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The map label for this gene is folE

Identifier: 83313322

GI number: 83313322

Start: 4633732

End: 4634427

Strand: Reverse

Name: folE

Synonym: amb4223

Alternate gene names: 83313322

Gene position: 4634427-4633732 (Counterclockwise)

Preceding gene: 83313323

Following gene: 83313319

Centisome position: 93.3

GC content: 66.24

Gene sequence:

>696_bases
ATGCCCGATCGTGACCACCGCGAAAGGAAAGCCGCCATGAGCAAGGACGCCACCTCTCTCACCCTGCCCAGGGAGCTTCT
CCCCGCTACCGGCTCCCGCCTGGACAAGCCCTCGCGGGAAGAGGCCGAGGAGGCGGTGCGCACCCTGCTGCGCTGGGCCG
GCGACGATCCCGACCGCGAGGGGCTGGTGGGCACTCCCGACCGGGTGGTGCGCGCCTACGAGGAGTTTTTCTCGGGCTAC
GACCAGGACCCCACCGAGATTCTGCACCGCACCTTCGAGGAAACCGACGGCTACGACGAGATGGTGCTGCTGCGCGATAT
CCGCCTGGAATCCCATTGCGAGCATCACGTGGTGCCGATCATCGGCAAGGCCCATGTGGCCTATCTGCCCAACCGCCGGG
TGGTGGGCATCTCCAAGCTGGCCCGCGTGGTGGAGATCTATTCGCGCCGCCTGCAGATCCAGGAAAAGCTGACGGCCCAG
ATCGCCAATACCATCAACGACGTGCTCCAGCCCAAGGGCGTGGCCGTGGTCATCGAAGCCGCCCACCAGTGCATGACCAC
CCGCGGCATCCACAAGCCCGGCGTCACCATGGTCACCAGCCGCATGCTCGGCGCCTTCCGCGAGGACCCCGCTACCCGCC
GGGAATTCATGGCGCTAATTCACAACCAGCACTCGGGCGGGCTGTCGAACGTGTAG

Upstream 100 bases:

>100_bases
CGGCCTTCGACATCGCCATTCCCGCTTTCTCCCTGGACAGCCCGCACGAGAAGCCCCGGCTGAACTAGCCGGGCTTGTAA
AATCCGAAAGGCGCAGCCAC

Downstream 100 bases:

>100_bases
CCGGGTTAGGCAGGGCGGCCGCCCCGGTCCTTCGCGCCCCCCAGGGTTGGCGCGCCGCGTGCATTGATACCCCCCGACAT
GACTGCAACCGGGGGCGTCA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MPDRDHRERKAAMSKDATSLTLPRELLPATGSRLDKPSREEAEEAVRTLLRWAGDDPDREGLVGTPDRVVRAYEEFFSGY
DQDPTEILHRTFEETDGYDEMVLLRDIRLESHCEHHVVPIIGKAHVAYLPNRRVVGISKLARVVEIYSRRLQIQEKLTAQ
IANTINDVLQPKGVAVVIEAAHQCMTTRGIHKPGVTMVTSRMLGAFREDPATRREFMALIHNQHSGGLSNV

Sequences:

>Translated_231_residues
MPDRDHRERKAAMSKDATSLTLPRELLPATGSRLDKPSREEAEEAVRTLLRWAGDDPDREGLVGTPDRVVRAYEEFFSGY
DQDPTEILHRTFEETDGYDEMVLLRDIRLESHCEHHVVPIIGKAHVAYLPNRRVVGISKLARVVEIYSRRLQIQEKLTAQ
IANTINDVLQPKGVAVVIEAAHQCMTTRGIHKPGVTMVTSRMLGAFREDPATRREFMALIHNQHSGGLSNV
>Mature_230_residues
PDRDHRERKAAMSKDATSLTLPRELLPATGSRLDKPSREEAEEAVRTLLRWAGDDPDREGLVGTPDRVVRAYEEFFSGYD
QDPTEILHRTFEETDGYDEMVLLRDIRLESHCEHHVVPIIGKAHVAYLPNRRVVGISKLARVVEIYSRRLQIQEKLTAQI
ANTINDVLQPKGVAVVIEAAHQCMTTRGIHKPGVTMVTSRMLGAFREDPATRREFMALIHNQHSGGLSNV

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=203, Percent_Identity=48.2758620689655, Blast_Score=199, Evalue=1e-51,
Organism=Homo sapiens, GI4503949, Length=203, Percent_Identity=48.2758620689655, Blast_Score=199, Evalue=1e-51,
Organism=Homo sapiens, GI66932970, Length=171, Percent_Identity=44.4444444444444, Blast_Score=153, Evalue=1e-37,
Organism=Homo sapiens, GI66932972, Length=161, Percent_Identity=46.583850931677, Blast_Score=150, Evalue=7e-37,
Organism=Escherichia coli, GI1788476, Length=172, Percent_Identity=39.5348837209302, Blast_Score=117, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17560486, Length=177, Percent_Identity=49.7175141242938, Blast_Score=197, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6321706, Length=178, Percent_Identity=51.123595505618, Blast_Score=186, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24656782, Length=191, Percent_Identity=47.6439790575916, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24656796, Length=191, Percent_Identity=47.6439790575916, Blast_Score=178, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24656791, Length=191, Percent_Identity=47.6439790575916, Blast_Score=178, Evalue=3e-45,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 26096; Mature: 25965

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDRDHRERKAAMSKDATSLTLPRELLPATGSRLDKPSREEAEEAVRTLLRWAGDDPDRE
CCCHHHHHHHHHHHCCCCHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
GLVGTPDRVVRAYEEFFSGYDQDPTEILHRTFEETDGYDEMVLLRDIRLESHCEHHVVPI
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEE
IGKAHVAYLPNRRVVGISKLARVVEIYSRRLQIQEKLTAQIANTINDVLQPKGVAVVIEA
ECCHHEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AHQCMTTRGIHKPGVTMVTSRMLGAFREDPATRREFMALIHNQHSGGLSNV
HHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PDRDHRERKAAMSKDATSLTLPRELLPATGSRLDKPSREEAEEAVRTLLRWAGDDPDRE
CCHHHHHHHHHHHCCCCHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
GLVGTPDRVVRAYEEFFSGYDQDPTEILHRTFEETDGYDEMVLLRDIRLESHCEHHVVPI
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEE
IGKAHVAYLPNRRVVGISKLARVVEIYSRRLQIQEKLTAQIANTINDVLQPKGVAVVIEA
ECCHHEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AHQCMTTRGIHKPGVTMVTSRMLGAFREDPATRREFMALIHNQHSGGLSNV
HHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]