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The map label for this gene is gcvA [H]

Identifier: 83313249

GI number: 83313249

Start: 4558736

End: 4559629

Strand: Reverse

Name: gcvA [H]

Synonym: amb4150

Alternate gene names: 83313249

Gene position: 4559629-4558736 (Counterclockwise)

Preceding gene: 83313250

Following gene: 83313248

Centisome position: 91.8

GC content: 68.57

Gene sequence:

>894_bases
ATGCCGTTTCGCCTGCCCCCCCTTTCCTCGCTTCGCTTCTTCGAAGCGGCCGGAAGGCATCGGAGTTTCAAGCGCGCCGC
CCAGGAGCTGAACGTCACGGCCAGCGCGGTCAGCCACGGTATCGTGGCGCTGGAAAAGGCGCTGGGCGTCGCGTTGTTCG
TCCGCGAGACCCGAAGCCTGTCGCTGACCCCGGAGGGGGAGGGCTACCTGCCCTATATCTCCGAGGCTCTGTCGCTGATC
GCCATTGGAACGCAGCGCCTGCCGGGCGGTCCGGGAGAGCGGGGCATCTCGGTGAGTTGCGCCCCGACTATCGCCTCGCG
CTGGCTCCTGCCGCGGCTTCCCGAATTCAGCCGGCGCTGGCCCCAGCTGTCCGTCTCCGTCGACACCTCTCACCGTCAGG
TCAGTTTTCCGGTGGACGGATTCGACTTCGCCATCCGCATGAGCCGTATTCCTTTGGCCGGGGCGGCCTGGACGCGGCTG
TTCTCGGAGCGATTGGTTCCGGTCTGCTCGCCCGCCTATGCCGCGAGCCTGTCCGCCGCCGGGGGAGGTCCGGGGCTTTG
TGGCGCGACGCTGATCCATGTGGTCTCGGTCAGCGAGGACTGGTCCGCCTGGGCCGACGCCACGGGGACACGGGGGCTGG
ACCTCGCCGGCGGCATTCGCATCGATGCCATCCAGTTGGCCTTCGAGGCCGCCCGGGCCGGGCTGGGGGTGGCGCTGGGG
CGCAAGCCTCTGGTAGACCTCGAACTGGCCGACGGCTCGTTGGTGGAGGTGGGGCCGGAGGTTGCCGCCACCGAATCCGC
CTACTGGCTGGTCTGTTCCGATGCCGTCGAGCACCGGCCCGATCTCCGCAATTTCAAGCGCTGGCTGATCGCCCAGGTGG
ACTCTGCCGGATAG

Upstream 100 bases:

>100_bases
GCGGCGGTCCTTGCTGTCCCGCCGCGCCATACGGTGGTGTCGGCCGGTTGATGCCGCCCGGGTTGCCCGGTAGGCTCTGG
GGATCGGAGGAGAGGCCATC

Downstream 100 bases:

>100_bases
ATGAGGAGTCCTCATCCATCCGCGAAGGCTTCTCGTTTGCCGCCGTCATGGCCGCTTTCCATTCTGAGGGTTCAGGATGG
AGGTCGAGATGAAGACAAAC

Product: transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MPFRLPPLSSLRFFEAAGRHRSFKRAAQELNVTASAVSHGIVALEKALGVALFVRETRSLSLTPEGEGYLPYISEALSLI
AIGTQRLPGGPGERGISVSCAPTIASRWLLPRLPEFSRRWPQLSVSVDTSHRQVSFPVDGFDFAIRMSRIPLAGAAWTRL
FSERLVPVCSPAYAASLSAAGGGPGLCGATLIHVVSVSEDWSAWADATGTRGLDLAGGIRIDAIQLAFEAARAGLGVALG
RKPLVDLELADGSLVEVGPEVAATESAYWLVCSDAVEHRPDLRNFKRWLIAQVDSAG

Sequences:

>Translated_297_residues
MPFRLPPLSSLRFFEAAGRHRSFKRAAQELNVTASAVSHGIVALEKALGVALFVRETRSLSLTPEGEGYLPYISEALSLI
AIGTQRLPGGPGERGISVSCAPTIASRWLLPRLPEFSRRWPQLSVSVDTSHRQVSFPVDGFDFAIRMSRIPLAGAAWTRL
FSERLVPVCSPAYAASLSAAGGGPGLCGATLIHVVSVSEDWSAWADATGTRGLDLAGGIRIDAIQLAFEAARAGLGVALG
RKPLVDLELADGSLVEVGPEVAATESAYWLVCSDAVEHRPDLRNFKRWLIAQVDSAG
>Mature_296_residues
PFRLPPLSSLRFFEAAGRHRSFKRAAQELNVTASAVSHGIVALEKALGVALFVRETRSLSLTPEGEGYLPYISEALSLIA
IGTQRLPGGPGERGISVSCAPTIASRWLLPRLPEFSRRWPQLSVSVDTSHRQVSFPVDGFDFAIRMSRIPLAGAAWTRLF
SERLVPVCSPAYAASLSAAGGGPGLCGATLIHVVSVSEDWSAWADATGTRGLDLAGGIRIDAIQLAFEAARAGLGVALGR
KPLVDLELADGSLVEVGPEVAATESAYWLVCSDAVEHRPDLRNFKRWLIAQVDSAG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=292, Percent_Identity=35.6164383561644, Blast_Score=174, Evalue=6e-45,
Organism=Escherichia coli, GI1788706, Length=301, Percent_Identity=34.21926910299, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1786448, Length=300, Percent_Identity=34.6666666666667, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI145693193, Length=289, Percent_Identity=27.3356401384083, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786401, Length=246, Percent_Identity=27.2357723577236, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787128, Length=149, Percent_Identity=32.2147651006711, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI145693105, Length=199, Percent_Identity=28.643216080402, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790262, Length=189, Percent_Identity=26.984126984127, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI157672245, Length=120, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081978, Length=263, Percent_Identity=24.7148288973384, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31670; Mature: 31538

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFRLPPLSSLRFFEAAGRHRSFKRAAQELNVTASAVSHGIVALEKALGVALFVRETRSL
CCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHEEEEECCCCE
SLTPEGEGYLPYISEALSLIAIGTQRLPGGPGERGISVSCAPTIASRWLLPRLPEFSRRW
EECCCCCCCCHHHHHHHHHHHHCHHCCCCCCCCCCEEEEECHHHHHHHHCCCCCHHHHCC
PQLSVSVDTSHRQVSFPVDGFDFAIRMSRIPLAGAAWTRLFSERLVPVCSPAYAASLSAA
CEEEEEEECCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCC
GGGPGLCGATLIHVVSVSEDWSAWADATGTRGLDLAGGIRIDAIQLAFEAARAGLGVALG
CCCCCHHHHHHHHHEECCCCHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCEECC
RKPLVDLELADGSLVEVGPEVAATESAYWLVCSDAVEHRPDLRNFKRWLIAQVDSAG
CCCCEEEEECCCCEEECCCCCEECCCEEEEEECCHHHCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PFRLPPLSSLRFFEAAGRHRSFKRAAQELNVTASAVSHGIVALEKALGVALFVRETRSL
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHEEEEECCCCE
SLTPEGEGYLPYISEALSLIAIGTQRLPGGPGERGISVSCAPTIASRWLLPRLPEFSRRW
EECCCCCCCCHHHHHHHHHHHHCHHCCCCCCCCCCEEEEECHHHHHHHHCCCCCHHHHCC
PQLSVSVDTSHRQVSFPVDGFDFAIRMSRIPLAGAAWTRLFSERLVPVCSPAYAASLSAA
CEEEEEEECCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCC
GGGPGLCGATLIHVVSVSEDWSAWADATGTRGLDLAGGIRIDAIQLAFEAARAGLGVALG
CCCCCHHHHHHHHHEECCCCHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCEECC
RKPLVDLELADGSLVEVGPEVAATESAYWLVCSDAVEHRPDLRNFKRWLIAQVDSAG
CCCCEEEEECCCCEEECCCCCEECCCEEEEEECCHHHCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]