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The map label for this gene is tilS [H]

Identifier: 83312306

GI number: 83312306

Start: 3474758

End: 3476089

Strand: Reverse

Name: tilS [H]

Synonym: amb3207

Alternate gene names: 83312306

Gene position: 3476089-3474758 (Counterclockwise)

Preceding gene: 83312307

Following gene: 83312305

Centisome position: 69.98

GC content: 73.95

Gene sequence:

>1332_bases
ATGTCCGTTCCGCCGCTTTCCTCCGACGACTTCTCCCGCCTGATGGCGCCCCTGGGGCCGTTCGAGGCCGCACCGCGTCT
GGCGGTGGCGGTGTCGGGCGGCGCCGATTCCCTGGCCCTGGCCCTGCTGGCGGCGGATTGGGCGAAGGCGCGGGGCGGCG
AGGTTCTGGCCTTGACCGTCGATCACCGTCTGCGCCCCGAATCCGCCGCCGAGGCGGCCCGGGTGGGGGCGTGGCTGGCC
CGTGACGGCATCTCTCACCGCATTCTCGCCTGGGAGGGTGACAAGCCCGCCGCCGATCTTCAGGCGGCGGCCCGCGCGGC
GCGCTATGGCCTGCTGGGCGAGGCCTGCGCCGCCGAGGGCATTCTGCATCTGGCCCTGGCCCATCATCAGGATGATCAGG
CGGAGACGCTGCTGCTGCGCCTCGGCCGGGGGTCGGGGCTGGAAGGGCTGGGGGCCATGGCGGCCGAGCGTCCGACCTCC
TGGGGGCGCCTGCTGCGCCCGCTGTTGCCCGTGCCGCGTGTCCGTCTGGAAGCCACCCTGCGGCACAGGGGGCAGGAGTG
GACCAGCGATCCCTCCAACCGCAACCCCGCCTTCGCCCGGGTGCGGCTGCGCCATCTGGCTGGGGATCTGGCCGCCGAGG
GGATGACGCCACGCCGGCTGGCCGAGACCGCCGGGCGGCTGGCCCGGGCCCAGGCGGCCGTCGATTCCATGGTGGCCGAG
GCCGCCGCCCGGCATGTGACCCTGGACCCCGCCGGCTTCGGCCGGCTGCGCCCCGAGGCCCTGGCCGGATTGCCCGACGA
GGTGGGGCTGCGCCTGCTGGCCGGCCTGCTGCTGAGCGTGGGCGGCGAGGCCCATACGCCGCGCCTGGAACGGCTGGAAC
GCCTTCACGCCACGCTGTGCGGAGGATTGGAGGCGGCGCGGACCCTGGCGGGCTGCCGCATCGTGCCCCAGGGCGATTCC
GTGGTGCTGTGCCGTGAGCCCGCCCGGGTGGCGCCGCCGCTGGATCTGGTTCCGGGAAGCCGCATCCGCTGGGATGGGCG
CTTCGAGGCGGTGGTGGCCGGGGACGCGCCGCCGGGCTTGCGCCTGGGGGCGCTGGGGCCCGAAGGGCGGCGCAAGGTGC
TGGCGGCCATGGGACGGCAGCGGCCCGAACCGGTTCCCACCTGCGCCATTCCCACTTTACCGGCCCTTTACCATGAGCGG
GGTATTCTCGCGGCGCCGCACCTCGGCTATAACCGGGAGGGTGCCGGTCGGGTCCTGGTGAGCTTGCGCGCTTCGCCGCT
CCATGTTCTGACCGTTTTCGCTTGTGCCGGCGGGAATCGGCATTATCTGTAG

Upstream 100 bases:

>100_bases
CTTGGACCCCAAACCCCATTCCTGAAATAAAATGGGGTCCGGGGCATTGGCCCCGGATGGGTTCGGGCGAGTGCCCGACA
GGTCGGGGACACCAGAACCC

Downstream 100 bases:

>100_bases
ACGACGTTGTCCGGTACAGCACTTGACGGGAAGGACGCTACCTTGAATTTTAGCAAGAACCTGGCGTTGTGGGTCATCAT
CGCCGTGTTGCTGGTGATGC

Product: ATPase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MSVPPLSSDDFSRLMAPLGPFEAAPRLAVAVSGGADSLALALLAADWAKARGGEVLALTVDHRLRPESAAEAARVGAWLA
RDGISHRILAWEGDKPAADLQAAARAARYGLLGEACAAEGILHLALAHHQDDQAETLLLRLGRGSGLEGLGAMAAERPTS
WGRLLRPLLPVPRVRLEATLRHRGQEWTSDPSNRNPAFARVRLRHLAGDLAAEGMTPRRLAETAGRLARAQAAVDSMVAE
AAARHVTLDPAGFGRLRPEALAGLPDEVGLRLLAGLLLSVGGEAHTPRLERLERLHATLCGGLEAARTLAGCRIVPQGDS
VVLCREPARVAPPLDLVPGSRIRWDGRFEAVVAGDAPPGLRLGALGPEGRRKVLAAMGRQRPEPVPTCAIPTLPALYHER
GILAAPHLGYNREGAGRVLVSLRASPLHVLTVFACAGGNRHYL

Sequences:

>Translated_443_residues
MSVPPLSSDDFSRLMAPLGPFEAAPRLAVAVSGGADSLALALLAADWAKARGGEVLALTVDHRLRPESAAEAARVGAWLA
RDGISHRILAWEGDKPAADLQAAARAARYGLLGEACAAEGILHLALAHHQDDQAETLLLRLGRGSGLEGLGAMAAERPTS
WGRLLRPLLPVPRVRLEATLRHRGQEWTSDPSNRNPAFARVRLRHLAGDLAAEGMTPRRLAETAGRLARAQAAVDSMVAE
AAARHVTLDPAGFGRLRPEALAGLPDEVGLRLLAGLLLSVGGEAHTPRLERLERLHATLCGGLEAARTLAGCRIVPQGDS
VVLCREPARVAPPLDLVPGSRIRWDGRFEAVVAGDAPPGLRLGALGPEGRRKVLAAMGRQRPEPVPTCAIPTLPALYHER
GILAAPHLGYNREGAGRVLVSLRASPLHVLTVFACAGGNRHYL
>Mature_442_residues
SVPPLSSDDFSRLMAPLGPFEAAPRLAVAVSGGADSLALALLAADWAKARGGEVLALTVDHRLRPESAAEAARVGAWLAR
DGISHRILAWEGDKPAADLQAAARAARYGLLGEACAAEGILHLALAHHQDDQAETLLLRLGRGSGLEGLGAMAAERPTSW
GRLLRPLLPVPRVRLEATLRHRGQEWTSDPSNRNPAFARVRLRHLAGDLAAEGMTPRRLAETAGRLARAQAAVDSMVAEA
AARHVTLDPAGFGRLRPEALAGLPDEVGLRLLAGLLLSVGGEAHTPRLERLERLHATLCGGLEAARTLAGCRIVPQGDSV
VLCREPARVAPPLDLVPGSRIRWDGRFEAVVAGDAPPGLRLGALGPEGRRKVLAAMGRQRPEPVPTCAIPTLPALYHERG
ILAAPHLGYNREGAGRVLVSLRASPLHVLTVFACAGGNRHYL

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=184, Percent_Identity=34.2391304347826, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 46823; Mature: 46691

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPPLSSDDFSRLMAPLGPFEAAPRLAVAVSGGADSLALALLAADWAKARGGEVLALTV
CCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEE
DHRLRPESAAEAARVGAWLARDGISHRILAWEGDKPAADLQAAARAARYGLLGEACAAEG
CCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHH
ILHLALAHHQDDQAETLLLRLGRGSGLEGLGAMAAERPTSWGRLLRPLLPVPRVRLEATL
HHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHH
RHRGQEWTSDPSNRNPAFARVRLRHLAGDLAAEGMTPRRLAETAGRLARAQAAVDSMVAE
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AAARHVTLDPAGFGRLRPEALAGLPDEVGLRLLAGLLLSVGGEAHTPRLERLERLHATLC
HHHCEEEECCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
GGLEAARTLAGCRIVPQGDSVVLCREPARVAPPLDLVPGSRIRWDGRFEAVVAGDAPPGL
CCHHHHHHHHCCEEECCCCCEEEECCCCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCC
RLGALGPEGRRKVLAAMGRQRPEPVPTCAIPTLPALYHERGILAAPHLGYNREGAGRVLV
EEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCEECCCCCCCCCCCCEEEE
SLRASPLHVLTVFACAGGNRHYL
EECCCCCEEEEEEEECCCCCCCC
>Mature Secondary Structure 
SVPPLSSDDFSRLMAPLGPFEAAPRLAVAVSGGADSLALALLAADWAKARGGEVLALTV
CCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEE
DHRLRPESAAEAARVGAWLARDGISHRILAWEGDKPAADLQAAARAARYGLLGEACAAEG
CCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHH
ILHLALAHHQDDQAETLLLRLGRGSGLEGLGAMAAERPTSWGRLLRPLLPVPRVRLEATL
HHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHH
RHRGQEWTSDPSNRNPAFARVRLRHLAGDLAAEGMTPRRLAETAGRLARAQAAVDSMVAE
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AAARHVTLDPAGFGRLRPEALAGLPDEVGLRLLAGLLLSVGGEAHTPRLERLERLHATLC
HHHCEEEECCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
GGLEAARTLAGCRIVPQGDSVVLCREPARVAPPLDLVPGSRIRWDGRFEAVVAGDAPPGL
CCHHHHHHHHCCEEECCCCCEEEECCCCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCC
RLGALGPEGRRKVLAAMGRQRPEPVPTCAIPTLPALYHERGILAAPHLGYNREGAGRVLV
EEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCEECCCCCCCCCCCCEEEE
SLRASPLHVLTVFACAGGNRHYL
EECCCCCEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA