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The map label for this gene is rpoA

Identifier: 83312207

GI number: 83312207

Start: 3363513

End: 3364529

Strand: Reverse

Name: rpoA

Synonym: amb3108

Alternate gene names: 83312207

Gene position: 3364529-3363513 (Counterclockwise)

Preceding gene: 83312208

Following gene: 83312206

Centisome position: 67.74

GC content: 61.55

Gene sequence:

>1017_bases
GTGATTCAGAAGAATTGGCAGGAACTGATTAAGCCCAACAAGCTCCATGTGGAGCCCGGGGCCGATCCGCAGCGTACCGC
CATCGTGGTCGCCGAACCGCTTGAGCGCGGCTTTGGTATGACGCTTGGCAATTCCTTGCGCCGCGTGCTGCTGTCGTCGC
TGCAGGGTGCCGCCGTCACCGCCATCCAGATCGACGGCGTTCTGCACGAATTCTCGTCGATCCCCGGCGTGCGTGAGGAC
GTCACCGACATCATCCTCAACATCAAGACCCTGGGCCTGCGCATGCATGGCGAGGGGCCGAAGCGGATGCATCTCCGGGC
CGTCGGCCCCGGCGAGGTCACCGCCGGCTTGATCGAGGTTGGTCACGACATCGAGATCATGGACCCCGAACTGGTGCTGT
GCACCCTGGACGAGGGCGCCAAGCTGAACATCGAGTTCACGGTCGAGACCGGCAAGGGCTATGTCCCCGCCTCGCAGAAC
CGTCCCGAGGATTCGCCGATCGGTCTGATCCCGATCGACGCCATTTTCTCGCCGGTGCGCAAGGTGGCCTACAAGGTGGA
AAACACCCGCGTGGGCCAAGTCACCGATTATGACAAGCTGTCCATGACCGTCGAGACCAACGGCGCGGTCACGCCGGATG
ATGCGGTGGCCCTGGCCGCCCGCATCCTGCAGGACCAGCTGCAGCTGTTCATCAACTTCGAGGAGCCCACCGCGGTCGTC
GAGGAAGAGAAGAAGGACGAACTGCCCTTCAACAAGAACCTGCTGCGCAAGGTGGACGAGCTGGAGCTTTCGGTCCGTTC
GGCCAACTGCCTGAAGAACGACAACATCATCTATATCGGCGACCTGGTCCAGAAGACCGAAGCCGAGATGCTGCGCACTC
CCAATTTCGGTCGCAAGTCGCTGAACGAGATCAAGGAAGTGCTGGCGCAGATGGGTCTGCACCTGGGCATGGAAATCGCC
AATTGGCCGCCGGAGAACATCGAAGAGCTGGCCAAGAAGCTGGAAGAGCCCTACTGA

Upstream 100 bases:

>100_bases
GTTTCATTGGGATAGGGCTGCCGCCGCCTTCCTTGCGAAAGGGAAGACCGGCGGCACGCCCGTTCCCGTTTTCTTTTGTT
CCTAGCATGAGGTCACGCAC

Downstream 100 bases:

>100_bases
TCTGTTTGCGGCGCGACATTGGCCGCCTGATCGGCCCCAATGTCGCGCCGTAGGCTTTTTTAAGGACCGTTCCGGTCCGC
CCGCGCCGTTCTCTCCCTAG

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MIQKNWQELIKPNKLHVEPGADPQRTAIVVAEPLERGFGMTLGNSLRRVLLSSLQGAAVTAIQIDGVLHEFSSIPGVRED
VTDIILNIKTLGLRMHGEGPKRMHLRAVGPGEVTAGLIEVGHDIEIMDPELVLCTLDEGAKLNIEFTVETGKGYVPASQN
RPEDSPIGLIPIDAIFSPVRKVAYKVENTRVGQVTDYDKLSMTVETNGAVTPDDAVALAARILQDQLQLFINFEEPTAVV
EEEKKDELPFNKNLLRKVDELELSVRSANCLKNDNIIYIGDLVQKTEAEMLRTPNFGRKSLNEIKEVLAQMGLHLGMEIA
NWPPENIEELAKKLEEPY

Sequences:

>Translated_338_residues
MIQKNWQELIKPNKLHVEPGADPQRTAIVVAEPLERGFGMTLGNSLRRVLLSSLQGAAVTAIQIDGVLHEFSSIPGVRED
VTDIILNIKTLGLRMHGEGPKRMHLRAVGPGEVTAGLIEVGHDIEIMDPELVLCTLDEGAKLNIEFTVETGKGYVPASQN
RPEDSPIGLIPIDAIFSPVRKVAYKVENTRVGQVTDYDKLSMTVETNGAVTPDDAVALAARILQDQLQLFINFEEPTAVV
EEEKKDELPFNKNLLRKVDELELSVRSANCLKNDNIIYIGDLVQKTEAEMLRTPNFGRKSLNEIKEVLAQMGLHLGMEIA
NWPPENIEELAKKLEEPY
>Mature_338_residues
MIQKNWQELIKPNKLHVEPGADPQRTAIVVAEPLERGFGMTLGNSLRRVLLSSLQGAAVTAIQIDGVLHEFSSIPGVRED
VTDIILNIKTLGLRMHGEGPKRMHLRAVGPGEVTAGLIEVGHDIEIMDPELVLCTLDEGAKLNIEFTVETGKGYVPASQN
RPEDSPIGLIPIDAIFSPVRKVAYKVENTRVGQVTDYDKLSMTVETNGAVTPDDAVALAARILQDQLQLFINFEEPTAVV
EEEKKDELPFNKNLLRKVDELELSVRSANCLKNDNIIYIGDLVQKTEAEMLRTPNFGRKSLNEIKEVLAQMGLHLGMEIA
NWPPENIEELAKKLEEPY

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=331, Percent_Identity=49.5468277945619, Blast_Score=305, Evalue=3e-84,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_MAGSA (Q2W2L3)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_422471.1
- HSSP:   P20429
- ProteinModelPortal:   Q2W2L3
- STRING:   Q2W2L3
- GeneID:   3804455
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb3108
- NMPDR:   fig|342108.5.peg.2790
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   CHIDESA
- PhylomeDB:   Q2W2L3
- ProtClustDB:   PRK05182
- BioCyc:   MMAG342108:AMB3108-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 37454; Mature: 37454

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQKNWQELIKPNKLHVEPGADPQRTAIVVAEPLERGFGMTLGNSLRRVLLSSLQGAAVT
CCCCCHHHHCCCCEEEECCCCCCCCEEEEEECCHHCCCCCHHHHHHHHHHHHHCCCCEEE
AIQIDGVLHEFSSIPGVREDVTDIILNIKTLGLRMHGEGPKRMHLRAVGPGEVTAGLIEV
EEEEHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCHHHHHHHHC
GHDIEIMDPELVLCTLDEGAKLNIEFTVETGKGYVPASQNRPEDSPIGLIPIDAIFSPVR
CCCEEEECCCEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEHHHHHHHHH
KVAYKVENTRVGQVTDYDKLSMTVETNGAVTPDDAVALAARILQDQLQLFINFEEPTAVV
HHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEECCCCCHHHH
EEEKKDELPFNKNLLRKVDELELSVRSANCLKNDNIIYIGDLVQKTEAEMLRTPNFGRKS
HCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCHHH
LNEIKEVLAQMGLHLGMEIANWPPENIEELAKKLEEPY
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MIQKNWQELIKPNKLHVEPGADPQRTAIVVAEPLERGFGMTLGNSLRRVLLSSLQGAAVT
CCCCCHHHHCCCCEEEECCCCCCCCEEEEEECCHHCCCCCHHHHHHHHHHHHHCCCCEEE
AIQIDGVLHEFSSIPGVREDVTDIILNIKTLGLRMHGEGPKRMHLRAVGPGEVTAGLIEV
EEEEHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCHHHHHHHHC
GHDIEIMDPELVLCTLDEGAKLNIEFTVETGKGYVPASQNRPEDSPIGLIPIDAIFSPVR
CCCEEEECCCEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEHHHHHHHHH
KVAYKVENTRVGQVTDYDKLSMTVETNGAVTPDDAVALAARILQDQLQLFINFEEPTAVV
HHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEECCCCCHHHH
EEEKKDELPFNKNLLRKVDELELSVRSANCLKNDNIIYIGDLVQKTEAEMLRTPNFGRKS
HCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCHHH
LNEIKEVLAQMGLHLGMEIANWPPENIEELAKKLEEPY
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA