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The map label for this gene is nirB [H]

Identifier: 83589370

GI number: 83589370

Start: 524367

End: 525077

Strand: Reverse

Name: nirB [H]

Synonym: Moth_0506

Alternate gene names: 83589370

Gene position: 525077-524367 (Counterclockwise)

Preceding gene: 83589372

Following gene: 83589369

Centisome position: 19.97

GC content: 61.46

Gene sequence:

>711_bases
ATGGGTGATGCTATTTTTAAACAGCGCTCCGGCTACCTGGGCGTGGGTATCGTGGCCAGATGCGGTATACTTACACCTGA
CCAGCTGGCCGGCCTTGGCGACCTGGCTCGTGCCCTTGACTGCCAGTACTGCAAATTGACGACCCGCCAGACCCTGATTT
TTATCATTCCGGAAGACAGGCTGGAAGACCTGCGGGCCGGCGTTACCGCCCTGGGCCTACAGGTTGGTGTCTTTGGCGAA
ATAGTGCGCAACATCAAAGCCTGCGCCGGCAACAAAGACCTCTGCCAGCGCTCTTTAAGCGATGTCTTTGAACTGGGGGG
CGTGCTCCAGGACCGCTTTATGAACCGTCCCACCCCCTGCGATTTTAAAATCGCCTTGGCCGGATGCCACCGGGGCTGCA
CCGATCCCCAGTGTGCCGACTACGGCATTATTGCCACCGGCAACGATACCTACGACGTCTATCTAGGGGGACGGGGCGGC
AGCCGCAAACCCATCCACGCCACCCGAATTGCCACCGGCATAACCGGCAAGGGGGTCGAGGACCTCCTGGCCTGGATCCT
TGAACGCTATGACGCCCTGGCCGAACCCCGGGAGCGCCTCTGCAACACCATCGCCCGGGTGGGGCTGGAGGCGTTCTTGC
CGCCGGAGGGCTTCCTTGAAGGCTACCGGCCCCGGGAAGACAACGCCTTCCTCACCTTCGCTGGGCTGTGA

Upstream 100 bases:

>100_bases
TTCAGGCTTCCACTTTAGTATGGCCCGCCAAAGTTTACCCTATAAGCAGGATTTCCCGCCCTTATGAAGAATTATTACTG
ATAACAATGGAGGTGGAAGT

Downstream 100 bases:

>100_bases
GGTAGGCGGAATTTCAGATTGGAGGTTTTTCAAGATGTCCGTCCGTAATATTGATCTCGATAAGATGCAACACTTTATCG
ATCGCTGCTGTAAGACGGAG

Product: nitrite and sulphite reductase 4Fe-4S region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MGDAIFKQRSGYLGVGIVARCGILTPDQLAGLGDLARALDCQYCKLTTRQTLIFIIPEDRLEDLRAGVTALGLQVGVFGE
IVRNIKACAGNKDLCQRSLSDVFELGGVLQDRFMNRPTPCDFKIALAGCHRGCTDPQCADYGIIATGNDTYDVYLGGRGG
SRKPIHATRIATGITGKGVEDLLAWILERYDALAEPRERLCNTIARVGLEAFLPPEGFLEGYRPREDNAFLTFAGL

Sequences:

>Translated_236_residues
MGDAIFKQRSGYLGVGIVARCGILTPDQLAGLGDLARALDCQYCKLTTRQTLIFIIPEDRLEDLRAGVTALGLQVGVFGE
IVRNIKACAGNKDLCQRSLSDVFELGGVLQDRFMNRPTPCDFKIALAGCHRGCTDPQCADYGIIATGNDTYDVYLGGRGG
SRKPIHATRIATGITGKGVEDLLAWILERYDALAEPRERLCNTIARVGLEAFLPPEGFLEGYRPREDNAFLTFAGL
>Mature_235_residues
GDAIFKQRSGYLGVGIVARCGILTPDQLAGLGDLARALDCQYCKLTTRQTLIFIIPEDRLEDLRAGVTALGLQVGVFGEI
VRNIKACAGNKDLCQRSLSDVFELGGVLQDRFMNRPTPCDFKIALAGCHRGCTDPQCADYGIIATGNDTYDVYLGGRGGS
RKPIHATRIATGITGKGVEDLLAWILERYDALAEPRERLCNTIARVGLEAFLPPEGFLEGYRPREDNAFLTFAGL

Specific function: Nitrate assimilation (denitrification); second step. [C]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789765, Length=169, Percent_Identity=31.9526627218935, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 25646; Mature: 25515

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDAIFKQRSGYLGVGIVARCGILTPDQLAGLGDLARALDCQYCKLTTRQTLIFIIPEDR
CCCCHHHHCCCCCCCHHHHHCCCCCHHHHHCHHHHHHHHCCHHHEECCCCEEEEEECHHH
LEDLRAGVTALGLQVGVFGEIVRNIKACAGNKDLCQRSLSDVFELGGVLQDRFMNRPTPC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DFKIALAGCHRGCTDPQCADYGIIATGNDTYDVYLGGRGGSRKPIHATRIATGITGKGVE
CEEEEEECCCCCCCCCCCCCCCEEEECCCEEEEEEECCCCCCCCEEHHHHHCCCCCCCHH
DLLAWILERYDALAEPRERLCNTIARVGLEAFLPPEGFLEGYRPREDNAFLTFAGL
HHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHCCCCHHHHCCCCCCCCCEEEEEECC
>Mature Secondary Structure 
GDAIFKQRSGYLGVGIVARCGILTPDQLAGLGDLARALDCQYCKLTTRQTLIFIIPEDR
CCCHHHHCCCCCCCHHHHHCCCCCHHHHHCHHHHHHHHCCHHHEECCCCEEEEEECHHH
LEDLRAGVTALGLQVGVFGEIVRNIKACAGNKDLCQRSLSDVFELGGVLQDRFMNRPTPC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DFKIALAGCHRGCTDPQCADYGIIATGNDTYDVYLGGRGGSRKPIHATRIATGITGKGVE
CEEEEEECCCCCCCCCCCCCCCEEEECCCEEEEEEECCCCCCCCEEHHHHHCCCCCCCHH
DLLAWILERYDALAEPRERLCNTIARVGLEAFLPPEGFLEGYRPREDNAFLTFAGL
HHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHCCCCHHHHCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2543955; 2200672; 9278503; 2445993 [H]