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The map label for this gene is degA [H]

Identifier: 83590110

GI number: 83590110

Start: 1306526

End: 1307569

Strand: Reverse

Name: degA [H]

Synonym: Moth_1262

Alternate gene names: 83590110

Gene position: 1307569-1306526 (Counterclockwise)

Preceding gene: 83590111

Following gene: 83590109

Centisome position: 49.74

GC content: 44.83

Gene sequence:

>1044_bases
ATGGAACCGTTACCAAGAGGAATGAAAAATATGCGCATAACCATCAAAGATATAGCCCAAAGAAGCGGGGTATCCTGTTC
TACGGTTTCACGTGTTTTAACAAACCACCCCAACGTTGATCCCAAAACCAGGGAACGGGTCAAGCAAGTTATAGATGAAC
TTGGTTACCGCCCCAGTCGCATTGCCCGCGGTCTGGTTATGGGCCAAATTAACGTAGTTGCCCTTATAATTGGCGATATT
CGTAACCCTTTCTATGCAGAACTAACCCGTGCCGTAAAAGATATCCTGAATAAAGAAGGTTATATGGTTGTAGTTAGCGA
TAGTGATTATGACCCGCAAAAAGAGGAGATATATATTCGGGCAGCCGAGGAATATGGCTTTGCTGGCATTATCATGATTA
CAGCCATGGAAACCGAGGCTTTGATCCAACAGTTAGAAAAATTACGCTGCCCGGTTGTTTTGCTCAATCGCTACCTACCC
TCCGTCGAGACAGATGTTATCTCCGTAGATAATTATCTAGGCGGTTACCTGGCCGCTGAGCACCTCATCAAGCTAGGGCA
TCGTAATATCGCTCACCTGGCTGGTTTTAAAAACTCCAGTGCTACCCGAGATCGCCTTCGTGGCTTCATCGACGCCCATG
TCCATTATGGTATCCAGTTAAATCAAGAAAGAATAGTTTATGGTAATTTGCAGATGGAAGCAGGGTATAAATTCGCTAAA
GAATATTTAAGCCAGAACGAAGACATTACTGCAGTCTTTTGTGGAAACGATCTCATGGCCCTGGGATTGATAGAAGCCCT
GTACGAGGAGGGGAAAGAAATACCCCGAGATATAAGTGTTATAGGTTACGATGATATTGACATGGCTTCTCTGGCAAGGG
TAAAACTCACGACCATCCGCCAGCCCCAGTATGAAATGGGCCAGACAGCTGCCGAGGTCTTAATTGACAGAATGAAAGGT
AAAATAGGAGCACCAAAACGTATTATCTTTACACCTAAGCTGATCATCCGTGAGAGTACCGCCGAGTATAAACCCGGTAA
ATAA

Upstream 100 bases:

>100_bases
AGCCCATGTTATGTTAAGCCCCTGTTATGGGGCTTAAAATTTTATTAAGTTTTTATTAAAAAACATCTTGACAGTCTCTA
GACTGGGAGTTAATATTATC

Downstream 100 bases:

>100_bases
TAAAGTATATTTATCTATCATGGAGGGGACATATTATGGCGGTTTCGGGTTATGGTAAATGGTTCGAGTTGGCTGAAAAG
GATTTAAAAACACGCAAGTT

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Degradation activator [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MEPLPRGMKNMRITIKDIAQRSGVSCSTVSRVLTNHPNVDPKTRERVKQVIDELGYRPSRIARGLVMGQINVVALIIGDI
RNPFYAELTRAVKDILNKEGYMVVVSDSDYDPQKEEIYIRAAEEYGFAGIIMITAMETEALIQQLEKLRCPVVLLNRYLP
SVETDVISVDNYLGGYLAAEHLIKLGHRNIAHLAGFKNSSATRDRLRGFIDAHVHYGIQLNQERIVYGNLQMEAGYKFAK
EYLSQNEDITAVFCGNDLMALGLIEALYEEGKEIPRDISVIGYDDIDMASLARVKLTTIRQPQYEMGQTAAEVLIDRMKG
KIGAPKRIIFTPKLIIRESTAEYKPGK

Sequences:

>Translated_347_residues
MEPLPRGMKNMRITIKDIAQRSGVSCSTVSRVLTNHPNVDPKTRERVKQVIDELGYRPSRIARGLVMGQINVVALIIGDI
RNPFYAELTRAVKDILNKEGYMVVVSDSDYDPQKEEIYIRAAEEYGFAGIIMITAMETEALIQQLEKLRCPVVLLNRYLP
SVETDVISVDNYLGGYLAAEHLIKLGHRNIAHLAGFKNSSATRDRLRGFIDAHVHYGIQLNQERIVYGNLQMEAGYKFAK
EYLSQNEDITAVFCGNDLMALGLIEALYEEGKEIPRDISVIGYDDIDMASLARVKLTTIRQPQYEMGQTAAEVLIDRMKG
KIGAPKRIIFTPKLIIRESTAEYKPGK
>Mature_347_residues
MEPLPRGMKNMRITIKDIAQRSGVSCSTVSRVLTNHPNVDPKTRERVKQVIDELGYRPSRIARGLVMGQINVVALIIGDI
RNPFYAELTRAVKDILNKEGYMVVVSDSDYDPQKEEIYIRAAEEYGFAGIIMITAMETEALIQQLEKLRCPVVLLNRYLP
SVETDVISVDNYLGGYLAAEHLIKLGHRNIAHLAGFKNSSATRDRLRGFIDAHVHYGIQLNQERIVYGNLQMEAGYKFAK
EYLSQNEDITAVFCGNDLMALGLIEALYEEGKEIPRDISVIGYDDIDMASLARVKLTTIRQPQYEMGQTAAEVLIDRMKG
KIGAPKRIIFTPKLIIRESTAEYKPGK

Specific function: Involved in the control of degradation of B.subtilis amidophosphoribosyltransferase (purF). Probably activates the gene for a degradative protease [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=329, Percent_Identity=32.8267477203647, Blast_Score=197, Evalue=8e-52,
Organism=Escherichia coli, GI1787948, Length=331, Percent_Identity=33.8368580060423, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1789202, Length=328, Percent_Identity=32.6219512195122, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1790194, Length=329, Percent_Identity=33.7386018237082, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI1788474, Length=333, Percent_Identity=31.8318318318318, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI1789068, Length=335, Percent_Identity=29.5522388059701, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1787580, Length=341, Percent_Identity=30.4985337243402, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1787906, Length=324, Percent_Identity=30.8641975308642, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI48994940, Length=320, Percent_Identity=25.3125, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1786540, Length=315, Percent_Identity=31.1111111111111, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1790715, Length=319, Percent_Identity=25.3918495297806, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1790689, Length=342, Percent_Identity=27.7777777777778, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1789456, Length=348, Percent_Identity=25.5747126436782, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790192, Length=223, Percent_Identity=28.6995515695067, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1786268, Length=309, Percent_Identity=22.3300970873786, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 39067; Mature: 39067

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPLPRGMKNMRITIKDIAQRSGVSCSTVSRVLTNHPNVDPKTRERVKQVIDELGYRPSR
CCCCCCCHHHCEEHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHH
IARGLVMGQINVVALIIGDIRNPFYAELTRAVKDILNKEGYMVVVSDSDYDPQKEEIYIR
HHHHHHHCCHHEEEEEEHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEE
AAEEYGFAGIIMITAMETEALIQQLEKLRCPVVLLNRYLPSVETDVISVDNYLGGYLAAE
EHHHCCCEEEEEEEECCHHHHHHHHHHHCCHHHHHHHHCCCCCHHEEEHHHHHHHHHHHH
HLIKLGHRNIAHLAGFKNSSATRDRLRGFIDAHVHYGIQLNQERIVYGNLQMEAGYKFAK
HHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHEEEEEEECCCEEEEEEEEEHHHHHHHH
EYLSQNEDITAVFCGNDLMALGLIEALYEEGKEIPRDISVIGYDDIDMASLARVKLTTIR
HHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHEECCC
QPQYEMGQTAAEVLIDRMKGKIGAPKRIIFTPKLIIRESTAEYKPGK
CCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEECCCCCCCCCC
>Mature Secondary Structure
MEPLPRGMKNMRITIKDIAQRSGVSCSTVSRVLTNHPNVDPKTRERVKQVIDELGYRPSR
CCCCCCCHHHCEEHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHH
IARGLVMGQINVVALIIGDIRNPFYAELTRAVKDILNKEGYMVVVSDSDYDPQKEEIYIR
HHHHHHHCCHHEEEEEEHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEE
AAEEYGFAGIIMITAMETEALIQQLEKLRCPVVLLNRYLPSVETDVISVDNYLGGYLAAE
EHHHCCCEEEEEEEECCHHHHHHHHHHHCCHHHHHHHHCCCCCHHEEEHHHHHHHHHHHH
HLIKLGHRNIAHLAGFKNSSATRDRLRGFIDAHVHYGIQLNQERIVYGNLQMEAGYKFAK
HHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHEEEEEEECCCEEEEEEEEEHHHHHHHH
EYLSQNEDITAVFCGNDLMALGLIEALYEEGKEIPRDISVIGYDDIDMASLARVKLTTIR
HHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHEECCC
QPQYEMGQTAAEVLIDRMKGKIGAPKRIIFTPKLIIRESTAEYKPGK
CCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407808; 9353932; 9384377 [H]