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The map label for this gene is 83313212

Identifier: 83313212

GI number: 83313212

Start: 4517104

End: 4518642

Strand: Reverse

Name: 83313212

Synonym: amb4113

Alternate gene names: NA

Gene position: 4518642-4517104 (Counterclockwise)

Preceding gene: 83313219

Following gene: 83313211

Centisome position: 90.97

GC content: 68.16

Gene sequence:

>1539_bases
ATGGTCTATCGTTCCCTCACCCGTCGATACCAAGTGAGTCCCATGAGCACCAATCCGGCCTATGCCACCCTGGAAGCCCG
CTTCCGCAAGATGAACTCCGTCGCCGAGGCGCTGTCGGTGCTGTCCTGGGACATGGCGACCATGATGCCCGAAGGTGCCG
CCGAGATGCGGGCCGAGCAGGTGGCGACCCTGCGCGGCATCAATCACGAACAGATCACCGCGCCGGACATGGGCGAATTG
CTCGATCGCGCGGCGGCCGAGCCGCTGGATGCCTGGCAGGCGGCCAATCTGCGCGAGATGCGCCGCGACTGGATTCATGC
CAGCGCTCTGCCCGCCGATCTGGTGGAGGCCCAGGCGCGGGCCGAATCCGCCTGCGAGATGGTCTGGCGCCAGGCCCGCC
CGGCGGGGGATTTCAAGGCGGTGCTGCCCGCCCTGAAGGTGCTGCTGGGGCTGGTGCGCGAGGTGGGGCACGTCAAGGCC
GAGGCCCTGGGCGTGTCCATCTACGACGCCCTGCTCGACCAGTACGAGCCGCTGGGCCGTGCGGCGGAGATCGATCCGGT
CTTCGACCACCTGGAGACGTTCCTGCCCGGCTTCATCGGTGAGGTTCTGGACGCCCAGAACGCCCGCCCGCCGATCATCG
AGCCGGACGGCCCCTTCGCCACCGACCGCCAAAAGGCTCTCGGCATCCGTCTGATGGAGGTGCTGGGCTTCGACTTCCGC
CACGGTCGCCTGGACGTGTCCCATCACCCGTTCTGCGGCGGCTATCCCGGCGATATCCGGGTCACCACCCGTTATAACGA
GGACGATTTCGCCTCGGCCATGATGGGGGTGCTGCACGAGACCGGCCACGCGCTCTACGAGTTCGGCCTGCCGTCCGGCC
ATTGGCGCCCTCAGCCGGTGGGCCGGGCGCGCGGCATGGTGGTGCACGAATCCCAATCGCTGCTCATGGAGATGCAGGTC
TGCCGCTCCAAGGAGTTCGCCGGCTTCCTCTCGCCGCTGCTGAAAAGCACGTTCGGCGCCGAGGGGCCGGCCTGGGAGCC
CGACAACATCTACCGCCGGGGCATCAAGGTCAGCCGGGGGTTCATCCGGGTCGAGGCCGACGAGGTCACCTATCCCGCCC
ACGTCATCATCCGCTATCGCCTGGAAAAGGCGCTGATCGAAGGCCGGATGGAGTTGGAGGACCTGCCCGCCGCCTGGAAC
GAGGGCTATCAGCGCCTGCTGGGCATCACGCCGCCCGACGACCGGCTGGGCTGCCTGCAGGACATTCACTGGTATGGCGG
CGCCTGGGGCTATTTCCCCACCTATACGCTGGGCGCCATGACCGCCGCCCAATTGTTCGACGCCGCCTGCCACGCCGATC
CGGCGGTGATGAAGGGTCTGGCCGGGGGCGACTTCCGGCCGCTGCTCGGCTGGCTGCGGGCCAACGTCCATTCCAAGGGC
TCGCTGCTGTCCACCCGGCAGTTGATGACCCAGGCGACGGCGCGGCCGCTCGATCCCACGGTGTTCGAGACCCATCTGCG
GCGGCGCTACCTGGGCTGA

Upstream 100 bases:

>100_bases
GACAGGACGACGGCGAAGCGGGGTTTGGCGGTCATGGCGTGTTCCTTATCCTCGGCTGGGGAAAACTCGGGCGATTATAC
GATTCCCCGAAGCAAGGGCC

Downstream 100 bases:

>100_bases
GGGATTTCGGGTAGCATTGGATCATGCTCGGCTGGCTGTCCCGTCTGGTTCTGGACAAACGGGCGCGCGAGGCGCTCAAG
CGCCCGGCGCCCAAGGCGGC

Product: thermostable carboxypeptidase 1

Products: NA

Alternate protein names: Carboxypeptidase Taq [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MVYRSLTRRYQVSPMSTNPAYATLEARFRKMNSVAEALSVLSWDMATMMPEGAAEMRAEQVATLRGINHEQITAPDMGEL
LDRAAAEPLDAWQAANLREMRRDWIHASALPADLVEAQARAESACEMVWRQARPAGDFKAVLPALKVLLGLVREVGHVKA
EALGVSIYDALLDQYEPLGRAAEIDPVFDHLETFLPGFIGEVLDAQNARPPIIEPDGPFATDRQKALGIRLMEVLGFDFR
HGRLDVSHHPFCGGYPGDIRVTTRYNEDDFASAMMGVLHETGHALYEFGLPSGHWRPQPVGRARGMVVHESQSLLMEMQV
CRSKEFAGFLSPLLKSTFGAEGPAWEPDNIYRRGIKVSRGFIRVEADEVTYPAHVIIRYRLEKALIEGRMELEDLPAAWN
EGYQRLLGITPPDDRLGCLQDIHWYGGAWGYFPTYTLGAMTAAQLFDAACHADPAVMKGLAGGDFRPLLGWLRANVHSKG
SLLSTRQLMTQATARPLDPTVFETHLRRRYLG

