Click here to switch to the map view.

The map label for this gene is amiC [H]

Identifier: 83312337

GI number: 83312337

Start: 3500424

End: 3502118

Strand: Reverse

Name: amiC [H]

Synonym: amb3238

Alternate gene names: 83312337

Gene position: 3502118-3500424 (Counterclockwise)

Preceding gene: 83312338

Following gene: 83312336

Centisome position: 70.51

GC content: 70.68

Gene sequence:

>1695_bases
ATGTTCCCTTGGCGACCCGTGACCGTGAGGCAGATGAGGACAAGAGCGCGTTTGTGGCCCGTGCTGGCGGCCCTGGTTCT
GACCCTGACCTCGTTCGGGCTGGGCGGGGTGACCGCGTCCGAGGCGGCGACCGCGTCGGGGGCCAGGTTGGGCATCCACG
GCGAAGGGGTCACCCGGTTCGTCCTGGACCTGTCCGATCAGGTGGCCTTCAAGATCACGCCCCTGGCCGAGCCCTATCGC
ATCGCCATCGACCTGTCCGGCGCGGATTACAGCGGGCCGGGGGGCATCTCCAAGCCTTGGGGATCGGTCAATTCCATGCA
TCTTGACGGCGGCCGGATCGTTCTGGACCTGCGCAAGCCCGCTTTGGTCAAGAGCGCCTTCATCATCGCGCCGCGCGACG
GCATGGGGCACCGCCTGGTGGTGGATCTGGCCGAGACCACGCGCGAGGCATTCCTGGCGGCGGCCGGGTCCGGCCCGGCC
AGCGCCAGGGCGCCGGTGCTCAAGCCCCCGGCGGCGGCCAAGGCCGTCGCTCCGGTGCCCGCCGCCGCCAAGCCTTCCCC
GGCTCCCGTCCTGGTCCCCCCCAAGCCGGCCGAGGAATTCCATCTGCCCGAGGCTGCGCCCGCCGAGGCGGCGCGTCCCG
CCACCGCGCCCTCCGCCAGCCGCATCGTCGTCAACAGCCCCAATGCTCCGGCCGAGTCCATCGTGCCGCCGACCGAGCCC
GTCACCCTGGTTCCGCCGCCGCCGAGTCAGGTTCCGGTTCCGGTGGAGCGGCCGCAACTGGCCGAGAAGCCGGCGGTGGA
GAAGGGAGCCCGGGCACAGGCGGCAACGCCCATGCTGTCGGCGCCCATGCCGCCGCCGCCCGAACGGCCCCAGCCCGTCG
CGGCCCCCCAGCCCGTCGCCGCGCCCCAGCCTCCGGTGCCCCAGCCGCCCGCCCCCGTGCAGCAGCAGGCCGCGCCGGTG
CCGACCCCCTCCATGGAGAGCGTCGCCAAGGCCAAGGACGGCGTGCCGGTGATCGTCATCGACCCCGGTCACGGCGGCGT
CGACCCCGGCGCCACCGGCGTGTCGGGGACCTATGAAAAGCACATCACCCTGGCCATGGCCCGCGAACTGAAGGCCATGC
TGGAGCGCAACGGCCGCTATCGCGTGCATCTGACCCGTGACCGCGACGTGTTCATCCGCCTGCGCGAGCGCATCGCCATC
GCCCGGGCCCAGGGCGCCGACCTGTTCATCTCGCTGCACGCCGACGCGGTGCAAAGCCCGCAGATCAGGGGCCTGTCCGT
CTATACCTTGTCGCGCAACGCCTCGGACGCCGAGGCGCAGGCGCTGGCGGAAAAGGAAAACAAGGCCGACCTCATCGCCG
GCATCGATCTGACCCATGAATCCGCCGACGTGGCCAACATCCTCATCGACCTCGCCCAGCGCGAGACCATGAACCGCTCG
GCCGGGTTCGCCACCGAACTGGTGGACGAGGTGGGCCAGGAAATGGACCTGCTGGGCAATACCCACCGTTTCGCCGGCTT
TGCCGTGCTGAAGGCGCCCGACGTGCCGGCGGTCCTGGTGGAGATGGGCTATCTCTCCAATGAATCCGAGGAGAAGATGC
TGCGCCAGCCGCAGTACCGCGCCCGTCTGGCTAAGTCCATCGCCAAGGCGGTGGAGCGCTTCTTCCCGCCCAACCTCAAG
GCCAAGCGGCCCTAA

