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The map label for this gene is tolB

Identifier: 83312310

GI number: 83312310

Start: 3478597

End: 3479940

Strand: Reverse

Name: tolB

Synonym: amb3211

Alternate gene names: 83312310

Gene position: 3479940-3478597 (Counterclockwise)

Preceding gene: 83312311

Following gene: 83312309

Centisome position: 70.06

GC content: 64.81

Gene sequence:

>1344_bases
ATGATCACCATGTCACGCATTCGCTCGCTCGCGGCGTTCGCCGTCTTCGTCATCCTCGGGGTTGCGGCGGTCTTGCCCGC
CCAGGCCGAGGTGCGGATCGACATCACCCGCGGCCAGATGAAGCCGCTGCCCATCGCCATTCCCGATTTCGCCGGTGCGT
CGGCCCAGGACAACCGCATGGGCGCCGACATCGCCCGGGTGGTCTCCGCCGATCTCGAACGGTCGGGCCTGTTCAAGCCC
ATCGATCCCAAGGCCTTCATCCAGAACGCCGCGTCCTTGCAGGCCGGACCGCGCTTCCCCGACTGGAAGGCCATCAATGC
CGAGGCCCTGGTCAGCGGCAAGATCGAAAGCGGCGCCGACGGCCGTGTCCGGGTGGAGTTCCGCCTGTGGGACGTGTTCA
ACGAAGCCTATATGACCGGCTGGACGCTGTCCGCCTCGCCCCAGGATTGGCGCCGCCTGTCCCACAAGGTGGCCGACGCC
ATCTACAAGCGGGTGACCGGCGAGGACGGCTATTTCGACACCCAGATCGTCTATATCGCGGAATCCGGTCCCAAGAACGA
CCGCAAGAAGCGCCTGTCCATCATGGACCAGGATGGCGAGAACCACCGGTTCCTCACCGACGGCTCGGAACTGGTGCTGA
CGCCCCGCTTCTCGCCCAGCGCCCGCGAGATCACCTATCTGTCGTATTTCAAGGGCGTGCCCCGGGTCTACATCTTCAAC
CTGGACACCGGCCGGCGGGAATCGCTGGGCAATTTCCCGGGCATGACCTTCGCGCCGCGCTTCTCGCCAGACGGCAACAA
GGTCGTCTTCAGCATGGCCGAGAACGGCAACACCGAAATTTACGAGATGAACCTGCTGACGCGCCAGAAGCGCCAGCTGA
CCATGAACCCGGCCATCGACACGGCACCCAGCTATTCGCCCGACGGCCAGCAGATCGTCTTCGAATCTGATCGTGGCGGC
GGGCAGCAGATCTACACCATGAGTGCCGACGGCTCGAACCCGCAGCGCATCAGCTTCGGCGACGGCCGCTACGGCACCCC
GGTATGGAGCCCGCGCGGCGATCTCATTGCTTTCACCAAGCAGAAGGGTGGGCAATTCTTCATAGGTGTCATGCGCACCG
ACGGCTCGGGCGAGCGCCTGCTGGCCGAGGGCTTCCTGGTGGAAGGCCCCACCTGGGCGCCCAACGGCCGGGTTCTGTCC
TTCTTCCGGGAATCTCCTTCGGATGAGCGCGGTCGGGGCCAGGTGGTGCGCCTTTACACCATCGATCTGACCGGCGTGAA
TGAAAGAGTGCTGCTGACGCCCGTCGACGGCTCGGATCCCGCGTGGTCCCCCTTGATTCCCTAG

Upstream 100 bases:

>100_bases
GCGCCGTGCGCGCCGCCAGCCCGCTGCCCATTCCGGTGGACAAGTACGACCAGTTCAGGGATTTCACTTTGGCCTTCAAC
CCCAAGTTTGCGGCAGGGAG

Downstream 100 bases:

>100_bases
AATTATCTTTGTATAGTCCGCGCCGATTAGGATTTAATCCGGTGACATCGGGTCCGATCGCTAACGATGTCACTGGTGGG
GCGTGTCGTCCTTGATTGGG

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MITMSRIRSLAAFAVFVILGVAAVLPAQAEVRIDITRGQMKPLPIAIPDFAGASAQDNRMGADIARVVSADLERSGLFKP
IDPKAFIQNAASLQAGPRFPDWKAINAEALVSGKIESGADGRVRVEFRLWDVFNEAYMTGWTLSASPQDWRRLSHKVADA
IYKRVTGEDGYFDTQIVYIAESGPKNDRKKRLSIMDQDGENHRFLTDGSELVLTPRFSPSAREITYLSYFKGVPRVYIFN
LDTGRRESLGNFPGMTFAPRFSPDGNKVVFSMAENGNTEIYEMNLLTRQKRQLTMNPAIDTAPSYSPDGQQIVFESDRGG
GQQIYTMSADGSNPQRISFGDGRYGTPVWSPRGDLIAFTKQKGGQFFIGVMRTDGSGERLLAEGFLVEGPTWAPNGRVLS
FFRESPSDERGRGQVVRLYTIDLTGVNERVLLTPVDGSDPAWSPLIP

Sequences:

>Translated_447_residues
MITMSRIRSLAAFAVFVILGVAAVLPAQAEVRIDITRGQMKPLPIAIPDFAGASAQDNRMGADIARVVSADLERSGLFKP
IDPKAFIQNAASLQAGPRFPDWKAINAEALVSGKIESGADGRVRVEFRLWDVFNEAYMTGWTLSASPQDWRRLSHKVADA
IYKRVTGEDGYFDTQIVYIAESGPKNDRKKRLSIMDQDGENHRFLTDGSELVLTPRFSPSAREITYLSYFKGVPRVYIFN
LDTGRRESLGNFPGMTFAPRFSPDGNKVVFSMAENGNTEIYEMNLLTRQKRQLTMNPAIDTAPSYSPDGQQIVFESDRGG
GQQIYTMSADGSNPQRISFGDGRYGTPVWSPRGDLIAFTKQKGGQFFIGVMRTDGSGERLLAEGFLVEGPTWAPNGRVLS
FFRESPSDERGRGQVVRLYTIDLTGVNERVLLTPVDGSDPAWSPLIP
>Mature_447_residues
MITMSRIRSLAAFAVFVILGVAAVLPAQAEVRIDITRGQMKPLPIAIPDFAGASAQDNRMGADIARVVSADLERSGLFKP
IDPKAFIQNAASLQAGPRFPDWKAINAEALVSGKIESGADGRVRVEFRLWDVFNEAYMTGWTLSASPQDWRRLSHKVADA
IYKRVTGEDGYFDTQIVYIAESGPKNDRKKRLSIMDQDGENHRFLTDGSELVLTPRFSPSAREITYLSYFKGVPRVYIFN
LDTGRRESLGNFPGMTFAPRFSPDGNKVVFSMAENGNTEIYEMNLLTRQKRQLTMNPAIDTAPSYSPDGQQIVFESDRGG
GQQIYTMSADGSNPQRISFGDGRYGTPVWSPRGDLIAFTKQKGGQFFIGVMRTDGSGERLLAEGFLVEGPTWAPNGRVLS
FFRESPSDERGRGQVVRLYTIDLTGVNERVLLTPVDGSDPAWSPLIP

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=437, Percent_Identity=30.2059496567506, Blast_Score=178, Evalue=6e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_MAGSA (Q2W2B0)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_422574.1
- ProteinModelPortal:   Q2W2B0
- SMR:   Q2W2B0
- STRING:   Q2W2B0
- GeneID:   3805300
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb3211
- NMPDR:   fig|342108.5.peg.2881
- eggNOG:   COG0823
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- PhylomeDB:   Q2W2B0
- ProtClustDB:   PRK05137
- BioCyc:   MMAG342108:AMB3211-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 49414; Mature: 49414

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS00152 ATPASE_ALPHA_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITMSRIRSLAAFAVFVILGVAAVLPAQAEVRIDITRGQMKPLPIAIPDFAGASAQDNRM
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEECCCCCCCCCCCCCC
GADIARVVSADLERSGLFKPIDPKAFIQNAASLQAGPRFPDWKAINAEALVSGKIESGAD
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCEECCCCEEEECCCCCCCC
GRVRVEFRLWDVFNEAYMTGWTLSASPQDWRRLSHKVADAIYKRVTGEDGYFDTQIVYIA
CEEEEEEEEEEHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
ESGPKNDRKKRLSIMDQDGENHRFLTDGSELVLTPRFSPSAREITYLSYFKGVPRVYIFN
CCCCCCCHHHHEEEECCCCCCCEEEECCCEEEEEECCCCCCCEEEEEHHHCCCCEEEEEE
LDTGRRESLGNFPGMTFAPRFSPDGNKVVFSMAENGNTEIYEMNLLTRQKRQLTMNPAID
CCCCCHHHCCCCCCCEECCCCCCCCCEEEEEEECCCCCEEEEEEHHHHHHHHEEECCCCC
TAPSYSPDGQQIVFESDRGGGQQIYTMSADGSNPQRISFGDGRYGTPVWSPRGDLIAFTK
CCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCEEEEEE
QKGGQFFIGVMRTDGSGERLLAEGFLVEGPTWAPNGRVLSFFRESPSDERGRGQVVRLYT
CCCCEEEEEEEEECCCCCEEEECCEEEECCCCCCCCCEEEEECCCCCCCCCCCCEEEEEE
IDLTGVNERVLLTPVDGSDPAWSPLIP
EEECCCCCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MITMSRIRSLAAFAVFVILGVAAVLPAQAEVRIDITRGQMKPLPIAIPDFAGASAQDNRM
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEECCCCCCCCCCCCCC
GADIARVVSADLERSGLFKPIDPKAFIQNAASLQAGPRFPDWKAINAEALVSGKIESGAD
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCEECCCCEEEECCCCCCCC
GRVRVEFRLWDVFNEAYMTGWTLSASPQDWRRLSHKVADAIYKRVTGEDGYFDTQIVYIA
CEEEEEEEEEEHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
ESGPKNDRKKRLSIMDQDGENHRFLTDGSELVLTPRFSPSAREITYLSYFKGVPRVYIFN
CCCCCCCHHHHEEEECCCCCCCEEEECCCEEEEEECCCCCCCEEEEEHHHCCCCEEEEEE
LDTGRRESLGNFPGMTFAPRFSPDGNKVVFSMAENGNTEIYEMNLLTRQKRQLTMNPAID
CCCCCHHHCCCCCCCEECCCCCCCCCEEEEEEECCCCCEEEEEEHHHHHHHHEEECCCCC
TAPSYSPDGQQIVFESDRGGGQQIYTMSADGSNPQRISFGDGRYGTPVWSPRGDLIAFTK
CCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCEEEEEE
QKGGQFFIGVMRTDGSGERLLAEGFLVEGPTWAPNGRVLSFFRESPSDERGRGQVVRLYT
CCCCEEEEEEEEECCCCCEEEECCEEEECCCCCCCCCEEEEECCCCCCCCCCCCEEEEEE
IDLTGVNERVLLTPVDGSDPAWSPLIP
EEECCCCCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA