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The map label for this gene is ykfA [H]

Identifier: 83589563

GI number: 83589563

Start: 743486

End: 744442

Strand: Reverse

Name: ykfA [H]

Synonym: Moth_0711

Alternate gene names: 83589563

Gene position: 744442-743486 (Counterclockwise)

Preceding gene: 83589564

Following gene: 83589546

Centisome position: 28.32

GC content: 61.44

Gene sequence:

>957_bases
ATGGAGAAATTAAAGCTGATCCTCAAACCGCCGGCCCTCAAAAAAGGCGCTACCATCGGCATCATAGCCCCGGCCAGTCC
CCTGGCGCAGCCGGAATACCTGGAACGGGGCCTGGAGTTCTGGCGCAGCCGGGGCTACCGGGTCCGTACCGGTAGGCATA
TAAGTAAAGCCGCCGGCTACCTGGCCGGGAATGATGCCGATCGCCTGGCCGACCTGCACTGGATGTTCCGGGACCTGGAA
ATAGGGGCCATAATTTGCCTCCGGGGCGGTTACGGCAGCCTCAGGCTCCTGGCCGGCCTCGACTATGATCTTGTCCTCTC
TCATCCGAAGATCCTGTTAGGCTACAGCGACATTACCGCCTTACATTTGGCCTTCAACAAAATCGCGGGGCTGGTTACTT
TTCACGGGCCCATGATCTACCCCGAGCTGGGCGGCCCGGTCCCGGACCCTTATACCGAAAAAGCCCTCTTTCGTACCCTG
GCAACAACCGCACCCCTGGGTACCATACCCCCGGCTCCGGAGTTGCCAGCTCCGGTGAGCATTATTCCCGGGCAGGCCGA
AGGGACGGTTACCGGCGGCAACCTCTCCCTGGTTACGGCAACTCTGGGTACGACCTTTGAAATCGATACCCGTGGCAGAA
TCCTTTTCCTGGAAGAAGTAGACGAAGCCCCCTATCGCCTGGACCGGATGCTCACCCAGTTGCAGCTGGCCGGCAAGCTG
GAGGAGGCGGCCGGGATTGTCTTCGGTTATTGTACCCGGTGCGACGATAACCGCCAGGGATTAACTGCCCTGGAAGTCAT
CACCGCCCGGCTCAAGCCCCTGGGCCGACCCTGTTTCTACGGCCTCCCGGCCGGCCATGTCAAACCCCAGGCCACCCTGC
CCCTTGGCATCCGTGCCCGCCTGGATGCCACGGCCTGTACCCTTACCTACCTGGAAGCAGCGACGGCGCCGATCTAA

Upstream 100 bases:

>100_bases
CTCGGCCGAGCAGCATAGCGGTCCAGGAAGGCCGGGGAGCGAAGGCGAGGCCATCGGTGATGGAGATAAGCATCGAAAAA
GACCCGGACGGAGGGAGTTC

Downstream 100 bases:

>100_bases
AACCGGCGCCGTCTTTCAGGGGAGGAAGTGTCCTATTAGCGAAAGGAAAACCATCACTCCAATCACAGCTATGGTCATTA
CAATGGCGGTACTCCTGGTC

Product: peptidase U61, LD-carboxypeptidase A

Products: NA

Alternate protein names: LD-carboxypeptidase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MEKLKLILKPPALKKGATIGIIAPASPLAQPEYLERGLEFWRSRGYRVRTGRHISKAAGYLAGNDADRLADLHWMFRDLE
IGAIICLRGGYGSLRLLAGLDYDLVLSHPKILLGYSDITALHLAFNKIAGLVTFHGPMIYPELGGPVPDPYTEKALFRTL
ATTAPLGTIPPAPELPAPVSIIPGQAEGTVTGGNLSLVTATLGTTFEIDTRGRILFLEEVDEAPYRLDRMLTQLQLAGKL
EEAAGIVFGYCTRCDDNRQGLTALEVITARLKPLGRPCFYGLPAGHVKPQATLPLGIRARLDATACTLTYLEAATAPI

Sequences:

