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The map label for this gene is gltX

Identifier: 83589980

GI number: 83589980

Start: 1160242

End: 1161714

Strand: Reverse

Name: gltX

Synonym: Moth_1132

Alternate gene names: 83589980

Gene position: 1161714-1160242 (Counterclockwise)

Preceding gene: 83589981

Following gene: 83589979

Centisome position: 44.19

GC content: 55.47

Gene sequence:

>1473_bases
TTGAATAAGGTTCGGGTTCGCTTTGCCCCCAGTCCCACTGGTAGCCTCCATATCGGTGGCGCCCGCACAGCTTTATTTAA
CTGGCTTTTTGCCCGGCACCACAACGGTACCTTTGTCCTGCGGATCGATGATACCGATACCGAGCGATCCACAGAAGCTT
CTTATAAGGAAATCCTGGCGGCCATGGGCTGGCTGGGCCTGGATTGGGATGAAGGGCCGGAAAAGGGAGGCCAGTTCGGG
CCTTACCTCCAGTCCCAGCGCCTGGAACTCTATCGCCGGGAGGCCGCCCGCTTGTTGAACGAGGGTAAAGCCTACTTGTG
TTACTGTACCGTTGAAGAGCTGGCCGAAAGGCGCCGGCAGGCCCAGGCCGAAGGCCGACCGCCCATGTATGACCGCCGCT
GCCGTTACCTCACTCCTGCCGACAGGACCCGCCTGGAACAGGAAGGAAGGCAACCTGTCATCCGCCTGGCCGTGCCGGAA
ACTGGGACAACCGTCGTTAAGGATCTTATCCGCGGCGATGTCGCCTTTGAAAATGCGACCATTGACGACTTTATTATTTT
TAAGTCCAACGGTATGCCCACCTATAATTTTGCTACGGTAATCGATGACCATTTAATGCAAATCAGCCATATCATCCGGG
CTGAAGAGCACCTCTCCAATACCCCTAAGCAGATTTTGGTTTACCAGGCCCTGGCATATGAACTGCCTGCCTTTGCCCAT
GTACCTATGATCCTGGCCCCTGATCGCAGCAAACTGAGTAAACGCCACGGGGCTACTTCGGTGGAGGAGTACAGGGATGA
GGGGTATTTACCCGAAGCCATCATTAATTACCTGGCCCTGCTGGGCTGGTCGCCGGAAGGCGAGGAAGAGATTATCCCCC
TGGAGAAAATAATCGAGCAATTTTCCCTGGAACGGGTATCCAAGAACGCCGCCATTTATGATACCAAGAAACTGACCTGG
ATCAATGGCCACTACCTGCGAGAAGGCAATCTGGACCGGATAACCAGGCTGGCTCTACCCTTTTTGCAGGCTAAAGGTTT
ACTACCAGACCCGTTACCCGAGAAGGATTACAACTATGTCCGCTCCGTTATCGCGGCCGTCCGCGACCGGGTCAAGACCC
TGGCAGAGGTTGCCGATGCCGCCAGTTACTTCTTTACTGATGTAACCAATTATGAAGAAAAGGGGATTCGTAAACACTTT
ACCAGGCCCGGGGCTGCGGCTTTGCTGGATGAAGCGCGGGAACGACTAGCTACCCTCCCTGAATTTAATGCCCAGGCAGC
CGAGGAGGCCTATCGTTCCCTAGCGGAAGGTAAGGGGATAAGCACAGGACAGTTATTCCATCCCACTCGTCTGGCAATCT
CCGGCCGTACCATGGGACCAGGACTCTTCGAGATTATGGAGCTCCTGGGCCGGGAAACTGTGCTCGCCCGCCTGGACCGG
GCCGCCAGGTGGATCCGGGAAAACCTGGCTTAA

Upstream 100 bases:

>100_bases
CGGCAGGGACGCCTTAATGCTAGTTCCCTTGCGGCGGCGACTATGGCCCGCCGCCTGGCAGACCAATACCAGGAGTTACT
TGGATAGGAGTGTTGATACC

Downstream 100 bases:

