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The map label for this gene is aroA

Identifier: 83313362

GI number: 83313362

Start: 4673374

End: 4674714

Strand: Reverse

Name: aroA

Synonym: amb4263

Alternate gene names: 83313362

Gene position: 4674714-4673374 (Counterclockwise)

Preceding gene: 83313364

Following gene: 83313361

Centisome position: 94.11

GC content: 69.95

Gene sequence:

>1341_bases
GTGATCATGGCCAAGCCGCTTTCATCCCGTCGGGCCGCACCTTTGGCCGGTTCGGCGCCCGTTCCGGGCGACAAGTCCAT
CTCGCACCGCGCCCTGATGCTGGGTGCCCTGGCGGTGGGTGAAAGCGTGGTCACCGGTCTGCTGGAAGGCGACGATGTTT
TACGCACCGCCGCCTGCATGCGGGCGCTGGGCGCCGAGGTCGAGCGCCGCGACGACGGATCGTGGCGGCTGTTCGGGCGC
GGCGTCGGCGGGCTGATGGAGCCGGCCGATGTTCTCGACATGGGCAATTCCGGCACCGGGGCGCGGCTGCTGATGGGCCT
GGTGGCCACCCATCCCTTCACCTGTTTTTTCACCGGCGACGGCTCGTTGCGCTCGCGGCCCATGCGCCGGGTGATCGATC
CGCTGTCGCGCATGGGGGCGCGCTTCGTCAGCCGTGACGGCGGCCGCCTGCCCCTGGCGGTGACCGGCACGGCGCAGCCC
ACGCCCATCACCTACGAGCTTCCCGTGGCCTCGGCCCAGGTGAAGTCGGCCATCATGCTGGCCGGTCTCAACACCGCCGG
CGAGACCACGGTGATCGAGCGGGAGGCCACCCGTGACCACACCGAACTGATGCTCCGGAATTTCGGCGCCACCGTGCGGG
TCGAGGACGCCGAGGGCGGTGGCCGCGCCATTACCGTGGTGGGCTTTCCGGAACTGACCGGCCGGCCGGTGGTGGTGCCC
GCCGATCCCTCCTCGGCGGCCTTCCCGGTGGTGGCCGCCCTGCTGGTGGAGGGCTCCGAGATCCGTCTGCCAAACGTCGG
CACCAATCCCTTGCGCACCGGGCTGTACCAGACCCTGCTGGAAATGGGCGCCGACATTCGCTTCGACCATCCCCGCGATC
AGGCGGGCGAGCCGGTGGCCGATCTGGTGGTGCGCTCGTCGCGCCTCAAGGGCGTGGACGTGCCGGCCGAGCGAGCGCCG
TCCATGATCGACGAATACCCCATCCTGGCCGTGGCCGCCGCCTTCGCCGAGGGCACCACCCGCATGCGGGGATTGGGCGA
GCTGCGGGTCAAGGAAAGCGACCGTCTGGCCGCCATGGCGCGCGGCCTGGCCGCCTGCGGCGTGGCGGTGGAGGAGGAGA
AGGATGCCCTCATCGTCCACGGGACCGGACGCATCCCCGACGGCGACGCCACCGTCACCACCCATTTCGATCACCGCATC
GCCATGTCGTTCCTGGTGATGGGCATGGCCTCGGCCCGGCCCGTGGCCGTGGACGATTCCGAAGCCATCGACACCAGCTT
CCCCGCCTTCGTCGAACTGATGAACGGGCTGGGGGCGAAGATTTCCGGAGACAATCCGTGA

Upstream 100 bases:

>100_bases
AGCGTGGGCGGTGCAGCATGGAACGCGGGTCTATGACGCGGAAGGCGCCTGTGATAGAGCTTCGGCGGGCAGCTTTTCCC
CTTGCCGCTGGTTATCAGGA

Downstream 100 bases:

>100_bases
GCACTATCATCGCCATCGACGGCCCCGCGGCGGCCGGCAAGGGGACTCTGGCGCGGCGTCTGGCCGCTGAATTGGGCTTC
GACTATCTCGACACCGGCCT

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MIMAKPLSSRRAAPLAGSAPVPGDKSISHRALMLGALAVGESVVTGLLEGDDVLRTAACMRALGAEVERRDDGSWRLFGR
GVGGLMEPADVLDMGNSGTGARLLMGLVATHPFTCFFTGDGSLRSRPMRRVIDPLSRMGARFVSRDGGRLPLAVTGTAQP
TPITYELPVASAQVKSAIMLAGLNTAGETTVIEREATRDHTELMLRNFGATVRVEDAEGGGRAITVVGFPELTGRPVVVP
ADPSSAAFPVVAALLVEGSEIRLPNVGTNPLRTGLYQTLLEMGADIRFDHPRDQAGEPVADLVVRSSRLKGVDVPAERAP
SMIDEYPILAVAAAFAEGTTRMRGLGELRVKESDRLAAMARGLAACGVAVEEEKDALIVHGTGRIPDGDATVTTHFDHRI
AMSFLVMGMASARPVAVDDSEAIDTSFPAFVELMNGLGAKISGDNP

Sequences:

>Translated_446_residues
MIMAKPLSSRRAAPLAGSAPVPGDKSISHRALMLGALAVGESVVTGLLEGDDVLRTAACMRALGAEVERRDDGSWRLFGR
GVGGLMEPADVLDMGNSGTGARLLMGLVATHPFTCFFTGDGSLRSRPMRRVIDPLSRMGARFVSRDGGRLPLAVTGTAQP
TPITYELPVASAQVKSAIMLAGLNTAGETTVIEREATRDHTELMLRNFGATVRVEDAEGGGRAITVVGFPELTGRPVVVP
ADPSSAAFPVVAALLVEGSEIRLPNVGTNPLRTGLYQTLLEMGADIRFDHPRDQAGEPVADLVVRSSRLKGVDVPAERAP
SMIDEYPILAVAAAFAEGTTRMRGLGELRVKESDRLAAMARGLAACGVAVEEEKDALIVHGTGRIPDGDATVTTHFDHRI
AMSFLVMGMASARPVAVDDSEAIDTSFPAFVELMNGLGAKISGDNP
>Mature_446_residues
MIMAKPLSSRRAAPLAGSAPVPGDKSISHRALMLGALAVGESVVTGLLEGDDVLRTAACMRALGAEVERRDDGSWRLFGR
GVGGLMEPADVLDMGNSGTGARLLMGLVATHPFTCFFTGDGSLRSRPMRRVIDPLSRMGARFVSRDGGRLPLAVTGTAQP
TPITYELPVASAQVKSAIMLAGLNTAGETTVIEREATRDHTELMLRNFGATVRVEDAEGGGRAITVVGFPELTGRPVVVP
ADPSSAAFPVVAALLVEGSEIRLPNVGTNPLRTGLYQTLLEMGADIRFDHPRDQAGEPVADLVVRSSRLKGVDVPAERAP
SMIDEYPILAVAAAFAEGTTRMRGLGELRVKESDRLAAMARGLAACGVAVEEEKDALIVHGTGRIPDGDATVTTHFDHRI
AMSFLVMGMASARPVAVDDSEAIDTSFPAFVELMNGLGAKISGDNP

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=446, Percent_Identity=28.6995515695067, Blast_Score=121, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320332, Length=478, Percent_Identity=29.9163179916318, Blast_Score=129, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_MAGSA (Q2VZA8)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_423626.1
- HSSP:   P22487
- STRING:   Q2VZA8
- GeneID:   3802778
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb4263
- NMPDR:   fig|342108.5.peg.3832
- eggNOG:   COG0128
- HOGENOM:   HBG646626
- OMA:   LSRNHTE
- ProtClustDB:   PRK02427
- BioCyc:   MMAG342108:AMB4263-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 46870; Mature: 46870

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMAKPLSSRRAAPLAGSAPVPGDKSISHRALMLGALAVGESVVTGLLEGDDVLRTAACM
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
RALGAEVERRDDGSWRLFGRGVGGLMEPADVLDMGNSGTGARLLMGLVATHPFTCFFTGD
HHHCCHHHCCCCCCEEEEECCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCEEEEEECC
GSLRSRPMRRVIDPLSRMGARFVSRDGGRLPLAVTGTAQPTPITYELPVASAQVKSAIML
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECCCCHHHHHHEEEE
AGLNTAGETTVIEREATRDHTELMLRNFGATVRVEDAEGGGRAITVVGFPELTGRPVVVP
EECCCCCCCEEEECHHCCHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCCCEEEEE
ADPSSAAFPVVAALLVEGSEIRLPNVGTNPLRTGLYQTLLEMGADIRFDHPRDQAGEPVA
CCCCCCHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHCCCCEECCCCCCCCCCCHH
DLVVRSSRLKGVDVPAERAPSMIDEYPILAVAAAFAEGTTRMRGLGELRVKESDRLAAMA
HHHHHHHCCCCCCCCHHHCCHHHHCCHHHHHHHHHHHCHHHHCCCCCEEECCCHHHHHHH
RGLAACGVAVEEEKDALIVHGTGRIPDGDATVTTHFDHRIAMSFLVMGMASARPVAVDDS
HHHHHHCCEEECCCCEEEEEECCCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCEEECCC
EAIDTSFPAFVELMNGLGAKISGDNP
CHHCCCHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MIMAKPLSSRRAAPLAGSAPVPGDKSISHRALMLGALAVGESVVTGLLEGDDVLRTAACM
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
RALGAEVERRDDGSWRLFGRGVGGLMEPADVLDMGNSGTGARLLMGLVATHPFTCFFTGD
HHHCCHHHCCCCCCEEEEECCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCEEEEEECC
GSLRSRPMRRVIDPLSRMGARFVSRDGGRLPLAVTGTAQPTPITYELPVASAQVKSAIML
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECCCCHHHHHHEEEE
AGLNTAGETTVIEREATRDHTELMLRNFGATVRVEDAEGGGRAITVVGFPELTGRPVVVP
EECCCCCCCEEEECHHCCHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCCCEEEEE
ADPSSAAFPVVAALLVEGSEIRLPNVGTNPLRTGLYQTLLEMGADIRFDHPRDQAGEPVA
CCCCCCHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHCCCCEECCCCCCCCCCCHH
DLVVRSSRLKGVDVPAERAPSMIDEYPILAVAAAFAEGTTRMRGLGELRVKESDRLAAMA
HHHHHHHCCCCCCCCHHHCCHHHHCCHHHHHHHHHHHCHHHHCCCCCEEECCCHHHHHHH
RGLAACGVAVEEEKDALIVHGTGRIPDGDATVTTHFDHRIAMSFLVMGMASARPVAVDDS
HHHHHHCCEEECCCCEEEEEECCCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCEEECCC
EAIDTSFPAFVELMNGLGAKISGDNP
CHHCCCHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA