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The map label for this gene is murC [C]

Identifier: 83589845

GI number: 83589845

Start: 1023579

End: 1024946

Strand: Reverse

Name: murC [C]

Synonym: Moth_0996

Alternate gene names: 83589845

Gene position: 1024946-1023579 (Counterclockwise)

Preceding gene: 83589846

Following gene: 83589844

Centisome position: 38.99

GC content: 57.75

Gene sequence:

>1368_bases
ATGCTGCGGCGTATCCTGGCCATCCTGGCCGGCCGCCTGGTAGCTTTTTTATGCCGCTGCTTAAAAAAAGGCGGCACTTC
CCTTCCGGGTTTTCTGGCCCTGAAGTTGGACCCGGATTTGATAGAGGGCTTAATAGCAGGCTACCGCAAGGTAATCATAG
TCACAGGTACCAACGGTAAAACGACCACCACCAACCTCCTGGCCAGCATCCTGCGGGCATCAGGATTAAGAGTCGTTTCC
AATAGCGAAGGGGCCAATATGCCCGCCGGCGTTGCTACGGCCCTGCTAGGACAGCGGGGCGAAGTGGCTGTGCTGGAGGT
TGATGAAGGATCCCTGGCCCTTGTTACCGGCCAGGTGCAAGCTGATGTTGTGGTTGTAACTAATCTGTTGCGGGATCAAC
TGGATCGTTACCATGAATTGGAACAACTGGCCGAGGCCATAAAAATAGCCCTGGCCCATACCCCGGATGCCGCCCTGGTC
CTTAACGCCGATGATTCCCTGGTAACCTCCTTGGGAGATGGGCGTAGTACCGTGCGTTACTTCGGCCTGGCACGGACCCC
CTGGAGCCAGGAGACCACCAGGGAGGTGCTGGAGGGGCATATATGTCCCCATTGCCACCAGCCCTTGGGGTTTAAATTTT
ACCACTACAGCCACCTGGGGGATTATTTCTGCCCCCGCTGCTCCTACCGGCGTCCCCGAGCCGAGTATGAAGGCCGGGGG
CTCCAGTTAAAACCGGGAGGAACTGACTTTAACCTGGTGCATCCAGGTGGTGCCCTGTTCCTCCATACTCCCATGCCGGG
GATTTATAACGTCTACAATATCCTGGCCGCCGCAGGTACAGCGCTATATCTGGGGGTTGAGCCGGCAACCATTGTCCGGG
TGGTTGCTTCCTTCCTTCCCGGCCAGGGCCGGGCTGAAGCCTTCAACCTCCGGGACAGACGTATTACTTTAATGCTGGTC
AAAAACCCCACCGGCATGGGAGTGGCCCTCCGGACCCTGGCTACGGGCCGGCAAAAAATGGCTTTTCTCCTGGCCATTAA
TGACCTGGCCGCCGACGGCCGGGATGTCTCCTGGCTCTGGGACGCCGACCTTACTCCCCTGCTGGCAATCCCTGGCAATC
CCATCATTTGCGCCGGCCTCCGGGCCGGAGATATGGCCATCTGCCTTAAATACCAGGGGATAAAGGAGGCCGACCTGGAA
GTAATCCCCGACCCCGCAGCCAGTATAGAGTGTCTCCTGGCCAAACCGGTCAAGGAAGCCCTAATCCTCTGTACCTATAC
CAACCTGGCCGTTTACCGCCGTCTTTTGCAGCATAGGGGGGCTCGCAGTGAAGTTACACCTGGGACATCTCTACCCGGAG
TTTCTTAA

Upstream 100 bases:

>100_bases
TGGCTATTGCGCATGGAAGAACTGGTATCCTTCCTGAACATCCTGGGCCGCCGGCTGCAACGTTCCCGGCCGGCAACCGG
TTATAAGAAGGAGTGAGGAG

Downstream 100 bases:

>100_bases
CCTCTACGGAGACCGGGGCAATGTCTTGATCCTCTGCCGCCGCGCCCAGTGGCGGGGCATACAGGTAGAAGTAACCCCCA
TCTCCCTTGGTGATAAGCTG

Product: UDP-N-acetylmuramyl tripeptide synthase

Products: ADP; H+; Phosphate; UDP-N-acetylmuramoyl-L-alanine [C]