Sequences:

>Translated_512_residues
MVYRSLTRRYQVSPMSTNPAYATLEARFRKMNSVAEALSVLSWDMATMMPEGAAEMRAEQVATLRGINHEQITAPDMGEL
LDRAAAEPLDAWQAANLREMRRDWIHASALPADLVEAQARAESACEMVWRQARPAGDFKAVLPALKVLLGLVREVGHVKA
EALGVSIYDALLDQYEPLGRAAEIDPVFDHLETFLPGFIGEVLDAQNARPPIIEPDGPFATDRQKALGIRLMEVLGFDFR
HGRLDVSHHPFCGGYPGDIRVTTRYNEDDFASAMMGVLHETGHALYEFGLPSGHWRPQPVGRARGMVVHESQSLLMEMQV
CRSKEFAGFLSPLLKSTFGAEGPAWEPDNIYRRGIKVSRGFIRVEADEVTYPAHVIIRYRLEKALIEGRMELEDLPAAWN
EGYQRLLGITPPDDRLGCLQDIHWYGGAWGYFPTYTLGAMTAAQLFDAACHADPAVMKGLAGGDFRPLLGWLRANVHSKG
SLLSTRQLMTQATARPLDPTVFETHLRRRYLG
>Mature_512_residues
MVYRSLTRRYQVSPMSTNPAYATLEARFRKMNSVAEALSVLSWDMATMMPEGAAEMRAEQVATLRGINHEQITAPDMGEL
LDRAAAEPLDAWQAANLREMRRDWIHASALPADLVEAQARAESACEMVWRQARPAGDFKAVLPALKVLLGLVREVGHVKA
EALGVSIYDALLDQYEPLGRAAEIDPVFDHLETFLPGFIGEVLDAQNARPPIIEPDGPFATDRQKALGIRLMEVLGFDFR
HGRLDVSHHPFCGGYPGDIRVTTRYNEDDFASAMMGVLHETGHALYEFGLPSGHWRPQPVGRARGMVVHESQSLLMEMQV
CRSKEFAGFLSPLLKSTFGAEGPAWEPDNIYRRGIKVSRGFIRVEADEVTYPAHVIIRYRLEKALIEGRMELEDLPAAWN
EGYQRLLGITPPDDRLGCLQDIHWYGGAWGYFPTYTLGAMTAAQLFDAACHADPAVMKGLAGGDFRPLLGWLRANVHSKG
SLLSTRQLMTQATARPLDPTVFETHLRRRYLG

Specific function: Releases amino acids sequentially from the C-terminus with a broad substrate specificity (except for proline) [H]

COG id: COG2317

COG function: function code E; Zn-dependent carboxypeptidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M32 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001333 [H]

Pfam domain/function: PF02074 Peptidase_M32 [H]

EC number: =3.4.17.19 [H]

Molecular weight: Translated: 57066; Mature: 57066

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYRSLTRRYQVSPMSTNPAYATLEARFRKMNSVAEALSVLSWDMATMMPEGAAEMRAEQ
CCCHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VATLRGINHEQITAPDMGELLDRAAAEPLDAWQAANLREMRRDWIHASALPADLVEAQAR
HHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHH
AESACEMVWRQARPAGDFKAVLPALKVLLGLVREVGHVKAEALGVSIYDALLDQYEPLGR
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AAEIDPVFDHLETFLPGFIGEVLDAQNARPPIIEPDGPFATDRQKALGIRLMEVLGFDFR
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCCC
HGRLDVSHHPFCGGYPGDIRVTTRYNEDDFASAMMGVLHETGHALYEFGLPSGHWRPQPV
CCEECCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCHHHEECCCCCCCCCCCCC
GRARGMVVHESQSLLMEMQVCRSKEFAGFLSPLLKSTFGAEGPAWEPDNIYRRGIKVSRG
HHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCHHEECC
FIRVEADEVTYPAHVIIRYRLEKALIEGRMELEDLPAAWNEGYQRLLGITPPDDRLGCLQ
EEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHH
DIHWYGGAWGYFPTYTLGAMTAAQLFDAACHADPAVMKGLAGGDFRPLLGWLRANVHSKG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHCCCCCC
SLLSTRQLMTQATARPLDPTVFETHLRRRYLG
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MVYRSLTRRYQVSPMSTNPAYATLEARFRKMNSVAEALSVLSWDMATMMPEGAAEMRAEQ
CCCHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VATLRGINHEQITAPDMGELLDRAAAEPLDAWQAANLREMRRDWIHASALPADLVEAQAR
HHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHH
AESACEMVWRQARPAGDFKAVLPALKVLLGLVREVGHVKAEALGVSIYDALLDQYEPLGR
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AAEIDPVFDHLETFLPGFIGEVLDAQNARPPIIEPDGPFATDRQKALGIRLMEVLGFDFR
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCCC
HGRLDVSHHPFCGGYPGDIRVTTRYNEDDFASAMMGVLHETGHALYEFGLPSGHWRPQPV
CCEECCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCHHHEECCCCCCCCCCCCC
GRARGMVVHESQSLLMEMQVCRSKEFAGFLSPLLKSTFGAEGPAWEPDNIYRRGIKVSRG
HHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCHHEECC
FIRVEADEVTYPAHVIIRYRLEKALIEGRMELEDLPAAWNEGYQRLLGITPPDDRLGCLQ
EEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHH
DIHWYGGAWGYFPTYTLGAMTAAQLFDAACHADPAVMKGLAGGDFRPLLGWLRANVHSKG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHCCCCCC
SLLSTRQLMTQATARPLDPTVFETHLRRRYLG
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7765282; 1369078 [H]