Click here to switch to the map view.

The map label for this gene is phbC [H]

Identifier: 83312824

GI number: 83312824

Start: 4096987

End: 4098783

Strand: Reverse

Name: phbC [H]

Synonym: amb3725

Alternate gene names: 83312824

Gene position: 4098783-4096987 (Counterclockwise)

Preceding gene: 83312830

Following gene: 83312821

Centisome position: 82.52

GC content: 62.99

Gene sequence:

>1797_bases
ATGGCCGAGCAGACGGGCACCGAGTCCAAGATGATCGACGCGACGGAACTTTCCAAGGCGATGACCAGCATTGCGGAACG
CTCTCAGCGCATCGTCGCTGATTTTCTGACCCGCCAGGCGGCGGACCCCACCGCGGCGGCGGCCGACCCCTTGAATATCG
GCAACGCCTTCATGGAAATGACGGCCAAGCTGATGAGCGACCCGGCCAAGCTGGTCGAGGCCAATCTCAACCTGTGGCAG
GATTACATGTCGCTGTGGCAGAACACCGCCCGGCGCATGCTGGGGGAGGAGACCCAGCCGGTGGTCGCCCCCGATTCCGG
TGATCGCCGCTTCAAGGACGAGATGTGGCAGGAGAATGAAATCTTCGACTTCATCAAGCAGTCCTATCTGCTGTCCGCCC
GCTGGATGCAGGGCGTGGTGAAGAATGTCGAGGGTCTGGACGACCACACCGCCAAGAAGGTCGACTTCTATACCCGCCAG
TTCGTGGACGCCATGGCGCCCAGCAATTTCGTCCTGACCAACCCCGAGGTGCTGCGCGCCACGGTGGAGACCCGGGGCGA
AAACCTGCTGAAGGGCCTGAATAACCTGCTGGATGACCTGGAGCGCGGCAAGGGCAACCTGGCCATCAAGATGACCGATT
ACGACGCCTTCAAGGTGGGCGAGAACATCGCCGTCACCCCGGGCAAGGTGGTCTTCCAGACCGATTTGATGCAGCTGATC
CAGTACGATCCGACCACGCCACAGGCGTTCGAGAAGCCCTTGCTGATCCTGCCGCCGTGGATCAACAAGTTCTACATCCT
GGACCTGCGCCCCAAGAACTCGCTGATCAAGTGGGCGGTGGACCAGGGCCACACGGTGTTCGTCATTTCCTGGGTCAATC
CGGACGAGAAGCTGGCCCACAAGGGCTTCGGCGACTACATGCTGGAAGGCCCGCTGGCCGCCATCGACGCCATCGAGAAG
GCGACCGGCTCGCGTCAGGTCAATGCGGCCGGCTATTGCCTGGGCGGCACGCTGTTGGCCTGCACCCTGGCCTATCTGGC
CGCCAAGGGCGAGGACCGCATCGCCTCGGCCACCTTCTTCACCACCATGACCGACTTCAAGGACCCCGGCGAACTGGGCG
TCTTCATCGACGAGGAGCAGCTCTCGGCCCTGGAAACCCGCATGAACCAGGCGGGCTTCCTGGACGGCCGCGACATGGCC
ACCACCTTCAACATGATGCGCGCCAACGACCTGATCTGGTCGTTCGTGGTCAATAATTACCTGCTGGGCAAGGACCCGTT
CCCGTTCGACCTGCTGTACTGGAACTCGGATTCGACCCGCATGCCGGCGGCCATGCACAGCTTCTACCTGCGCAAGATGT
ACCAGGAGAACCGGCTGGTCGAGCCGGGCGCCATCGAGCTGAACGGCGTCAAGATCGACCTCGGCAACATCAAGACCCCG
CTCTACATGCTATCGACGCGCGAGGATCACATCGCGCCGTGGCAGAGCACTTATGCCCTGACCCAGAACGTGTCCGGCCC
CATCAAGTTCGTGCTGTCGGCCTCGGGCCACATCGCCGGCGTGGTCAATCCGCCCGCCGCCAACAAGTACTGCTACTGGA
CCAATGCCAAGAAGCCCAAGAACCCCGAAGTCTGGCTGGCCGGTGCCACCCAGGTGGAGGGCTCGTGGTGGACCGATTGG
CAGAAGTGGGTCGAGAAGTTCGCCGGCAAGCAGGTTCCCGCCCGCCAGCCCGGCGACGGCAAGCTGAAGGCCATCGAGGC
GGCCCCCGGCTCCTACGTCCAGGTCAAGGCGGTGTAA

Upstream 100 bases:

>100_bases
ATGGGCGGTGCACCCACCGGAACGTCGGGCGACGCGACACACAGTCAATCGCCGTGTATGGTTGCACTCTGGATGCACCG
TAAAATGTAAGGGGTAGCTG

Downstream 100 bases:

>100_bases
ATCACCATAGCTGACCGGTCGGTCGATCAAGGCGGCTCCTGCGGGGGCCGCCTTTTTTTGAGACAGGGGTGACCGCTGGG
GGCTGCGGAAAAGCTCCACT

Product: Poly(3-hydroxyalkanoate) synthetase

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MAEQTGTESKMIDATELSKAMTSIAERSQRIVADFLTRQAADPTAAAADPLNIGNAFMEMTAKLMSDPAKLVEANLNLWQ
DYMSLWQNTARRMLGEETQPVVAPDSGDRRFKDEMWQENEIFDFIKQSYLLSARWMQGVVKNVEGLDDHTAKKVDFYTRQ
FVDAMAPSNFVLTNPEVLRATVETRGENLLKGLNNLLDDLERGKGNLAIKMTDYDAFKVGENIAVTPGKVVFQTDLMQLI
QYDPTTPQAFEKPLLILPPWINKFYILDLRPKNSLIKWAVDQGHTVFVISWVNPDEKLAHKGFGDYMLEGPLAAIDAIEK
ATGSRQVNAAGYCLGGTLLACTLAYLAAKGEDRIASATFFTTMTDFKDPGELGVFIDEEQLSALETRMNQAGFLDGRDMA
TTFNMMRANDLIWSFVVNNYLLGKDPFPFDLLYWNSDSTRMPAAMHSFYLRKMYQENRLVEPGAIELNGVKIDLGNIKTP
LYMLSTREDHIAPWQSTYALTQNVSGPIKFVLSASGHIAGVVNPPAANKYCYWTNAKKPKNPEVWLAGATQVEGSWWTDW
QKWVEKFAGKQVPARQPGDGKLKAIEAAPGSYVQVKAV

Sequences:

>Translated_598_residues
MAEQTGTESKMIDATELSKAMTSIAERSQRIVADFLTRQAADPTAAAADPLNIGNAFMEMTAKLMSDPAKLVEANLNLWQ
DYMSLWQNTARRMLGEETQPVVAPDSGDRRFKDEMWQENEIFDFIKQSYLLSARWMQGVVKNVEGLDDHTAKKVDFYTRQ
FVDAMAPSNFVLTNPEVLRATVETRGENLLKGLNNLLDDLERGKGNLAIKMTDYDAFKVGENIAVTPGKVVFQTDLMQLI
QYDPTTPQAFEKPLLILPPWINKFYILDLRPKNSLIKWAVDQGHTVFVISWVNPDEKLAHKGFGDYMLEGPLAAIDAIEK
ATGSRQVNAAGYCLGGTLLACTLAYLAAKGEDRIASATFFTTMTDFKDPGELGVFIDEEQLSALETRMNQAGFLDGRDMA
TTFNMMRANDLIWSFVVNNYLLGKDPFPFDLLYWNSDSTRMPAAMHSFYLRKMYQENRLVEPGAIELNGVKIDLGNIKTP
LYMLSTREDHIAPWQSTYALTQNVSGPIKFVLSASGHIAGVVNPPAANKYCYWTNAKKPKNPEVWLAGATQVEGSWWTDW
QKWVEKFAGKQVPARQPGDGKLKAIEAAPGSYVQVKAV
>Mature_597_residues
AEQTGTESKMIDATELSKAMTSIAERSQRIVADFLTRQAADPTAAAADPLNIGNAFMEMTAKLMSDPAKLVEANLNLWQD
YMSLWQNTARRMLGEETQPVVAPDSGDRRFKDEMWQENEIFDFIKQSYLLSARWMQGVVKNVEGLDDHTAKKVDFYTRQF
VDAMAPSNFVLTNPEVLRATVETRGENLLKGLNNLLDDLERGKGNLAIKMTDYDAFKVGENIAVTPGKVVFQTDLMQLIQ
YDPTTPQAFEKPLLILPPWINKFYILDLRPKNSLIKWAVDQGHTVFVISWVNPDEKLAHKGFGDYMLEGPLAAIDAIEKA
TGSRQVNAAGYCLGGTLLACTLAYLAAKGEDRIASATFFTTMTDFKDPGELGVFIDEEQLSALETRMNQAGFLDGRDMAT
TFNMMRANDLIWSFVVNNYLLGKDPFPFDLLYWNSDSTRMPAAMHSFYLRKMYQENRLVEPGAIELNGVKIDLGNIKTPL
YMLSTREDHIAPWQSTYALTQNVSGPIKFVLSASGHIAGVVNPPAANKYCYWTNAKKPKNPEVWLAGATQVEGSWWTDWQ
KWVEKFAGKQVPARQPGDGKLKAIEAAPGSYVQVKAV

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 66874; Mature: 66743

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQTGTESKMIDATELSKAMTSIAERSQRIVADFLTRQAADPTAAAADPLNIGNAFMEM
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
TAKLMSDPAKLVEANLNLWQDYMSLWQNTARRMLGEETQPVVAPDSGDRRFKDEMWQENE
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHCCCHH
IFDFIKQSYLLSARWMQGVVKNVEGLDDHTAKKVDFYTRQFVDAMAPSNFVLTNPEVLRA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHH
TVETRGENLLKGLNNLLDDLERGKGNLAIKMTDYDAFKVGENIAVTPGKVVFQTDLMQLI
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHCCCCEEECCCCEEHHHHHHHHH
QYDPTTPQAFEKPLLILPPWINKFYILDLRPKNSLIKWAVDQGHTVFVISWVNPDEKLAH
HCCCCCHHHHCCCEEEECCCCCEEEEEEECCCCCEEEEEECCCCEEEEEEECCCCHHHHH
KGFGDYMLEGPLAAIDAIEKATGSRQVNAAGYCLGGTLLACTLAYLAAKGEDRIASATFF
CCCCCHHHCCCHHHHHHHHHHCCCCEECCCCEEHHHHHHHHHHHHHHCCCCCCEEHHEEE
TTMTDFKDPGELGVFIDEEQLSALETRMNQAGFLDGRDMATTFNMMRANDLIWSFVVNNY
EEHHCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCE
LLGKDPFPFDLLYWNSDSTRMPAAMHSFYLRKMYQENRLVEPGAIELNGVKIDLGNIKTP
EECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCC
LYMLSTREDHIAPWQSTYALTQNVSGPIKFVLSASGHIAGVVNPPAANKYCYWTNAKKPK
EEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCCCEEEECCCCCCC
NPEVWLAGATQVEGSWWTDWQKWVEKFAGKQVPARQPGDGKLKAIEAAPGSYVQVKAV
CCCEEEEECEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEEEEC
>Mature Secondary Structure 
AEQTGTESKMIDATELSKAMTSIAERSQRIVADFLTRQAADPTAAAADPLNIGNAFMEM
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
TAKLMSDPAKLVEANLNLWQDYMSLWQNTARRMLGEETQPVVAPDSGDRRFKDEMWQENE
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHCCCHH
IFDFIKQSYLLSARWMQGVVKNVEGLDDHTAKKVDFYTRQFVDAMAPSNFVLTNPEVLRA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHH
TVETRGENLLKGLNNLLDDLERGKGNLAIKMTDYDAFKVGENIAVTPGKVVFQTDLMQLI
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHCCCCEEECCCCEEHHHHHHHHH
QYDPTTPQAFEKPLLILPPWINKFYILDLRPKNSLIKWAVDQGHTVFVISWVNPDEKLAH
HCCCCCHHHHCCCEEEECCCCCEEEEEEECCCCCEEEEEECCCCEEEEEEECCCCHHHHH
KGFGDYMLEGPLAAIDAIEKATGSRQVNAAGYCLGGTLLACTLAYLAAKGEDRIASATFF
CCCCCHHHCCCHHHHHHHHHHCCCCEECCCCEEHHHHHHHHHHHHHHCCCCCCEEHHEEE
TTMTDFKDPGELGVFIDEEQLSALETRMNQAGFLDGRDMATTFNMMRANDLIWSFVVNNY
EEHHCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCE
LLGKDPFPFDLLYWNSDSTRMPAAMHSFYLRKMYQENRLVEPGAIELNGVKIDLGNIKTP
EECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCC
LYMLSTREDHIAPWQSTYALTQNVSGPIKFVLSASGHIAGVVNPPAANKYCYWTNAKKPK
EEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCCCEEEECCCCCCC
NPEVWLAGATQVEGSWWTDWQKWVEKFAGKQVPARQPGDGKLKAIEAAPGSYVQVKAV
CCCEEEEECEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748438 [H]