>Translated_318_residues
MEKLKLILKPPALKKGATIGIIAPASPLAQPEYLERGLEFWRSRGYRVRTGRHISKAAGYLAGNDADRLADLHWMFRDLE
IGAIICLRGGYGSLRLLAGLDYDLVLSHPKILLGYSDITALHLAFNKIAGLVTFHGPMIYPELGGPVPDPYTEKALFRTL
ATTAPLGTIPPAPELPAPVSIIPGQAEGTVTGGNLSLVTATLGTTFEIDTRGRILFLEEVDEAPYRLDRMLTQLQLAGKL
EEAAGIVFGYCTRCDDNRQGLTALEVITARLKPLGRPCFYGLPAGHVKPQATLPLGIRARLDATACTLTYLEAATAPI
>Mature_318_residues
MEKLKLILKPPALKKGATIGIIAPASPLAQPEYLERGLEFWRSRGYRVRTGRHISKAAGYLAGNDADRLADLHWMFRDLE
IGAIICLRGGYGSLRLLAGLDYDLVLSHPKILLGYSDITALHLAFNKIAGLVTFHGPMIYPELGGPVPDPYTEKALFRTL
ATTAPLGTIPPAPELPAPVSIIPGQAEGTVTGGNLSLVTATLGTTFEIDTRGRILFLEEVDEAPYRLDRMLTQLQLAGKL
EEAAGIVFGYCTRCDDNRQGLTALEVITARLKPLGRPCFYGLPAGHVKPQATLPLGIRARLDATACTLTYLEAATAPI

Specific function: May be involved in the degradation of peptidoglycan by catalyzing the cleavage of the terminal D-alanine residue from cytoplasmic murein peptides [H]

COG id: COG1619

COG function: function code V; Uncharacterized proteins, homologs of microcin C7 resistance protein MccF

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S66 family [H]

Homologues:

Organism=Escherichia coli, GI1787441, Length=291, Percent_Identity=31.2714776632302, Blast_Score=122, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003507 [H]

Pfam domain/function: PF02016 Peptidase_S66 [H]

EC number: NA

Molecular weight: Translated: 34321; Mature: 34321

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLKLILKPPALKKGATIGIIAPASPLAQPEYLERGLEFWRSRGYRVRTGRHISKAAGY
CCCCEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEECCCCHHHHCCC
LAGNDADRLADLHWMFRDLEIGAIICLRGGYGSLRLLAGLDYDLVLSHPKILLGYSDITA
CCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCEEEEECCCEEEEECCHHHH
LHLAFNKIAGLVTFHGPMIYPELGGPVPDPYTEKALFRTLATTAPLGTIPPAPELPAPVS
HHHHHHHHHHEEEECCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEE
IIPGQAEGTVTGGNLSLVTATLGTTFEIDTRGRILFLEEVDEAPYRLDRMLTQLQLAGKL
EECCCCCCEEECCCEEEEEEECCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
EEAAGIVFGYCTRCDDNRQGLTALEVITARLKPLGRPCFYGLPAGHVKPQATLPLGIRAR
HHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCEEEE
LDATACTLTYLEAATAPI
CCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEKLKLILKPPALKKGATIGIIAPASPLAQPEYLERGLEFWRSRGYRVRTGRHISKAAGY
CCCCEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEECCCCHHHHCCC
LAGNDADRLADLHWMFRDLEIGAIICLRGGYGSLRLLAGLDYDLVLSHPKILLGYSDITA
CCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCEEEEECCCEEEEECCHHHH
LHLAFNKIAGLVTFHGPMIYPELGGPVPDPYTEKALFRTLATTAPLGTIPPAPELPAPVS
HHHHHHHHHHEEEECCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEE
IIPGQAEGTVTGGNLSLVTATLGTTFEIDTRGRILFLEEVDEAPYRLDRMLTQLQLAGKL
EECCCCCCEEECCCEEEEEEECCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
EEAAGIVFGYCTRCDDNRQGLTALEVITARLKPLGRPCFYGLPAGHVKPQATLPLGIRAR
HHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCEEEE
LDATACTLTYLEAATAPI
CCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]