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The map label for this gene is pyk [H]

Identifier: 83312273

GI number: 83312273

Start: 3435376

End: 3436803

Strand: Reverse

Name: pyk [H]

Synonym: amb3174

Alternate gene names: 83312273

Gene position: 3436803-3435376 (Counterclockwise)

Preceding gene: 83312274

Following gene: 83312269

Centisome position: 69.19

GC content: 65.34

Gene sequence:

>1428_bases
ATGAGACAGAAGCGCAATACTCGTATCGTCGCCACCCTGGGGCCTGCCAGTTCCAGCCCGGACCAGATCAGGGCGCTGTG
GGAGGCCGGGGCGGACGTCTTTCGCCTCAATTTCAGCCATGGCAGTCATGCCGACCACCTGGAGCGGTTCAAGGAAATCC
GCCGCCTGGAGGCCGAACTGGGCCGTCCCATCGCCATCCTTGCCGATTTGCAAGGTCCGAAGCTGCGTCTTGGAGTCTTC
AAGGCGGGCAAGGTGGCTCTCAAACCAGGGCAGGCTTTCCGCCTCGACCTGTCGCGGGAACCGGGGGATATCAACCGCGC
CCCGCTGCCTCACCCGGAAATCCTGTCGGCCCTGACGCCGGGTTCGGACCTTTTGCTGGATGACGGCAAGGTTCGGCTGC
GGGTCGAATCCTGCGGGCCAGACTTTGCCGAAACCGTGGTGGTGGTGGCGGGTCCCTTGTCCGACCGCAAGGGCGTCAAT
GTGCCCGACGCGGTGATCCCCCTGTCGGCCCTGACCGAGAAGGACCGCCGCGACCTTGCCTTCGCCCTGGAATTGGGCAT
CGACTGGATCGCCCTGTCCTTCGTGCAACGGCCCGAGGATGTGGAGGAGGCCCGCGCCCTGATCGGCGACCGCGCCCGCA
TTCTGTCCAAGTTGGAGAAGCCCTCCGCCATCGACCGCCTCGATGAGATCGTTGCTCTGTCCGACGCGGTGATGGTGGCG
CGCGGCGATCTGGGCGTCGAATTGCCGCCCGAGGATGTGCCCGGCCTGCAAAAACGCATCATCCGCTCATGTCGCGCTGC
CGGTAAGCCGGTGGTCGTTGCCACCCAGATGTTGGAATCCATGATAGAGGCGCCCACACCGACGCGGGCCGAAGCCTCCG
ACGTGGCCACCGCCGTCTTCGCCGGTGCCGACGCGGTGATGCTCTCGGCGGAATCCGCCTCGGGCCGCTATCCCGTCGAA
GCCGTCACCATGATGAGCCGCATCATCAGCCGGGTTGAGAGCGATCCCAACTATCTCGACGGTCTGGGCAGCGGTTGCGC
CAAGCCAGAGGCCAACGACGCCGACGCCATCAGCGCGGCCGTCTGCCAGGTGACCGGCATCCTGTCTGTCGCCGCCATCG
TCGCCTACACCACTTCGGGATTCACCACGCTGCGCATCGCCCGAGAGCGTCCGGCTTCCCCGATCCTCAGCTTGACCCCG
AATGCGGCCACTGCGCGCCACGTTGCTTTGGCTTGGGGCGTCCATTCCGTCCACACCGAAGATGCCGCCAATGTGGAGGA
CATGGTCGCCAAGGCGTCAGGCATCGCCACCAGAGAGGGTTTCACCGAGGGCGGCAAGCCACTGGTGATCATCGCAGGCA
TGCCATTCGGAACTGCCGGTTCAACCAACCTGTTGCGCATCGTGCGCGTGCCCAGGGCTTCACTCTGA

Upstream 100 bases:

>100_bases
GCTTTTTCCATGCCTTGGGCGATTCCGTGGTGACCGGCCCGACACTCACCAACGTCAACGATTTCCGCGCGATTTTGATC
GCCGACAAGGACAGCACTCC

Downstream 100 bases:

>100_bases
CCAAACGCAATGAAATTTGCGGCTCCTCCCGCCGCACTGACCCTGGTTCGGGTCAAACTAAGGGCTGCCCATGAAAATGG
GTGGCCCTTTCTTCATGATT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MRQKRNTRIVATLGPASSSPDQIRALWEAGADVFRLNFSHGSHADHLERFKEIRRLEAELGRPIAILADLQGPKLRLGVF
KAGKVALKPGQAFRLDLSREPGDINRAPLPHPEILSALTPGSDLLLDDGKVRLRVESCGPDFAETVVVVAGPLSDRKGVN
VPDAVIPLSALTEKDRRDLAFALELGIDWIALSFVQRPEDVEEARALIGDRARILSKLEKPSAIDRLDEIVALSDAVMVA
RGDLGVELPPEDVPGLQKRIIRSCRAAGKPVVVATQMLESMIEAPTPTRAEASDVATAVFAGADAVMLSAESASGRYPVE
AVTMMSRIISRVESDPNYLDGLGSGCAKPEANDADAISAAVCQVTGILSVAAIVAYTTSGFTTLRIARERPASPILSLTP
NAATARHVALAWGVHSVHTEDAANVEDMVAKASGIATREGFTEGGKPLVIIAGMPFGTAGSTNLLRIVRVPRASL

Sequences:

>Translated_475_residues
MRQKRNTRIVATLGPASSSPDQIRALWEAGADVFRLNFSHGSHADHLERFKEIRRLEAELGRPIAILADLQGPKLRLGVF
KAGKVALKPGQAFRLDLSREPGDINRAPLPHPEILSALTPGSDLLLDDGKVRLRVESCGPDFAETVVVVAGPLSDRKGVN
VPDAVIPLSALTEKDRRDLAFALELGIDWIALSFVQRPEDVEEARALIGDRARILSKLEKPSAIDRLDEIVALSDAVMVA
RGDLGVELPPEDVPGLQKRIIRSCRAAGKPVVVATQMLESMIEAPTPTRAEASDVATAVFAGADAVMLSAESASGRYPVE
AVTMMSRIISRVESDPNYLDGLGSGCAKPEANDADAISAAVCQVTGILSVAAIVAYTTSGFTTLRIARERPASPILSLTP
NAATARHVALAWGVHSVHTEDAANVEDMVAKASGIATREGFTEGGKPLVIIAGMPFGTAGSTNLLRIVRVPRASL
>Mature_475_residues
MRQKRNTRIVATLGPASSSPDQIRALWEAGADVFRLNFSHGSHADHLERFKEIRRLEAELGRPIAILADLQGPKLRLGVF
KAGKVALKPGQAFRLDLSREPGDINRAPLPHPEILSALTPGSDLLLDDGKVRLRVESCGPDFAETVVVVAGPLSDRKGVN
VPDAVIPLSALTEKDRRDLAFALELGIDWIALSFVQRPEDVEEARALIGDRARILSKLEKPSAIDRLDEIVALSDAVMVA
RGDLGVELPPEDVPGLQKRIIRSCRAAGKPVVVATQMLESMIEAPTPTRAEASDVATAVFAGADAVMLSAESASGRYPVE
AVTMMSRIISRVESDPNYLDGLGSGCAKPEANDADAISAAVCQVTGILSVAAIVAYTTSGFTTLRIARERPASPILSLTP
NAATARHVALAWGVHSVHTEDAANVEDMVAKASGIATREGFTEGGKPLVIIAGMPFGTAGSTNLLRIVRVPRASL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=495, Percent_Identity=39.1919191919192, Blast_Score=315, Evalue=4e-86,
Organism=Homo sapiens, GI33286422, Length=491, Percent_Identity=38.4928716904277, Blast_Score=315, Evalue=5e-86,
Organism=Homo sapiens, GI33286420, Length=491, Percent_Identity=38.4928716904277, Blast_Score=315, Evalue=5e-86,
Organism=Homo sapiens, GI10835121, Length=495, Percent_Identity=37.979797979798, Blast_Score=291, Evalue=9e-79,
Organism=Homo sapiens, GI32967597, Length=490, Percent_Identity=38.1632653061225, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=39.7212543554007, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=39.7212543554007, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=36.2869198312236, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=36.2869198312236, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=35.0467289719626, Blast_Score=132, Evalue=6e-31,
Organism=Escherichia coli, GI1788160, Length=483, Percent_Identity=40.3726708074534, Blast_Score=312, Evalue=2e-86,
Organism=Escherichia coli, GI1787965, Length=472, Percent_Identity=36.4406779661017, Blast_Score=291, Evalue=6e-80,
Organism=Caenorhabditis elegans, GI17544584, Length=492, Percent_Identity=36.9918699186992, Blast_Score=297, Evalue=8e-81,
Organism=Caenorhabditis elegans, GI71984413, Length=484, Percent_Identity=37.8099173553719, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17506829, Length=484, Percent_Identity=37.8099173553719, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI71984406, Length=484, Percent_Identity=37.8099173553719, Blast_Score=280, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17506831, Length=484, Percent_Identity=37.8099173553719, Blast_Score=279, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6324923, Length=487, Percent_Identity=34.7022587268994, Blast_Score=245, Evalue=9e-66,
Organism=Saccharomyces cerevisiae, GI6319279, Length=486, Percent_Identity=34.156378600823, Blast_Score=243, Evalue=7e-65,
Organism=Drosophila melanogaster, GI24648964, Length=490, Percent_Identity=37.1428571428571, Blast_Score=288, Evalue=8e-78,
Organism=Drosophila melanogaster, GI28571814, Length=490, Percent_Identity=37.1428571428571, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24648966, Length=408, Percent_Identity=35.7843137254902, Blast_Score=245, Evalue=5e-65,
Organism=Drosophila melanogaster, GI24581235, Length=486, Percent_Identity=32.0987654320988, Blast_Score=242, Evalue=3e-64,
Organism=Drosophila melanogaster, GI24646914, Length=273, Percent_Identity=36.996336996337, Blast_Score=168, Evalue=9e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50454; Mature: 50454

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQKRNTRIVATLGPASSSPDQIRALWEAGADVFRLNFSHGSHADHLERFKEIRRLEAEL
CCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
GRPIAILADLQGPKLRLGVFKAGKVALKPGQAFRLDLSREPGDINRAPLPHPEILSALTP
CCCEEEEEECCCCEEEEEEEECCCEEECCCCEEEEECCCCCCCCCCCCCCCHHHHHHCCC
GSDLLLDDGKVRLRVESCGPDFAETVVVVAGPLSDRKGVNVPDAVIPLSALTEKDRRDLA
CCCEEEECCEEEEEEECCCCCHHHEEEEEECCCCCCCCCCCCHHHHCHHHHHCCHHHHHE
FALELGIDWIALSFVQRPEDVEEARALIGDRARILSKLEKPSAIDRLDEIVALSDAVMVA
EHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEE
RGDLGVELPPEDVPGLQKRIIRSCRAAGKPVVVATQMLESMIEAPTPTRAEASDVATAVF
ECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCHHHHHHHHH
AGADAVMLSAESASGRYPVEAVTMMSRIISRVESDPNYLDGLGSGCAKPEANDADAISAA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCHHHHHHH
VCQVTGILSVAAIVAYTTSGFTTLRIARERPASPILSLTPNAATARHVALAWGVHSVHTE
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCHHHHHHEECCHHHCCCC
DAANVEDMVAKASGIATREGFTEGGKPLVIIAGMPFGTAGSTNLLRIVRVPRASL
CCCCHHHHHHHHCCCHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCC
>Mature Secondary Structure
MRQKRNTRIVATLGPASSSPDQIRALWEAGADVFRLNFSHGSHADHLERFKEIRRLEAEL
CCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
GRPIAILADLQGPKLRLGVFKAGKVALKPGQAFRLDLSREPGDINRAPLPHPEILSALTP
CCCEEEEEECCCCEEEEEEEECCCEEECCCCEEEEECCCCCCCCCCCCCCCHHHHHHCCC
GSDLLLDDGKVRLRVESCGPDFAETVVVVAGPLSDRKGVNVPDAVIPLSALTEKDRRDLA
CCCEEEECCEEEEEEECCCCCHHHEEEEEECCCCCCCCCCCCHHHHCHHHHHCCHHHHHE
FALELGIDWIALSFVQRPEDVEEARALIGDRARILSKLEKPSAIDRLDEIVALSDAVMVA
EHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEE
RGDLGVELPPEDVPGLQKRIIRSCRAAGKPVVVATQMLESMIEAPTPTRAEASDVATAVF
ECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCHHHHHHHHH
AGADAVMLSAESASGRYPVEAVTMMSRIISRVESDPNYLDGLGSGCAKPEANDADAISAA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCHHHHHHH
VCQVTGILSVAAIVAYTTSGFTTLRIARERPASPILSLTPNAATARHVALAWGVHSVHTE
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCHHHHHHEECCHHHCCCC
DAANVEDMVAKASGIATREGFTEGGKPLVIIAGMPFGTAGSTNLLRIVRVPRASL
CCCCHHHHHHHHCCCHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA