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The map label for this gene is yagI [H]

Identifier: 83590028

GI number: 83590028

Start: 1211436

End: 1212239

Strand: Reverse

Name: yagI [H]

Synonym: Moth_1180

Alternate gene names: 83590028

Gene position: 1212239-1211436 (Counterclockwise)

Preceding gene: 83590029

Following gene: 83590027

Centisome position: 46.11

GC content: 53.11

Gene sequence:

>804_bases
ATGGCCAAAGAAAAAATAAACGGCGAAGAAGGTAAAACAGTCCAGGCGGTAGAAAGGGCTTTAGCCATCATGGATGCCCT
GGCGGAAGCCGGTACCCCCATGGCCATCACCGACCTGGCCGATAAAGTCCAGTTAAATATAAGCACCGTACACCGGCTTC
TGAGCACCCTCATTGTCAGGGGGTATATCGAGCAAGAGCCGGAAACCAGCCGCTACAAGCTTGGGTTGAAGCTTTTTGCC
ATGGGGAAAACTGCCCTCTATGCCCAGGACCTGCAGACCATCGGCCGTCCCTTTCTGGAGGAACTGGTACGACGCTGTAA
TGAGACGGCCAACCTGGCCATCCTGGACGGGCGCGATGTAGTTTATATCGACCAGGTGGAATCCAACAATATCGTCATTG
TCCGCATGTTTGCCAGGGTGGGCAGCCGCGGTCCGGCCCATGCCACCGGTTCCGGTAAAATGCTTCTCAGTTCACTAACT
GATGACGAACTAGACAAGCTCTTTGACGGGGTTATTCTGGAACGCTACACCAGCGAGACAATTACGGACCTGGCTATCCT
CAAAAAAGAGTTGCAGCGTATCCGGCGCCAGGGCTATGCCATTGACCTGGGGGAAAGGGACGAGGATGTCCGCTGCGTTG
CCGCTCCAATTCGCAACCATGAGGGAAAGATAGTCGCCGCTATAAGTGTTTCCGGACCCCACACGCGCATTACCAATTAT
TATTTAAACAATGAGCTGGTTGATATTGTACTTGGTGTTGCCCGGGAGATGTCCAAACGCCTGGGCTATCAGGATAAAGG
GTAA

Upstream 100 bases:

>100_bases
TCAGTTTCACAAAAAGCTGGCCCAGGCCGATCCATGAGGACCCTGGGAGCGAAAGAAAACAACGCCAGCCGGCGTGAGTA
CCTTTGCGAGGGAAGCTGTA

Downstream 100 bases:

>100_bases
AATGAAAACTGCTTTCAAAATTAAATACAAGTTAATTTAAGCGTCCCTTATAAGTATACAAAATACGGGGCGCTTAATTA
TTTTTACATTATGAAAAACC

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MAKEKINGEEGKTVQAVERALAIMDALAEAGTPMAITDLADKVQLNISTVHRLLSTLIVRGYIEQEPETSRYKLGLKLFA
MGKTALYAQDLQTIGRPFLEELVRRCNETANLAILDGRDVVYIDQVESNNIVIVRMFARVGSRGPAHATGSGKMLLSSLT
DDELDKLFDGVILERYTSETITDLAILKKELQRIRRQGYAIDLGERDEDVRCVAAPIRNHEGKIVAAISVSGPHTRITNY
YLNNELVDIVLGVAREMSKRLGYQDKG

Sequences:

>Translated_267_residues
MAKEKINGEEGKTVQAVERALAIMDALAEAGTPMAITDLADKVQLNISTVHRLLSTLIVRGYIEQEPETSRYKLGLKLFA
MGKTALYAQDLQTIGRPFLEELVRRCNETANLAILDGRDVVYIDQVESNNIVIVRMFARVGSRGPAHATGSGKMLLSSLT
DDELDKLFDGVILERYTSETITDLAILKKELQRIRRQGYAIDLGERDEDVRCVAAPIRNHEGKIVAAISVSGPHTRITNY
YLNNELVDIVLGVAREMSKRLGYQDKG
>Mature_266_residues
AKEKINGEEGKTVQAVERALAIMDALAEAGTPMAITDLADKVQLNISTVHRLLSTLIVRGYIEQEPETSRYKLGLKLFAM
GKTALYAQDLQTIGRPFLEELVRRCNETANLAILDGRDVVYIDQVESNNIVIVRMFARVGSRGPAHATGSGKMLLSSLTD
DELDKLFDGVILERYTSETITDLAILKKELQRIRRQGYAIDLGERDEDVRCVAAPIRNHEGKIVAAISVSGPHTRITNYY
LNNELVDIVLGVAREMSKRLGYQDKG

Specific function: Unknown

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=250, Percent_Identity=38.8, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI1788131, Length=253, Percent_Identity=36.3636363636364, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1786715, Length=255, Percent_Identity=34.5098039215686, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI87082359, Length=251, Percent_Identity=34.6613545816733, Blast_Score=150, Evalue=6e-38,
Organism=Escherichia coli, GI1788581, Length=256, Percent_Identity=32.03125, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1790752, Length=255, Percent_Identity=26.6666666666667, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1789999, Length=265, Percent_Identity=28.3018867924528, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 29632; Mature: 29500

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKINGEEGKTVQAVERALAIMDALAEAGTPMAITDLADKVQLNISTVHRLLSTLIVR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHEECHHHHHHHHHHHHHH
GYIEQEPETSRYKLGLKLFAMGKTALYAQDLQTIGRPFLEELVRRCNETANLAILDGRDV
HHHCCCCCCCHHHCCEEEEEECCHHHHHHHHHHHCHHHHHHHHHHCCCCCCEEEECCCCE
VYIDQVESNNIVIVRMFARVGSRGPAHATGSGKMLLSSLTDDELDKLFDGVILERYTSET
EEEEEECCCCEEEEEEHHHHCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ITDLAILKKELQRIRRQGYAIDLGERDEDVRCVAAPIRNHEGKIVAAISVSGPHTRITNY
HHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCEEEEE
YLNNELVDIVLGVAREMSKRLGYQDKG
EECHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AKEKINGEEGKTVQAVERALAIMDALAEAGTPMAITDLADKVQLNISTVHRLLSTLIVR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHEECHHHHHHHHHHHHHH
GYIEQEPETSRYKLGLKLFAMGKTALYAQDLQTIGRPFLEELVRRCNETANLAILDGRDV
HHHCCCCCCCHHHCCEEEEEECCHHHHHHHHHHHCHHHHHHHHHHCCCCCCEEEECCCCE
VYIDQVESNNIVIVRMFARVGSRGPAHATGSGKMLLSSLTDDELDKLFDGVILERYTSET
EEEEEECCCCEEEEEEHHHHCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ITDLAILKKELQRIRRQGYAIDLGERDEDVRCVAAPIRNHEGKIVAAISVSGPHTRITNY
HHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCEEEEE
YLNNELVDIVLGVAREMSKRLGYQDKG
EECHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]