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The map label for this gene is mcl1 [H]

Identifier: 83313076

GI number: 83313076

Start: 4387898

End: 4389043

Strand: Reverse

Name: mcl1 [H]

Synonym: amb3977

Alternate gene names: 83313076

Gene position: 4389043-4387898 (Counterclockwise)

Preceding gene: 83313080

Following gene: 83313071

Centisome position: 88.36

GC content: 66.4

Gene sequence:

>1146_bases
ATGACGGTGTGCCCGTGGAGCTTTCCCCTTGTGCACCGCAGCGCGACGAAGCATTATTGCGGCCCATCGTCTTTCGCCGC
AAGGAGATTGCGCCTCATGTCCGTCAAGCCGCCCCGCAAGTTCTTCGAGCCGCTCGCCATCGGCGCGCCCGCGCCCTATC
GCGAACTGCCGGTGCGCCTCGAGCGCATGATTCACTTCTTCCCGCCGCACAACGAGAAGATGCGGGCCAAGGCCGCCGAG
ATGGGCAAGAACGTCGACGTTCTGCTGGGCAATCTGGAAGACGCCATTCCCGCCGATGCCAAGGACGCGGCCCGCGCCGG
CTTCGTCGAGGTGGCCAAGGCCTGGGACAATCCCAATACCGGCCTGTGGACCCGCGTCAACTGCCTGAACTCGCCCTGGT
TCCTCGACGACATGAACGCCATCGTCGGCGAGGCCGGCAACAAGGTGGACGTGGTGATGCTGCCCAAGGTGGAAGGCCCC
TGGGACATCCATTACCTGGACCAGCTGCTGGCCCAGCTGGAGGCCAAGCACGGCGTCAAGCGCCCGATCATGATCCACGC
TATCCTCGAGACGGCGCTGGGCGTGGAGAACGTGGCCGCCATCTGTCAGGCCAGCCCGCGCATGCACGGCATCAGCCTCG
GCCCGGCCGATCTGGCGGCGTCGCGCGGCATGAAGACCACCCGCGTGGGCGGCGGCCATCCCTTCTATGGCGTGCTGGAA
GACGCAGCCGAGGGCAAGGCGGGCCGCACCCTGTTCCAGCAGGATCTGTGGCACTACACCGTGGCCAAGATGGTCGATGC
CTGCCAGTCGGCGGGGATCAAGGCCTTCTACGGCCCGTTCGGCGATTTCTCCGACGACGCGGCCTGCGAGGCTCAGTTCC
GCAACGCCTTCCTGCTGGGCTGCGCCGGCGGCTGGTCGCTGCACCCCAAGCAGATCGACATCGCCAAGAAGGTGTTCAGC
CCCGACGTGGCCGAAGTGCTGTTCGCCAAGAAGATCCTCGAAGCCATGCCCGACGGCACCGGCGCCGTGATGATCGACGG
CAAGATGCAGGACGACGCCACCTGGAAGCAGGCCAAGGTCATGGTCGACCTGGCCAAGGCGGTGGCCGCCAAGGACCCGG
CGATGGCCCAGGCCTACGGGCTGTAA

Upstream 100 bases:

>100_bases
TGATTCATGGCGGCCCCTGTCATCAGCTCTCACCCCATCCCATGGTATAAGCCCGAAGCCCGGGGGGGAATACCCGAAAT
GTGGCCGTGGGCGACCAATT

Downstream 100 bases:

>100_bases
GACTTAGGGAGCCCCATGCGCGGGGCTCCCGCCCCCCAATCTCGGAGAGATGAGCCCCGGTTGGAGGCGATGCCTCCAAG
CCTCCCTTTGAATCAAGAGG

Product: citrate lyase beta subunit

Products: NA

Alternate protein names: (3S)-malyl-CoA/beta-methylmalyl-CoA lyase; L-malyl-CoA/beta-methylmalyl-CoA lyase [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MTVCPWSFPLVHRSATKHYCGPSSFAARRLRLMSVKPPRKFFEPLAIGAPAPYRELPVRLERMIHFFPPHNEKMRAKAAE
MGKNVDVLLGNLEDAIPADAKDAARAGFVEVAKAWDNPNTGLWTRVNCLNSPWFLDDMNAIVGEAGNKVDVVMLPKVEGP
WDIHYLDQLLAQLEAKHGVKRPIMIHAILETALGVENVAAICQASPRMHGISLGPADLAASRGMKTTRVGGGHPFYGVLE
DAAEGKAGRTLFQQDLWHYTVAKMVDACQSAGIKAFYGPFGDFSDDAACEAQFRNAFLLGCAGGWSLHPKQIDIAKKVFS
PDVAEVLFAKKILEAMPDGTGAVMIDGKMQDDATWKQAKVMVDLAKAVAAKDPAMAQAYGL

Sequences:

