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The map label for this gene is purU [H]

Identifier: 83312671

GI number: 83312671

Start: 3926091

End: 3926951

Strand: Reverse

Name: purU [H]

Synonym: amb3572

Alternate gene names: 83312671

Gene position: 3926951-3926091 (Counterclockwise)

Preceding gene: 83312673

Following gene: 83312669

Centisome position: 79.06

GC content: 64.34

Gene sequence:

>861_bases
ATGGCCGACAAGCCCGCCTGGGTGCTGACCATCACTTGTCCCGATACTGTCGGGATCGTGGCCGCCGTATCGGGCTTTCT
CAGCACCCATGACTGCTTCATTACCGAGGCGGCCCAGTTCGGCGATCCGCTGTCGTCGCGCTTCTTCATGCGCATCGTAT
TCGGGGCGGGCGCCATGACGCCGCCCATGGCCGAGCTGGAAAAGCTGTTCACCGGGGTGGCCGCCCAGTTTCAGATGATC
TGGAAGCTGCACGACGGCCGGCAGAAGGCCCGGGTGGTGATTCTGGTCTCCAAGTTCGGGCACTGCCTGAACGACCTGCT
GCACCGCTATCACACCGGTTCGCTGCCCATCGAGATTCCGGCGGTGATCTCCAACCACCAGGAGATGCGCTCCATCGTCG
AGTGGCACGGCATCCCCTATCACTACCTGGCGGTGGACAAGCACGACAAGCTGACCCAGGAAAACCGGGTGATGGAGGTG
ATCGAACGCGCCGACGCCGACCTGGTGGTGCTGGCCCGCTACATGCAAATCCTGTCGACCGACATGTGCGTGCGGCTGCA
GGGCAAGGCCATCAACATCCACCACTCGTTCCTGCCCAGCTTCAAGGGCGCCAAGCCCTATCACCAGGCCCATTCCCGCG
GCGTGAAGATCATCGGCGCCACCGCCCATTACGTCACCCCCGACCTGGACGAGGGTCCCATCATCGAGCAGGGCGTCGAG
CGCGTCGACCACACCCACACCCCCGACGATCTGGTGGCCATCGGCCGCGACATCGAGAACGTGGTGCTGGCCCGCGCCGT
GCGCTGGCACACCGAGCATCGGGTGCTGCTCAACGGCTCGAAGACGGTGGTGTTCCGCTGA

Upstream 100 bases:

>100_bases
CCGGACCTTCGGGCATTTGCGGCCAAAAGGCAAGGGCGGGATTGCCCATTGGGGCGGGGTCGGATAAACCCATGGGGTCA
TTGCAACAAGGAGCCTGATC

Downstream 100 bases:

>100_bases
CGCCAGCGCCCGGCGCGAGTCGTGACGATCCGTCGGCAAGGCCGACGGGGCCGTGCGCTGGCACACCGAGCATCGGGTGC
TGCTCAACGGCTCGAAGACG

Product: formyltetrahydrofolate hydrolase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MADKPAWVLTITCPDTVGIVAAVSGFLSTHDCFITEAAQFGDPLSSRFFMRIVFGAGAMTPPMAELEKLFTGVAAQFQMI
WKLHDGRQKARVVILVSKFGHCLNDLLHRYHTGSLPIEIPAVISNHQEMRSIVEWHGIPYHYLAVDKHDKLTQENRVMEV
IERADADLVVLARYMQILSTDMCVRLQGKAINIHHSFLPSFKGAKPYHQAHSRGVKIIGATAHYVTPDLDEGPIIEQGVE
RVDHTHTPDDLVAIGRDIENVVLARAVRWHTEHRVLLNGSKTVVFR

Sequences:

>Translated_286_residues
MADKPAWVLTITCPDTVGIVAAVSGFLSTHDCFITEAAQFGDPLSSRFFMRIVFGAGAMTPPMAELEKLFTGVAAQFQMI
WKLHDGRQKARVVILVSKFGHCLNDLLHRYHTGSLPIEIPAVISNHQEMRSIVEWHGIPYHYLAVDKHDKLTQENRVMEV
IERADADLVVLARYMQILSTDMCVRLQGKAINIHHSFLPSFKGAKPYHQAHSRGVKIIGATAHYVTPDLDEGPIIEQGVE
RVDHTHTPDDLVAIGRDIENVVLARAVRWHTEHRVLLNGSKTVVFR
>Mature_285_residues
ADKPAWVLTITCPDTVGIVAAVSGFLSTHDCFITEAAQFGDPLSSRFFMRIVFGAGAMTPPMAELEKLFTGVAAQFQMIW
KLHDGRQKARVVILVSKFGHCLNDLLHRYHTGSLPIEIPAVISNHQEMRSIVEWHGIPYHYLAVDKHDKLTQENRVMEVI
ERADADLVVLARYMQILSTDMCVRLQGKAINIHHSFLPSFKGAKPYHQAHSRGVKIIGATAHYVTPDLDEGPIIEQGVER
VDHTHTPDDLVAIGRDIENVVLARAVRWHTEHRVLLNGSKTVVFR

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=174, Percent_Identity=33.9080459770115, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI209869995, Length=174, Percent_Identity=33.9080459770115, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI209869993, Length=174, Percent_Identity=33.9080459770115, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI1787483, Length=285, Percent_Identity=41.0526315789474, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1788846, Length=188, Percent_Identity=28.7234042553192, Blast_Score=75, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17567511, Length=163, Percent_Identity=34.3558282208589, Blast_Score=88, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32064; Mature: 31933

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKPAWVLTITCPDTVGIVAAVSGFLSTHDCFITEAAQFGDPLSSRFFMRIVFGAGAMT
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
PPMAELEKLFTGVAAQFQMIWKLHDGRQKARVVILVSKFGHCLNDLLHRYHTGSLPIEIP
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCEEEH
AVISNHQEMRSIVEWHGIPYHYLAVDKHDKLTQENRVMEVIERADADLVVLARYMQILST
HHHCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
DMCVRLQGKAINIHHSFLPSFKGAKPYHQAHSRGVKIIGATAHYVTPDLDEGPIIEQGVE
CCEEEEECCEEEEEHHHCCCCCCCCCHHHHHHCCEEEEEEEEHEECCCCCCCCCHHHHHH
RVDHTHTPDDLVAIGRDIENVVLARAVRWHTEHRVLLNGSKTVVFR
HHCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCEEEEECCEEEEEC
>Mature Secondary Structure 
ADKPAWVLTITCPDTVGIVAAVSGFLSTHDCFITEAAQFGDPLSSRFFMRIVFGAGAMT
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
PPMAELEKLFTGVAAQFQMIWKLHDGRQKARVVILVSKFGHCLNDLLHRYHTGSLPIEIP
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCEEEH
AVISNHQEMRSIVEWHGIPYHYLAVDKHDKLTQENRVMEVIERADADLVVLARYMQILST
HHHCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
DMCVRLQGKAINIHHSFLPSFKGAKPYHQAHSRGVKIIGATAHYVTPDLDEGPIIEQGVE
CCEEEEECCEEEEEHHHCCCCCCCCCHHHHHHCCEEEEEEEEHEECCCCCCCCCHHHHHH
RVDHTHTPDDLVAIGRDIENVVLARAVRWHTEHRVLLNGSKTVVFR
HHCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCEEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]