Upstream 100 bases:

>100_bases
GCACCCGGTCCGACCAGTGGACGAAATCAGGCGAAGAGTCTGCTGAATCAATGCGTTTTGGGTTGAGCTTGAGTCAGCGT
CTGCGGTATAGCTGTCACTG

Downstream 100 bases:

>100_bases
GTCATGCGGCTGACCGCCCTTGCCGTGCTGGCGCTGATGGCCTGGGCTCTTCCCGTCCGGGCCGAGACCAAGCCCACGGT
GCCGTGCCTGACCTGTCCCC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MFPWRPVTVRQMRTRARLWPVLAALVLTLTSFGLGGVTASEAATASGARLGIHGEGVTRFVLDLSDQVAFKITPLAEPYR
IAIDLSGADYSGPGGISKPWGSVNSMHLDGGRIVLDLRKPALVKSAFIIAPRDGMGHRLVVDLAETTREAFLAAAGSGPA
SARAPVLKPPAAAKAVAPVPAAAKPSPAPVLVPPKPAEEFHLPEAAPAEAARPATAPSASRIVVNSPNAPAESIVPPTEP
VTLVPPPPSQVPVPVERPQLAEKPAVEKGARAQAATPMLSAPMPPPPERPQPVAAPQPVAAPQPPVPQPPAPVQQQAAPV
PTPSMESVAKAKDGVPVIVIDPGHGGVDPGATGVSGTYEKHITLAMARELKAMLERNGRYRVHLTRDRDVFIRLRERIAI
ARAQGADLFISLHADAVQSPQIRGLSVYTLSRNASDAEAQALAEKENKADLIAGIDLTHESADVANILIDLAQRETMNRS
AGFATELVDEVGQEMDLLGNTHRFAGFAVLKAPDVPAVLVEMGYLSNESEEKMLRQPQYRARLAKSIAKAVERFFPPNLK
AKRP

Sequences:

>Translated_564_residues
MFPWRPVTVRQMRTRARLWPVLAALVLTLTSFGLGGVTASEAATASGARLGIHGEGVTRFVLDLSDQVAFKITPLAEPYR
IAIDLSGADYSGPGGISKPWGSVNSMHLDGGRIVLDLRKPALVKSAFIIAPRDGMGHRLVVDLAETTREAFLAAAGSGPA
SARAPVLKPPAAAKAVAPVPAAAKPSPAPVLVPPKPAEEFHLPEAAPAEAARPATAPSASRIVVNSPNAPAESIVPPTEP
VTLVPPPPSQVPVPVERPQLAEKPAVEKGARAQAATPMLSAPMPPPPERPQPVAAPQPVAAPQPPVPQPPAPVQQQAAPV
PTPSMESVAKAKDGVPVIVIDPGHGGVDPGATGVSGTYEKHITLAMARELKAMLERNGRYRVHLTRDRDVFIRLRERIAI
ARAQGADLFISLHADAVQSPQIRGLSVYTLSRNASDAEAQALAEKENKADLIAGIDLTHESADVANILIDLAQRETMNRS
AGFATELVDEVGQEMDLLGNTHRFAGFAVLKAPDVPAVLVEMGYLSNESEEKMLRQPQYRARLAKSIAKAVERFFPPNLK
AKRP
>Mature_564_residues
MFPWRPVTVRQMRTRARLWPVLAALVLTLTSFGLGGVTASEAATASGARLGIHGEGVTRFVLDLSDQVAFKITPLAEPYR
IAIDLSGADYSGPGGISKPWGSVNSMHLDGGRIVLDLRKPALVKSAFIIAPRDGMGHRLVVDLAETTREAFLAAAGSGPA
SARAPVLKPPAAAKAVAPVPAAAKPSPAPVLVPPKPAEEFHLPEAAPAEAARPATAPSASRIVVNSPNAPAESIVPPTEP
VTLVPPPPSQVPVPVERPQLAEKPAVEKGARAQAATPMLSAPMPPPPERPQPVAAPQPVAAPQPPVPQPPAPVQQQAAPV
PTPSMESVAKAKDGVPVIVIDPGHGGVDPGATGVSGTYEKHITLAMARELKAMLERNGRYRVHLTRDRDVFIRLRERIAI
ARAQGADLFISLHADAVQSPQIRGLSVYTLSRNASDAEAQALAEKENKADLIAGIDLTHESADVANILIDLAQRETMNRS
AGFATELVDEVGQEMDLLGNTHRFAGFAVLKAPDVPAVLVEMGYLSNESEEKMLRQPQYRARLAKSIAKAVERFFPPNLK
AKRP

