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The map label for this gene is dctA1 [H]

Identifier: 83311989

GI number: 83311989

Start: 3088631

End: 3089389

Strand: Reverse

Name: dctA1 [H]

Synonym: amb2890

Alternate gene names: 83311989

Gene position: 3089389-3088631 (Counterclockwise)

Preceding gene: 83311990

Following gene: 83311986

Centisome position: 62.2

GC content: 64.03

Gene sequence:

>759_bases
ATGACCCATATGGGCCGGGCCGGTCAGGGCGTGCACATGTTCATCGAGGAATGCTCGCACATCTTCTTCGCCATGATGAA
CACCATCATGAAGGTCGCCCCCCTGGGCGCCGGCGGCGCCATGGCCTTTACCATCGGCAAGTACGGCGTGGCCGCGCTGA
AGCCGCTGGCGGCGCTGATGGGCAGCTTCTACCTGACCTGCTTCCTGTTCGTGGTGGTCGTGCTGGGCACCATCGCCCTG
CTAGTCGGCTTCAACATTTTCAAGTTCATCGCTTACATCAAGGAAGAGTTGCTGACCGTTCTCGGCACCTCGTCCTCGGA
ATCCGCCCTGGTGCCCCTGATGCGCAAGCTGGAGGCCATGGGCTGCTCCAAGTCGGTGGTCGGCCTGGTAGTTCCGTCGG
GGTATTCCTTCAACCTGGACGGCACCAACATCTACCTGACCATGGCGGCCCTGTTCGTGGCCCAGGCGCTGGACATCGAG
CTGACCTTCACCCAGCAGATCACCCTGCTCGGCGTGGCCATGCTGACCTCCAAGGGCGCGTCGGGCGTCACCGGCGCCGG
CTTCATCACCCTGGCCGCCACCCTGGCGGTGGTCCCCAGCGTGCCCGTCGCCGGTCTGGCGCTGATCCTGGGCATCGACC
GCTTCATGTCCGAGGCGCGGGCCCTGACCAATTTCGTGGGCAACGGCGTCGCCGCCGTGGTGGTCTCCAAGTGGGAGAAG
GAACTGGACCACGAGAAGATGAAGGAAGTCCTGGGCTAG

Upstream 100 bases:

>100_bases
CTTCATCCTGCACATCATCCCCAAGACCCTGGTGGATGCCTTCACCGGCGGCGGCGATCTGCTGCAGGTGCTGCTGGTGG
CCATCCTGTTCGGCTATGCC

Downstream 100 bases:

>100_bases
GCTGTCCCCCCTCTCCAGGACGAAGCTGGGCCCGTTCAGCCGACGCTGAACGGGCCCTTCTCTTATTCGGCCGCCAGATT
GGGGCGGGCGAGGGCGGCGC

Product: Na+/H+-dicarboxylate symporters

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MTHMGRAGQGVHMFIEECSHIFFAMMNTIMKVAPLGAGGAMAFTIGKYGVAALKPLAALMGSFYLTCFLFVVVVLGTIAL
LVGFNIFKFIAYIKEELLTVLGTSSSESALVPLMRKLEAMGCSKSVVGLVVPSGYSFNLDGTNIYLTMAALFVAQALDIE
LTFTQQITLLGVAMLTSKGASGVTGAGFITLAATLAVVPSVPVAGLALILGIDRFMSEARALTNFVGNGVAAVVVSKWEK
ELDHEKMKEVLG

Sequences:

>Translated_252_residues
MTHMGRAGQGVHMFIEECSHIFFAMMNTIMKVAPLGAGGAMAFTIGKYGVAALKPLAALMGSFYLTCFLFVVVVLGTIAL
LVGFNIFKFIAYIKEELLTVLGTSSSESALVPLMRKLEAMGCSKSVVGLVVPSGYSFNLDGTNIYLTMAALFVAQALDIE
LTFTQQITLLGVAMLTSKGASGVTGAGFITLAATLAVVPSVPVAGLALILGIDRFMSEARALTNFVGNGVAAVVVSKWEK
ELDHEKMKEVLG
>Mature_251_residues
THMGRAGQGVHMFIEECSHIFFAMMNTIMKVAPLGAGGAMAFTIGKYGVAALKPLAALMGSFYLTCFLFVVVVLGTIALL
VGFNIFKFIAYIKEELLTVLGTSSSESALVPLMRKLEAMGCSKSVVGLVVPSGYSFNLDGTNIYLTMAALFVAQALDIEL
TFTQQITLLGVAMLTSKGASGVTGAGFITLAATLAVVPSVPVAGLALILGIDRFMSEARALTNFVGNGVAAVVVSKWEKE
LDHEKMKEVLG

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI4827012, Length=260, Percent_Identity=31.9230769230769, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI169790839, Length=260, Percent_Identity=31.5384615384615, Blast_Score=98, Evalue=8e-21,
Organism=Homo sapiens, GI223468566, Length=239, Percent_Identity=31.3807531380753, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI223468564, Length=239, Percent_Identity=31.3807531380753, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI40254478, Length=255, Percent_Identity=29.8039215686275, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI5032093, Length=258, Percent_Identity=29.4573643410853, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI66773030, Length=247, Percent_Identity=30.3643724696356, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI194239697, Length=150, Percent_Identity=36.6666666666667, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI21314632, Length=267, Percent_Identity=25.8426966292135, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI262359914, Length=198, Percent_Identity=30.8080808080808, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI301601644, Length=153, Percent_Identity=31.3725490196078, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1789947, Length=251, Percent_Identity=64.9402390438247, Blast_Score=325, Evalue=1e-90,
Organism=Escherichia coli, GI1790514, Length=241, Percent_Identity=40.6639004149378, Blast_Score=185, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI71983106, Length=264, Percent_Identity=29.1666666666667, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71983099, Length=264, Percent_Identity=29.1666666666667, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17537407, Length=146, Percent_Identity=32.8767123287671, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI193206654, Length=153, Percent_Identity=30.718954248366, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71996953, Length=245, Percent_Identity=28.1632653061224, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24583025, Length=251, Percent_Identity=27.8884462151394, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI17137668, Length=251, Percent_Identity=27.8884462151394, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24583023, Length=251, Percent_Identity=27.8884462151394, Blast_Score=81, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 26545; Mature: 26414

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHMGRAGQGVHMFIEECSHIFFAMMNTIMKVAPLGAGGAMAFTIGKYGVAALKPLAALM
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEHHHHHHHHHHHHHHHH
GSFYLTCFLFVVVVLGTIALLVGFNIFKFIAYIKEELLTVLGTSSSESALVPLMRKLEAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
GCSKSVVGLVVPSGYSFNLDGTNIYLTMAALFVAQALDIELTFTQQITLLGVAMLTSKGA
CCCCCCEEEEECCCEEECCCCCHHHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHCCCC
SGVTGAGFITLAATLAVVPSVPVAGLALILGIDRFMSEARALTNFVGNGVAAVVVSKWEK
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ELDHEKMKEVLG
HHHHHHHHHHCC
>Mature Secondary Structure 
THMGRAGQGVHMFIEECSHIFFAMMNTIMKVAPLGAGGAMAFTIGKYGVAALKPLAALM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEHHHHHHHHHHHHHHHH
GSFYLTCFLFVVVVLGTIALLVGFNIFKFIAYIKEELLTVLGTSSSESALVPLMRKLEAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
GCSKSVVGLVVPSGYSFNLDGTNIYLTMAALFVAQALDIELTFTQQITLLGVAMLTSKGA
CCCCCCEEEEECCCEEECCCCCHHHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHCCCC
SGVTGAGFITLAATLAVVPSVPVAGLALILGIDRFMSEARALTNFVGNGVAAVVVSKWEK
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ELDHEKMKEVLG
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]