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The map label for this gene is mttC2 [H]

Identifier: 83590054

GI number: 83590054

Start: 1244884

End: 1245558

Strand: Reverse

Name: mttC2 [H]

Synonym: Moth_1206

Alternate gene names: 83590054

Gene position: 1245558-1244884 (Counterclockwise)

Preceding gene: 83590058

Following gene: 83590052

Centisome position: 47.38

GC content: 56.0

Gene sequence:

>675_bases
TTGAACGGCCGTACCCAGATATTTCAGGACATTATTTCCGCCATCCTGAGGGGAGAGGCCAATGGTGTATTGAAATCAAT
CAATGCAGGGCTGCAGATGGGAATGAGTGCTGACGAGATCCTGGAAGAGGCCATGATACCGGCAACCATCCAGACCCTGG
ACAAATACCAGGGAGCTGATTTCTACATCCCCGATGTAATCAACGCCTCCCACGCCATTGAGGCCGGACTGTTCGCCTTA
AAACCTTACACCAAAAAAAGCCTCCAGAAGCGAAAGAAAATCGTTATCGGTACTGTGGAGGGGGATATTCACGACATCGG
GAAAAACCTGGTGGCCCTGTCCCTGGGTTATGCCGGGTTTGAGGTCGTCGACCTTGGTGTGAATGTCTCGCCGGCGGAAT
TTGTCAGGGCGGTAGAGCAGCACCGCCCGGAGATTCTGGCAATGTCGGCACTACTGACCACAACCATGGGCGAAATGGCC
AGGGTAATCAAGGCCCTGGAGAAGCATCACCTGCGCAAGAGCGTCAGGGTGCTGGTGGGGGGAGGGCCTGTGACGAAGGA
GTTTGCTGAGTCCATCGGGGCGGACGCCTACGGCAGCTCGGCCCGTGAGGCCATCAGTATCGCCCAATCCCTGGTGAGGC
GGTGCAGTGACCGCGAAAGGGATTATGCCTGTTGA

Upstream 100 bases:

>100_bases
CAGGTAGGAAGGTTCGGCCTCGCCGGAGGGAGTCCCCTTTAGTATACCGCAACCAACCGGGACGTTTTACGAAGGAATAA
ACAGGAAAGCCGGTGATACC

Downstream 100 bases:

>100_bases
AAGGGTAAGAACGACTGCCCGGGCGATGTTCACCCGGGCAGTGTTATATTCAGACCAATGGAGAGAAATTCTTTCTCCTA
TACTCGTTAGGACTCAAGCC

Product: cobalamin B12-binding

Products: L-Methionine; 5,6,7,8-Tetrahydrofolate [C]

Alternate protein names: TCP 2 [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MNGRTQIFQDIISAILRGEANGVLKSINAGLQMGMSADEILEEAMIPATIQTLDKYQGADFYIPDVINASHAIEAGLFAL
KPYTKKSLQKRKKIVIGTVEGDIHDIGKNLVALSLGYAGFEVVDLGVNVSPAEFVRAVEQHRPEILAMSALLTTTMGEMA
RVIKALEKHHLRKSVRVLVGGGPVTKEFAESIGADAYGSSAREAISIAQSLVRRCSDRERDYAC

Sequences:

>Translated_224_residues
MNGRTQIFQDIISAILRGEANGVLKSINAGLQMGMSADEILEEAMIPATIQTLDKYQGADFYIPDVINASHAIEAGLFAL
KPYTKKSLQKRKKIVIGTVEGDIHDIGKNLVALSLGYAGFEVVDLGVNVSPAEFVRAVEQHRPEILAMSALLTTTMGEMA
RVIKALEKHHLRKSVRVLVGGGPVTKEFAESIGADAYGSSAREAISIAQSLVRRCSDRERDYAC
>Mature_224_residues
MNGRTQIFQDIISAILRGEANGVLKSINAGLQMGMSADEILEEAMIPATIQTLDKYQGADFYIPDVINASHAIEAGLFAL
KPYTKKSLQKRKKIVIGTVEGDIHDIGKNLVALSLGYAGFEVVDLGVNVSPAEFVRAVEQHRPEILAMSALLTTTMGEMA
RVIKALEKHHLRKSVRVLVGGGPVTKEFAESIGADAYGSSAREAISIAQSLVRRCSDRERDYAC

Specific function: Acts probably as a methyl group carrier between mttB and either mtbA or mtaA [H]

COG id: COG5012

COG function: function code R; Predicted cobalamin binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=173, Percent_Identity=27.7456647398844, Blast_Score=81, Evalue=6e-16,
Organism=Escherichia coli, GI1790450, Length=192, Percent_Identity=27.0833333333333, Blast_Score=85, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17535405, Length=139, Percent_Identity=29.4964028776978, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR003759
- InterPro:   IPR012741 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2 [H]

EC number: 2.1.1.13 [C]

Molecular weight: Translated: 24258; Mature: 24258

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGRTQIFQDIISAILRGEANGVLKSINAGLQMGMSADEILEEAMIPATIQTLDKYQGAD
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHCCCC
FYIPDVINASHAIEAGLFALKPYTKKSLQKRKKIVIGTVEGDIHDIGKNLVALSLGYAGF
EECCCHHCHHHHHHHHHHHCCCHHHHHHHHHHCEEEEECCCHHHHHHHHHHHHHHCCCCE
EVVDLGVNVSPAEFVRAVEQHRPEILAMSALLTTTMGEMARVIKALEKHHLRKSVRVLVG
EEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEC
GGPVTKEFAESIGADAYGSSAREAISIAQSLVRRCSDRERDYAC
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNGRTQIFQDIISAILRGEANGVLKSINAGLQMGMSADEILEEAMIPATIQTLDKYQGAD
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHCCCC
FYIPDVINASHAIEAGLFALKPYTKKSLQKRKKIVIGTVEGDIHDIGKNLVALSLGYAGF
EECCCHHCHHHHHHHHHHHCCCHHHHHHHHHHCEEEEECCCHHHHHHHHHHHHHHCCCCE
EVVDLGVNVSPAEFVRAVEQHRPEILAMSALLTTTMGEMARVIKALEKHHLRKSVRVLVG
EEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEC
GGPVTKEFAESIGADAYGSSAREAISIAQSLVRRCSDRERDYAC
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; Cobinamide; S-adenosyl-L-methionine [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 500-780 [C]

Specific activity: 3.8

Km value (mM): 0.0016 {S-adenosyl-L-methionine}} 0.00065 {S-adenosyl-L-methionine}} 0.06 {methyltetrahydrofolate}} 0.03 {methyltetrahydrofolate}} 0.016 {homocysteine}} 0.035 {S-methyltetrahydrofolate}} [C]

Substrates: 5-Methyltetrahydrofolate; L-Homocysteine [C]

Specific reaction: 5-Methyltetrahydrofolate + L-Homocysteine --> L-Methionine + 5,6,7,8-Tetrahydrofolate [C]

General reaction: Methyl group transfer [C]

Inhibitor: 5, 6-Dimethyl benzimidazole; alpha-Ribazole 3'-phosphate; Be2+; Cobinamide; Propyl iodide; Sr2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]