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=222, Percent_Identity=40.990990990991, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1788776, Length=228, Percent_Identity=40.7894736842105, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1790611, Length=245, Percent_Identity=35.1020408163265, Blast_Score=146, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 59582; Mature: 59582

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPWRPVTVRQMRTRARLWPVLAALVLTLTSFGLGGVTASEAATASGARLGIHGEGVTRF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEECCCCCEEE
VLDLSDQVAFKITPLAEPYRIAIDLSGADYSGPGGISKPWGSVNSMHLDGGRIVLDLRKP
EEECCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCEEEEEECCC
ALVKSAFIIAPRDGMGHRLVVDLAETTREAFLAAAGSGPASARAPVLKPPAAAKAVAPVP
CEEEEEEEEECCCCCCCEEEEEHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHCCCCCC
AAAKPSPAPVLVPPKPAEEFHLPEAAPAEAARPATAPSASRIVVNSPNAPAESIVPPTEP
CCCCCCCCEEEECCCCHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHCCCCCCC
VTLVPPPPSQVPVPVERPQLAEKPAVEKGARAQAATPMLSAPMPPPPERPQPVAAPQPVA
EEEECCCCCCCCCCCCCCCCCCCCCHHCCCCCHHCCCHHCCCCCCCCCCCCCCCCCCCCC
APQPPVPQPPAPVQQQAAPVPTPSMESVAKAKDGVPVIVIDPGHGGVDPGATGVSGTYEK
CCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHHH
HITLAMARELKAMLERNGRYRVHLTRDRDVFIRLRERIAIARAQGADLFISLHADAVQSP
HHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
QIRGLSVYTLSRNASDAEAQALAEKENKADLIAGIDLTHESADVANILIDLAQRETMNRS
CCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCC
AGFATELVDEVGQEMDLLGNTHRFAGFAVLKAPDVPAVLVEMGYLSNESEEKMLRQPQYR
CCHHHHHHHHHHHHHHHHCCCCHHCEEEEEECCCCCCHHHHHHCCCCCHHHHHHHCHHHH
ARLAKSIAKAVERFFPPNLKAKRP
HHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MFPWRPVTVRQMRTRARLWPVLAALVLTLTSFGLGGVTASEAATASGARLGIHGEGVTRF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEECCCCCEEE
VLDLSDQVAFKITPLAEPYRIAIDLSGADYSGPGGISKPWGSVNSMHLDGGRIVLDLRKP
EEECCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCEEEEEECCC
ALVKSAFIIAPRDGMGHRLVVDLAETTREAFLAAAGSGPASARAPVLKPPAAAKAVAPVP
CEEEEEEEEECCCCCCCEEEEEHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHCCCCCC
AAAKPSPAPVLVPPKPAEEFHLPEAAPAEAARPATAPSASRIVVNSPNAPAESIVPPTEP
CCCCCCCCEEEECCCCHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHCCCCCCC
VTLVPPPPSQVPVPVERPQLAEKPAVEKGARAQAATPMLSAPMPPPPERPQPVAAPQPVA
EEEECCCCCCCCCCCCCCCCCCCCCHHCCCCCHHCCCHHCCCCCCCCCCCCCCCCCCCCC
APQPPVPQPPAPVQQQAAPVPTPSMESVAKAKDGVPVIVIDPGHGGVDPGATGVSGTYEK
CCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHHH
HITLAMARELKAMLERNGRYRVHLTRDRDVFIRLRERIAIARAQGADLFISLHADAVQSP
HHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
QIRGLSVYTLSRNASDAEAQALAEKENKADLIAGIDLTHESADVANILIDLAQRETMNRS
CCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCC
AGFATELVDEVGQEMDLLGNTHRFAGFAVLKAPDVPAVLVEMGYLSNESEEKMLRQPQYR
CCHHHHHHHHHHHHHHHHCCCCHHCEEEEEECCCCCCHHHHHHCCCCCHHHHHHHCHHHH
ARLAKSIAKAVERFFPPNLKAKRP
HHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]