTSFSILFARYKGDLVELVRGARAVKNLQPGDKVLIAEA
EEEECCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEHH
CTHHRQADDIGKVQIPRWLRQYVGGDLQFSWYSGSGFPEDVASYKVIIHCGGCMINRREM
HHCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHCEEEEEEECCCEEEHHHH
LYRIQLAKEAGVPIVNYGVLLAFVHGVLERALEPFPLALMEWLGE
HHHHEEHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]