>Translated_381_residues
MTVCPWSFPLVHRSATKHYCGPSSFAARRLRLMSVKPPRKFFEPLAIGAPAPYRELPVRLERMIHFFPPHNEKMRAKAAE
MGKNVDVLLGNLEDAIPADAKDAARAGFVEVAKAWDNPNTGLWTRVNCLNSPWFLDDMNAIVGEAGNKVDVVMLPKVEGP
WDIHYLDQLLAQLEAKHGVKRPIMIHAILETALGVENVAAICQASPRMHGISLGPADLAASRGMKTTRVGGGHPFYGVLE
DAAEGKAGRTLFQQDLWHYTVAKMVDACQSAGIKAFYGPFGDFSDDAACEAQFRNAFLLGCAGGWSLHPKQIDIAKKVFS
PDVAEVLFAKKILEAMPDGTGAVMIDGKMQDDATWKQAKVMVDLAKAVAAKDPAMAQAYGL
>Mature_380_residues
TVCPWSFPLVHRSATKHYCGPSSFAARRLRLMSVKPPRKFFEPLAIGAPAPYRELPVRLERMIHFFPPHNEKMRAKAAEM
GKNVDVLLGNLEDAIPADAKDAARAGFVEVAKAWDNPNTGLWTRVNCLNSPWFLDDMNAIVGEAGNKVDVVMLPKVEGPW
DIHYLDQLLAQLEAKHGVKRPIMIHAILETALGVENVAAICQASPRMHGISLGPADLAASRGMKTTRVGGGHPFYGVLED
AAEGKAGRTLFQQDLWHYTVAKMVDACQSAGIKAFYGPFGDFSDDAACEAQFRNAFLLGCAGGWSLHPKQIDIAKKVFSP
DVAEVLFAKKILEAMPDGTGAVMIDGKMQDDATWKQAKVMVDLAKAVAAKDPAMAQAYGL

Specific function: Catalyzes the reversible condensation of glyoxylate and acetyl-CoA to L-malyl-CoA and the reversible condensation of glyoxylate and propionyl-CoA to beta-methylmalyl-CoA [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=323, Percent_Identity=25.6965944272446, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI87081764, Length=325, Percent_Identity=25.8461538461538, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.3.24 [H]

Molecular weight: Translated: 41480; Mature: 41349

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVCPWSFPLVHRSATKHYCGPSSFAARRLRLMSVKPPRKFFEPLAIGAPAPYRELPVRL
CCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHH
ERMIHFFPPHNEKMRAKAAEMGKNVDVLLGNLEDAIPADAKDAARAGFVEVAKAWDNPNT
HHHHHHCCCCCHHHHHHHHHHCCCCEEEECCHHHCCCCCCHHHHHHHHHHHHHHCCCCCC
GLWTRVNCLNSPWFLDDMNAIVGEAGNKVDVVMLPKVEGPWDIHYLDQLLAQLEAKHGVK
CCEEEEEECCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCC
RPIMIHAILETALGVENVAAICQASPRMHGISLGPADLAASRGMKTTRVGGGHPFYGVLE
CCHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCEEECCCCCCCHHHHH
DAAEGKAGRTLFQQDLWHYTVAKMVDACQSAGIKAFYGPFGDFSDDAACEAQFRNAFLLG
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHCCEEEEE
CAGGWSLHPKQIDIAKKVFSPDVAEVLFAKKILEAMPDGTGAVMIDGKMQDDATWKQAKV
ECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHH
MVDLAKAVAAKDPAMAQAYGL
HHHHHHHHHCCCCHHHHHCCC
>Mature Secondary Structure 
TVCPWSFPLVHRSATKHYCGPSSFAARRLRLMSVKPPRKFFEPLAIGAPAPYRELPVRL
CCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHH
ERMIHFFPPHNEKMRAKAAEMGKNVDVLLGNLEDAIPADAKDAARAGFVEVAKAWDNPNT
HHHHHHCCCCCHHHHHHHHHHCCCCEEEECCHHHCCCCCCHHHHHHHHHHHHHHCCCCCC
GLWTRVNCLNSPWFLDDMNAIVGEAGNKVDVVMLPKVEGPWDIHYLDQLLAQLEAKHGVK
CCEEEEEECCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCC
RPIMIHAILETALGVENVAAICQASPRMHGISLGPADLAASRGMKTTRVGGGHPFYGVLE
CCHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCEEECCCCCCCHHHHH
DAAEGKAGRTLFQQDLWHYTVAKMVDACQSAGIKAFYGPFGDFSDDAACEAQFRNAFLLG
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHCCEEEEE
CAGGWSLHPKQIDIAKKVFSPDVAEVLFAKKILEAMPDGTGAVMIDGKMQDDATWKQAKV
ECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHH
MVDLAKAVAAKDPAMAQAYGL
HHHHHHHHHCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA