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The map label for this gene is nasR [H]

Identifier: 83312482

GI number: 83312482

Start: 3673964

End: 3676099

Strand: Reverse

Name: nasR [H]

Synonym: amb3383

Alternate gene names: 83312482

Gene position: 3676099-3673964 (Counterclockwise)

Preceding gene: 83312483

Following gene: 83312480

Centisome position: 74.01

GC content: 67.13

Gene sequence:

>2136_bases
ATGACCTTTGTATGGTGTTGGCGGGTCCTGGATGGGGAGCTTCGGCTCCTGTCCAGAGAGTTCAGGTTCGGAGGGTTGCC
CATGTTCCGGCTGACCGATCTCAGGATTTCAGCAAAGATTGCCCTGTCCTTCTTGTTCCCGGTCATTCTGATTCTTGGGC
TGGCGGGCTATGTGATCAGTGACAAGGCGCGGGTGGTGTCGGAGACGTCGACGCTGGCCGGGATCGCTCCGCTGACCGCC
GAGATCAGCGCCCTGGTGCACGAAGTGCAGAAGGAACGCGGCGCCTCGGCCGTCTTCATCGGCAGCAAGGGCGAGAAGTT
TGGCGCGGAACTCAAGGCCCAGCGCCAGCTGACCGACGCGGCGCGGGAAAAGCTGACCCGGTCCCTGGCCGGGATTGACG
TCTCGGCCCTGGGTGCCAGCTTTCCACCCAAGGTGGACAAGGCGACAAAGCGTCTGGAAGCGCTGATCGCCAGCCGGGGC
GCCATCGATGCCCTGGCCATGGACGGCCGTACCGCCCTGGGCAACTTCACCGAAACCATTCGGTTTCAGTTGGATATGGT
CAACCAGATCGCCGTACTGTCCAGTGACGCCAGGGTGGGGCGCATGATCGGCGCCTATCTCAAGCTGATGGAGGGCAAGG
AAAAGGCCGGGCAGGAGCGGGCCACCGCTGCCGGCGCCTTCTCCTCGGGCAAGTTCGAGCCGGAGGTCTATCGCCGCTTC
GTCGCCATCGTCGCCGAGCAGCAGCTGTTTCTGGGGGAGTTTCAGGAAAGCGCCGCCCCCGCCCAGGTGGATTTCTTCCG
TACCACCCTGGATGACGAGGCAAGCCGCACGGTGGAGCGCATGCGCAAGGTCGGTCTGGACAGCCCCGCCACCGGCACGG
TCGGCGATGTCACCGGCCCGGCCTGGTTCGCCGCCGCCACCTCGCGCATCAATCTTTTGAAGAAGGTCGAGGACCACATG
GCCGCCGATGTCCAGGCCCTGGCCGCCCAGGTCAACGGCGATGCCAGGGGCGTGCTGCTCACCACCGTCCTGGGCGTCGT
CGCCGCCCTGGTGATTGCCCTGGCCGTGGCCTGGACCGTGGTGCGCCAGCTGACCTCGGCCATCGGTTCCCTGGTCGGCA
CCATGGAACGCCTGGCCGCTGACGACTTCAGCGTGAGCGTCGGGGGAACGGAGCGGGCCGACGAGATGGGGGCCATGGCC
CGCTCGGTCCAGATCTTCAAGGACGCCATGATGCGGGGACATGATCTGGCCAGGAAGCAACTGGCCGAGGCTCATAACGG
CGAATTGCGCGGCCGGGCCATCGAGGCGCTGGTCAACCGTTTCCAGGGTTCGGCCGAGCGCATGGTCGCGGCGGTCTCGT
CGGCGGCCCAGCGGTTGCAGGGCACCGCCGCCGCCATGAGCGCGGCCGCCAGTGATACCAATGATCGGGCCACGGTGGTG
GCGGGCGCCACCGAACTGGCGTCGGCCAATGTCCAGACCGTGGCCGCCGCCGCCGAGGAACTGACCTCCTCCATCCAGGA
GATCGGCCGTCAGGTTCACCGCTCGGCGGAGATTTCCGGCAATGCGGTGGCCGAAGCCGGCGACGCCCAGAATGACGTCA
CCGGCCTCGCCACCATGGTGGGCAAGATCGGCGAAGTGGTGACCCTGATCAACGACATCGCGGCCCAGACCAATCTTCTG
GCGCTCAACGCCACCATCGAGGCGGCGCGGGCCGGCGAGGCGGGCAAGGGATTTGCCGTGGTGGCGGGCGAGGTGAAGAA
CCTCGCCAATCAGACCGCCCGGGCCACCGGCGAGATCGGCCAGCAGATCTCTTCGGTGCAGCAGCAGACCGAAAAGGTGG
TGGGCGCCATCTCCGATATCGTCACCATCATCCGCGAGGTGGGCGAGATCACCGCCAGCATCGCCAGCGCGGTGGAGGAG
CAGAGCGCCGCCACCCAGGAGATCGCCCGCAGCGTCGAGCAGGCGGCCGCCGGCACCGCCGAGGTCTCGGCCAATGTGGG
CGGAGTCCAGGACGCGGCGGGCCGCACCGGCGGCGCCGCCGTCGAGGTGCTGGATGCGTCCAGGGCCATGGGCGAGGAGG
CGGTGAACCTCAAGACCACTATCGACGGTTTCCTGGGCGAAATCCGCGCGGTGTAG