>100_bases
AAAACCAGATAATTAAAGGGCTGACAGCATTAGCTGCCAGCCCTTTAATTTTAATAGAAAAACCCTGCTAAGCAGGGTTT
TTCTACGCTTATGGCTGCGG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MNKVRVRFAPSPTGSLHIGGARTALFNWLFARHHNGTFVLRIDDTDTERSTEASYKEILAAMGWLGLDWDEGPEKGGQFG
PYLQSQRLELYRREAARLLNEGKAYLCYCTVEELAERRRQAQAEGRPPMYDRRCRYLTPADRTRLEQEGRQPVIRLAVPE
TGTTVVKDLIRGDVAFENATIDDFIIFKSNGMPTYNFATVIDDHLMQISHIIRAEEHLSNTPKQILVYQALAYELPAFAH
VPMILAPDRSKLSKRHGATSVEEYRDEGYLPEAIINYLALLGWSPEGEEEIIPLEKIIEQFSLERVSKNAAIYDTKKLTW
INGHYLREGNLDRITRLALPFLQAKGLLPDPLPEKDYNYVRSVIAAVRDRVKTLAEVADAASYFFTDVTNYEEKGIRKHF
TRPGAAALLDEARERLATLPEFNAQAAEEAYRSLAEGKGISTGQLFHPTRLAISGRTMGPGLFEIMELLGRETVLARLDR
AARWIRENLA

Sequences:

>Translated_490_residues
MNKVRVRFAPSPTGSLHIGGARTALFNWLFARHHNGTFVLRIDDTDTERSTEASYKEILAAMGWLGLDWDEGPEKGGQFG
PYLQSQRLELYRREAARLLNEGKAYLCYCTVEELAERRRQAQAEGRPPMYDRRCRYLTPADRTRLEQEGRQPVIRLAVPE
TGTTVVKDLIRGDVAFENATIDDFIIFKSNGMPTYNFATVIDDHLMQISHIIRAEEHLSNTPKQILVYQALAYELPAFAH
VPMILAPDRSKLSKRHGATSVEEYRDEGYLPEAIINYLALLGWSPEGEEEIIPLEKIIEQFSLERVSKNAAIYDTKKLTW
INGHYLREGNLDRITRLALPFLQAKGLLPDPLPEKDYNYVRSVIAAVRDRVKTLAEVADAASYFFTDVTNYEEKGIRKHF
TRPGAAALLDEARERLATLPEFNAQAAEEAYRSLAEGKGISTGQLFHPTRLAISGRTMGPGLFEIMELLGRETVLARLDR
AARWIRENLA
>Mature_490_residues
MNKVRVRFAPSPTGSLHIGGARTALFNWLFARHHNGTFVLRIDDTDTERSTEASYKEILAAMGWLGLDWDEGPEKGGQFG
PYLQSQRLELYRREAARLLNEGKAYLCYCTVEELAERRRQAQAEGRPPMYDRRCRYLTPADRTRLEQEGRQPVIRLAVPE
TGTTVVKDLIRGDVAFENATIDDFIIFKSNGMPTYNFATVIDDHLMQISHIIRAEEHLSNTPKQILVYQALAYELPAFAH
VPMILAPDRSKLSKRHGATSVEEYRDEGYLPEAIINYLALLGWSPEGEEEIIPLEKIIEQFSLERVSKNAAIYDTKKLTW
INGHYLREGNLDRITRLALPFLQAKGLLPDPLPEKDYNYVRSVIAAVRDRVKTLAEVADAASYFFTDVTNYEEKGIRKHF
TRPGAAALLDEARERLATLPEFNAQAAEEAYRSLAEGKGISTGQLFHPTRLAISGRTMGPGLFEIMELLGRETVLARLDR
AARWIRENLA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=503, Percent_Identity=33.3996023856859, Blast_Score=258, Evalue=9e-69,
Organism=Homo sapiens, GI62241042, Length=249, Percent_Identity=26.1044176706827, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI4826960, Length=297, Percent_Identity=26.2626262626263, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1788743, Length=489, Percent_Identity=40.2862985685072, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI226510925, Length=315, Percent_Identity=31.1111111111111, Blast_Score=135, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI71993394, Length=485, Percent_Identity=35.6701030927835, Blast_Score=268, Evalue=6e-72,
Organism=Caenorhabditis elegans, GI17511007, Length=285, Percent_Identity=25.6140350877193, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324540, Length=507, Percent_Identity=32.9388560157791, Blast_Score=264, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6324742, Length=309, Percent_Identity=29.126213592233, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6321192, Length=275, Percent_Identity=26.1818181818182, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24665321, Length=494, Percent_Identity=32.9959514170041, Blast_Score=281, Evalue=9e-76,
Organism=Drosophila melanogaster, GI17864488, Length=295, Percent_Identity=24.7457627118644, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_MOOTA (Q2RJE3)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_429989.1
- ProteinModelPortal:   Q2RJE3
- SMR:   Q2RJE3
- STRING:   Q2RJE3
- GeneID:   3833230
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_1132
- NMPDR:   fig|264732.9.peg.1114
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- PhylomeDB:   Q2RJE3
- ProtClustDB:   PRK01406
- BioCyc:   MTHE264732:MOTH_1132-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 55582; Mature: 55582

