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The map label for this gene is pinE [C]

Identifier: 83312369

GI number: 83312369

Start: 3535622

End: 3536050

Strand: Reverse

Name: pinE [C]

Synonym: amb3270

Alternate gene names: 83312369

Gene position: 3536050-3535622 (Counterclockwise)

Preceding gene: 83312370

Following gene: 83312367

Centisome position: 71.19

GC content: 62.24

Gene sequence:

>429_bases
GTGGATGTTGCTGCTCGCGACCAGCTTGCTGCCGCTCTGGAGTTTGTCCGAGAGGGCGATGTCCTGATGGTGAGCAAGTT
GGATCGGCTGGCCCGATCCGTCCCTCATCTCCTCCAGATCACCGAATATCTGGAACAGAAGAAGGTGGCCCTGCGGGTTC
TCAACATGGCGATGGACACAAGCACGCCGACGGGACGGCTTATGCTTACCTTGCTTGGCGGGATCGGTGCGTGGGAACGA
GAAATCATGTTGGAGCGCCAGCGGGAGGGTATCGCCAAGGCCAAGGCCGAAGGCAAATACAAGGGCCGCCGCCCGACGAT
AAAGCCCGAGGAAATCCGTGCCCTGAAGGACGAGGGGCTTGGGGCGGCTGCCATCGCCCGCAAGCTGGGGGTCGGGCGGG
CCAGCGTTTACCGGTTGCTGGCCGAATAG

Upstream 100 bases:

>100_bases
GAGAACATCCACTCTTGATCAGCGGGCCGGCCTTGATGCCCAGTACCGAGACCTGGAAGCTGCCGGTTGCGAAAAAATCT
TCACGGAGCAAGTTTCGTCC

Downstream 100 bases:

>100_bases
CCATTTCGGCTACCGGCCCGTTGCCATATTTCTCCCCATACTCTGGCTTCTAAAACCTTTAACCCATTGATTCCATGCTC
GTATTCGCCTCGATCTTCAT

Product: site-specific recombinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 142; Mature: 142

Protein sequence:

>142_residues
MDVAARDQLAAALEFVREGDVLMVSKLDRLARSVPHLLQITEYLEQKKVALRVLNMAMDTSTPTGRLMLTLLGGIGAWER
EIMLERQREGIAKAKAEGKYKGRRPTIKPEEIRALKDEGLGAAAIARKLGVGRASVYRLLAE

Sequences:

>Translated_142_residues
MDVAARDQLAAALEFVREGDVLMVSKLDRLARSVPHLLQITEYLEQKKVALRVLNMAMDTSTPTGRLMLTLLGGIGAWER
EIMLERQREGIAKAKAEGKYKGRRPTIKPEEIRALKDEGLGAAAIARKLGVGRASVYRLLAE
>Mature_142_residues
MDVAARDQLAAALEFVREGDVLMVSKLDRLARSVPHLLQITEYLEQKKVALRVLNMAMDTSTPTGRLMLTLLGGIGAWER
EIMLERQREGIAKAKAEGKYKGRRPTIKPEEIRALKDEGLGAAAIARKLGVGRASVYRLLAE

Specific function: Site-specific recombination protein [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=108, Percent_Identity=39.8148148148148, Blast_Score=88, Evalue=2e-19,
Organism=Escherichia coli, GI1787827, Length=124, Percent_Identity=31.4516129032258, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1787638, Length=124, Percent_Identity=30.6451612903226, Blast_Score=73, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 15712; Mature: 15712

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVAARDQLAAALEFVREGDVLMVSKLDRLARSVPHLLQITEYLEQKKVALRVLNMAMDT
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
STPTGRLMLTLLGGIGAWEREIMLERQREGIAKAKAEGKYKGRRPTIKPEEIRALKDEGL
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCC
GAAAIARKLGVGRASVYRLLAE
CHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MDVAARDQLAAALEFVREGDVLMVSKLDRLARSVPHLLQITEYLEQKKVALRVLNMAMDT
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
STPTGRLMLTLLGGIGAWEREIMLERQREGIAKAKAEGKYKGRRPTIKPEEIRALKDEGL
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCC
GAAAIARKLGVGRASVYRLLAE
CHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1331988 [H]