Upstream 100 bases:

>100_bases
GAGGGCGAGCGGGCGGTCTGATTCCGTGCGCAAATTCCTGCCGTGACACTTCCCTGCTTGAAAATATGCTCGAGCAATAC
AACATTGAGCATGTTTAGCC

Downstream 100 bases:

>100_bases
GGGGAAGAGGCACTACCGCGCCTTGTCCAGGGCGGCCAGCACCTCGGCCTCGCTCAGCAGTTCCGGCAGGGCGATGCCGT
CCGGTGCGCTGGGGCCATAG

Product: methyl-accepting chemotaxis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 711; Mature: 710

Protein sequence:

>711_residues
MTFVWCWRVLDGELRLLSREFRFGGLPMFRLTDLRISAKIALSFLFPVILILGLAGYVISDKARVVSETSTLAGIAPLTA
EISALVHEVQKERGASAVFIGSKGEKFGAELKAQRQLTDAAREKLTRSLAGIDVSALGASFPPKVDKATKRLEALIASRG
AIDALAMDGRTALGNFTETIRFQLDMVNQIAVLSSDARVGRMIGAYLKLMEGKEKAGQERATAAGAFSSGKFEPEVYRRF
VAIVAEQQLFLGEFQESAAPAQVDFFRTTLDDEASRTVERMRKVGLDSPATGTVGDVTGPAWFAAATSRINLLKKVEDHM
AADVQALAAQVNGDARGVLLTTVLGVVAALVIALAVAWTVVRQLTSAIGSLVGTMERLAADDFSVSVGGTERADEMGAMA
RSVQIFKDAMMRGHDLARKQLAEAHNGELRGRAIEALVNRFQGSAERMVAAVSSAAQRLQGTAAAMSAAASDTNDRATVV
AGATELASANVQTVAAAAEELTSSIQEIGRQVHRSAEISGNAVAEAGDAQNDVTGLATMVGKIGEVVTLINDIAAQTNLL
ALNATIEAARAGEAGKGFAVVAGEVKNLANQTARATGEIGQQISSVQQQTEKVVGAISDIVTIIREVGEITASIASAVEE
QSAATQEIARSVEQAAAGTAEVSANVGGVQDAAGRTGGAAVEVLDASRAMGEEAVNLKTTIDGFLGEIRAV

Sequences:

>Translated_711_residues
MTFVWCWRVLDGELRLLSREFRFGGLPMFRLTDLRISAKIALSFLFPVILILGLAGYVISDKARVVSETSTLAGIAPLTA
EISALVHEVQKERGASAVFIGSKGEKFGAELKAQRQLTDAAREKLTRSLAGIDVSALGASFPPKVDKATKRLEALIASRG
AIDALAMDGRTALGNFTETIRFQLDMVNQIAVLSSDARVGRMIGAYLKLMEGKEKAGQERATAAGAFSSGKFEPEVYRRF
VAIVAEQQLFLGEFQESAAPAQVDFFRTTLDDEASRTVERMRKVGLDSPATGTVGDVTGPAWFAAATSRINLLKKVEDHM
AADVQALAAQVNGDARGVLLTTVLGVVAALVIALAVAWTVVRQLTSAIGSLVGTMERLAADDFSVSVGGTERADEMGAMA
RSVQIFKDAMMRGHDLARKQLAEAHNGELRGRAIEALVNRFQGSAERMVAAVSSAAQRLQGTAAAMSAAASDTNDRATVV
AGATELASANVQTVAAAAEELTSSIQEIGRQVHRSAEISGNAVAEAGDAQNDVTGLATMVGKIGEVVTLINDIAAQTNLL
ALNATIEAARAGEAGKGFAVVAGEVKNLANQTARATGEIGQQISSVQQQTEKVVGAISDIVTIIREVGEITASIASAVEE
QSAATQEIARSVEQAAAGTAEVSANVGGVQDAAGRTGGAAVEVLDASRAMGEEAVNLKTTIDGFLGEIRAV
>Mature_710_residues
TFVWCWRVLDGELRLLSREFRFGGLPMFRLTDLRISAKIALSFLFPVILILGLAGYVISDKARVVSETSTLAGIAPLTAE
ISALVHEVQKERGASAVFIGSKGEKFGAELKAQRQLTDAAREKLTRSLAGIDVSALGASFPPKVDKATKRLEALIASRGA
IDALAMDGRTALGNFTETIRFQLDMVNQIAVLSSDARVGRMIGAYLKLMEGKEKAGQERATAAGAFSSGKFEPEVYRRFV
AIVAEQQLFLGEFQESAAPAQVDFFRTTLDDEASRTVERMRKVGLDSPATGTVGDVTGPAWFAAATSRINLLKKVEDHMA
ADVQALAAQVNGDARGVLLTTVLGVVAALVIALAVAWTVVRQLTSAIGSLVGTMERLAADDFSVSVGGTERADEMGAMAR
SVQIFKDAMMRGHDLARKQLAEAHNGELRGRAIEALVNRFQGSAERMVAAVSSAAQRLQGTAAAMSAAASDTNDRATVVA
GATELASANVQTVAAAAEELTSSIQEIGRQVHRSAEISGNAVAEAGDAQNDVTGLATMVGKIGEVVTLINDIAAQTNLLA
LNATIEAARAGEAGKGFAVVAGEVKNLANQTARATGEIGQQISSVQQQTEKVVGAISDIVTIIREVGEITASIASAVEEQ
SAATQEIARSVEQAAAGTAEVSANVGGVQDAAGRTGGAAVEVLDASRAMGEEAVNLKTTIDGFLGEIRAV

Specific function: Nitrate- and nitrite-responsive positive regulator for nasFEDCBA operon expression. NasR protein binds to the factor- independent terminator site located in the nasF operon leader RNA to effect transcription antitermination [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 NIT (nitrate and nitrite sensing) domain [H]

Homologues:

Organism=Escherichia coli, GI1788194, Length=198, Percent_Identity=39.8989898989899, Blast_Score=112, Evalue=1e-25,
Organism=Escherichia coli, GI1787690, Length=274, Percent_Identity=30.6569343065693, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1789453, Length=363, Percent_Identity=28.099173553719, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI2367378, Length=313, Percent_Identity=31.9488817891374, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1788195, Length=196, Percent_Identity=35.7142857142857, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005561
- InterPro:   IPR011006
- InterPro:   IPR013587
- InterPro:   IPR010910
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03861 ANTAR; PF08376 NIT [H]

EC number: NA

Molecular weight: Translated: 74327; Mature: 74196

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50885 HAMP ; PS50906 NIT ; PS00687 ALDEHYDE_DEHYDR_GLU ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFVWCWRVLDGELRLLSREFRFGGLPMFRLTDLRISAKIALSFLFPVILILGLAGYVIS
CCEEEEEHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHC
DKARVVSETSTLAGIAPLTAEISALVHEVQKERGASAVFIGSKGEKFGAELKAQRQLTDA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHH
AREKLTRSLAGIDVSALGASFPPKVDKATKRLEALIASRGAIDALAMDGRTALGNFTETI
HHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCHHHCCHHHHH
RFQLDMVNQIAVLSSDARVGRMIGAYLKLMEGKEKAGQERATAAGAFSSGKFEPEVYRRF
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHCHHHHHHHCCCCCCCCCHHHHHHH
VAIVAEQQLFLGEFQESAAPAQVDFFRTTLDDEASRTVERMRKVGLDSPATGTVGDVTGP
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
AWFAAATSRINLLKKVEDHMAADVQALAAQVNGDARGVLLTTVLGVVAALVIALAVAWTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VRQLTSAIGSLVGTMERLAADDFSVSVGGTERADEMGAMARSVQIFKDAMMRGHDLARKQ
HHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAEAHNGELRGRAIEALVNRFQGSAERMVAAVSSAAQRLQGTAAAMSAAASDTNDRATVV
HHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
AGATELASANVQTVAAAAEELTSSIQEIGRQVHRSAEISGNAVAEAGDAQNDVTGLATMV
ECHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHH
GKIGEVVTLINDIAAQTNLLALNATIEAARAGEAGKGFAVVAGEVKNLANQTARATGEIG
HHHHHHHHHHHHHHHHHCEEEEECHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHH
QQISSVQQQTEKVVGAISDIVTIIREVGEITASIASAVEEQSAATQEIARSVEQAAAGTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
EVSANVGGVQDAAGRTGGAAVEVLDASRAMGEEAVNLKTTIDGFLGEIRAV
HHCCCCCCCCHHCCCCCCCHHHHHHHHHHHCHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TFVWCWRVLDGELRLLSREFRFGGLPMFRLTDLRISAKIALSFLFPVILILGLAGYVIS
CEEEEEHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHC
DKARVVSETSTLAGIAPLTAEISALVHEVQKERGASAVFIGSKGEKFGAELKAQRQLTDA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHH
AREKLTRSLAGIDVSALGASFPPKVDKATKRLEALIASRGAIDALAMDGRTALGNFTETI
HHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCHHHCCHHHHH
RFQLDMVNQIAVLSSDARVGRMIGAYLKLMEGKEKAGQERATAAGAFSSGKFEPEVYRRF
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHCHHHHHHHCCCCCCCCCHHHHHHH
VAIVAEQQLFLGEFQESAAPAQVDFFRTTLDDEASRTVERMRKVGLDSPATGTVGDVTGP
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
AWFAAATSRINLLKKVEDHMAADVQALAAQVNGDARGVLLTTVLGVVAALVIALAVAWTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VRQLTSAIGSLVGTMERLAADDFSVSVGGTERADEMGAMARSVQIFKDAMMRGHDLARKQ
HHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAEAHNGELRGRAIEALVNRFQGSAERMVAAVSSAAQRLQGTAAAMSAAASDTNDRATVV
HHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
AGATELASANVQTVAAAAEELTSSIQEIGRQVHRSAEISGNAVAEAGDAQNDVTGLATMV
ECHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHH
GKIGEVVTLINDIAAQTNLLALNATIEAARAGEAGKGFAVVAGEVKNLANQTARATGEIG
HHHHHHHHHHHHHHHHHCEEEEECHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHH
QQISSVQQQTEKVVGAISDIVTIIREVGEITASIASAVEEQSAATQEIARSVEQAAAGTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
EVSANVGGVQDAAGRTGGAAVEVLDASRAMGEEAVNLKTTIDGFLGEIRAV
HHCCCCCCCCHHCCCCCCCHHHHHHHHHHHCHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8051020; 9769209 [H]