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The map label for this gene is tnpR [H]

Identifier: 83312408

GI number: 83312408

Start: 3590930

End: 3591538

Strand: Reverse

Name: tnpR [H]

Synonym: amb3309

Alternate gene names: 83312408

Gene position: 3591538-3590930 (Counterclockwise)

Preceding gene: 83312410

Following gene: 83312406

Centisome position: 72.31

GC content: 62.4

Gene sequence:

>609_bases
ATGAGTATTGAGAAGGAGGGGGGGATGTTTGTCGGTTACGCCAGAGTTTCGACCCAGGACCAGTCGCCGCAACTGCAACT
CGACGCCCTGCATGCGGCCGGGTGTGAACGCATTTTCAGTGAACATGCCTCCGGCGCCCGCGCCGATCGCCCCGAGTTGA
AGGCCGCCTTGGACTTCGCTCGCAACGGCGACACGGTGGTGGTGTGGAAGCTGGATCGGTTGGCTCGATCGACCCGGCAG
TTGATCGAGACTGTCGATATGCTCGACGGGCGTGGGATCGGTTTACGGTCGTTGACCGAAGCGATCGACACCACCACCGC
CGGGGGGAAATTGATCTTCCACGTTTTTGCGGCGCTCGGGGAGTTCGAGAGAAGTTTGATCATCGAGCGGACTCGAGCCG
GACTGGATTCGGCCCGCAAAATGGGCCGGGTCGGCGGTCGGCCTCGGTCCCTGTCTGGTCAGGATGTGGAGGTGGCGCGA
GCCATGCTTGCATCCCCGGCGATTACTGTTGAGGAGGTGGCCCGGCGCCTCAATGTTTCGACTGCCACTTTGTATCGCCA
CCTGCCAGGTGGGCGTGGTGGTGGCCTGGATCTTCGACCTGCGGGCTGA

Upstream 100 bases:

>100_bases
TGAGAATTTATTTGTGTCGTGGTCTTTTGCGTGATTAGCGGAGGTGGTATTATCTCAAAACTGTCTCTCAAAACATGCAT
CTCAATTCGTCGGTAATTGA

Downstream 100 bases:

>100_bases
TGTGGTGGTGGCTGTGCTCGCGGATTAGGCTGGTGCCCAGGGAGCCGGTTACGGCTCTACTAAGTAAACGCCCAATCCAT
TTACTTAGTGATTACTAAGT

Product: site-specific recombinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MSIEKEGGMFVGYARVSTQDQSPQLQLDALHAAGCERIFSEHASGARADRPELKAALDFARNGDTVVVWKLDRLARSTRQ
LIETVDMLDGRGIGLRSLTEAIDTTTAGGKLIFHVFAALGEFERSLIIERTRAGLDSARKMGRVGGRPRSLSGQDVEVAR
AMLASPAITVEEVARRLNVSTATLYRHLPGGRGGGLDLRPAG

Sequences:

>Translated_202_residues
MSIEKEGGMFVGYARVSTQDQSPQLQLDALHAAGCERIFSEHASGARADRPELKAALDFARNGDTVVVWKLDRLARSTRQ
LIETVDMLDGRGIGLRSLTEAIDTTTAGGKLIFHVFAALGEFERSLIIERTRAGLDSARKMGRVGGRPRSLSGQDVEVAR
AMLASPAITVEEVARRLNVSTATLYRHLPGGRGGGLDLRPAG
>Mature_201_residues
SIEKEGGMFVGYARVSTQDQSPQLQLDALHAAGCERIFSEHASGARADRPELKAALDFARNGDTVVVWKLDRLARSTRQL
IETVDMLDGRGIGLRSLTEAIDTTTAGGKLIFHVFAALGEFERSLIIERTRAGLDSARKMGRVGGRPRSLSGQDVEVARA
MLASPAITVEEVARRLNVSTATLYRHLPGGRGGGLDLRPAG

Specific function: Resolvase catalyzes the resolution (a site-specific recombination) of the cointegrated replicon to yield the final transposition products [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=183, Percent_Identity=48.6338797814208, Blast_Score=170, Evalue=6e-44,
Organism=Escherichia coli, GI1787638, Length=182, Percent_Identity=34.0659340659341, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1787827, Length=182, Percent_Identity=33.5164835164835, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 21754; Mature: 21623

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEKEGGMFVGYARVSTQDQSPQLQLDALHAAGCERIFSEHASGARADRPELKAALDFA
CCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
RNGDTVVVWKLDRLARSTRQLIETVDMLDGRGIGLRSLTEAIDTTTAGGKLIFHVFAALG
CCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
EFERSLIIERTRAGLDSARKMGRVGGRPRSLSGQDVEVARAMLASPAITVEEVARRLNVS
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCH
TATLYRHLPGGRGGGLDLRPAG
HHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SIEKEGGMFVGYARVSTQDQSPQLQLDALHAAGCERIFSEHASGARADRPELKAALDFA
CCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
RNGDTVVVWKLDRLARSTRQLIETVDMLDGRGIGLRSLTEAIDTTTAGGKLIFHVFAALG
CCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
EFERSLIIERTRAGLDSARKMGRVGGRPRSLSGQDVEVARAMLASPAITVEEVARRLNVS
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCH
TATLYRHLPGGRGGGLDLRPAG
HHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2170815; 2555186 [H]