Alternate protein names: Mur Ligase Family Protein; UDP-N-Acetylmuramyl Tripeptide Synthetase; Mur Ligase; Ligase; UDP-N-Acetylmuramoylalanine-D-Glutamate Ligase; Mur Ligase Middle Domain Protein; Conserved Domain Protein; UDP-N-Acetylmuramyl Tripeptide Synthase-Like Protein; Cell Wall Biosynthesis Protein Mur Ligase Family; UDP-N-Acetylmuramyl Tripeptide Synthetase MurC; UDP-N-Acetylmuramyl Tripeptide Synthetase Related Protein; Peptide Ligase; UDP-N-Acetylmuramate-Alanine Ligase; UDP-N-Acetylmuramyl Tripeptide Synthetase Like; Mur Ligase Middle Domain-Containing Protein; Murein Ligase; UDP-N-Acetylmuramoyl-L-Alanyl-D-Glutamate-Lysine Ligase; Udp-N-Acetylmuramyl Tripeptide Synthetase; UDP-N-Acetylmuramyl Peptide Synthetase

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MLRRILAILAGRLVAFLCRCLKKGGTSLPGFLALKLDPDLIEGLIAGYRKVIIVTGTNGKTTTTNLLASILRASGLRVVS
NSEGANMPAGVATALLGQRGEVAVLEVDEGSLALVTGQVQADVVVVTNLLRDQLDRYHELEQLAEAIKIALAHTPDAALV
LNADDSLVTSLGDGRSTVRYFGLARTPWSQETTREVLEGHICPHCHQPLGFKFYHYSHLGDYFCPRCSYRRPRAEYEGRG
LQLKPGGTDFNLVHPGGALFLHTPMPGIYNVYNILAAAGTALYLGVEPATIVRVVASFLPGQGRAEAFNLRDRRITLMLV
KNPTGMGVALRTLATGRQKMAFLLAINDLAADGRDVSWLWDADLTPLLAIPGNPIICAGLRAGDMAICLKYQGIKEADLE
VIPDPAASIECLLAKPVKEALILCTYTNLAVYRRLLQHRGARSEVTPGTSLPGVS

Sequences:

>Translated_455_residues
MLRRILAILAGRLVAFLCRCLKKGGTSLPGFLALKLDPDLIEGLIAGYRKVIIVTGTNGKTTTTNLLASILRASGLRVVS
NSEGANMPAGVATALLGQRGEVAVLEVDEGSLALVTGQVQADVVVVTNLLRDQLDRYHELEQLAEAIKIALAHTPDAALV
LNADDSLVTSLGDGRSTVRYFGLARTPWSQETTREVLEGHICPHCHQPLGFKFYHYSHLGDYFCPRCSYRRPRAEYEGRG
LQLKPGGTDFNLVHPGGALFLHTPMPGIYNVYNILAAAGTALYLGVEPATIVRVVASFLPGQGRAEAFNLRDRRITLMLV
KNPTGMGVALRTLATGRQKMAFLLAINDLAADGRDVSWLWDADLTPLLAIPGNPIICAGLRAGDMAICLKYQGIKEADLE
VIPDPAASIECLLAKPVKEALILCTYTNLAVYRRLLQHRGARSEVTPGTSLPGVS
>Mature_455_residues
MLRRILAILAGRLVAFLCRCLKKGGTSLPGFLALKLDPDLIEGLIAGYRKVIIVTGTNGKTTTTNLLASILRASGLRVVS
NSEGANMPAGVATALLGQRGEVAVLEVDEGSLALVTGQVQADVVVVTNLLRDQLDRYHELEQLAEAIKIALAHTPDAALV
LNADDSLVTSLGDGRSTVRYFGLARTPWSQETTREVLEGHICPHCHQPLGFKFYHYSHLGDYFCPRCSYRRPRAEYEGRG
LQLKPGGTDFNLVHPGGALFLHTPMPGIYNVYNILAAAGTALYLGVEPATIVRVVASFLPGQGRAEAFNLRDRRITLMLV
KNPTGMGVALRTLATGRQKMAFLLAINDLAADGRDVSWLWDADLTPLLAIPGNPIICAGLRAGDMAICLKYQGIKEADLE
VIPDPAASIECLLAKPVKEALILCTYTNLAVYRRLLQHRGARSEVTPGTSLPGVS

Specific function: Cell Wall Formation. [C]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.8 [C]

Molecular weight: Translated: 49022; Mature: 49022

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRRILAILAGRLVAFLCRCLKKGGTSLPGFLALKLDPDLIEGLIAGYRKVIIVTGTNGK
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHCCCEEEEEEECCCCC
TTTTNLLASILRASGLRVVSNSEGANMPAGVATALLGQRGEVAVLEVDEGSLALVTGQVQ
CHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEECCCC
ADVVVVTNLLRDQLDRYHELEQLAEAIKIALAHTPDAALVLNADDSLVTSLGDGRSTVRY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCHHHHHCCCCCCCCEE
FGLARTPWSQETTREVLEGHICPHCHQPLGFKFYHYSHLGDYFCPRCSYRRPRAEYEGRG
EEECCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEHHCCCCCCCCCCCCCCCCCCCCCC
LQLKPGGTDFNLVHPGGALFLHTPMPGIYNVYNILAAAGTALYLGVEPATIVRVVASFLP
EEECCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCC
GQGRAEAFNLRDRRITLMLVKNPTGMGVALRTLATGRQKMAFLLAINDLAADGRDVSWLW
CCCCCEEECCCCCEEEEEEEECCCCCCHHHHHHHCCHHHEEEEEEEEHHCCCCCCEEEEE
DADLTPLLAIPGNPIICAGLRAGDMAICLKYQGIKEADLEVIPDPAASIECLLAKPVKEA
CCCCCEEEEECCCCEEEEECCCCCEEEEEEECCCCCCCCEECCCCCCCHHHHHHCCHHHE
LILCTYTNLAVYRRLLQHRGARSEVTPGTSLPGVS
EEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLRRILAILAGRLVAFLCRCLKKGGTSLPGFLALKLDPDLIEGLIAGYRKVIIVTGTNGK
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHCCCEEEEEEECCCCC
TTTTNLLASILRASGLRVVSNSEGANMPAGVATALLGQRGEVAVLEVDEGSLALVTGQVQ
CHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEECCCC
ADVVVVTNLLRDQLDRYHELEQLAEAIKIALAHTPDAALVLNADDSLVTSLGDGRSTVRY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCHHHHHCCCCCCCCEE
FGLARTPWSQETTREVLEGHICPHCHQPLGFKFYHYSHLGDYFCPRCSYRRPRAEYEGRG
EEECCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEHHCCCCCCCCCCCCCCCCCCCCCC
LQLKPGGTDFNLVHPGGALFLHTPMPGIYNVYNILAAAGTALYLGVEPATIVRVVASFLP
EEECCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCC
GQGRAEAFNLRDRRITLMLVKNPTGMGVALRTLATGRQKMAFLLAINDLAADGRDVSWLW
CCCCCEEECCCCCEEEEEEEECCCCCCHHHHHHHCCHHHEEEEEEEEHHCCCCCCEEEEE
DADLTPLLAIPGNPIICAGLRAGDMAICLKYQGIKEADLEVIPDPAASIECLLAKPVKEA
CCCCCEEEEECCCCEEEEECCCCCEEEEEEECCCCCCCCEECCCCCCCHHHHHHCCHHHE
LILCTYTNLAVYRRLLQHRGARSEVTPGTSLPGVS
EEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: (NH4)2SO4; Co2+; Mg2+; Mn2+ [C]

Kcat value (1/min): 1700 [C]

Specific activity: 2.94

Km value (mM): 0.22 {N-acetylmuramate}} 1 {dihydro-UDP-N-acetylmuramate}} 1.99 {L-Ser}} 1.2 {L-Ser}} 0.19 {beta-cyano-L-Ala}} 10 {Gly}} 7.89 {Gly}} 2.5 {Gly}} 70 {L-Val}} 0.173 {beta-chloro-L-Ala}} 62 {N-(2-aminopropionyl)piperidine}} 16

Substrates: L-Alanine; ATP; UDP-N-acetylmuramate [C]

Specific reaction: L-Alanine + ATP + UDP-N-acetylmuramate --> ADP + H+ + Phosphate + UDP-N-acetylmuramoyl-L-alanine [C]

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: 3, 3, 3-Trifluoro-DL-Ala; 3, 3-Difluoro-L-Ala; 3-Chloro-L-Ala; 3-Cyano-L-Ala; 3-Fluoro-L-Ala; Gly; KCl; L-2-Aminobutyrate; L-Allyl glycine; L-Cys; L-Lactate; L-Propargyl glycine; L-Ser; L-Vinyl glycine; N-Methyl -L-Ala; NaCl; NEM; p-Hydroxy mercuribenzoat

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA