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The map label for this gene is paaJ [H]

Identifier: 83311694

GI number: 83311694

Start: 2781078

End: 2782274

Strand: Reverse

Name: paaJ [H]

Synonym: amb2595

Alternate gene names: 83311694

Gene position: 2782274-2781078 (Counterclockwise)

Preceding gene: 83311695

Following gene: 83311693

Centisome position: 56.01

GC content: 70.01

Gene sequence:

>1197_bases
ATGCTTGACGCCTATATCTACGACGGCCTGCGTTCCCCCATCGGCCGCCATGGCGGCGGCCTCGCCCCGGTGCGTTCCGA
CGATCTGGCCGCCGAGGTGATCCGCGCCCTGGTGGCCCGCTCATCGTTCAAGCCCGAAGATGTCGAGGACGTGATTCTCG
GCTGCACCAACCAGGCGGGCGAGGACAGCCGCAACGTGGCCCGTCATGCCGCCTTGCTGGCCGGTCTGCCGGTGGAGGTG
GCGGGCCAGACCGTCAACCGCCTGTGCGCCTCGGGACTGGCCGCCGTGCTGGACGCGGCGCGTTCGGTGACCTGCGGCGA
GGGTGACCTGTATCTGGCCGGCGGTGTCGAGAGCATGACCCGCGCCCCCTTCGTGCTGGCCAAGGGCGACAGCGCCTGGT
CCCGCGATGCCAGGATCTTCGACACCACCATCGGGGCGCGTTTCGCCAATCCCAAGGTGGTGAAGTCCTTCGGCGGCCAT
TCCATGCCGGAGACCGCCGACAACATCGCCCATGACCTGGGCCTGTCGCGCGAAGCCTCGGATGCCTTCGCCGCCGCCTC
CCAGGCCAAGTACGCCAAGGCCAAGGCCGAAGGCTTCTACGAGGGCGAGATCCACCCCATCACCATCGCCGGCCGCAAGG
GCGACACCATCGTGGCCGAGGACGAGCATCCGCGTCCCCAGACCGATCTTGCCGCGCTGACCAAGCTGAAGCCTTTGTTC
GAGGGCGGCGTGGTCACCGCCGGCAATGCGTCGGGCATCAATGACGGCGCTGCGGCGCTGTTCATCGGCTCCCGCGCCGC
CGGCGAGAAGGCGGGGATCGCCCCCATCGCCCGCATCGTCGCCGGCGCGGCGGCCGGCGTGCCGCCGCGTGTCATGGGCC
TGGGGCCGGTTCCCGCCATCACCAAGGCCCTGGCGCGGGCCAAGCTTTCCCTCAAGGACCTCGACCTCATCGAGATCAAC
GAGGCCTTCGCCGTGCAGGTGCTGGGTTGCGTCACCCAACTGGGCGTGGCCGCCGATGATTCCCGCCTCAATCCCAATGG
CGGCGCCATCGCCATCGGCCATCCGCTGGGCTGCTCCGGTGCCCGTCTGGCGCTGACGGCGGCGCGGCAGTTGCAGCGGA
CCGGCGGGCGCCATGCCGTGGTCTCCTTGTGCATCGGCGTCGGCCACGGCCTCGCCGCCGTGATCGAAAGGGTCTGA

Upstream 100 bases:

>100_bases
CGTGCTGGCCATCACCGGCGATCCCCGCTACCGCGCCAGCCTGTGGCTGAAACGCCGCGCCGCTCTAGGCGTCTCTTTGC
TGACTTCGGAGTCCTGATCC

Downstream 100 bases:

>100_bases
TCCATGGCCATCAAGGAAATCCTCGTCCATGTGGAGCCCGACGCAGCCGGGGCGGGGCGTCTCGACCTCGCCCGAATGCT
GGCGGCCGAGCACGGCGCCC

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MLDAYIYDGLRSPIGRHGGGLAPVRSDDLAAEVIRALVARSSFKPEDVEDVILGCTNQAGEDSRNVARHAALLAGLPVEV
AGQTVNRLCASGLAAVLDAARSVTCGEGDLYLAGGVESMTRAPFVLAKGDSAWSRDARIFDTTIGARFANPKVVKSFGGH
SMPETADNIAHDLGLSREASDAFAAASQAKYAKAKAEGFYEGEIHPITIAGRKGDTIVAEDEHPRPQTDLAALTKLKPLF
EGGVVTAGNASGINDGAAALFIGSRAAGEKAGIAPIARIVAGAAAGVPPRVMGLGPVPAITKALARAKLSLKDLDLIEIN
EAFAVQVLGCVTQLGVAADDSRLNPNGGAIAIGHPLGCSGARLALTAARQLQRTGGRHAVVSLCIGVGHGLAAVIERV

Sequences:

>Translated_398_residues
MLDAYIYDGLRSPIGRHGGGLAPVRSDDLAAEVIRALVARSSFKPEDVEDVILGCTNQAGEDSRNVARHAALLAGLPVEV
AGQTVNRLCASGLAAVLDAARSVTCGEGDLYLAGGVESMTRAPFVLAKGDSAWSRDARIFDTTIGARFANPKVVKSFGGH
SMPETADNIAHDLGLSREASDAFAAASQAKYAKAKAEGFYEGEIHPITIAGRKGDTIVAEDEHPRPQTDLAALTKLKPLF
EGGVVTAGNASGINDGAAALFIGSRAAGEKAGIAPIARIVAGAAAGVPPRVMGLGPVPAITKALARAKLSLKDLDLIEIN
EAFAVQVLGCVTQLGVAADDSRLNPNGGAIAIGHPLGCSGARLALTAARQLQRTGGRHAVVSLCIGVGHGLAAVIERV
>Mature_398_residues
MLDAYIYDGLRSPIGRHGGGLAPVRSDDLAAEVIRALVARSSFKPEDVEDVILGCTNQAGEDSRNVARHAALLAGLPVEV
AGQTVNRLCASGLAAVLDAARSVTCGEGDLYLAGGVESMTRAPFVLAKGDSAWSRDARIFDTTIGARFANPKVVKSFGGH
SMPETADNIAHDLGLSREASDAFAAASQAKYAKAKAEGFYEGEIHPITIAGRKGDTIVAEDEHPRPQTDLAALTKLKPLF
EGGVVTAGNASGINDGAAALFIGSRAAGEKAGIAPIARIVAGAAAGVPPRVMGLGPVPAITKALARAKLSLKDLDLIEIN
EAFAVQVLGCVTQLGVAADDSRLNPNGGAIAIGHPLGCSGARLALTAARQLQRTGGRHAVVSLCIGVGHGLAAVIERV

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=398, Percent_Identity=40.4522613065327, Blast_Score=284, Evalue=9e-77,
Organism=Homo sapiens, GI148539872, Length=396, Percent_Identity=39.1414141414141, Blast_Score=261, Evalue=6e-70,
Organism=Homo sapiens, GI4501853, Length=402, Percent_Identity=39.0547263681592, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI4557237, Length=399, Percent_Identity=32.8320802005013, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI4504327, Length=424, Percent_Identity=30.8962264150943, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI194353979, Length=397, Percent_Identity=29.7229219143577, Blast_Score=141, Evalue=1e-33,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=57.1072319201995, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1788554, Length=401, Percent_Identity=42.643391521197, Blast_Score=275, Evalue=3e-75,
Organism=Escherichia coli, GI48994986, Length=409, Percent_Identity=39.6088019559902, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI87082165, Length=407, Percent_Identity=39.0663390663391, Blast_Score=255, Evalue=4e-69,
Organism=Escherichia coli, GI1788683, Length=410, Percent_Identity=30.4878048780488, Blast_Score=159, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI133906874, Length=400, Percent_Identity=38.25, Blast_Score=264, Evalue=5e-71,
Organism=Caenorhabditis elegans, GI25147385, Length=398, Percent_Identity=34.1708542713568, Blast_Score=211, Evalue=7e-55,
Organism=Caenorhabditis elegans, GI17535921, Length=400, Percent_Identity=33.75, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17551802, Length=433, Percent_Identity=31.4087759815243, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17535917, Length=408, Percent_Identity=29.9019607843137, Blast_Score=152, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6322031, Length=409, Percent_Identity=35.4523227383863, Blast_Score=214, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6325229, Length=406, Percent_Identity=34.9753694581281, Blast_Score=206, Evalue=6e-54,
Organism=Drosophila melanogaster, GI24655093, Length=399, Percent_Identity=39.0977443609023, Blast_Score=278, Evalue=4e-75,
Organism=Drosophila melanogaster, GI17648125, Length=403, Percent_Identity=39.454094292804, Blast_Score=265, Evalue=3e-71,
Organism=Drosophila melanogaster, GI24640423, Length=395, Percent_Identity=36.2025316455696, Blast_Score=221, Evalue=6e-58,
Organism=Drosophila melanogaster, GI17137578, Length=422, Percent_Identity=31.042654028436, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 40706; Mature: 40706

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDAYIYDGLRSPIGRHGGGLAPVRSDDLAAEVIRALVARSSFKPEDVEDVILGCTNQAG
CCCHHHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCC
EDSRNVARHAALLAGLPVEVAGQTVNRLCASGLAAVLDAARSVTCGEGDLYLAGGVESMT
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHH
RAPFVLAKGDSAWSRDARIFDTTIGARFANPKVVKSFGGHSMPETADNIAHDLGLSREAS
CCCEEEECCCCCCCCCCEEEEHHHCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCHH
DAFAAASQAKYAKAKAEGFYEGEIHPITIAGRKGDTIVAEDEHPRPQTDLAALTKLKPLF
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHH
EGGVVTAGNASGINDGAAALFIGSRAAGEKAGIAPIARIVAGAAAGVPPRVMGLGPVPAI
CCCEEECCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCHHCCCCCHHHH
TKALARAKLSLKDLDLIEINEAFAVQVLGCVTQLGVAADDSRLNPNGGAIAIGHPLGCSG
HHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCC
ARLALTAARQLQRTGGRHAVVSLCIGVGHGLAAVIERV
CHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MLDAYIYDGLRSPIGRHGGGLAPVRSDDLAAEVIRALVARSSFKPEDVEDVILGCTNQAG
CCCHHHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCC
EDSRNVARHAALLAGLPVEVAGQTVNRLCASGLAAVLDAARSVTCGEGDLYLAGGVESMT
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHH
RAPFVLAKGDSAWSRDARIFDTTIGARFANPKVVKSFGGHSMPETADNIAHDLGLSREAS
CCCEEEECCCCCCCCCCEEEEHHHCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCHH
DAFAAASQAKYAKAKAEGFYEGEIHPITIAGRKGDTIVAEDEHPRPQTDLAALTKLKPLF
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHH
EGGVVTAGNASGINDGAAALFIGSRAAGEKAGIAPIARIVAGAAAGVPPRVMGLGPVPAI
CCCEEECCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCHHCCCCCHHHH
TKALARAKLSLKDLDLIEINEAFAVQVLGCVTQLGVAADDSRLNPNGGAIAIGHPLGCSG
HHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCC
ARLALTAARQLQRTGGRHAVVSLCIGVGHGLAAVIERV
CHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 10766858 [H]