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 254-254

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVRVRFAPSPTGSLHIGGARTALFNWLFARHHNGTFVLRIDDTDTERSTEASYKEILA
CCCEEEEECCCCCCCEEECHHHHHHHHHHHHEECCCEEEEEECCCCCCCHHHHHHHHHHH
AMGWLGLDWDEGPEKGGQFGPYLQSQRLELYRREAARLLNEGKAYLCYCTVEELAERRRQ
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHH
AQAEGRPPMYDRRCRYLTPADRTRLEQEGRQPVIRLAVPETGTTVVKDLIRGDVAFENAT
HHCCCCCCCHHCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCHHCCCCC
IDDFIIFKSNGMPTYNFATVIDDHLMQISHIIRAEEHLSNTPKQILVYQALAYELPAFAH
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHC
VPMILAPDRSKLSKRHGATSVEEYRDEGYLPEAIINYLALLGWSPEGEEEIIPLEKIIEQ
CCEEECCCHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
FSLERVSKNAAIYDTKKLTWINGHYLREGNLDRITRLALPFLQAKGLLPDPLPEKDYNYV
HHHHHHHCCCEEEECCEEEEECCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
RSVIAAVRDRVKTLAEVADAASYFFTDVTNYEEKGIRKHFTRPGAAALLDEARERLATLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
EFNAQAAEEAYRSLAEGKGISTGQLFHPTRLAISGRTMGPGLFEIMELLGRETVLARLDR
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHCHHHHHHHHHH
AARWIRENLA
HHHHHHHHCC
>Mature Secondary Structure
MNKVRVRFAPSPTGSLHIGGARTALFNWLFARHHNGTFVLRIDDTDTERSTEASYKEILA
CCCEEEEECCCCCCCEEECHHHHHHHHHHHHEECCCEEEEEECCCCCCCHHHHHHHHHHH
AMGWLGLDWDEGPEKGGQFGPYLQSQRLELYRREAARLLNEGKAYLCYCTVEELAERRRQ
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHH
AQAEGRPPMYDRRCRYLTPADRTRLEQEGRQPVIRLAVPETGTTVVKDLIRGDVAFENAT
HHCCCCCCCHHCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCHHCCCCC
IDDFIIFKSNGMPTYNFATVIDDHLMQISHIIRAEEHLSNTPKQILVYQALAYELPAFAH
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHC
VPMILAPDRSKLSKRHGATSVEEYRDEGYLPEAIINYLALLGWSPEGEEEIIPLEKIIEQ
CCEEECCCHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
FSLERVSKNAAIYDTKKLTWINGHYLREGNLDRITRLALPFLQAKGLLPDPLPEKDYNYV
HHHHHHHCCCEEEECCEEEEECCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
RSVIAAVRDRVKTLAEVADAASYFFTDVTNYEEKGIRKHFTRPGAAALLDEARERLATLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
EFNAQAAEEAYRSLAEGKGISTGQLFHPTRLAISGRTMGPGLFEIMELLGRETVLARLDR
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHCHHHHHHHHHH
AARWIRENLA
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA