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The map label for this gene is norB [H]

Identifier: 83312043

GI number: 83312043

Start: 3147718

End: 3149037

Strand: Reverse

Name: norB [H]

Synonym: amb2944

Alternate gene names: 83312043

Gene position: 3149037-3147718 (Counterclockwise)

Preceding gene: 83312044

Following gene: 83312042

Centisome position: 63.4

GC content: 60.68

Gene sequence:

>1320_bases
ATGAAATATGAATCGCAAAAAGTCGCGCTCTACTTCTTTGCCGGAGCGCTTGGCCTGTTCGTCGCCCAGATTCTGTTCGG
ACTGGTGATCGGCACCATCGTCGTGATGCCCAACACCTTGTCCGAATTGCTGCCGTTCAACATCGCCCGCATGATCCACA
CCAACGCCCTGATCGTCTGGCTGTTGATGGGATTCTTCGGCGCGTCCTACTACCTGATCCCCGAGGAAGCCGAGACCGAG
CTGTACAGCACCAAGCTGGCCATCGTTCAGTTCTGGCTGTTCCTGATCGGGGCCGCCGCCGCCGTGGTGGGCTATCTGTT
CCACATTCACGAGGGACGCGAGTTCCTGGAACAGCCCCTGTGGATCAAGCTGGCCATCGTCGTCGTGGCGCTGATGTTCC
TGTTCAACATCACCATGACGGTGCTGAAGGGGCGCAAGACCGCCATCACCAACGTCCTGCTGCTGGGCTTGTGGGGCATC
GCCGTCTTCTTCCTGTTCTCGCTCTACAACCCCGCCAACCTGATCATGGACAAGATCTTCTGGTGGTGGGTCGTTCATCT
GTGGGTCGAGGGCGTGTGGGAGCTGGTCATGGCCTCCATCCTGGCCTTCCTGATGATCAAGCTGACCGGCGTCGACCGCG
AAGTGGTCGAGAAGTGGCTGTACGTCATCATCGGCCTGGCGCTGTTCTCCGGCATCCTGGGCACCGGCCACCACTACTAC
TGGATCGGCACCCCCGGCTACTGGCAGTGGATCGGCTCGGTGTTCTCCACCCTGGAAGTGGCTCCGTTCTTCGCCATGGT
GGTCTTCGCCTTCATGATGGTGTGGAAGGGCGGCCGCAACCACCCCAACAAGGCGGCTCTGCTGTGGTCGCTGGGCTGCT
CGGTCTTCGCGTTCTTCGGCGCCGGGGTGTGGGGCTTCCTGCACACGCTGGCTCCGGTCAACTACATCACCCATGGCACC
CAGGTCACGGCGGCCCACGGTCATCTGGCGTTCTACGGCGCCTATGTGATGATCAACATCGCCATCATGACCTATGCCAT
GCCCAATATCTTGAAGCGTGAGCCCTACAACCAGCTGCTCAACATGTGGGGCTTCTGGATCACCTCGTCGGGCGTGACCT
TCATGACCTTCGCGCTGACCTTCGCCGGAGTGGTGCAGAGCCATCTCCAGCGGGTGATGGGCATGGGCTTCATGGAAGTC
CAGGACCAGCTCGGCCTGTTCTACTGGATGCGCCTGGGCGCCGGTGTCGCCACCTTCATCGGTGTCGTCATGATCGTCTG
GTCGCTGGTGGTTCCCGGCAAGGCCAAGCCGGCCCAATAA

Upstream 100 bases:

>100_bases
AGGAGCTGGATGATCTGGTCGACTTCTTCAAGTGGACCAGCGAAGTCAACACCCAGAACTGGCCGCCCAAGGTTTCCGGC
TAAAAGGGGGAGCAAGAAAC

Downstream 100 bases:

>100_bases
GTCTTGGGATCGGGGCGGCCGCGTTGCGGCGGCCGCCCCTTTTTTCCGGAGAAATTCATGTCTGAATCCTTGCCCTTCTA
CCTGCCCCAAGGCAACGAAT

Product: nitric oxide reductase large subunit

Products: NA

Alternate protein names: NOR large subunit; Nitric oxide reductase cytochrome b subunit [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MKYESQKVALYFFAGALGLFVAQILFGLVIGTIVVMPNTLSELLPFNIARMIHTNALIVWLLMGFFGASYYLIPEEAETE
LYSTKLAIVQFWLFLIGAAAAVVGYLFHIHEGREFLEQPLWIKLAIVVVALMFLFNITMTVLKGRKTAITNVLLLGLWGI
AVFFLFSLYNPANLIMDKIFWWWVVHLWVEGVWELVMASILAFLMIKLTGVDREVVEKWLYVIIGLALFSGILGTGHHYY
WIGTPGYWQWIGSVFSTLEVAPFFAMVVFAFMMVWKGGRNHPNKAALLWSLGCSVFAFFGAGVWGFLHTLAPVNYITHGT
QVTAAHGHLAFYGAYVMINIAIMTYAMPNILKREPYNQLLNMWGFWITSSGVTFMTFALTFAGVVQSHLQRVMGMGFMEV
QDQLGLFYWMRLGAGVATFIGVVMIVWSLVVPGKAKPAQ

Sequences:

>Translated_439_residues
MKYESQKVALYFFAGALGLFVAQILFGLVIGTIVVMPNTLSELLPFNIARMIHTNALIVWLLMGFFGASYYLIPEEAETE
LYSTKLAIVQFWLFLIGAAAAVVGYLFHIHEGREFLEQPLWIKLAIVVVALMFLFNITMTVLKGRKTAITNVLLLGLWGI
AVFFLFSLYNPANLIMDKIFWWWVVHLWVEGVWELVMASILAFLMIKLTGVDREVVEKWLYVIIGLALFSGILGTGHHYY
WIGTPGYWQWIGSVFSTLEVAPFFAMVVFAFMMVWKGGRNHPNKAALLWSLGCSVFAFFGAGVWGFLHTLAPVNYITHGT
QVTAAHGHLAFYGAYVMINIAIMTYAMPNILKREPYNQLLNMWGFWITSSGVTFMTFALTFAGVVQSHLQRVMGMGFMEV
QDQLGLFYWMRLGAGVATFIGVVMIVWSLVVPGKAKPAQ
>Mature_439_residues
MKYESQKVALYFFAGALGLFVAQILFGLVIGTIVVMPNTLSELLPFNIARMIHTNALIVWLLMGFFGASYYLIPEEAETE
LYSTKLAIVQFWLFLIGAAAAVVGYLFHIHEGREFLEQPLWIKLAIVVVALMFLFNITMTVLKGRKTAITNVLLLGLWGI
AVFFLFSLYNPANLIMDKIFWWWVVHLWVEGVWELVMASILAFLMIKLTGVDREVVEKWLYVIIGLALFSGILGTGHHYY
WIGTPGYWQWIGSVFSTLEVAPFFAMVVFAFMMVWKGGRNHPNKAALLWSLGCSVFAFFGAGVWGFLHTLAPVNYITHGT
QVTAAHGHLAFYGAYVMINIAIMTYAMPNILKREPYNQLLNMWGFWITSSGVTFMTFALTFAGVVQSHLQRVMGMGFMEV
QDQLGLFYWMRLGAGVATFIGVVMIVWSLVVPGKAKPAQ

Specific function: Component of the anaerobic respiratory chain that transforms nitrate to dinitrogen (denitrification). NorB is the catalytic subunit of the enzyme complex. Shows proton pump activity across the membrane in denitrifying bacterial cells. The mononitrogen red

COG id: COG3256

COG function: function code P; Nitric oxide reductase large subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heme-copper respiratory oxidase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000883 [H]

Pfam domain/function: PF00115 COX1 [H]

EC number: =1.7.99.7 [H]

Molecular weight: Translated: 49437; Mature: 49437

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: PS50855 COX1 ; PS00077 COX1_CUB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYESQKVALYFFAGALGLFVAQILFGLVIGTIVVMPNTLSELLPFNIARMIHTNALIVW
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LLMGFFGASYYLIPEEAETELYSTKLAIVQFWLFLIGAAAAVVGYLFHIHEGREFLEQPL
HHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
WIKLAIVVVALMFLFNITMTVLKGRKTAITNVLLLGLWGIAVFFLFSLYNPANLIMDKIF
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
WWWVVHLWVEGVWELVMASILAFLMIKLTGVDREVVEKWLYVIIGLALFSGILGTGHHYY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
WIGTPGYWQWIGSVFSTLEVAPFFAMVVFAFMMVWKGGRNHPNKAALLWSLGCSVFAFFG
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
AGVWGFLHTLAPVNYITHGTQVTAAHGHLAFYGAYVMINIAIMTYAMPNILKREPYNQLL
HHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
NMWGFWITSSGVTFMTFALTFAGVVQSHLQRVMGMGFMEVQDQLGLFYWMRLGAGVATFI
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHH
GVVMIVWSLVVPGKAKPAQ
HHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKYESQKVALYFFAGALGLFVAQILFGLVIGTIVVMPNTLSELLPFNIARMIHTNALIVW
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LLMGFFGASYYLIPEEAETELYSTKLAIVQFWLFLIGAAAAVVGYLFHIHEGREFLEQPL
HHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
WIKLAIVVVALMFLFNITMTVLKGRKTAITNVLLLGLWGIAVFFLFSLYNPANLIMDKIF
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
WWWVVHLWVEGVWELVMASILAFLMIKLTGVDREVVEKWLYVIIGLALFSGILGTGHHYY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
WIGTPGYWQWIGSVFSTLEVAPFFAMVVFAFMMVWKGGRNHPNKAALLWSLGCSVFAFFG
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
AGVWGFLHTLAPVNYITHGTQVTAAHGHLAFYGAYVMINIAIMTYAMPNILKREPYNQLL
HHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
NMWGFWITSSGVTFMTFALTFAGVVQSHLQRVMGMGFMEVQDQLGLFYWMRLGAGVATFI
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHH
GVVMIVWSLVVPGKAKPAQ
HHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7711073; 10984043 [H]