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The map label for this gene is thiI

Identifier: 83589067

GI number: 83589067

Start: 193951

End: 195123

Strand: Reverse

Name: thiI

Synonym: Moth_0198

Alternate gene names: 83589067

Gene position: 195123-193951 (Counterclockwise)

Preceding gene: 83589068

Following gene: 83589066

Centisome position: 7.42

GC content: 62.4

Gene sequence:

>1173_bases
ATGTATACATCCCTACTGGTCCGTTACGGCGAGATCAGCCTCAAGGGCAACAACCGCCCATACTTTGAAGATAAACTCCT
GGCCAACATGCGCCGGGCCCTGGCCGGCCTGCCGCCCCGCAGAATGCGCAAGACCTTCGGCCGCGTCTTCGTGGAGCTCC
ATGACGACCTGGAAGCCGTAGCCCGGCGTTTGCAGCGCGTCTTTGGCATCGTCTCCATGAGCCCGGTAGCCACAGCTCCC
CTGGAGCTGGAAGCCATCAAAAAAGCCGCCCTGGCCGTCTTAAAGGATTCCCCCGGCAGCACCTTTAAAGTCCAGGCCCA
GCGGCCCAATAAACGCTTTCCCCTCACCTCACCTGAAGTCAACCAGGAACTGGGGGCCTACCTCCTCACCCACAGCCAGG
GCCAACGGGTAGACGTTCACCATCCCGACCGGGTTATCCATGTGGAAATCCGCGACGAAGGGGCCTATATCTACTCCCGC
ATCATCCCCGGACCCGGCGGCCTGCCCGTAGGGGTCACCGGCCGGGGGCTGCTCCTGATCTCCGGCGGCATCGACAGCCC
GGTGGCCGGCTATATGGGCATGAAACGGGGCCTGGAACTCACGGCCCTCCATTTTCACAGCTTCCCTTTTACCAGCGAGC
GCTCCAAGGAAAAGGTCATCGACCTCTGCCGGGTCCTGGCAGGCTACAGCGGACCTTTGCGCCTGGTGGTGGCCCCCTTT
ACCAATATCCAGAAGGCCATCCGCCAGAACTGCCCCCAGGAGTTTTACGTCACCATCATGCGGCGGATGATGTTCCGCAT
CGCCAGGGCGGTGGCTGCCAAAGAGGAGGCCCCGGCCATCCTCACGGGGGAGAGCCTGGGCCAGGTGGCCAGCCAGACCC
TCCAGAGCATGGCGGTAATCAACAAGGTGGTCGACCTGCCGGTCTTAAGGCCCCTGGTGGCCTGGGACAAGAGCGAGATT
ATCGAGGTGGCCCGCCGCATCGGCACCTACGACATCTCCATCCGGCCCTACGAGGACTGCTGCACCCTCTTTGTCCCCAA
ACACCCGGCCACCAAACCGCCCCTGGCCCGGGTGGAAGCGGCCGAAAAGAATCTGGCCGTGGTGGAACTCGTCGCGGAGT
GCCTGGAAAATCTAGAAATCCTGACGGTGGAGCCCGAAGCTGACGTAGTGTAA

Upstream 100 bases:

>100_bases
TGGCGGCTACAGGCGGAGCCTGAAGCCTGTATCGCCAGCATCGTAGCAGAGTAACGCCATTAGCAGCCACTTCTGGAAAA
CCTATTTTTGGAGGTTTGAC

Downstream 100 bases:

>100_bases
GCTGTGGGATCCTCCGAACCAAGTCGCCCTTCGCCTGTACCCGCCAGTCACTTGCCCAACTTGCATTTTCGTGGTTCGCT
GGCGGTGGCGTCAGCCCCCA

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MYTSLLVRYGEISLKGNNRPYFEDKLLANMRRALAGLPPRRMRKTFGRVFVELHDDLEAVARRLQRVFGIVSMSPVATAP
LELEAIKKAALAVLKDSPGSTFKVQAQRPNKRFPLTSPEVNQELGAYLLTHSQGQRVDVHHPDRVIHVEIRDEGAYIYSR
IIPGPGGLPVGVTGRGLLLISGGIDSPVAGYMGMKRGLELTALHFHSFPFTSERSKEKVIDLCRVLAGYSGPLRLVVAPF
TNIQKAIRQNCPQEFYVTIMRRMMFRIARAVAAKEEAPAILTGESLGQVASQTLQSMAVINKVVDLPVLRPLVAWDKSEI
IEVARRIGTYDISIRPYEDCCTLFVPKHPATKPPLARVEAAEKNLAVVELVAECLENLEILTVEPEADVV

Sequences:

>Translated_390_residues
MYTSLLVRYGEISLKGNNRPYFEDKLLANMRRALAGLPPRRMRKTFGRVFVELHDDLEAVARRLQRVFGIVSMSPVATAP
LELEAIKKAALAVLKDSPGSTFKVQAQRPNKRFPLTSPEVNQELGAYLLTHSQGQRVDVHHPDRVIHVEIRDEGAYIYSR
IIPGPGGLPVGVTGRGLLLISGGIDSPVAGYMGMKRGLELTALHFHSFPFTSERSKEKVIDLCRVLAGYSGPLRLVVAPF
TNIQKAIRQNCPQEFYVTIMRRMMFRIARAVAAKEEAPAILTGESLGQVASQTLQSMAVINKVVDLPVLRPLVAWDKSEI
IEVARRIGTYDISIRPYEDCCTLFVPKHPATKPPLARVEAAEKNLAVVELVAECLENLEILTVEPEADVV
>Mature_390_residues
MYTSLLVRYGEISLKGNNRPYFEDKLLANMRRALAGLPPRRMRKTFGRVFVELHDDLEAVARRLQRVFGIVSMSPVATAP
LELEAIKKAALAVLKDSPGSTFKVQAQRPNKRFPLTSPEVNQELGAYLLTHSQGQRVDVHHPDRVIHVEIRDEGAYIYSR
IIPGPGGLPVGVTGRGLLLISGGIDSPVAGYMGMKRGLELTALHFHSFPFTSERSKEKVIDLCRVLAGYSGPLRLVVAPF
TNIQKAIRQNCPQEFYVTIMRRMMFRIARAVAAKEEAPAILTGESLGQVASQTLQSMAVINKVVDLPVLRPLVAWDKSEI
IEVARRIGTYDISIRPYEDCCTLFVPKHPATKPPLARVEAAEKNLAVVELVAECLENLEILTVEPEADVV

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=378, Percent_Identity=31.2169312169312, Blast_Score=153, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_MOOTA (Q2RM06)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_429076.1
- HSSP:   Q81KU0
- ProteinModelPortal:   Q2RM06
- SMR:   Q2RM06
- STRING:   Q2RM06
- GeneID:   3832271
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_0198
- NMPDR:   fig|264732.9.peg.194
- eggNOG:   COG0301
- HOGENOM:   HBG646072
- OMA:   ESIGQVA
- ProtClustDB:   PRK01565
- BioCyc:   MTHE264732:MOTH_0198-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP

EC number: =2.8.1.4

Molecular weight: Translated: 43238; Mature: 43238

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS51165 THUMP

Important sites: BINDING 261-261 BINDING 283-283 BINDING 292-292

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTSLLVRYGEISLKGNNRPYFEDKLLANMRRALAGLPPRRMRKTFGRVFVELHDDLEAV
CCHHHEEEECEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
ARRLQRVFGIVSMSPVATAPLELEAIKKAALAVLKDSPGSTFKVQAQRPNKRFPLTSPEV
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCH
NQELGAYLLTHSQGQRVDVHHPDRVIHVEIRDEGAYIYSRIIPGPGGLPVGVTGRGLLLI
HHHHHHEEEECCCCCEEECCCCCEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEEEE
SGGIDSPVAGYMGMKRGLELTALHFHSFPFTSERSKEKVIDLCRVLAGYSGPLRLVVAPF
ECCCCCCHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECH
TNIQKAIRQNCPQEFYVTIMRRMMFRIARAVAAKEEAPAILTGESLGQVASQTLQSMAVI
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHH
NKVVDLPVLRPLVAWDKSEIIEVARRIGTYDISIRPYEDCCTLFVPKHPATKPPLARVEA
HHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEECCHHHHHEEEECCCCCCCCCHHHHHH
AEKNLAVVELVAECLENLEILTVEPEADVV
HHCCHHHHHHHHHHHCCCEEEEECCCCCCC
>Mature Secondary Structure
MYTSLLVRYGEISLKGNNRPYFEDKLLANMRRALAGLPPRRMRKTFGRVFVELHDDLEAV
CCHHHEEEECEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
ARRLQRVFGIVSMSPVATAPLELEAIKKAALAVLKDSPGSTFKVQAQRPNKRFPLTSPEV
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCH
NQELGAYLLTHSQGQRVDVHHPDRVIHVEIRDEGAYIYSRIIPGPGGLPVGVTGRGLLLI
HHHHHHEEEECCCCCEEECCCCCEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEEEE
SGGIDSPVAGYMGMKRGLELTALHFHSFPFTSERSKEKVIDLCRVLAGYSGPLRLVVAPF
ECCCCCCHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECH
TNIQKAIRQNCPQEFYVTIMRRMMFRIARAVAAKEEAPAILTGESLGQVASQTLQSMAVI
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHH
NKVVDLPVLRPLVAWDKSEIIEVARRIGTYDISIRPYEDCCTLFVPKHPATKPPLARVEA
HHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEECCHHHHHEEEECCCCCCCCCHHHHHH
AEKNLAVVELVAECLENLEILTVEPEADVV
HHCCHHHHHHHHHHHCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA